(Circular) (Six-base) MAPSORT of: pFN21AA0933.seq Check: 9744 from: 1 to: 11446 pFN21AA0933 11446 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4278 5019 6178 6662 4278 Size: 741 1159 484 9062 Fragments arranged by size: 9062 1159 741 484 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9256 278 Size: 53 83 186 936 7720 2468 Fragments arranged by size: 7720 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4299 7011 8789 1937 Size: 2362 2712 1778 4594 Fragments arranged by size: 4594 2712 2362 1778 Acc65I G'GTAC_C Cuts at: 8767 8767 Size: 11446 AclI AA'CG_TT Cuts at: 1563 9573 9946 1563 Size: 8010 373 3063 Fragments arranged by size: 8010 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2043 2132 3107 3776 4406 5022 6197 7482 Size: 411 89 975 669 630 616 1175 1285 Cuts at: 7482 7948 9509 10893 11161 1632 Size: 466 1561 1384 268 1917 Fragments arranged by size: 1917 1561 1384 1285 1175 975 669 630 616 466 411 268 89 AfeI AGC'GCT Cuts at: 5324 6053 8328 5324 Size: 729 2275 8442 Fragments arranged by size: 8442 2275 729 AflII C'TTAA_G Cuts at: 820 1017 3562 820 Size: 197 2545 8704 Fragments arranged by size: 8704 2545 197 AflIII A'CryG_T Cuts at: 1203 2871 3596 5223 5763 10345 11322 1203 Size: 1668 725 1627 540 4582 977 1327 Fragments arranged by size: 4582 1668 1627 1327 977 725 540 AgeI A'CCGG_T Cuts at: 10277 10277 Size: 11446 AhdI GACnn_n'nnGTC Cuts at: 2974 3649 6618 10175 2974 Size: 675 2969 3557 4245 Fragments arranged by size: 4245 3557 2969 675 AleI CACnn'nnGTG Cuts at: 1166 5033 1166 Size: 3867 7579 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3558 4347 6104 1184 Size: 2374 789 1757 6526 Fragments arranged by size: 6526 2374 1757 789 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3655 3687 4833 4865 5213 5245 3655 Size: 32 1146 32 348 32 9856 Fragments arranged by size: 9856 1146 348 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2419 2945 4015 4090 4483 5263 6193 6920 7714 Size: 526 1070 75 393 780 930 727 794 Cuts at: 7714 9189 10761 2419 Size: 1475 1572 3104 Fragments arranged by size: 3104 1572 1475 1070 930 794 780 727 526 393 75 ApaI G_GGCC'C Cuts at: 6088 6226 8408 6088 Size: 138 2182 9126 Fragments arranged by size: 9126 2182 138 ApaLI G'TGCA_C Cuts at: 5286 7023 9503 10659 5286 Size: 1737 2480 1156 6073 Fragments arranged by size: 6073 2480 1737 1156 ApoI r'AATT_y Cuts at: 1493 2083 2956 3151 3241 3301 5621 6864 8312 Size: 590 873 195 90 60 2320 1243 1448 Cuts at: 8312 8364 8466 8534 8755 8981 9133 11242 1493 Size: 52 102 68 221 226 152 2109 1697 Fragments arranged by size: 2320 2109 1697 1448 1243 873 590 226 221 195 152 102 90 68 60 52 AseI AT'TA_AT Cuts at: 160 1032 10000 160 Size: 872 8968 1606 Fragments arranged by size: 8968 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11446 AvaI C'yCGr_G Cuts at: 1870 1943 2095 3667 3825 4817 4979 5075 6258 Size: 73 152 1572 158 992 162 96 1183 Cuts at: 6258 8771 1870 Size: 2513 4545 Fragments arranged by size: 4545 2513 1572 1183 992 162 158 152 96 73 AvrII C'CTAG_G Cuts at: 6802 6802 Size: 11446 BamHI G'GATC_C Cuts at: 2008 6818 8776 9143 2008 Size: 4810 1958 367 4311 Fragments arranged by size: 4810 4311 1958 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4769 5156 8767 10222 618 Size: 325 217 627 2982 387 3611 1455 1842 Fragments arranged by size: 3611 2982 1842 1455 627 387 325 217 BanII G_rGCy'C Cuts at: 1391 2028 2480 3438 3783 3804 4056 4526 5076 Size: 637 452 958 345 21 252 470 550 Cuts at: 5076 5457 6088 6226 6277 7721 8408 8765 1391 Size: 381 631 138 51 1444 687 357 4072 Fragments arranged by size: 4072 1444 958 687 637 631 550 470 452 381 357 345 252 138 51 21 BbeI G_GCGC'C Cuts at: 5160 5160 Size: 11446 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2599 2893 2973 3445 3599 4146 4506 Size: 971 700 294 80 472 154 547 360 Cuts at: 4506 5937 6335 7503 7954 928 Size: 1431 398 1168 451 4420 Fragments arranged by size: 4420 1431 1168 971 700 547 472 451 398 360 294 154 80 BbvCI CC'TCA_GC Cuts at: 3546 7467 3546 Size: 3921 7525 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3962 3996 9637 9671 1903 Size: 34 2025 34 5641 34 3678 Fragments arranged by size: 5641 3678 2025 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5647 7798 9338 10548 5647 Size: 2151 1540 1210 6545 Fragments arranged by size: 6545 2151 1540 1210 BclI T'GATC_A Cuts at: 1548 5907 7978 1548 Size: 4359 2071 5016 Fragments arranged by size: 5016 4359 2071 BfrBI ATG'CAT Cuts at: 2897 3080 6397 8712 2897 Size: 183 3317 2315 5631 Fragments arranged by size: 5631 3317 2315 183 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2182 5054 5336 Size: 107 122 71 631 605 510 2872 282 Cuts at: 5336 5516 10057 136 Size: 180 4541 1525 Fragments arranged by size: 4541 2872 1525 631 605 510 282 180 122 107 71 BglII A'GATC_T Cuts at: 2484 2816 6141 8176 11441 2484 Size: 332 3325 2035 3265 2489 Fragments arranged by size: 3325 3265 2489 2035 332 BlpI GC'TnA_GC Cuts at: 3611 5102 5264 7409 7707 7715 8863 3611 Size: 1491 162 2145 298 8 1148 6194 Fragments arranged by size: 6194 2145 1491 1148 298 162 8 Bme1580I G_kGCm'C Cuts at: 1790 4980 5290 5771 6088 6226 7027 8408 9507 Size: 3190 310 481 317 138 801 1381 1099 Cuts at: 9507 10663 1790 Size: 1156 2573 Fragments arranged by size: 3190 2573 1381 1156 1099 801 481 317 310 138 BmgBI CAC'GTC Cuts at: 1318 3061 3597 4825 5226 5881 1318 Size: 1743 536 1228 401 655 6883 Fragments arranged by size: 6883 1743 1228 655 536 401 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2226 2424 3496 Size: 416 53 83 399 24 809 198 1072 Cuts at: 3496 4471 7166 9177 10135 442 Size: 975 2695 2011 958 1753 Fragments arranged by size: 2695 2011 1753 1072 975 958 809 416 399 198 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11446 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3840 5014 5443 5882 3840 Size: 1174 429 439 9404 Fragments arranged by size: 9404 1174 439 429 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4415 4566 5413 5441 5612 5729 5807 6166 Size: 3176 151 847 28 171 117 78 359 Cuts at: 6166 6561 7774 7790 10106 1239 Size: 395 1213 16 2316 2579 Fragments arranged by size: 3176 2579 2316 1213 847 395 359 171 151 117 78 28 16 Bpu10I CC'TnA_GC Cuts at: 3546 5810 7467 3546 Size: 2264 1657 7525 Fragments arranged by size: 7525 2264 1657 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3123 3835 4883 6709 7897 9571 10436 10734 3123 Size: 712 1048 1826 1188 1674 865 298 3835 Fragments arranged by size: 3835 1826 1674 1188 1048 865 712 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3433 4122 4310 5854 5935 6355 6781 Size: 617 1934 689 188 1544 81 420 426 Cuts at: 6781 8143 10109 882 Size: 1362 1966 2219 Fragments arranged by size: 2219 1966 1934 1544 1362 689 617 426 420 188 81 BsaAI yAC'GTr Cuts at: 493 1204 5128 6313 9237 493 Size: 711 3924 1185 2924 2702 Fragments arranged by size: 3924 2924 2702 1185 711 BsaBI GATnn'nnATC Cuts at: 4620 11440 4620 Size: 6820 4626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5157 6029 9253 9635 Size: 53 83 186 936 3624 872 3224 382 Cuts at: 9635 10270 275 Size: 635 1451 Fragments arranged by size: 3624 3224 1451 936 872 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3796 9879 10277 10551 10698 11109 3796 Size: 6083 398 274 147 411 4133 Fragments arranged by size: 6083 4133 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2639 2669 4555 4585 4700 Size: 30 165 30 1236 30 1886 30 115 Cuts at: 4700 4730 4833 4863 5213 5243 5669 5699 7282 Size: 30 103 30 350 30 426 30 1583 Cuts at: 7282 7312 7463 7493 1178 Size: 30 151 30 5131 Fragments arranged by size: 5131 1886 1583 1236 426 350 165 151 115 103 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2801 3989 5227 5453 5684 6125 6245 Size: 991 624 1188 1238 226 231 441 120 Cuts at: 6245 6788 7484 8094 8444 1186 Size: 543 696 610 350 4188 Fragments arranged by size: 4188 1238 1188 991 696 624 610 543 441 350 231 226 120 BseYI C'CCAG_C Cuts at: 2662 4160 5366 6951 6996 7688 10649 11145 2662 Size: 1498 1206 1585 45 692 2961 496 2963 Fragments arranged by size: 2963 2961 1585 1498 1206 692 496 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3554 4744 5903 6335 7040 1163 Size: 573 1818 1190 1159 432 705 5569 Fragments arranged by size: 5569 1818 1190 1159 705 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2644 4917 8896 9657 10685 1540 Size: 275 186 643 2273 3979 761 1028 2301 Fragments arranged by size: 3979 2301 2273 1028 761 643 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 3438 3522 3612 3804 3813 4526 4851 5010 Size: 1410 84 90 192 9 713 325 159 Cuts at: 5010 5178 5290 5457 5604 6156 7027 7721 9507 Size: 168 112 167 147 552 871 694 1786 Cuts at: 9507 9592 10663 2028 Size: 85 1071 2811 Fragments arranged by size: 2811 1786 1410 1071 871 713 694 552 325 192 168 167 159 147 112 90 85 84 9 BsmI GAATG_Cn' Cuts at: 2832 3371 6424 6700 8450 8714 8957 9050 2832 Size: 539 3053 276 1750 264 243 93 5228 Fragments arranged by size: 5228 3053 1750 539 276 264 243 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2480 3438 3522 3612 3783 3804 Size: 399 238 452 958 84 90 171 21 Cuts at: 3804 3813 4056 4526 4851 4980 5010 5076 5178 Size: 9 243 470 325 129 30 66 102 Cuts at: 5178 5290 5457 5604 5771 6088 6156 6226 6277 Size: 112 167 147 167 317 68 70 51 Cuts at: 6277 7027 7721 8408 8765 9507 9592 10663 1391 Size: 750 694 687 357 742 85 1071 2174 Fragments arranged by size: 2174 1071 958 750 742 694 687 470 452 399 357 325 317 243 238 171 167 167 147 129 112 102 90 85 84 70 68 66 51 30 21 9 BspEI T'CCGG_A Cuts at: 11109 11109 Size: 11446 BspHI T'CATG_A Cuts at: 4395 7852 8592 9335 11065 4395 Size: 3457 740 743 1730 4776 Fragments arranged by size: 4776 3457 1730 743 740 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4278 5019 5262 5553 5875 6178 6662 7341 Size: 3434 741 243 291 322 303 484 679 Cuts at: 7341 8801 844 Size: 1460 3489 Fragments arranged by size: 3489 3434 1460 741 679 484 322 303 291 243 BsrBI CCG'CTC Cuts at: 1394 1823 4995 9333 1394 Size: 429 3172 4338 3507 Fragments arranged by size: 4338 3507 3172 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4575 4599 7374 7509 9941 10115 65 Size: 1183 3327 24 2775 135 2432 174 1396 Fragments arranged by size: 3327 2775 2432 1396 1183 174 135 24 BsrFI r'CCGG_y Cuts at: 1700 1810 2371 4358 7379 10090 10277 1700 Size: 110 561 1987 3021 2711 187 2869 Fragments arranged by size: 3021 2869 2711 1987 561 187 110 BsrGI T'GTAC_A Cuts at: 96 2319 6036 7228 7530 96 Size: 2223 3717 1192 302 4012 Fragments arranged by size: 4012 3717 2223 1192 302 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11446 BssSI C'ACGA_G Cuts at: 4110 6040 9506 10518 4110 Size: 1930 3466 1012 5038 Fragments arranged by size: 5038 3466 1930 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2419 5536 6160 6313 6430 8581 794 Size: 1625 3117 624 153 117 2151 3659 Fragments arranged by size: 3659 3117 2151 1625 624 153 117 BstBI TT'CG_AA Cuts at: 10250 10250 Size: 11446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 11446 BstXI CCAn_nnnn'nTGG Cuts at: 2299 4456 5299 2299 Size: 2157 843 8446 Fragments arranged by size: 8446 2157 843 BstYI r'GATC_y Cuts at: 1972 2008 2110 2484 2816 3004 4683 6141 6818 Size: 36 102 374 332 188 1679 1458 677 Cuts at: 6818 8176 8688 8776 9143 9528 9545 10997 11083 Size: 1358 512 88 367 385 17 1452 86 Cuts at: 11083 11095 11441 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1679 1458 1452 1358 677 512 385 374 367 346 332 188 102 88 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2159 3513 4986 5616 7440 2159 Size: 1354 1473 630 1824 6165 Fragments arranged by size: 6165 1824 1473 1354 630 BtgI C'CryG_G Cuts at: 513 1065 2004 3423 5152 5298 6076 6663 6741 Size: 552 939 1419 1729 146 778 587 78 Cuts at: 6741 513 Size: 5218 Fragments arranged by size: 5218 1729 1419 939 778 587 552 146 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 10707 Fragments arranged by size: 10707 683 56 BtsI GCAGTG_nn' Cuts at: 2408 3723 4891 4980 6936 8962 9756 9776 2408 Size: 1315 1168 89 1956 2026 794 20 4078 Fragments arranged by size: 4078 2026 1956 1315 1168 794 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11411 DraI TTT'AAA Cuts at: 2278 6838 8750 2278 Size: 4560 1912 4974 Fragments arranged by size: 4974 4560 1912 DraIII CAC_nnn'GTG Cuts at: 7063 7063 Size: 11446 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5885 8669 10453 809 Size: 507 4569 2784 1784 1802 Fragments arranged by size: 4569 2784 1802 1784 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2574 2641 Size: 54 1425 50 275 18 471 273 67 Cuts at: 2641 4440 4914 5203 5722 5937 6063 8126 8893 Size: 1799 474 289 519 215 126 2063 767 Cuts at: 8893 9782 11411 8 Size: 889 1629 43 Fragments arranged by size: 2063 1799 1629 1425 889 767 519 474 471 289 275 273 215 126 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2641 4914 8893 1537 Size: 275 829 2273 3979 4090 Fragments arranged by size: 4090 3979 2273 829 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 2062 2151 2466 3983 4308 4346 4998 Size: 657 52 89 315 1517 325 38 652 Cuts at: 4998 5106 5268 6711 6829 7073 7390 8522 9376 Size: 108 162 1443 118 244 317 1132 854 Cuts at: 9376 1353 Size: 3423 Fragments arranged by size: 3423 1517 1443 1132 854 657 652 325 317 315 244 162 118 108 89 52 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2123 2579 5459 6251 7507 10033 Size: 1064 138 534 456 2880 792 1256 2526 Cuts at: 10033 10407 10553 387 Size: 374 146 1280 Fragments arranged by size: 2880 2526 1280 1256 1064 792 534 456 374 146 138 EcoICRI GAG'CTC Cuts at: 2026 3436 3802 4524 5455 7719 2026 Size: 1410 366 722 931 2264 5753 Fragments arranged by size: 5753 2264 1410 931 722 366 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2132 3107 3776 4406 4415 4566 Size: 393 411 89 975 669 630 9 151 Cuts at: 4566 5022 5413 5441 5612 5729 5807 6166 6197 Size: 456 391 28 171 117 78 359 31 Cuts at: 6197 6561 7482 7774 7790 7948 9509 10106 10893 Size: 364 921 292 16 158 1561 597 787 Cuts at: 10893 11161 1239 Size: 268 1524 Fragments arranged by size: 1561 1524 975 921 787 669 630 597 456 411 393 391 364 359 292 268 171 158 151 117 89 78 31 28 16 9 EcoNI CCTnn'n_nnAGG Cuts at: 2471 4307 4723 4901 6092 6107 6559 7064 7559 Size: 1836 416 178 1191 15 452 505 495 Cuts at: 7559 2471 Size: 6358 Fragments arranged by size: 6358 1836 1191 505 495 452 416 178 15 EcoO109I rG'GnC_Cy Cuts at: 3134 3174 4728 5461 5569 5713 5755 6084 6085 Size: 40 1554 733 108 144 42 329 1 Cuts at: 6085 7474 7765 8404 8405 3134 Size: 1389 291 639 1 6175 Fragments arranged by size: 6175 1554 1389 733 639 329 291 144 108 42 40 1 1 EcoRI G'AATT_C Cuts at: 5621 8466 8534 8755 9133 5621 Size: 2845 68 221 378 7934 Fragments arranged by size: 7934 2845 378 221 68 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2073 2073 Size: 11446 FspI TGC'GCA Cuts at: 2831 4972 9952 2831 Size: 2141 4980 4325 Fragments arranged by size: 4980 4325 2141 FspAI rTGC'GCAy Cuts at: 2831 2831 Size: 11446 HaeII r_GCGC'y Cuts at: 1678 4490 5160 5326 6055 6303 7707 7948 8330 Size: 2812 670 166 729 248 1404 241 382 Cuts at: 8330 10593 1678 Size: 2263 2531 Fragments arranged by size: 2812 2531 2263 1404 729 670 382 248 241 166 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2172 2204 2808 Size: 32 135 32 530 32 38 32 604 Cuts at: 2808 2840 2960 2992 3022 3054 3301 3333 3635 Size: 32 120 32 30 32 247 32 302 Cuts at: 3635 3667 4133 4165 4555 4587 4654 4686 5183 Size: 32 466 32 390 32 67 32 497 Cuts at: 5183 5210 5215 5242 5621 5645 5653 5677 6604 Size: 27 5 27 379 24 8 24 927 Cuts at: 6604 6636 6880 6912 7097 7129 8011 8043 10087 Size: 32 244 32 185 32 882 32 2044 Cuts at: 10087 10119 10161 10193 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2044 927 882 604 530 497 466 390 379 302 247 244 185 135 120 67 42 38 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 27 27 24 24 8 5 HincII GTy'rAC Cuts at: 669 1657 1938 6736 6912 7012 8790 9036 669 Size: 988 281 4798 176 100 1778 246 3079 Fragments arranged by size: 4798 3079 1778 988 281 246 176 100 HindIII A'AGCT_T Cuts at: 748 5356 748 Size: 4608 6838 HpaI GTT'AAC Cuts at: 6736 9036 6736 Size: 2300 9146 KasI G'GCGC_C Cuts at: 5156 5156 Size: 11446 KpnI G_GTAC'C Cuts at: 8771 8771 Size: 11446 MfeI C'AATT_G Cuts at: 9045 9045 Size: 11446 MluI A'CGCG_T Cuts at: 11322 11322 Size: 11446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2514 2691 5712 8017 8400 10560 10744 1305 Size: 1209 177 3021 2305 383 2160 184 2007 Fragments arranged by size: 3021 2305 2160 2007 1209 383 184 177 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 2576 4442 5724 5939 Size: 54 1425 343 471 273 1866 1282 215 Cuts at: 5939 6065 8128 10 Size: 126 2063 3328 Fragments arranged by size: 3328 2063 1866 1425 1282 471 343 273 215 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 4067 5033 5297 7133 9404 9763 9922 Size: 648 2901 966 264 1836 2271 359 159 Cuts at: 9922 518 Size: 2042 Fragments arranged by size: 2901 2271 2042 1836 966 648 359 264 159 MspA1I CmG'CkG Cuts at: 1606 2325 3660 3943 4348 5089 5494 5926 6160 Size: 719 1335 283 405 741 405 432 234 Cuts at: 6160 6917 7404 7711 8629 8862 9539 10687 10932 Size: 757 487 307 918 233 677 1148 245 Cuts at: 10932 1606 Size: 2120 Fragments arranged by size: 2120 1335 1148 918 757 741 719 677 487 432 405 405 307 283 245 234 233 NaeI GCC'GGC Cuts at: 7381 7381 Size: 11446 NarI GG'CG_CC Cuts at: 5157 5157 Size: 11446 NcoI C'CATG_G Cuts at: 513 1065 2004 5298 6663 6741 513 Size: 552 939 3294 1365 78 5218 Fragments arranged by size: 5218 3294 1365 939 552 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 11446 NgoMIV G'CCGG_C Cuts at: 7379 7379 Size: 11446 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11446 NotI GC'GGCC_GC Cuts at: 8893 8893 Size: 11446 NsiI A_TGCA'T Cuts at: 2899 3082 6399 8714 2899 Size: 183 3317 2315 5631 Fragments arranged by size: 5631 3317 2315 183 NspI r_CATG'y Cuts at: 1132 1229 2662 2875 3587 3874 4971 6397 7276 Size: 97 1433 213 712 287 1097 1426 879 Cuts at: 7276 7888 8804 10349 1132 Size: 612 916 1545 2229 Fragments arranged by size: 2229 1545 1433 1426 1097 916 879 712 612 287 213 97 PasI CC'CwG_GG Cuts at: 1348 4789 6337 6451 1348 Size: 3441 1548 114 6343 Fragments arranged by size: 6343 3441 1548 114 PciI A'CATG_T Cuts at: 2871 10345 2871 Size: 7474 3972 PflMI CCAn_nnn'nTGG Cuts at: 1108 4438 4483 1108 Size: 3330 45 8071 Fragments arranged by size: 8071 3330 45 PfoI T'CCnGG_A Cuts at: 5639 5784 7578 7767 8241 5639 Size: 145 1794 189 474 8844 Fragments arranged by size: 8844 1794 474 189 145 PmeI GTTT'AAAC Cuts at: 8750 8750 Size: 11446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2488 2520 4833 4865 5213 5245 5507 5539 9515 Size: 32 2313 32 348 32 262 32 3976 Cuts at: 9515 9547 11028 11060 2488 Size: 32 1481 32 2874 Fragments arranged by size: 3976 2874 2313 1481 348 262 32 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4728 5569 5755 7474 7765 4728 Size: 841 186 1719 291 8409 Fragments arranged by size: 8409 1719 841 291 186 PshAI GACnn'nnGTC Cuts at: 1613 5753 1613 Size: 4140 7306 PsiI TTA'TAA Cuts at: 9016 9016 Size: 11446 PspOMI G'GGCC_C Cuts at: 6084 6222 8404 6084 Size: 138 2182 9126 Fragments arranged by size: 9126 2182 138 PspXI vC'TCGA_Gb Cuts at: 2095 3667 3825 2095 Size: 1572 158 9716 Fragments arranged by size: 9716 1572 158 PstI C_TGCA'G Cuts at: 830 2415 2439 3194 3985 4557 4866 5191 5259 Size: 1585 24 755 791 572 309 325 68 Cuts at: 5259 5550 5988 6165 6189 6309 6375 6673 7041 Size: 291 438 177 24 120 66 298 368 Cuts at: 7041 7338 7495 8798 830 Size: 297 157 1303 3478 Fragments arranged by size: 3478 1585 1303 791 755 572 438 368 325 309 298 297 291 177 157 120 68 66 24 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11446 PvuII CAG'CTG Cuts at: 2325 3943 4348 5926 7404 7711 8629 2325 Size: 1618 405 1578 1478 307 918 5142 Fragments arranged by size: 5142 1618 1578 1478 918 405 307 SacI G_AGCT'C Cuts at: 2028 3438 3804 4526 5457 7721 2028 Size: 1410 366 722 931 2264 5753 Fragments arranged by size: 5753 2264 1410 931 722 366 SalI G'TCGA_C Cuts at: 1936 7010 8788 1936 Size: 5074 1778 4594 Fragments arranged by size: 5074 4594 1778 SapI GCTCTTCn'nnn_ Cuts at: 2010 2151 4346 4998 5106 6711 7390 2010 Size: 141 2195 652 108 1605 679 6066 Fragments arranged by size: 6066 2195 1605 679 652 141 108 SbfI CC_TGCA'GG Cuts at: 3985 5259 7338 8798 3985 Size: 1274 2079 1460 6633 Fragments arranged by size: 6633 2079 1460 1274 ScaI AGT'ACT Cuts at: 2399 9694 11406 2399 Size: 7295 1712 2439 Fragments arranged by size: 7295 2439 1712 SexAI A'CCwGG_T Cuts at: 7059 7059 Size: 11446 SfcI C'TryA_G Cuts at: 826 1045 2411 2435 3190 3981 4553 4862 5187 Size: 219 1366 24 755 791 572 309 325 Cuts at: 5187 5255 5546 5984 6161 6185 6305 6371 6669 Size: 68 291 438 177 24 120 66 298 Cuts at: 6669 7037 7334 7491 8794 9929 10610 10801 826 Size: 368 297 157 1303 1135 681 191 1471 Fragments arranged by size: 1471 1366 1303 1135 791 755 681 572 438 368 325 309 298 297 291 219 191 177 157 120 68 66 24 24 SfiI GGCCn_nnn'nGGCC Cuts at: 5516 5516 Size: 11446 SfoI GGC'GCC Cuts at: 5158 5158 Size: 11446 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11446 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11446 SmaI CCC'GGG Cuts at: 1872 6260 8773 1872 Size: 4388 2513 4545 Fragments arranged by size: 4545 4388 2513 SmlI C'TyrA_G Cuts at: 820 1017 1943 2095 3138 3562 3667 3814 3825 Size: 197 926 152 1043 424 105 147 11 Cuts at: 3825 4898 6688 7912 9550 10451 10713 820 Size: 1073 1790 1224 1638 901 262 1553 Fragments arranged by size: 1790 1638 1553 1224 1073 1043 926 901 424 262 197 152 147 105 11 SnaBI TAC'GTA Cuts at: 493 9237 493 Size: 8744 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11446 SphI G_CATG'C Cuts at: 1132 3587 3874 4971 6397 7276 8804 1132 Size: 2455 287 1097 1426 879 1528 3774 Fragments arranged by size: 3774 2455 1528 1426 1097 879 287 SspI AAT'ATT Cuts at: 5 52 9370 5 Size: 47 9318 2081 Fragments arranged by size: 9318 2081 47 StuI AGG'CCT Cuts at: 1517 2441 3560 4250 5519 7445 1517 Size: 924 1119 690 1269 1926 5518 Fragments arranged by size: 5518 1926 1269 1119 924 690 StyI C'CwwG_G Cuts at: 513 1065 2004 2304 3000 3202 4687 5298 6663 Size: 552 939 300 696 202 1485 611 1365 Cuts at: 6663 6741 6802 7389 8684 8885 513 Size: 78 61 587 1295 201 3074 Fragments arranged by size: 3074 1485 1365 1295 939 696 611 587 552 300 202 201 78 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2864 3245 4334 5305 6397 9473 9490 Size: 1255 1284 381 1089 971 1092 3076 17 Cuts at: 9490 9643 9828 10273 325 Size: 153 185 445 1498 Fragments arranged by size: 3076 1498 1284 1255 1092 1089 971 445 381 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2319 2397 2655 Size: 274 80 33 51 1443 342 78 258 Cuts at: 2655 3937 6036 7228 7530 9692 11404 96 Size: 1282 2099 1192 302 2162 1712 138 Fragments arranged by size: 2162 2099 1712 1443 1282 1192 342 302 274 258 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4700 4732 8247 8279 11406 11438 4700 Size: 32 3515 32 3127 32 4708 Fragments arranged by size: 4708 3515 3127 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3949 6506 1316 Size: 2633 2557 6256 Fragments arranged by size: 6256 2633 2557 XbaI T'CTAG_A Cuts at: 6598 7450 8470 8782 6598 Size: 852 1020 312 9262 Fragments arranged by size: 9262 1020 852 312 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5380 5647 5380 Size: 267 11179 XhoI C'TCGA_G Cuts at: 1943 2095 3667 3825 1943 Size: 152 1572 158 9564 Fragments arranged by size: 9564 1572 158 152 XmaI C'CCGG_G Cuts at: 1870 6258 8771 1870 Size: 4388 2513 4545 Fragments arranged by size: 4545 4388 2513 XmnI GAAnn'nnTTC Cuts at: 2399 2751 7907 8148 9575 2399 Size: 352 5156 241 1427 4270 Fragments arranged by size: 5156 4270 1427 352 241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9254 276 Size: 53 83 186 936 7720 2468 Fragments arranged by size: 7720 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BsiWI BsmBI BstZ17I EcoRV FseI NruI PacI PmlI PsrI RsrII SacII SanDI SrfI SwaI