(Circular) (Six-base) MAPSORT of: pFN21AA0804.seq Check: 2833 from: 1 to: 8983 pFN21AA0804 8983 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6793 278 Size: 53 83 186 936 5257 2468 Fragments arranged by size: 5257 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3336 4053 4681 6326 1937 Size: 1399 717 628 1645 4594 Fragments arranged by size: 4594 1645 1399 717 628 Acc65I G'GTAC_C Cuts at: 6304 6304 Size: 8983 AclI AA'CG_TT Cuts at: 1563 7110 7483 1563 Size: 5547 373 3063 Fragments arranged by size: 5547 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4251 4931 4967 5517 5525 7046 8430 8698 Size: 2619 680 36 550 8 1521 1384 268 Cuts at: 8698 1632 Size: 1917 Fragments arranged by size: 2619 1917 1521 1384 680 550 268 36 8 AfeI AGC'GCT Cuts at: 3960 3960 Size: 8983 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8786 AflIII A'CryG_T Cuts at: 1203 3169 7882 8859 1203 Size: 1966 4713 977 1327 Fragments arranged by size: 4713 1966 1327 977 AgeI A'CCGG_T Cuts at: 7814 7814 Size: 8983 AhdI GACnn_n'nnGTC Cuts at: 4628 7712 4628 Size: 3084 5899 AleI CACnn'nnGTG Cuts at: 1166 3242 1166 Size: 2076 6907 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3260 1184 Size: 2076 6907 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2126 2158 3111 3143 4647 4679 2126 Size: 32 953 32 1504 32 6430 Fragments arranged by size: 6430 1504 953 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3391 5464 6068 6726 8298 3391 Size: 2073 604 658 1572 4076 Fragments arranged by size: 4076 2073 1572 658 604 ApaLI G'TGCA_C Cuts at: 7040 8196 7040 Size: 1156 7827 ApoI r'AATT_y Cuts at: 1493 2108 2424 3229 3820 4073 4268 4358 4465 Size: 615 316 805 591 253 195 90 107 Cuts at: 4465 4580 4719 4727 5006 5386 5888 5959 6292 Size: 115 139 8 279 380 502 71 333 Cuts at: 6292 6518 6670 8779 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 805 615 591 502 380 333 316 279 253 226 195 152 139 115 107 90 71 8 AseI AT'TA_AT Cuts at: 160 1032 3941 7537 160 Size: 872 2909 3596 1606 Fragments arranged by size: 3596 2909 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8983 AvaI C'yCGr_G Cuts at: 1870 1943 6308 1870 Size: 73 4365 4545 Fragments arranged by size: 4545 4365 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5343 5376 5343 Size: 33 8950 BamHI G'GATC_C Cuts at: 2260 3037 5326 5584 6037 6076 6313 6680 2260 Size: 777 2289 258 453 39 237 367 4563 Fragments arranged by size: 4563 2289 777 453 367 258 237 39 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3739 5249 6304 7759 618 Size: 325 217 627 1952 1510 1055 1455 1842 Fragments arranged by size: 1952 1842 1510 1455 1055 627 325 217 BanII G_rGCy'C Cuts at: 1391 3679 6302 1391 Size: 2288 2623 4072 Fragments arranged by size: 4072 2623 2288 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5012 928 Size: 971 3113 4899 Fragments arranged by size: 4899 3113 971 BbvCI CC'TCA_GC Cuts at: 3488 3488 Size: 8983 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7174 7208 1903 Size: 34 5237 34 3678 Fragments arranged by size: 5237 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2500 6875 8085 2500 Size: 4375 1210 3398 Fragments arranged by size: 4375 3398 1210 BclI T'GATC_A Cuts at: 1548 2539 4567 6100 1548 Size: 991 2028 1533 4431 Fragments arranged by size: 4431 2028 1533 991 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7594 136 Size: 107 122 71 631 605 5922 1525 Fragments arranged by size: 5922 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3315 3447 4234 5701 8978 3315 Size: 132 787 1467 3277 3320 Fragments arranged by size: 3320 3277 1467 787 132 BlpI GC'TnA_GC Cuts at: 6400 6400 Size: 8983 Bme1580I G_kGCm'C Cuts at: 1790 3692 7044 8200 1790 Size: 1902 3352 1156 2573 Fragments arranged by size: 3352 2573 1902 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6714 7672 442 Size: 416 53 83 399 24 5297 958 1753 Fragments arranged by size: 5297 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8983 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5347 5347 Size: 8983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2709 3180 3390 3517 4674 6115 7643 Size: 920 550 471 210 127 1157 1441 1528 Cuts at: 7643 1239 Size: 2579 Fragments arranged by size: 2579 1528 1441 1157 920 550 471 210 127 Bpu10I CC'TnA_GC Cuts at: 3488 3488 Size: 8983 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2169 2276 2617 3875 5171 7108 7973 8271 2169 Size: 107 341 1258 1296 1937 865 298 2881 Fragments arranged by size: 2881 1937 1296 1258 865 341 298 107 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5357 7646 882 Size: 617 3858 2289 2219 Fragments arranged by size: 3858 2289 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4781 6774 493 Size: 711 3577 1993 2702 Fragments arranged by size: 3577 2702 1993 711 BsaBI GATnn'nnATC Cuts at: 2544 2718 8977 2544 Size: 174 6259 2550 Fragments arranged by size: 6259 2550 174 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5800 6790 7172 7807 Size: 53 83 186 936 4267 990 382 635 Cuts at: 7807 275 Size: 1451 Fragments arranged by size: 4267 1451 990 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3639 4689 7416 7814 8088 8235 8646 3639 Size: 1050 2727 398 274 147 411 3976 Fragments arranged by size: 3976 2727 1050 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2784 2814 3111 3141 3363 Size: 30 165 30 1381 30 297 30 222 Cuts at: 3363 3393 4589 4619 4647 4677 4847 4877 1178 Size: 30 1196 30 28 30 170 30 5284 Fragments arranged by size: 5284 1381 1196 297 222 170 165 30 30 30 30 30 30 30 30 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2187 3132 3505 4104 4107 4868 5029 6134 Size: 1001 945 373 599 3 761 161 1105 Cuts at: 6134 1186 Size: 4035 Fragments arranged by size: 4035 1105 1001 945 761 599 373 161 3 BseYI C'CCAG_C Cuts at: 8186 8682 8186 Size: 496 8487 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4869 1163 Size: 573 3133 5277 Fragments arranged by size: 5277 3133 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6433 7194 8222 1540 Size: 275 186 4432 761 1028 2301 Fragments arranged by size: 4432 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3249 3679 6211 7044 7129 8200 3249 Size: 430 2532 833 85 1071 4032 Fragments arranged by size: 4032 2532 1071 833 430 85 BsmI GAATG_Cn' Cuts at: 2902 4129 4285 5882 6494 6587 2902 Size: 1227 156 1597 612 93 5298 Fragments arranged by size: 5298 1597 1227 612 156 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5791 6119 5791 Size: 328 8655 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3249 3679 3692 6211 6302 7044 7129 Size: 399 1459 430 13 2519 91 742 85 Cuts at: 7129 8200 1391 Size: 1071 2174 Fragments arranged by size: 2519 2174 1459 1071 742 430 399 91 85 13 BspEI T'CCGG_A Cuts at: 4689 8646 4689 Size: 3957 5026 BspHI T'CATG_A Cuts at: 5203 6872 8602 5203 Size: 1669 1730 5584 Fragments arranged by size: 5584 1730 1669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3991 4190 4618 6338 844 Size: 3147 199 428 1720 3489 Fragments arranged by size: 3489 3147 1720 428 199 BsrBI CCG'CTC Cuts at: 1394 1823 6870 1394 Size: 429 5047 3507 Fragments arranged by size: 5047 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4107 4887 5367 5451 7478 7652 65 Size: 1183 2859 780 480 84 2027 174 1396 Fragments arranged by size: 2859 2027 1396 1183 780 480 174 84 BsrFI r'CCGG_y Cuts at: 1700 1810 7627 7814 1700 Size: 110 5817 187 2869 Fragments arranged by size: 5817 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3967 96 Size: 3871 5112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8983 BssSI C'ACGA_G Cuts at: 4901 7043 8055 4901 Size: 2142 1012 5829 Fragments arranged by size: 5829 2142 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4205 5549 794 Size: 3411 1344 4228 Fragments arranged by size: 4228 3411 1344 BstBI TT'CG_AA Cuts at: 7787 7787 Size: 8983 BstEII G'GTnAC_C Cuts at: 1184 5737 1184 Size: 4553 4430 BstXI CCAn_nnnn'nTGG Cuts at: 4952 4952 Size: 8983 BstYI r'GATC_y Cuts at: 1972 2260 3037 3315 3447 3781 4234 5326 5584 Size: 288 777 278 132 334 453 1092 258 Cuts at: 5584 5701 6037 6076 6313 6680 7065 7082 8534 Size: 117 336 39 237 367 385 17 1452 Cuts at: 8534 8620 8632 8978 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1092 777 453 385 367 346 336 334 288 278 258 237 132 117 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 4305 4305 Size: 8983 BtgI C'CryG_G Cuts at: 513 1065 2004 2955 513 Size: 552 939 951 6541 Fragments arranged by size: 6541 951 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8244 Fragments arranged by size: 8244 683 56 BtsI GCAGTG_nn' Cuts at: 2511 3053 6042 6499 7293 7313 2511 Size: 542 2989 457 794 20 4181 Fragments arranged by size: 4181 2989 794 542 457 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8948 DraI TTT'AAA Cuts at: 4396 5529 6287 4396 Size: 1133 758 7092 Fragments arranged by size: 7092 1133 758 DraIII CAC_nnn'GTG Cuts at: 2673 2673 Size: 8983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7990 809 Size: 507 6674 1802 Fragments arranged by size: 6674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2682 2952 3028 Size: 54 1425 50 275 18 852 270 76 Cuts at: 3028 3828 5839 6430 7319 8948 8 Size: 800 2011 591 889 1629 43 Fragments arranged by size: 2011 1629 1425 889 852 800 591 275 270 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6430 1537 Size: 275 4618 4090 Fragments arranged by size: 4618 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2061 4668 6913 1353 Size: 708 2607 2245 3423 Fragments arranged by size: 3423 2607 2245 708 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7570 7944 8090 387 Size: 1064 138 5981 374 146 1280 Fragments arranged by size: 5981 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3677 3677 Size: 8983 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2709 3180 3390 3517 4251 4674 Size: 393 527 550 471 210 127 734 423 Cuts at: 4674 4931 4967 5517 5525 6115 7046 7643 8430 Size: 257 36 550 8 590 931 597 787 Cuts at: 8430 8698 1239 Size: 268 1524 Fragments arranged by size: 1524 931 787 734 597 590 550 550 527 471 423 393 268 257 210 127 36 8 EcoO109I rG'GnC_Cy Cuts at: 4573 4630 5419 4573 Size: 57 789 8137 Fragments arranged by size: 8137 789 57 EcoRI G'AATT_C Cuts at: 3820 4465 4580 6292 6670 3820 Size: 645 115 1712 378 6133 Fragments arranged by size: 6133 1712 645 378 115 EcoRV GAT'ATC Cuts at: 3979 3979 Size: 8983 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2117 3551 5624 2117 Size: 1434 2073 5476 Fragments arranged by size: 5476 2073 1434 FspI TGC'GCA Cuts at: 7489 7489 Size: 8983 HaeII r_GCGC'y Cuts at: 1678 2601 3962 8130 1678 Size: 923 1361 4168 2531 Fragments arranged by size: 4168 2531 1361 923 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2297 2314 2329 3496 Size: 32 135 32 710 15 17 15 1167 Cuts at: 3496 3528 4589 4614 4621 4646 4847 4879 5356 Size: 32 1061 25 7 25 201 32 477 Cuts at: 5356 5388 5440 5472 5721 5753 5756 5788 7624 Size: 32 52 32 249 32 3 32 1836 Cuts at: 7624 7656 7698 7730 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1836 1167 1061 710 477 249 201 135 52 42 32 32 32 32 32 32 32 32 32 32 25 25 17 15 15 7 3 HincII GTy'rAC Cuts at: 669 1657 1938 4682 6327 6573 669 Size: 988 281 2744 1645 246 3079 Fragments arranged by size: 3079 2744 1645 988 281 246 HindIII A'AGCT_T Cuts at: 748 2094 2550 748 Size: 1346 456 7181 Fragments arranged by size: 7181 1346 456 HpaI GTT'AAC Cuts at: 6573 6573 Size: 8983 KpnI G_GTAC'C Cuts at: 6308 6308 Size: 8983 MfeI C'AATT_G Cuts at: 2481 6582 2481 Size: 4101 4882 MluI A'CGCG_T Cuts at: 8859 8859 Size: 8983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2225 2563 2789 3382 8097 8281 1305 Size: 920 338 226 593 4715 184 2007 Fragments arranged by size: 4715 2007 920 593 338 226 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2684 2954 3830 5841 10 Size: 54 1425 343 852 270 876 2011 3152 Fragments arranged by size: 3152 2011 1425 876 852 343 270 54 MslI CAynn'nnrTG Cuts at: 518 1166 2028 2123 2301 3242 4691 4752 5307 Size: 648 862 95 178 941 1449 61 555 Cuts at: 5307 6941 7300 7459 518 Size: 1634 359 159 2042 Fragments arranged by size: 2042 1634 1449 941 862 648 555 359 178 159 95 61 MspA1I CmG'CkG Cuts at: 1606 2450 2790 3331 6250 6399 7076 8224 8469 Size: 844 340 541 2919 149 677 1148 245 Cuts at: 8469 1606 Size: 2120 Fragments arranged by size: 2919 2120 1148 844 677 541 340 245 149 NcoI C'CATG_G Cuts at: 513 1065 2004 2955 513 Size: 552 939 951 6541 Fragments arranged by size: 6541 951 939 552 NdeI CA'TA_TG Cuts at: 387 3972 387 Size: 3585 5398 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8983 NotI GC'GGCC_GC Cuts at: 6430 6430 Size: 8983 NspI r_CATG'y Cuts at: 1132 1229 3173 3275 5916 6341 7886 1132 Size: 97 1944 102 2641 425 1545 2229 Fragments arranged by size: 2641 2229 1944 1545 425 102 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8983 PciI A'CATG_T Cuts at: 3169 7882 3169 Size: 4713 4270 PflMI CCAn_nnn'nTGG Cuts at: 1108 3391 3715 5594 5809 6194 1108 Size: 2283 324 1879 215 385 3897 Fragments arranged by size: 3897 2283 1879 385 324 215 PfoI T'CCnGG_A Cuts at: 2725 4837 2725 Size: 2112 6871 PmeI GTTT'AAAC Cuts at: 6287 6287 Size: 8983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2028 2060 3111 3143 4647 4679 7052 7084 8565 Size: 32 1051 32 1504 32 2373 32 1481 Cuts at: 8565 8597 2028 Size: 32 2414 Fragments arranged by size: 2414 2373 1504 1481 1051 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4573 4630 4573 Size: 57 8926 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8983 PsiI TTA'TAA Cuts at: 6553 6553 Size: 8983 PstI C_TGCA'G Cuts at: 830 2449 3116 3774 4650 6255 6335 830 Size: 1619 667 658 876 1605 80 3478 Fragments arranged by size: 3478 1619 1605 876 667 658 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8983 PvuII CAG'CTG Cuts at: 2450 3331 6250 2450 Size: 881 2919 5183 Fragments arranged by size: 5183 2919 881 SacI G_AGCT'C Cuts at: 3679 3679 Size: 8983 SalI G'TCGA_C Cuts at: 1936 4680 6325 1936 Size: 2744 1645 4594 Fragments arranged by size: 4594 2744 1645 SapI GCTCTTCn'nnn_ Cuts at: 2061 2061 Size: 8983 SbfI CC_TGCA'GG Cuts at: 6335 6335 Size: 8983 ScaI AGT'ACT Cuts at: 7231 8943 7231 Size: 1712 7271 SexAI A'CCwGG_T Cuts at: 4257 4989 4257 Size: 732 8251 SfcI C'TryA_G Cuts at: 826 1045 2445 2475 3112 3770 4646 6181 6251 Size: 219 1400 30 637 658 876 1535 70 Cuts at: 6251 6331 7466 8147 8338 826 Size: 80 1135 681 191 1471 Fragments arranged by size: 1535 1471 1400 1135 876 681 658 637 219 191 80 70 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8983 SmaI CCC'GGG Cuts at: 1872 6310 1872 Size: 4438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2184 2255 2632 3854 5150 7087 Size: 197 926 241 71 377 1222 1296 1937 Cuts at: 7087 7988 8250 820 Size: 901 262 1553 Fragments arranged by size: 1937 1553 1296 1222 926 901 377 262 241 197 71 SnaBI TAC'GTA Cuts at: 493 6774 493 Size: 6281 2702 SpeI A'CTAG_T Cuts at: 152 2576 152 Size: 2424 6559 SphI G_CATG'C Cuts at: 1132 6341 1132 Size: 5209 3774 SspI AAT'ATT Cuts at: 5 52 2535 2739 6907 5 Size: 47 2483 204 4168 2081 Fragments arranged by size: 4168 2483 2081 204 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8983 StyI C'CwwG_G Cuts at: 513 1065 2004 2871 2955 3697 4216 4245 5030 Size: 552 939 867 84 742 519 29 785 Cuts at: 5030 6422 513 Size: 1392 3074 Fragments arranged by size: 3074 1392 939 867 785 742 552 519 84 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4958 5725 7010 7027 7180 7365 7810 Size: 1255 3378 767 1285 17 153 185 445 Cuts at: 7810 325 Size: 1498 Fragments arranged by size: 3378 1498 1285 1255 767 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3079 3967 5790 Size: 274 80 33 51 1443 1102 888 1823 Cuts at: 5790 7229 8941 96 Size: 1439 1712 138 Fragments arranged by size: 1823 1712 1443 1439 1102 888 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6072 6104 8943 8975 6072 Size: 32 2839 32 6080 Fragments arranged by size: 6080 2839 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2562 1316 Size: 1246 7737 XbaI T'CTAG_A Cuts at: 2089 2849 6319 2089 Size: 760 3470 4753 Fragments arranged by size: 4753 3470 760 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2677 3060 2677 Size: 383 8600 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8983 XmaI C'CCGG_G Cuts at: 1870 6308 1870 Size: 4438 4545 XmnI GAAnn'nnTTC Cuts at: 4097 5040 5175 6069 7112 4097 Size: 943 135 894 1043 5968 Fragments arranged by size: 5968 1043 943 894 135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6791 276 Size: 53 83 186 936 5257 2468 Fragments arranged by size: 5257 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI