(Circular) (Six-base) MAPSORT of: pFN21AA0808.seq Check: 6441 from: 1 to: 6283 pFN21AA0808 6283 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4093 278 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3626 1937 Size: 1689 4594 Acc65I G'GTAC_C Cuts at: 3604 3604 Size: 6283 AclI AA'CG_TT Cuts at: 1563 4410 4783 1563 Size: 2847 373 3063 Fragments arranged by size: 3063 2847 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2990 4346 5730 5998 1632 Size: 1358 1356 1384 268 1917 Fragments arranged by size: 1917 1384 1358 1356 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6086 AflIII A'CryG_T Cuts at: 1203 2121 5182 6159 1203 Size: 918 3061 977 1327 Fragments arranged by size: 3061 1327 977 918 AgeI A'CCGG_T Cuts at: 3514 5114 3514 Size: 1600 4683 AhdI GACnn_n'nnGTC Cuts at: 5012 5012 Size: 6283 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6283 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6283 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2586 2618 2586 Size: 32 6251 AlwNI CAG_nnn'CTG Cuts at: 3224 4026 5598 3224 Size: 802 1572 3909 Fragments arranged by size: 3909 1572 802 ApaI G_GGCC'C Cuts at: 3090 3090 Size: 6283 ApaLI G'TGCA_C Cuts at: 2255 4340 5496 2255 Size: 2085 1156 3042 Fragments arranged by size: 3042 2085 1156 ApoI r'AATT_y Cuts at: 1493 3592 3818 3970 6079 1493 Size: 2099 226 152 2109 1697 Fragments arranged by size: 2109 2099 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4837 160 Size: 872 3805 1606 Fragments arranged by size: 3805 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 AvaI C'yCGr_G Cuts at: 1870 1943 3608 1870 Size: 73 1665 4545 Fragments arranged by size: 4545 1665 73 BamHI G'GATC_C Cuts at: 2773 3613 3980 2773 Size: 840 367 5076 Fragments arranged by size: 5076 840 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3604 5059 618 Size: 325 217 627 1817 1455 1842 Fragments arranged by size: 1842 1817 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2787 3090 3602 1391 Size: 1396 303 512 4072 Fragments arranged by size: 4072 1396 512 303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2208 3041 928 Size: 971 309 833 4170 Fragments arranged by size: 4170 971 833 309 BbvCI CC'TCA_GC Cuts at: 3051 3051 Size: 6283 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4474 4508 1903 Size: 34 2537 34 3678 Fragments arranged by size: 3678 2537 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3013 4175 5385 3013 Size: 1162 1210 3911 Fragments arranged by size: 3911 1210 1162 BclI T'GATC_A Cuts at: 1548 3057 1548 Size: 1509 4774 BfrBI ATG'CAT Cuts at: 3169 3169 Size: 6283 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3104 4894 136 Size: 107 122 71 631 605 1432 1790 1525 Fragments arranged by size: 1790 1525 1432 631 605 122 107 71 BglII A'GATC_T Cuts at: 6278 6278 Size: 6283 BlpI GC'TnA_GC Cuts at: 3700 3700 Size: 6283 Bme1580I G_kGCm'C Cuts at: 1790 2259 3090 4344 5500 1790 Size: 469 831 1254 1156 2573 Fragments arranged by size: 2573 1254 1156 831 469 BmgBI CAC'GTC Cuts at: 1318 3031 1318 Size: 1713 4570 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4014 4972 442 Size: 416 53 83 399 24 2597 958 1753 Fragments arranged by size: 2597 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2013 3251 4943 1239 Size: 774 1238 1692 2579 Fragments arranged by size: 2579 1692 1238 774 Bpu10I CC'TnA_GC Cuts at: 3051 3051 Size: 6283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4408 5273 5571 4408 Size: 865 298 5120 Fragments arranged by size: 5120 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2473 4946 882 Size: 617 974 2473 2219 Fragments arranged by size: 2473 2219 974 617 BsaAI yAC'GTr Cuts at: 493 1204 4074 493 Size: 711 2870 2702 Fragments arranged by size: 2870 2702 711 BsaBI GATnn'nnATC Cuts at: 6277 6277 Size: 6283 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4090 4472 5107 275 Size: 53 83 186 936 2557 382 635 1451 Fragments arranged by size: 2557 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3514 4716 5114 5388 5535 5946 3514 Size: 1202 398 274 147 411 3851 Fragments arranged by size: 3851 1202 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6058 Fragments arranged by size: 6058 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2217 3039 3246 3249 1186 Size: 1031 822 207 3 4220 Fragments arranged by size: 4220 1031 822 207 3 BseYI C'CCAG_C Cuts at: 2034 2374 3089 5486 5982 2034 Size: 340 715 2397 496 2335 Fragments arranged by size: 2397 2335 715 496 340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3435 3514 1163 Size: 573 1699 79 3932 Fragments arranged by size: 3932 1699 573 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3733 4494 5522 1540 Size: 275 186 1732 761 1028 2301 Fragments arranged by size: 2301 1732 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 2572 4344 4429 5500 2259 Size: 313 1772 85 1071 3042 Fragments arranged by size: 3042 1772 1071 313 85 BsmI GAATG_Cn' Cuts at: 3794 3887 3794 Size: 93 6190 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2572 2787 3090 3602 4344 4429 Size: 399 469 313 215 303 512 742 85 Cuts at: 4429 5500 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 512 469 399 313 303 215 85 BspEI T'CCGG_A Cuts at: 5946 5946 Size: 6283 BspHI T'CATG_A Cuts at: 4172 5902 4172 Size: 1730 4553 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3349 3638 844 Size: 2505 289 3489 Fragments arranged by size: 3489 2505 289 BsrBI CCG'CTC Cuts at: 1394 1823 3319 4170 1394 Size: 429 1496 851 3507 Fragments arranged by size: 3507 1496 851 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2605 3477 4778 4952 65 Size: 1183 1357 872 1301 174 1396 Fragments arranged by size: 1396 1357 1301 1183 872 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3514 4927 5114 1700 Size: 110 1704 1413 187 2869 Fragments arranged by size: 2869 1704 1413 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6283 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6283 BssSI C'ACGA_G Cuts at: 4343 5355 4343 Size: 1012 5271 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6283 BstBI TT'CG_AA Cuts at: 3065 5087 3065 Size: 2022 4261 BstEII G'GTnAC_C Cuts at: 1184 2279 1184 Size: 1095 5188 BstXI CCAn_nnnn'nTGG Cuts at: 2374 2374 Size: 6283 BstYI r'GATC_y Cuts at: 1972 2773 3613 3980 4365 4382 5834 5920 5932 Size: 801 840 367 385 17 1452 86 12 Cuts at: 5932 6278 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 840 801 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3082 513 Size: 552 939 1078 3714 Fragments arranged by size: 3714 1078 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2697 487 Size: 683 56 1471 4073 Fragments arranged by size: 4073 1471 683 56 BtsI GCAGTG_nn' Cuts at: 2390 3799 4593 4613 2390 Size: 1409 794 20 4060 Fragments arranged by size: 4060 1409 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6248 DraI TTT'AAA Cuts at: 3587 3587 Size: 6283 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5290 809 Size: 507 3974 1802 Fragments arranged by size: 3974 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2533 3730 4619 Size: 54 1425 50 275 18 703 1197 889 Cuts at: 4619 6248 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1197 889 703 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3730 1537 Size: 275 1918 4090 Fragments arranged by size: 4090 1918 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2910 3288 3411 4213 1353 Size: 1557 378 123 802 3423 Fragments arranged by size: 3423 1557 802 378 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4870 5244 5390 387 Size: 1064 138 3281 374 146 1280 Fragments arranged by size: 3281 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2013 2990 3251 4346 4943 5730 5998 Size: 393 381 977 261 1095 597 787 268 Cuts at: 5998 1239 Size: 1524 Fragments arranged by size: 1524 1095 977 787 597 393 381 268 261 EcoO109I rG'GnC_Cy Cuts at: 2069 2069 Size: 6283 EcoRI G'AATT_C Cuts at: 3592 3970 3592 Size: 378 5905 FspI TGC'GCA Cuts at: 4789 4789 Size: 6283 HaeII r_GCGC'y Cuts at: 1678 5430 1678 Size: 3752 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2675 2707 4924 4956 4998 Size: 32 135 32 1103 32 2217 32 42 Cuts at: 4998 5030 1373 Size: 32 2626 Fragments arranged by size: 2626 2217 1103 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3028 3627 3873 669 Size: 988 281 1090 599 246 3079 Fragments arranged by size: 3079 1090 988 599 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6283 HpaI GTT'AAC Cuts at: 3873 3873 Size: 6283 KpnI G_GTAC'C Cuts at: 3608 3608 Size: 6283 MfeI C'AATT_G Cuts at: 3882 3882 Size: 6283 MluI A'CGCG_T Cuts at: 6159 6159 Size: 6283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5397 5581 1305 Size: 4092 184 2007 Fragments arranged by size: 4092 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2535 10 Size: 54 1425 343 703 3758 Fragments arranged by size: 3758 1425 703 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2262 2411 2673 3245 4241 4600 Size: 648 960 136 149 262 572 996 359 Cuts at: 4600 4759 518 Size: 159 2042 Fragments arranged by size: 2042 996 960 648 572 359 262 159 149 136 MspA1I CmG'CkG Cuts at: 1606 2045 2539 3055 3449 3699 4376 5524 5769 Size: 439 494 516 394 250 677 1148 245 Cuts at: 5769 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 516 494 439 394 250 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3082 513 Size: 552 939 1078 3714 Fragments arranged by size: 3714 1078 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6283 NotI GC'GGCC_GC Cuts at: 3730 3730 Size: 6283 NsiI A_TGCA'T Cuts at: 3171 3171 Size: 6283 NspI r_CATG'y Cuts at: 1132 1229 2125 3304 3556 3641 5186 1132 Size: 97 896 1179 252 85 1545 2229 Fragments arranged by size: 2229 1545 1179 896 252 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6283 PciI A'CATG_T Cuts at: 2121 5182 2121 Size: 3061 3222 PflMI CCAn_nnn'nTGG Cuts at: 1108 2251 3472 1108 Size: 1143 1221 3919 Fragments arranged by size: 3919 1221 1143 PmeI GTTT'AAAC Cuts at: 3587 3587 Size: 6283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4352 4384 5865 5897 4352 Size: 32 1481 32 4738 Fragments arranged by size: 4738 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2069 2069 Size: 6283 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6283 PsiI TTA'TAA Cuts at: 3853 3853 Size: 6283 PspOMI G'GGCC_C Cuts at: 3086 3086 Size: 6283 PstI C_TGCA'G Cuts at: 830 2500 3542 3635 830 Size: 1670 1042 93 3478 Fragments arranged by size: 3478 1670 1042 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6283 PvuII CAG'CTG Cuts at: 2539 3055 3449 2539 Size: 516 394 5373 Fragments arranged by size: 5373 516 394 SalI G'TCGA_C Cuts at: 1936 3625 1936 Size: 1689 4594 SapI GCTCTTCn'nnn_ Cuts at: 2910 2910 Size: 6283 SbfI CC_TGCA'GG Cuts at: 3635 3635 Size: 6283 ScaI AGT'ACT Cuts at: 4531 6243 4531 Size: 1712 4571 SfcI C'TryA_G Cuts at: 826 1045 2496 3013 3231 3444 3538 3631 4766 Size: 219 1451 517 218 213 94 93 1135 Cuts at: 4766 5447 5638 826 Size: 681 191 1471 Fragments arranged by size: 1471 1451 1135 681 517 219 218 213 191 94 93 SfiI GGCCn_nnn'nGGCC Cuts at: 3104 3104 Size: 6283 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6283 SmaI CCC'GGG Cuts at: 1872 3610 1872 Size: 1738 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4387 5288 5550 820 Size: 197 926 2444 901 262 1553 Fragments arranged by size: 2444 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4074 493 Size: 3581 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6283 SphI G_CATG'C Cuts at: 1132 3556 3641 1132 Size: 2424 85 3774 Fragments arranged by size: 3774 2424 85 SspI AAT'ATT Cuts at: 5 52 4207 5 Size: 47 4155 2081 Fragments arranged by size: 4155 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6283 StyI C'CwwG_G Cuts at: 513 1065 2004 2483 3082 3337 3722 513 Size: 552 939 479 599 255 385 3074 Fragments arranged by size: 3074 939 599 552 479 385 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3397 3479 4310 4327 4480 4665 5110 Size: 1255 1817 82 831 17 153 185 445 Cuts at: 5110 325 Size: 1498 Fragments arranged by size: 1817 1498 1255 831 445 185 153 82 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 4529 6241 Size: 274 80 33 51 1443 471 2081 1712 Cuts at: 6241 96 Size: 138 Fragments arranged by size: 2081 1712 1443 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6243 6275 6243 Size: 32 6251 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6283 XbaI T'CTAG_A Cuts at: 3619 3619 Size: 6283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2177 2177 Size: 6283 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6283 XmaI C'CCGG_G Cuts at: 1870 3608 1870 Size: 1738 4545 XmnI GAAnn'nnTTC Cuts at: 4412 4412 Size: 6283 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4091 276 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI