(Circular) (Six-base) MAPSORT of: pFN21AA0830.seq Check: 7087 from: 1 to: 6205 pFN21AA0830 6205 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3094 3094 Size: 6205 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2942 3548 1937 Size: 1005 606 4594 Fragments arranged by size: 4594 1005 606 Acc65I G'GTAC_C Cuts at: 3419 3526 3419 Size: 107 6098 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2750 3086 3284 4268 5652 5920 1632 Size: 1118 336 198 984 1384 268 1917 Fragments arranged by size: 1917 1384 1118 984 336 268 198 AfeI AGC'GCT Cuts at: 2195 2195 Size: 6205 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 2363 1166 Size: 1197 5008 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AlwNI CAG_nnn'CTG Cuts at: 2651 2773 3948 5520 2651 Size: 122 1175 1572 3336 Fragments arranged by size: 3336 1572 1175 122 ApaLI G'TGCA_C Cuts at: 4262 5418 4262 Size: 1156 5049 ApoI r'AATT_y Cuts at: 1493 2488 3514 3740 3892 6001 1493 Size: 995 1026 226 152 2109 1697 Fragments arranged by size: 2109 1697 1026 995 226 152 AseI AT'TA_AT Cuts at: 160 1032 2340 3038 3111 4759 160 Size: 872 1308 698 73 1648 1606 Fragments arranged by size: 1648 1606 1308 872 698 73 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2217 2724 3530 1870 Size: 73 274 507 806 4545 Fragments arranged by size: 4545 806 507 274 73 BamHI G'GATC_C Cuts at: 3246 3535 3902 3246 Size: 289 367 5549 Fragments arranged by size: 5549 367 289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2273 3419 3526 4981 Size: 325 217 627 222 264 1146 107 1455 Cuts at: 4981 618 Size: 1842 Fragments arranged by size: 1842 1455 1146 627 325 264 222 217 107 BanII G_rGCy'C Cuts at: 1391 3146 3238 3524 1391 Size: 1755 92 286 4072 Fragments arranged by size: 4072 1755 286 92 BbeI G_GCGC'C Cuts at: 2277 2277 Size: 6205 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2592 928 Size: 971 693 4541 Fragments arranged by size: 4541 971 693 BbvCI CC'TCA_GC Cuts at: 2790 2790 Size: 6205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2023 2057 4396 4430 1903 Size: 34 86 34 2339 34 3678 Fragments arranged by size: 3678 2339 86 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2140 3384 4816 Size: 107 122 71 631 605 468 1244 1432 Cuts at: 4816 136 Size: 1525 Fragments arranged by size: 1525 1432 1244 631 605 468 122 107 71 BglII A'GATC_T Cuts at: 2166 2761 6200 2166 Size: 595 3439 2171 Fragments arranged by size: 3439 2171 595 BlpI GC'TnA_GC Cuts at: 2190 3145 3622 2190 Size: 955 477 4773 Fragments arranged by size: 4773 955 477 Bme1580I G_kGCm'C Cuts at: 1790 2012 2616 4266 5422 1790 Size: 222 604 1650 1156 2573 Fragments arranged by size: 2573 1650 1156 604 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3383 3936 4894 Size: 416 53 83 399 24 1966 553 958 Cuts at: 4894 442 Size: 1753 Fragments arranged by size: 1966 1753 958 553 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2961 2961 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2709 2940 4865 1239 Size: 1470 231 1925 2579 Fragments arranged by size: 2579 1925 1470 231 Bpu10I CC'TnA_GC Cuts at: 2450 2790 2450 Size: 340 5865 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2348 3194 4330 5195 5493 2348 Size: 846 1136 865 298 3060 Fragments arranged by size: 3060 1136 865 846 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4868 882 Size: 617 3369 2219 Fragments arranged by size: 3369 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2161 3996 493 Size: 711 957 1835 2702 Fragments arranged by size: 2702 1835 957 711 BsaBI GATnn'nnATC Cuts at: 6199 6199 Size: 6205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2274 4012 4394 5029 Size: 53 83 186 936 741 1738 382 635 Cuts at: 5029 275 Size: 1451 Fragments arranged by size: 1738 1451 936 741 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3243 4638 5036 5310 5457 5868 3243 Size: 1395 398 274 147 411 3580 Fragments arranged by size: 3580 1395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2429 2459 1178 Size: 30 165 30 1026 30 4924 Fragments arranged by size: 4924 1026 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2345 2706 3230 1186 Size: 910 249 361 524 4161 Fragments arranged by size: 4161 910 524 361 249 BseYI C'CCAG_C Cuts at: 2029 2051 2697 5408 5904 2029 Size: 22 646 2711 496 2330 Fragments arranged by size: 2711 2330 646 496 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5632 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2151 3655 4416 5444 1540 Size: 275 186 150 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 186 150 BsiHKAI G_wGCw'C Cuts at: 3210 4266 4351 5422 3210 Size: 1056 85 1071 3993 Fragments arranged by size: 3993 1071 1056 85 BsmI GAATG_Cn' Cuts at: 3192 3716 3809 3192 Size: 524 93 5588 Fragments arranged by size: 5588 524 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2616 3146 3210 3238 3524 4266 Size: 399 222 604 530 64 28 286 742 Cuts at: 4266 4351 5422 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 530 399 286 222 85 64 28 BspEI T'CCGG_A Cuts at: 3243 5868 3243 Size: 2625 3580 BspHI T'CATG_A Cuts at: 4094 5824 4094 Size: 1730 4475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3094 3560 844 Size: 2250 466 3489 Fragments arranged by size: 3489 2250 466 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3006 3366 4700 4874 65 Size: 1183 1758 360 1334 174 1396 Fragments arranged by size: 1758 1396 1334 1183 360 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 2090 4849 5036 1700 Size: 110 257 23 2759 187 2869 Fragments arranged by size: 2869 2759 257 187 110 23 BsrGI T'GTAC_A Cuts at: 96 3330 96 Size: 3234 2971 BssHII G'CGCG_C Cuts at: 1925 2015 2176 1925 Size: 90 161 5954 Fragments arranged by size: 5954 161 90 BssSI C'ACGA_G Cuts at: 2072 4265 5277 2072 Size: 2193 1012 3000 Fragments arranged by size: 3000 2193 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6205 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstYI r'GATC_y Cuts at: 1972 2166 2761 3246 3535 3902 4287 4304 5756 Size: 194 595 485 289 367 385 17 1452 Cuts at: 5756 5842 5854 6200 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 595 485 385 367 346 289 194 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2703 3336 513 Size: 552 939 699 633 3382 Fragments arranged by size: 3382 939 699 633 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2299 487 Size: 683 56 1073 4393 Fragments arranged by size: 4393 1073 683 56 BtsI GCAGTG_nn' Cuts at: 2461 2584 3395 3408 3721 4515 4535 2461 Size: 123 811 13 313 794 20 4131 Fragments arranged by size: 4131 811 794 313 123 20 13 ClaI AT'CG_AT Cuts at: 1554 2307 1554 Size: 753 5452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DraIII CAC_nnn'GTG Cuts at: 2573 2573 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2469 3168 Size: 54 1425 50 275 18 318 321 699 Cuts at: 3168 3219 3315 3652 4541 6170 8 Size: 51 96 337 889 1629 43 Fragments arranged by size: 1629 1425 889 699 337 321 318 275 96 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2148 3652 1537 Size: 275 336 1504 4090 Fragments arranged by size: 4090 1504 336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 3174 4135 1353 Size: 692 1129 961 3423 Fragments arranged by size: 3423 1129 961 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2709 2750 2940 3086 3284 4268 4865 Size: 393 1077 41 190 146 198 984 597 Cuts at: 4865 5652 5920 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1077 984 787 597 393 268 198 190 146 41 EcoNI CCTnn'n_nnAGG Cuts at: 2330 2691 3437 2330 Size: 361 746 5098 Fragments arranged by size: 5098 746 361 EcoO109I rG'GnC_Cy Cuts at: 2127 2127 Size: 6205 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2407 2407 Size: 6205 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 2197 2277 3345 5352 1678 Size: 519 80 1068 2007 2531 Fragments arranged by size: 2531 2007 1068 519 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2896 2928 4846 4878 4920 Size: 32 135 32 1324 32 1918 32 42 Cuts at: 4920 4952 1373 Size: 32 2626 Fragments arranged by size: 2626 1918 1324 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2863 3549 3795 669 Size: 988 281 925 686 246 3079 Fragments arranged by size: 3079 988 925 686 281 246 HindIII A'AGCT_T Cuts at: 748 3026 748 Size: 2278 3927 HpaI GTT'AAC Cuts at: 2863 3795 2863 Size: 932 5273 KasI G'GCGC_C Cuts at: 2273 2273 Size: 6205 KpnI G_GTAC'C Cuts at: 3423 3530 3423 Size: 107 6098 MfeI C'AATT_G Cuts at: 3351 3804 3351 Size: 453 5752 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5319 5503 1305 Size: 4014 184 2007 Fragments arranged by size: 4014 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2471 3170 3221 3317 10 Size: 54 1425 343 639 699 51 96 2898 Fragments arranged by size: 2898 1425 699 639 343 96 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2363 4163 4522 4681 518 Size: 648 1065 132 1800 359 159 2042 Fragments arranged by size: 2042 1800 1065 648 359 159 132 MspA1I CmG'CkG Cuts at: 1606 2794 3621 4298 5446 5691 1606 Size: 1188 827 677 1148 245 2120 Fragments arranged by size: 2120 1188 1148 827 677 245 NaeI GCC'GGC Cuts at: 2069 2092 2069 Size: 23 6182 NarI GG'CG_CC Cuts at: 2274 2274 Size: 6205 NcoI C'CATG_G Cuts at: 513 1065 2004 2703 3336 513 Size: 552 939 699 633 3382 Fragments arranged by size: 3382 939 699 633 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NgoMIV G'CCGG_C Cuts at: 2067 2090 2067 Size: 23 6182 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 3563 5108 1132 Size: 97 2334 1545 2229 Fragments arranged by size: 2334 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3424 1348 Size: 2076 4129 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 2573 1108 Size: 1465 4740 PfoI T'CCnGG_A Cuts at: 2514 2850 2871 2514 Size: 336 21 5848 Fragments arranged by size: 5848 336 21 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PmlI CAC'GTG Cuts at: 2161 2161 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2127 2127 Size: 6205 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2228 2260 2874 2906 2228 Size: 32 614 32 5527 Fragments arranged by size: 5527 614 32 32 PstI C_TGCA'G Cuts at: 830 3557 830 Size: 2727 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 PvuII CAG'CTG Cuts at: 2794 2794 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SanDI GG'GwC_CC Cuts at: 2127 2127 Size: 6205 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 2209 2623 3553 4688 5369 5560 826 Size: 219 1164 414 930 1135 681 191 1471 Fragments arranged by size: 1471 1164 1135 930 681 414 219 191 SfoI GGC'GCC Cuts at: 2275 2275 Size: 6205 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 2219 2726 3532 1872 Size: 347 507 806 4545 Fragments arranged by size: 4545 806 507 347 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 3209 4309 5210 5472 820 Size: 197 926 384 882 1100 901 262 1553 Fragments arranged by size: 1553 1100 926 901 882 384 262 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 2004 2703 3336 3379 3644 513 Size: 552 939 699 633 43 265 3074 Fragments arranged by size: 3074 939 699 633 552 265 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3416 4232 4249 4402 4587 5032 325 Size: 1255 1836 816 17 153 185 445 1498 Fragments arranged by size: 1836 1498 1255 816 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 3330 4451 Size: 274 80 33 51 1443 258 1095 1121 Cuts at: 4451 6163 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1121 1095 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6165 6197 6165 Size: 32 6173 Tth111I GACn'n_nGTC Cuts at: 1316 2506 1316 Size: 1190 5015 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 2217 2724 3530 1870 Size: 347 507 806 4545 Fragments arranged by size: 4545 806 507 347 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI