Restriction map for
pFN21AA0804
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccII AclI AflII
AgeI Alw44I ApaI ApaLI AscI
Asp718I AvaI AviII AvrII BbeI
BbrPI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsiEI BsiWI BsoBI
BspDI BsrBI BsrFI BssHII Bst1107I
BstBI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoNI EcoT22I FauI FseI
FspI HpaI KasI KpnI KspI
MluI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 2684 TCCGGA T^CCGGA
AcyI 3794 GACGCC G[GA]^CG[CT]C
AflIII 1164 ACATGT A^C[GA][CT]GT
AhdI 2618 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2299 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1953 AGCGCT AGC^GCT
AseI 1935 ATTAAT AT^TAAT
AsnI 1935 ATTAAT AT^TAAT
AspEI 2618 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 554 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1670 GAGCTC G[GA]GC[CT]^C
BbsI 3014 GTCTTC GAAGAC(2/6)
BbvCI 1482 GCTGAGG CCTCAGC(-5/-2)
BciVI 484 GTATCC GTATCC(6/5)
BcnI 2833 CCCGG CC^[GC]GG
BpuAI 3014 GTCTTC GAAGAC(2/6)
BsaAI 2774 TACGTG [CT]AC^GT[GA]
BsaHI 3794 GACGCC G[GA]^CG[CT]C
BsaI 3358 GAGACC GGTCTC(1/5)
BseAI 2684 TCCGGA T^CCGGA
BsgI 2843 GTGCAG GTGCAG(16/14)
Bsp1407I 1962 TGTACA T^GTACA
BspEI 2684 TCCGGA T^CCGGA
BspHI 3198 TCATGA T^CATGA
BspLU11I 1164 ACATGT A^CATGT
BsrGI 1962 TGTACA T^GTACA
BssSI 2896 CTCGTG CACGAG(-5/-1)
BstEII 3732 GGTCACC G^GT[ACGT]ACC
BstPI 3732 GGTCACC G^GT[ACGT]ACC
BstXI 2940 CCACTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2299 CCTGAGG CC^T[ACGT]AGG
DraIII 663 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 950 CCATGG C^C[GA][CT]GG
Eam1105I 2618 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1953 AGCGCT AGC^GCT
Eco81I 2299 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3732 GGTCACC G^GT[ACGT]ACC
EcoRV 1972 GATATC GAT^ATC
HgaI 3794 GACGC GACGC(5/10)
Hin1I 3794 GACGCC G[GA]^CG[CT]C
HincII 2675 GTCGAC GT[CT]^[GA]AC
HindII 2675 GTCGAC GT[CT]^[GA]AC
MfeI 476 CAATTG C^AATTG
MroI 2684 TCCGGA T^CCGGA
MunI 476 CAATTG C^AATTG
NciI 2833 CCCGG CC^[GC]GG
NcoI 950 CCATGG C^CATGG
NdeI 1966 CATATG CA^TATG
PflFI 554 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1935 ATTAAT AT^TAAT
RcaI 3198 TCATGA T^CATGA
SacI 1670 GAGCTC GAGCT^C
SalI 2675 GTCGAC G^TCGAC
SapI 61 GAAGAGC GCTCTTC(1/4)
SpeI 571 ACTAGT A^CTAGT
SspBI 1962 TGTACA T^GTACA
Tth111I 554 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 1734 GGTGCC G^G[CT][GA]CC
3244 GGTGCC
BsaBI 535 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
709 GATGCTCATC
BsaWI 1634 ACCGGA [AT]^CCGG[AT]
2684 TCCGGA
BsmBI 4108 CGTCTC CGTCTC(1/5)
3792 GAGACG
BstAPI 2194 GCAGGTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3538 GCATTTATTGC
DraI 2389 TTTAAA TTT^AAA
3522 TTTAAA
EarI 61 GAAGAG CTCTTC(1/4)
2668 GAAGAG
HaeII 592 GGCGCT [GA]GCGC^[CT]
1953 AGCGCT
HindIII 89 AAGCTT A^AGCTT
545 AAGCTT
Ksp632I 61 GAAGAG CTCTTC(1/4)
2668 GAAGAG
MaeII 2775 ACGT A^CGT
3408 ACGT
MamI 535 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
709 GATGCTCATC
PpuMI 2567 AGGTCCT [GA]G^G[AT]CC[CT]
2624 AGGTCCT
SexAI 2252 ACCAGGT A^CC[AT]GGT
2984 ACCAGGT
SspI 528 AATATT AAT^ATT
732 AATATT
TaqI 1668 TCGA T^CGA
2676 TCGA
TthHB8I 1668 TCGA T^CGA
2676 TCGA
XbaI 84 TCTAGA T^CTAGA
844 TCTAGA
XcmI 665 CCATGTGGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1048 CCACTGCTGGCCTGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BamHI BbvI BclI
BfaI BglII BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmnI