Restriction map for
pFN21AA0820
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbvCI BcgI BfrI BlnI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII Bsu36I ClaI
CpoI DraI Eam1105I Eco47III Eco81I
EcoRI EcoT22I FseI FspI Hin1I
HpaI KasI KpnI KspI MamI
MluNI MroI MscI NarI NdeI
NotI NruI NsiI PacI PflFI
PflMI PinAI PmeI PshAI PvuI
RsrII SacII SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AclI 2044 AACGTT AA^CGTT
ApaI 2489 GGGCCC GGGCC^C
BanI 2229 GGCACC G^G[CT][GA]CC
BbrPI 382 CACGTG CAC^GTG
BclI 2036 TGATCA T^GATCA
BglI 751 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2004 AGATCT A^GATCT
BsiEI 2343 CGGCCG CG[GA][CT]^CG
BspLU11I 1976 ACATGT A^CATGT
BsrFI 124 GCCGGC [GA]^CCGG[CT]
BssSI 492 CACGAG CACGAG(-5/-1)
Bst1107I 1774 GTATAC GTA^TAC
BstAPI 232 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2156 TTCGAA TT^CGAA
BstEII 2273 GGTCACC G^GT[ACGT]ACC
BstPI 2273 GGTCACC G^GT[ACGT]ACC
BstXI 1328 CCAAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1774 GTATAC GTA^TAC
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
DraIII 2312 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1438 GACATTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2343 CGGCCG C^GGCCG
EclXI 2343 CGGCCG C^GGCCG
Eco52I 2343 CGGCCG C^GGCCG
EcoO65I 2273 GGTCACC G^GT[ACGT]ACC
EcoRV 451 GATATC GAT^ATC
FbaI 2036 TGATCA T^GATCA
HgaI 52 GACGC GACGC(5/10)
HincII 1825 GTCGAC GT[CT]^[GA]AC
HindII 1825 GTCGAC GT[CT]^[GA]AC
HindIII 1632 AAGCTT A^AGCTT
MfeI 1930 CAATTG C^AATTG
MluI 53 ACGCGT A^CGCGT
MslI 1387 CACATTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1930 CAATTG C^AATTG
NaeI 124 GCCGGC GCC^GGC
NcoI 1919 CCATGG C^CATGG
NgoMIV 124 GCCGGC G^CCGGC
NheI 564 GCTAGC G^CTAGC
NspV 2156 TTCGAA TT^CGAA
PmaCI 382 CACGTG CAC^GTG
PmlI 382 CACGTG CAC^GTG
PpuMI 836 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 2044 AACGTT AA^CGTT
PvuII 1830 CAGCTG CAG^CTG
SalI 1825 GTCGAC G^TCGAC
SexAI 1418 ACCAGGT A^CC[AT]GGT
SfuI 2156 TTCGAA TT^CGAA
StuI 2479 AGGCCT AGG^CCT
XbaI 657 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 54 CGCG CG^CG
319 CGCG
AfaI 1325 GTAC GT^AC
1674 GTAC
AlwNI 1350 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
2354 CAGCTCCTG
AseI 358 ATTAAT AT^TAAT
1302 ATTAAT
AsnI 358 ATTAAT AT^TAAT
1302 ATTAAT
Asp700I 768 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1102 GAACGCTTTC
AvaI 142 CTCGAG C^[CT]CG[GA]G
173 CTCGAG
BsaAI 382 CACGTG [CT]AC^GT[GA]
691 TACGTG
BsaI 1948 GAGACC GGTCTC(1/5)
2514 GAGACC
BsoBI 142 CTCGAG C^[CT]CG[GA]G
173 CTCGAG
BspHI 1098 TCATGA T^CATGA
1586 TCATGA
BspMI 827 ACCTGC ACCTGC(4/8)
2270 GCAGGT
BsrDI 253 CATTGC GCAATG(2/0)
983 CATTGC
BstUI 54 CGCG CG^CG
319 CGCG
BstYI 1284 GGATCT [GA]^GATC[CT]
2004 AGATCT
DsaI 104 CCGTGG C^C[GA][CT]GG
1919 CCATGG
EcoNI 828 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2109 CCTGATGGAGG
FauI 30 CCCGC CCCGC(4/6)
2493 CCCGC
MflI 1284 GGATCT [GA]^GATC[CT]
2004 AGATCT
MspA1I 31 CCGCTG C[AC]G^C[GT]G
1830 CAGCTG
MvnI 54 CGCG CG^CG
319 CGCG
PaeR7I 142 CTCGAG C^TCGAG
173 CTCGAG
PshBI 358 ATTAAT AT^TAAT
1302 ATTAAT
RcaI 1098 TCATGA T^CATGA
1586 TCATGA
RsaI 1325 GTAC GT^AC
1674 GTAC
SacI 132 GAGCTC GAGCT^C
2068 GAGCTC
XhoI 142 CTCGAG C^TCGAG
173 CTCGAG
XhoII 1284 GGATCT [GA]^GATC[CT]
2004 AGATCT
XmnI 768 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1102 GAACGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AcsI AflIII AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I