(Circular) (Six-base) MAPSORT of: pFN21AA0925.seq Check: 3117 from: 1 to: 6130 pFN21AA0925 6130 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2049 3473 1937 Size: 112 1424 4594 Fragments arranged by size: 4594 1424 112 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2375 4193 5577 5845 1632 Size: 743 1818 1384 268 1917 Fragments arranged by size: 1917 1818 1384 743 268 AfeI AGC'GCT Cuts at: 2455 2455 Size: 6130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 2316 5029 6006 1203 Size: 1113 2713 977 1327 Fragments arranged by size: 2713 1327 1113 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 2124 2321 2531 1166 Size: 958 197 210 4765 Fragments arranged by size: 4765 958 210 197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AlwNI CAG_nnn'CTG Cuts at: 3873 5445 3873 Size: 1572 4558 ApaLI G'TGCA_C Cuts at: 2108 4187 5343 2108 Size: 2079 1156 2895 Fragments arranged by size: 2895 2079 1156 ApoI r'AATT_y Cuts at: 1493 3439 3665 3817 5926 1493 Size: 1946 226 152 2109 1697 Fragments arranged by size: 2109 1946 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2342 3455 1870 Size: 73 399 1113 4545 Fragments arranged by size: 4545 1113 399 73 AvrII C'CTAG_G Cuts at: 2953 2953 Size: 6130 BamHI G'GATC_C Cuts at: 2088 3460 3827 2088 Size: 1372 367 4391 Fragments arranged by size: 4391 1372 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2424 3451 4906 618 Size: 325 217 627 637 1027 1455 1842 Fragments arranged by size: 1842 1455 1027 637 627 325 217 BanII G_rGCy'C Cuts at: 1391 2616 2799 3045 3303 3449 1391 Size: 1225 183 246 258 146 4072 Fragments arranged by size: 4072 1225 258 246 183 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3093 928 Size: 971 1194 3965 Fragments arranged by size: 3965 1194 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2250 4741 136 Size: 107 122 71 631 605 578 2491 1525 Fragments arranged by size: 2491 1525 631 605 578 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 2112 2397 3063 4191 5347 1790 Size: 322 285 666 1128 1156 2573 Fragments arranged by size: 2573 1156 1128 666 322 285 BmgBI CAC'GTC Cuts at: 1318 2319 1318 Size: 1001 5129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3861 4819 442 Size: 416 53 83 399 24 2444 958 1753 Fragments arranged by size: 2444 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2635 2779 2947 3064 2635 Size: 144 168 117 5701 Fragments arranged by size: 5701 168 144 117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2402 2701 3371 4790 1239 Size: 980 183 299 670 1419 2579 Fragments arranged by size: 2579 1419 980 670 299 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3011 4255 5120 5418 3011 Size: 1244 865 298 3723 Fragments arranged by size: 3723 1244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2719 4793 882 Size: 617 1220 2074 2219 Fragments arranged by size: 2219 2074 1220 617 BsaAI yAC'GTr Cuts at: 493 1204 2583 3921 493 Size: 711 1379 1338 2702 Fragments arranged by size: 2702 1379 1338 711 BsaBI GATnn'nnATC Cuts at: 2093 6124 2093 Size: 4031 2099 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2014 3937 4319 4954 Size: 53 83 186 936 481 1923 382 635 Cuts at: 4954 275 Size: 1451 Fragments arranged by size: 1923 1451 936 635 481 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2040 2874 4563 4961 5235 5382 5793 2040 Size: 834 1689 398 274 147 411 2377 Fragments arranged by size: 2377 1689 834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 2303 2333 1178 Size: 30 165 30 612 30 258 30 4975 Fragments arranged by size: 4975 612 258 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 3365 1186 Size: 1504 675 3951 Fragments arranged by size: 3951 1504 675 BseYI C'CCAG_C Cuts at: 2935 3142 5333 5829 2935 Size: 207 2191 496 3236 Fragments arranged by size: 3236 2191 496 207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 1163 Size: 573 636 4921 Fragments arranged by size: 4921 636 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 2112 2309 3303 4191 4276 5347 2076 Size: 36 197 994 888 85 1071 2859 Fragments arranged by size: 2859 1071 994 888 197 85 36 BsmI GAATG_Cn' Cuts at: 3375 3641 3734 3375 Size: 266 93 5771 Fragments arranged by size: 5771 266 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2112 2309 2397 2616 2799 3045 Size: 399 286 36 197 88 219 183 246 Cuts at: 3045 3063 3303 3449 4191 4276 5347 1391 Size: 18 240 146 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 286 246 240 219 197 183 146 88 85 36 18 BspEI T'CCGG_A Cuts at: 2040 5793 2040 Size: 3753 2377 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2619 2931 4017 1394 Size: 429 796 312 1086 3507 Fragments arranged by size: 3507 1086 796 429 312 BsrDI GCAATG_nn' Cuts at: 65 1248 2260 2295 4625 4799 65 Size: 1183 1012 35 2330 174 1396 Fragments arranged by size: 2330 1396 1183 1012 174 35 BsrFI r'CCGG_y Cuts at: 1700 1810 2400 2503 4774 4961 1700 Size: 110 590 103 2271 187 2869 Fragments arranged by size: 2869 2271 590 187 110 103 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 2302 4190 5202 2302 Size: 1888 1012 3230 Fragments arranged by size: 3230 1888 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 3307 4934 3307 Size: 1627 4503 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstYI r'GATC_y Cuts at: 1972 2088 2328 2337 3160 3460 3827 4212 4229 Size: 116 240 9 823 300 367 385 17 Cuts at: 4229 5681 5767 5779 6125 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 823 385 367 346 300 240 116 86 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5578 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2074 3646 4440 4460 2074 Size: 1572 794 20 3744 Fragments arranged by size: 3744 1572 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DraIII CAC_nnn'GTG Cuts at: 2583 2662 2583 Size: 79 6051 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2471 5137 809 Size: 507 1155 2666 1802 Fragments arranged by size: 2666 1802 1155 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2148 2242 Size: 54 1425 50 275 18 231 87 94 Cuts at: 2242 2623 3577 4466 6095 8 Size: 381 954 889 1629 43 Fragments arranged by size: 1629 1425 954 889 381 275 231 94 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2775 3233 4060 1353 Size: 1422 458 827 3423 Fragments arranged by size: 3423 1422 827 458 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3301 3301 Size: 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2375 2402 2701 3371 4193 4790 Size: 393 587 156 27 299 670 822 597 Cuts at: 4790 5577 5845 1239 Size: 787 268 1524 Fragments arranged by size: 1524 822 787 670 597 587 393 299 268 156 27 EcoO109I rG'GnC_Cy Cuts at: 2473 2758 3031 2473 Size: 285 273 5572 Fragments arranged by size: 5572 285 273 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 EcoRV GAT'ATC Cuts at: 2266 2266 Size: 6130 FspI TGC'GCA Cuts at: 3410 4636 3410 Size: 1226 4904 HaeII r_GCGC'y Cuts at: 1678 2378 2457 5277 1678 Size: 700 79 2820 2531 Fragments arranged by size: 2820 2531 700 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 2783 2815 4771 Size: 32 135 32 731 32 448 32 1956 Cuts at: 4771 4803 4845 4877 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1956 731 448 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 3253 3474 3720 669 Size: 988 281 196 1119 221 246 3079 Fragments arranged by size: 3079 1119 988 281 246 221 196 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5244 5428 1305 Size: 3939 184 2007 Fragments arranged by size: 3939 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2063 2150 10 Size: 54 1425 343 231 87 3990 Fragments arranged by size: 3990 1425 343 231 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2124 2321 2529 2531 2837 3050 3056 Size: 648 958 197 208 2 306 213 6 Cuts at: 3056 4088 4447 4606 518 Size: 1032 359 159 2042 Fragments arranged by size: 2042 1032 958 648 359 306 213 208 197 159 6 2 MspA1I CmG'CkG Cuts at: 1606 3391 3546 4223 5371 5616 1606 Size: 1785 155 677 1148 245 2120 Fragments arranged by size: 2120 1785 1148 677 245 155 NaeI GCC'GGC Cuts at: 2402 2402 Size: 6130 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5578 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NgoMIV G'CCGG_C Cuts at: 2400 2400 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2548 2842 3488 5033 1132 Size: 97 1319 294 646 1545 2229 Fragments arranged by size: 2229 1545 1319 646 294 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5228 PfoI T'CCnGG_A Cuts at: 2379 2379 Size: 6130 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PmlI CAC'GTG Cuts at: 2583 2583 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2473 2758 2473 Size: 285 5845 PshAI GACnn'nnGTC Cuts at: 1613 2711 1613 Size: 1098 5032 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 2637 3482 830 Size: 1807 845 3478 Fragments arranged by size: 3478 1807 845 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 3391 3391 Size: 6130 SacI G_AGCT'C Cuts at: 3303 3303 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2633 3478 4613 5294 5485 826 Size: 219 1588 845 1135 681 191 1471 Fragments arranged by size: 1588 1471 1135 845 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3026 4234 5135 5397 820 Size: 197 926 1083 1208 901 262 1553 Fragments arranged by size: 1553 1208 1083 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 2952 1517 Size: 1435 4695 StyI C'CwwG_G Cuts at: 513 1065 2232 2953 3569 513 Size: 552 1167 721 616 3074 Fragments arranged by size: 3074 1167 721 616 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2486 2849 3298 4157 4174 4327 4512 Size: 1255 906 363 449 859 17 153 185 Cuts at: 4512 4957 325 Size: 445 1498 Fragments arranged by size: 1498 1255 906 859 449 445 363 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4376 6088 96 Size: 274 80 33 51 1443 2399 1712 138 Fragments arranged by size: 2399 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6090 6122 6090 Size: 32 6098 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2974 2974 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 2279 4259 2279 Size: 1980 4150 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI