(Circular) (Six-base) MAPSORT of: pFN21AA0878.seq Check: 6435 from: 1 to: 6538 pFN21AA0878 6538 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4348 278 Size: 53 83 186 936 2812 2468 Fragments arranged by size: 2812 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3881 1937 Size: 1944 4594 Acc65I G'GTAC_C Cuts at: 2405 3859 2405 Size: 1454 5084 AclI AA'CG_TT Cuts at: 1563 2307 4665 5038 1563 Size: 744 2358 373 3063 Fragments arranged by size: 3063 2358 744 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 2730 2952 3676 4601 5985 6253 1632 Size: 870 228 222 724 925 1384 268 1917 Fragments arranged by size: 1917 1384 925 870 724 268 228 222 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6341 AflIII A'CryG_T Cuts at: 1203 5437 6414 1203 Size: 4234 977 1327 Fragments arranged by size: 4234 1327 977 AgeI A'CCGG_T Cuts at: 5369 5369 Size: 6538 AhdI GACnn_n'nnGTC Cuts at: 5267 5267 Size: 6538 AleI CACnn'nnGTG Cuts at: 1166 3531 1166 Size: 2365 4173 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2147 1184 Size: 963 5575 AlwNI CAG_nnn'CTG Cuts at: 4281 5853 4281 Size: 1572 4966 ApaLI G'TGCA_C Cuts at: 4595 5751 4595 Size: 1156 5382 ApoI r'AATT_y Cuts at: 1493 2374 3279 3707 3847 4073 4225 6334 1493 Size: 881 905 428 140 226 152 2109 1697 Fragments arranged by size: 2109 1697 905 881 428 226 152 140 AseI AT'TA_AT Cuts at: 160 1032 3168 5092 160 Size: 872 2136 1924 1606 Fragments arranged by size: 2136 1924 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 AvaI C'yCGr_G Cuts at: 1870 1943 3863 1870 Size: 73 1920 4545 Fragments arranged by size: 4545 1920 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3279 3312 3279 Size: 33 6505 BamHI G'GATC_C Cuts at: 2487 3868 4235 2487 Size: 1381 367 4790 Fragments arranged by size: 4790 1381 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2405 3859 5314 618 Size: 325 217 627 618 1454 1455 1842 Fragments arranged by size: 1842 1455 1454 627 618 325 217 BanII G_rGCy'C Cuts at: 1391 3857 1391 Size: 2466 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2615 3265 928 Size: 971 716 650 4201 Fragments arranged by size: 4201 971 716 650 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4729 4763 1903 Size: 34 2792 34 3678 Fragments arranged by size: 3678 2792 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4430 5640 4430 Size: 1210 5328 BclI T'GATC_A Cuts at: 1548 2295 1548 Size: 747 5791 BfrBI ATG'CAT Cuts at: 3144 3144 Size: 6538 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2071 3308 3462 Size: 107 122 71 631 605 399 1237 154 Cuts at: 3462 5149 136 Size: 1687 1525 Fragments arranged by size: 1687 1525 1237 631 605 399 154 122 107 71 BglII A'GATC_T Cuts at: 6533 6533 Size: 6538 BlpI GC'TnA_GC Cuts at: 3955 3955 Size: 6538 Bme1580I G_kGCm'C Cuts at: 1790 4599 5755 1790 Size: 2809 1156 2573 Fragments arranged by size: 2809 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2770 1318 Size: 1452 5086 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4269 5227 442 Size: 416 53 83 399 24 2852 958 1753 Fragments arranged by size: 2852 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2958 1056 Size: 1902 4636 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2118 2118 Size: 6538 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5198 1239 Size: 3959 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3218 4663 5528 5826 3218 Size: 1445 865 298 3930 Fragments arranged by size: 3930 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2113 5201 882 Size: 617 614 3088 2219 Fragments arranged by size: 3088 2219 617 614 BsaAI yAC'GTr Cuts at: 493 1204 4329 493 Size: 711 3125 2702 Fragments arranged by size: 3125 2702 711 BsaBI GATnn'nnATC Cuts at: 6532 6532 Size: 6538 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3003 4345 4727 5362 Size: 53 83 186 936 1470 1342 382 635 Cuts at: 5362 275 Size: 1451 Fragments arranged by size: 1470 1451 1342 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2161 4971 5369 5643 5790 6201 2161 Size: 2810 398 274 147 411 2498 Fragments arranged by size: 2810 2498 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3431 3461 1178 Size: 30 165 30 2028 30 4255 Fragments arranged by size: 4255 2028 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2583 1186 Size: 1397 5141 BseYI C'CCAG_C Cuts at: 2026 3457 3552 5741 6237 2026 Size: 1431 95 2189 496 2327 Fragments arranged by size: 2327 2189 1431 496 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5965 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2066 2154 3988 4749 5777 1540 Size: 275 186 65 88 1834 761 1028 2301 Fragments arranged by size: 2301 1834 1028 761 275 186 88 65 BsiHKAI G_wGCw'C Cuts at: 4599 4684 5755 4599 Size: 85 1071 5382 Fragments arranged by size: 5382 1071 85 BsmI GAATG_Cn' Cuts at: 4049 4142 4049 Size: 93 6445 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3857 4599 4684 5755 1391 Size: 399 2067 742 85 1071 2174 Fragments arranged by size: 2174 2067 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6201 6201 Size: 6538 BspHI T'CATG_A Cuts at: 4427 6157 4427 Size: 1730 4808 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3618 3893 844 Size: 2774 275 3489 Fragments arranged by size: 3489 2774 275 BsrBI CCG'CTC Cuts at: 1394 1823 4425 1394 Size: 429 2602 3507 Fragments arranged by size: 3507 2602 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3669 5033 5207 65 Size: 1183 2421 1364 174 1396 Fragments arranged by size: 2421 1396 1364 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2061 5182 5369 1700 Size: 110 251 3121 187 2869 Fragments arranged by size: 3121 2869 251 187 110 BsrGI T'GTAC_A Cuts at: 96 2432 3438 96 Size: 2336 1006 3196 Fragments arranged by size: 3196 2336 1006 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6538 BssSI C'ACGA_G Cuts at: 4598 5610 4598 Size: 1012 5526 BstAPI GCAn_nnn'nTGC Cuts at: 794 3565 794 Size: 2771 3767 BstBI TT'CG_AA Cuts at: 3276 5342 3276 Size: 2066 4472 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6538 BstXI CCAn_nnnn'nTGG Cuts at: 3319 3319 Size: 6538 BstYI r'GATC_y Cuts at: 1972 2487 2914 3715 3868 4235 4620 4637 6089 Size: 515 427 801 153 367 385 17 1452 Cuts at: 6089 6175 6187 6533 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 801 515 427 385 367 346 153 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3176 3176 Size: 6538 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5986 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5799 Fragments arranged by size: 5799 683 56 BtsI GCAGTG_nn' Cuts at: 4054 4848 4868 4054 Size: 794 20 5724 Fragments arranged by size: 5724 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6538 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3623 3658 529 Size: 35 3059 35 3409 Fragments arranged by size: 3409 3059 35 35 DraI TTT'AAA Cuts at: 2793 3150 3842 2793 Size: 357 692 5489 Fragments arranged by size: 5489 692 357 DraIII CAC_nnn'GTG Cuts at: 2020 2020 Size: 6538 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2225 3418 5545 809 Size: 507 909 1193 2127 1802 Fragments arranged by size: 2127 1802 1193 909 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2063 2142 3758 Size: 54 1425 50 275 18 233 79 1616 Cuts at: 3758 3985 4874 6503 8 Size: 227 889 1629 43 Fragments arranged by size: 1629 1616 1425 889 275 233 227 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2063 3985 1537 Size: 275 251 1922 4090 Fragments arranged by size: 4090 1922 275 251 EarI CTCTTCn'nnn_ Cuts at: 1353 2415 2861 3014 4468 1353 Size: 1062 446 153 1454 3423 Fragments arranged by size: 3423 1454 1062 446 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5125 5499 5645 387 Size: 1064 138 3536 374 146 1280 Fragments arranged by size: 3536 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2502 2730 2952 3676 4601 5198 5985 Size: 393 870 228 222 724 925 597 787 Cuts at: 5985 6253 1239 Size: 268 1524 Fragments arranged by size: 1524 925 870 787 724 597 393 268 228 222 EcoRI G'AATT_C Cuts at: 3847 4225 3847 Size: 378 6160 EcoRV GAT'ATC Cuts at: 3085 3085 Size: 6538 FspI TGC'GCA Cuts at: 5044 5044 Size: 6538 HaeII r_GCGC'y Cuts at: 1678 2027 2283 5685 1678 Size: 349 256 3402 2531 Fragments arranged by size: 3402 2531 349 256 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5179 5211 5253 5285 1373 Size: 32 135 32 3607 32 42 32 2626 Fragments arranged by size: 3607 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3607 3882 4128 669 Size: 988 281 1669 275 246 3079 Fragments arranged by size: 3079 1669 988 281 275 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6538 HpaI GTT'AAC Cuts at: 4128 4128 Size: 6538 KpnI G_GTAC'C Cuts at: 2409 3863 2409 Size: 1454 5084 MfeI C'AATT_G Cuts at: 4137 4137 Size: 6538 MluI A'CGCG_T Cuts at: 6414 6414 Size: 6538 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2515 2556 5652 5836 1305 Size: 1210 41 3096 184 2007 Fragments arranged by size: 3096 2007 1210 184 41 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4716 Fragments arranged by size: 4716 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 3317 3531 4496 4855 5014 518 Size: 648 1125 1026 214 965 359 159 2042 Fragments arranged by size: 2042 1125 1026 965 648 359 214 159 MspA1I CmG'CkG Cuts at: 1606 3461 3556 3954 4631 5779 6024 1606 Size: 1855 95 398 677 1148 245 2120 Fragments arranged by size: 2120 1855 1148 677 398 245 95 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5986 NdeI CA'TA_TG Cuts at: 387 387 Size: 6538 NheI G'CTAG_C Cuts at: 1052 2954 1052 Size: 1902 4636 NotI GC'GGCC_GC Cuts at: 3985 3985 Size: 6538 NsiI A_TGCA'T Cuts at: 3146 3146 Size: 6538 NspI r_CATG'y Cuts at: 1132 1229 2439 3568 3896 5441 1132 Size: 97 1210 1129 328 1545 2229 Fragments arranged by size: 2229 1545 1210 1129 328 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6538 PciI A'CATG_T Cuts at: 5437 5437 Size: 6538 PflMI CCAn_nnn'nTGG Cuts at: 1108 2020 2764 1108 Size: 912 744 4882 Fragments arranged by size: 4882 912 744 PfoI T'CCnGG_A Cuts at: 3812 3812 Size: 6538 PmeI GTTT'AAAC Cuts at: 3842 3842 Size: 6538 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4607 4639 6120 6152 4607 Size: 32 1481 32 4993 Fragments arranged by size: 4993 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6538 PsiI TTA'TAA Cuts at: 2724 2781 3222 4108 2724 Size: 57 441 886 5154 Fragments arranged by size: 5154 886 441 57 PstI C_TGCA'G Cuts at: 830 3525 3561 3890 830 Size: 2695 36 329 3478 Fragments arranged by size: 3478 2695 329 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6538 PvuII CAG'CTG Cuts at: 3461 3556 3461 Size: 95 6443 SalI G'TCGA_C Cuts at: 1936 3880 1936 Size: 1944 4594 SbfI CC_TGCA'GG Cuts at: 3890 3890 Size: 6538 ScaI AGT'ACT Cuts at: 4786 6498 4786 Size: 1712 4826 SfcI C'TryA_G Cuts at: 826 1045 3521 3557 3886 5021 5702 5893 826 Size: 219 2476 36 329 1135 681 191 1471 Fragments arranged by size: 2476 1471 1135 681 329 219 191 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6538 SmaI CCC'GGG Cuts at: 1872 3865 1872 Size: 1993 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3233 4642 5543 5805 820 Size: 197 926 1290 1409 901 262 1553 Fragments arranged by size: 1553 1409 1290 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4329 493 Size: 3836 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6538 SphI G_CATG'C Cuts at: 1132 3568 3896 1132 Size: 2436 328 3774 Fragments arranged by size: 3774 2436 328 SspI AAT'ATT Cuts at: 5 52 4462 5 Size: 47 4410 2081 Fragments arranged by size: 4410 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6538 StyI C'CwwG_G Cuts at: 513 1065 3977 513 Size: 552 2912 3074 Fragments arranged by size: 3074 2912 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4565 4582 4735 4920 5365 325 Size: 1255 2985 17 153 185 445 1498 Fragments arranged by size: 2985 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2432 2474 3438 Size: 274 80 33 51 1443 455 42 964 Cuts at: 3438 3771 4784 6496 96 Size: 333 1013 1712 138 Fragments arranged by size: 1712 1443 1013 964 455 333 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6498 6530 6498 Size: 32 6506 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6538 XbaI T'CTAG_A Cuts at: 3874 3874 Size: 6538 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2017 Size: 6538 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6538 XmaI C'CCGG_G Cuts at: 1870 3863 1870 Size: 1993 4545 XmnI GAAnn'nnTTC Cuts at: 4667 4667 Size: 6538 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4346 276 Size: 53 83 186 936 2812 2468 Fragments arranged by size: 2812 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI