Restriction map for
pFN21AA0830
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AclI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI Asp700I AspEI AviII AvrII
BciVI BclI BfrI BlnI BsaBI
BsaI BsgI BsiWI BsmBI BspHI
BspLU11I BsrBI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CpoI DraI DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI KspI MamI
MluI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 1414 GGTACC G^GTACC
AccI 936 GTCTAC GT^[AC][GT]AC
AccIII 1238 TCCGGA T^CCGGA
AcsI 483 AAATTC [GA]^AATT[CT]
AcyI 268 GGCGCC G[GA]^CG[CT]C
Aor51HI 188 AGCGCT AGC^GCT
ApoI 483 AAATTC [GA]^AATT[CT]
Asp718I 1414 GGTACC G^GTACC
AspHI 1201 GTGCTC G[AT]GC[AT]^C
AspI 498 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1241 GGATCC G^GATCC
BbeI 268 GGCGCC GGCGC^C
BbrPI 154 CACGTG CAC^GTG
BbsI 580 GAAGAC GAAGAC(2/6)
BbvCI 784 CCTCAGC CCTCAGC(-5/-2)
BcgI 29 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 580 GAAGAC GAAGAC(2/6)
BsaAI 154 CACGTG [CT]AC^GT[GA]
BsaHI 268 GGCGCC G[GA]^CG[CT]C
BsaWI 1238 TCCGGA [AT]^CCGG[AT]
BseAI 1238 TCCGGA T^CCGGA
BsiEI 143 CGGCCG CG[GA][CT]^CG
BsiHKAI 1201 GTGCTC G[AT]GC[AT]^C
BsmAI 1387 GTCTC GTCTC(1/5)
BsmI 1187 GCATTC GAATGC(1/-1)
Bsp1407I 1325 TGTACA T^GTACA
BspDI 301 ATCGAT AT^CGAT
BspEI 1238 TCCGGA T^CCGGA
BspMI 1098 GCAGGT ACCTGC(4/8)
BsrGI 1325 TGTACA T^GTACA
BsrI 1368 ACTGG ACTGG(1/-1)
BssSI 67 CTCGTG CACGAG(-5/-1)
ClaI 301 ATCGAT AT^CGAT
DraII 121 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 563 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 143 CGGCCG C^GGCCG
EclXI 143 CGGCCG C^GGCCG
Eco47III 188 AGCGCT AGC^GCT
Eco52I 143 CGGCCG C^GGCCG
EcoO109I 121 GGGACCC [GA]G^G[ACGT]CC[CT]
Hin1I 268 GGCGCC G[GA]^CG[CT]C
HincII 856 GTTAAC GT[CT]^[GA]AC
HindII 856 GTTAAC GT[CT]^[GA]AC
HindIII 1021 AAGCTT A^AGCTT
HpaI 856 GTTAAC GTT^AAC
KasI 268 GGCGCC G^GCGCC
KpnI 1414 GGTACC GGTAC^C
MaeII 155 ACGT A^CGT
MfeI 1346 CAATTG C^AATTG
MroI 1238 TCCGGA T^CCGGA
MspA1I 787 CAGCTG C[AC]G^C[GT]G
MunI 1346 CAATTG C^AATTG
NarI 268 GGCGCC GG^CGCC
PflFI 498 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 562 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 498 GACTC GAGTC(4/5)
PmaCI 154 CACGTG CAC^GTG
PmlI 154 CACGTG CAC^GTG
PpuMI 121 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 787 CAGCTG CAG^CTG
SfoI 268 GGCGCC GGC^GCC
SspBI 1325 TGTACA T^GTACA
TaqI 302 TCGA T^CGA
Tth111I 498 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 302 TCGA T^CGA
Van91I 562 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AlwI 1242 GATCC GGATC(4/5)
1241 GGATC
AlwNI 641 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
763 CAGAAACTG
AvaI 212 CCCGGG C^[CT]CG[GA]G
719 CCCGGG
BanII 1137 GGGCTC G[GA]GC[CT]^C
1229 GGGCTC
BfaI 905 CTAG C^TAG
917 CTAG
BglI 129 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1373 GCCTTGGTGGC
BglII 161 AGATCT A^GATCT
756 AGATCT
BlpI 184 GCTGAGC GC^T[ACGT]AGC
1139 GCTCAGC
BpmI 950 CTCCAG CTGGAG(16/14)
683 CTGGAG
Bpu1102I 184 GCTGAGC GC^T[ACGT]AGC
1139 GCTCAGC
BsoBI 212 CCCGGG C^[CT]CG[GA]G
719 CCCGGG
BsrDI 1002 CATTGC GCAATG(2/0)
1362 CATTGC
BsrFI 62 GCCGGC [GA]^CCGG[CT]
85 GCCGGC
BssHII 10 GCGCGC G^CGCGC
171 GCGCGC
CelII 184 GCTGAGC GC^T[ACGT]AGC
1139 GCTCAGC
Cfr10I 62 GCCGGC [GA]^CCGG[CT]
85 GCCGGC
DsaI 698 CCATGG C^C[GA][CT]GG
1331 CCATGG
EarI 34 CTCTTC CTCTTC(1/4)
1174 GAAGAG
Ksp632I 34 CTCTTC CTCTTC(1/4)
1174 GAAGAG
MaeI 905 CTAG C^TAG
917 CTAG
MslI 222 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
354 CACCTCTGTG
NaeI 62 GCCGGC GCC^GGC
85 GCCGGC
NcoI 698 CCATGG C^CATGG
1331 CCATGG
NgoMIV 62 GCCGGC G^CCGGC
85 GCCGGC
SfcI 204 CTACAG C^T[GA][CT]AG
618 CTACAG
SmaI 212 CCCGGG CCC^GGG
719 CCCGGG
SmlI 322 CTTGAG C^T[CT][GA]AG
1204 CTCAAG
Tsp45I 101 GTGAC ^GT[GC]AC
728 GTGAC
XmaCI 212 CCCGGG C^CCGGG
719 CCCGGG
XmaI 212 CCCGGG C^CCGGG
719 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AseI
AsnI AvaII BalI BanI BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII