Restriction map for
pFN21AA0820
(Linear) (Six-base) MAPSORT of: KIAA0820.seq Check: 8026 from: 1 to: 2577
KIAA0820.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 836 2577
Size: 836 1741
AccI GT'mk_AC
Cuts at: 0 1775 1826 1967 2577
Size: 1775 51 141 610
Fragments arranged by size:
1775 610 141 51
AclI AA'CG_TT
Cuts at: 0 2045 2577
Size: 2045 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 870 2075 2577
Size: 580 290 1205 502
Fragments arranged by size:
1205 580 502 290
AflIII A'CryG_T
Cuts at: 0 53 381 383 1976 2577
Size: 53 328 2 1593 601
Fragments arranged by size:
1593 601 328 53 2
AleI CACnn'nnGTG
Cuts at: 0 1391 2577
Size: 1391 1186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1760 2577
Size: 1760 817
AlwNI CAG_nnn'CTG
Cuts at: 0 1355 2359 2577
Size: 1355 1004 218
Fragments arranged by size:
1355 1004 218
ApaI G_GGCC'C
Cuts at: 0 2493 2577
Size: 2493 84
ApoI r'AATT_y
Cuts at: 0 268 945 2061 2577
Size: 268 677 1116 516
Fragments arranged by size:
1116 677 516 268
AseI AT'TA_AT
Cuts at: 0 359 1303 2577
Size: 359 944 1274
Fragments arranged by size:
1274 944 359
AvaI C'yCGr_G
Cuts at: 0 142 173 2577
Size: 142 31 2404
Fragments arranged by size:
2404 142 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1980 2013 2577
Size: 1980 33 564
Fragments arranged by size:
1980 564 33
BanI G'GyrC_C
Cuts at: 0 2229 2577
Size: 2229 348
BanII G_rGCy'C
Cuts at: 0 136 2072 2394 2460 2493 2577
Size: 136 1936 322 66 33 84
Fragments arranged by size:
1936 322 136 84 66 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 979 1414 1478 2577
Size: 965 14 435 64 1099
Fragments arranged by size:
1099 965 435 64 14
BciVI GTATCCnnnnn_n'
Cuts at: 0 864 1600 2008 2249 2577
Size: 864 736 408 241 328
Fragments arranged by size:
864 736 408 328 241
BclI T'GATC_A
Cuts at: 0 2036 2577
Size: 2036 541
BglI GCCn_nnn'nGGC
Cuts at: 0 757 2577
Size: 757 1820
BglII A'GATC_T
Cuts at: 0 2004 2577
Size: 2004 573
BlpI GC'TnA_GC
Cuts at: 0 1496 2126 2135 2577
Size: 1496 630 9 442
Fragments arranged by size:
1496 630 442 9
Bme1580I G_kGCm'C
Cuts at: 0 2493 2577
Size: 2493 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 2492 2577
Size: 2492 85
BmtI G_CTAG'C
Cuts at: 0 568 2577
Size: 568 2009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 509 1196 2222 2381 2577
Size: 106 403 687 1026 159 196
Fragments arranged by size:
1026 687 403 196 159 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 569 1816 2577
Size: 316 253 1247 761
Fragments arranged by size:
1247 761 316 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 1942 2508 2577
Size: 1942 566 69
Fragments arranged by size:
1942 566 69
BsaAI yAC'GTr
Cuts at: 0 384 693 2577
Size: 384 309 1884
Fragments arranged by size:
1884 384 309
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 1518 2270 2384 2577
Size: 34 1484 752 114 193
Fragments arranged by size:
1484 752 193 114 34
BseYI C'CCAG_C
Cuts at: 0 66 1855 2434 2577
Size: 66 1789 579 143
Fragments arranged by size:
1789 579 143 66
BsiEI CG_ry'CG
Cuts at: 0 2346 2577
Size: 2346 231
BsiHKAI G_wGCw'C
Cuts at: 0 136 143 2072 2322 2577
Size: 136 7 1929 250 255
Fragments arranged by size:
1929 255 250 136 7
Bsp1286I G_dGCh'C
Cuts at: 0 136 143 2072 2322 2394 2460 2493 2577
Size: 136 7 1929 250 72 66 33 84
Fragments arranged by size:
1929 250 136 84 72 66 33 7
BspHI T'CATG_A
Cuts at: 0 1098 1586 2577
Size: 1098 488 991
Fragments arranged by size:
1098 991 488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 836 2261 2577
Size: 836 1425 316
Fragments arranged by size:
1425 836 316
BsrDI GCAATG_nn'
Cuts at: 0 252 982 2577
Size: 252 730 1595
Fragments arranged by size:
1595 730 252
BsrFI r'CCGG_y
Cuts at: 0 124 2577
Size: 124 2453
BssSI C'ACGA_G
Cuts at: 0 492 2577
Size: 492 2085
BstAPI GCAn_nnn'nTGC
Cuts at: 0 238 2577
Size: 238 2339
BstBI TT'CG_AA
Cuts at: 0 2157 2577
Size: 2157 420
BstEII G'GTnAC_C
Cuts at: 0 2273 2577
Size: 2273 304
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1335 2577
Size: 1335 1242
BstYI r'GATC_y
Cuts at: 0 1284 2004 2577
Size: 1284 720 573
Fragments arranged by size:
1284 720 573
BstZ17I GTA'TAC
Cuts at: 0 1776 2577
Size: 1776 801
BtgI C'CryG_G
Cuts at: 0 104 1919 2577
Size: 104 1815 658
Fragments arranged by size:
1815 658 104
BtsI GCAGTG_nn'
Cuts at: 0 2448 2577
Size: 2448 129
DraIII CAC_nnn'GTG
Cuts at: 0 2317 2577
Size: 2317 260
DrdI GACnn_nn'nnGTC
Cuts at: 0 1444 2577
Size: 1444 1133
EaeI y'GGCC_r
Cuts at: 0 114 2210 2343 2577
Size: 114 2096 133 234
Fragments arranged by size:
2096 234 133 114
EagI C'GGCC_G
Cuts at: 0 2343 2577
Size: 2343 234
EarI CTCTTCn'nnn_
Cuts at: 0 683 1018 1149 1270 2577
Size: 683 335 131 121 1307
Fragments arranged by size:
1307 683 335 131 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2539 2577
Size: 2539 38
EcoICRI GAG'CTC
Cuts at: 0 134 2070 2577
Size: 134 1936 507
Fragments arranged by size:
1936 507 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 509 580 870 1196 2075 2222 2381
Size: 106 403 71 290 326 879 147 159
Cuts at: 2381 2577
Size: 196
Fragments arranged by size:
879 403 326 290 196 159 147 106
71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 832 2113 2577
Size: 832 1281 464
Fragments arranged by size:
1281 832 464
EcoO109I rG'GnC_Cy
Cuts at: 0 837 1264 2489 2490 2577
Size: 837 427 1225 1 87
Fragments arranged by size:
1225 837 427 87 1
EcoRV GAT'ATC
Cuts at: 0 453 2577
Size: 453 2124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 310 1758 2200 2577
Size: 310 1448 442 377
Fragments arranged by size:
1448 442 377 310
HaeII r_GCGC'y
Cuts at: 0 67 125 2142 2577
Size: 67 58 2017 435
Fragments arranged by size:
2017 435 67 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 896 928 1269 1301 1494 1526 1844 1876
Size: 896 32 341 32 193 32 318 32
Cuts at: 1876 2577
Size: 701
Fragments arranged by size:
896 701 341 318 193 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1827 2577
Size: 1827 750
HindIII A'AGCT_T
Cuts at: 0 1632 2577
Size: 1632 945
MfeI C'AATT_G
Cuts at: 0 1930 2577
Size: 1930 647
MluI A'CGCG_T
Cuts at: 0 53 2577
Size: 53 2524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1515 2577
Size: 1515 1062
MslI CAynn'nnrTG
Cuts at: 0 1391 2577
Size: 1391 1186
MspA1I CmG'CkG
Cuts at: 0 33 1832 2577
Size: 33 1799 745
Fragments arranged by size:
1799 745 33
NaeI GCC'GGC
Cuts at: 0 126 2577
Size: 126 2451
NcoI C'CATG_G
Cuts at: 0 1919 2577
Size: 1919 658
NgoMIV G'CCGG_C
Cuts at: 0 124 2577
Size: 124 2453
NheI G'CTAG_C
Cuts at: 0 564 2577
Size: 564 2013
NspI r_CATG'y
Cuts at: 0 1680 1808 1980 2577
Size: 1680 128 172 597
Fragments arranged by size:
1680 597 172 128
PciI A'CATG_T
Cuts at: 0 1976 2577
Size: 1976 601
PfoI T'CCnGG_A
Cuts at: 0 2251 2577
Size: 2251 326
PmlI CAC'GTG
Cuts at: 0 384 2577
Size: 384 2193
PpuMI rG'GwC_Cy
Cuts at: 0 837 2577
Size: 837 1740
PspOMI G'GGCC_C
Cuts at: 0 2489 2577
Size: 2489 88
PspXI vC'TCGA_Gb
Cuts at: 0 173 2577
Size: 173 2404
PstI C_TGCA'G
Cuts at: 0 50 215 833 992 2577
Size: 50 165 618 159 1585
Fragments arranged by size:
1585 618 165 159 50
PvuII CAG'CTG
Cuts at: 0 1832 2577
Size: 1832 745
SacI G_AGCT'C
Cuts at: 0 136 2072 2577
Size: 136 1936 505
Fragments arranged by size:
1936 505 136
SalI G'TCGA_C
Cuts at: 0 1825 2577
Size: 1825 752
SexAI A'CCwGG_T
Cuts at: 0 1418 2577
Size: 1418 1159
SfcI C'TryA_G
Cuts at: 0 46 211 829 898 988 1877 2577
Size: 46 165 618 69 90 889 700
Fragments arranged by size:
889 700 618 165 90 69 46
SmlI C'TyrA_G
Cuts at: 0 142 173 295 584 1795 2577
Size: 142 31 122 289 1211 782
Fragments arranged by size:
1211 782 289 142 122 31
StuI AGG'CCT
Cuts at: 0 2481 2577
Size: 2481 96
StyI C'CwwG_G
Cuts at: 0 1685 1919 2184 2577
Size: 1685 234 265 393
Fragments arranged by size:
1685 393 265 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 824 2577
Size: 824 1753
TatI w'GTAC_w
Cuts at: 0 1673 2577
Size: 1673 904
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1529 1561 2577
Size: 1529 32 1016
Fragments arranged by size:
1529 1016 32
XbaI T'CTAG_A
Cuts at: 0 657 2577
Size: 657 1920
XhoI C'TCGA_G
Cuts at: 0 142 173 2577
Size: 142 31 2404
Fragments arranged by size:
2404 142 31
XmnI GAAnn'nnTTC
Cuts at: 0 772 1106 2577
Size: 772 334 1471
Fragments arranged by size:
1471 772 334
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApoI AseI AvaI BaeI BanI BanII BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BpmI BpuEI BsaI BsaAI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII MfeI MluI
MmeI MslI MspA1I NaeI NcoI NgoMIV NheI NspI
PciI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII
SacI SalI SexAI SfcI SmlI StuI StyI TaqII
TatI TstI XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AloI ApaLI
AscI AsiSI AvrII BamHI BbeI BbvCI BcgI BfrBI
BmgBI BplI Bpu10I BsaBI BsaHI BsaWI BsaXI BsgI
BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII Bsu36I
BtgZI ClaI CspCI DraI EcoRI FseI FspI FspAI
HpaI KasI KpnI MscI NarI NdeI NotI NruI
NsiI PacI PasI PflMI PmeI PpiI PshAI PsiI
PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XcmI XmaI ZraI