Restriction map for
pFN21AA0804
(Linear) (Six-base) MAPSORT of: KIAA0804.seq Check: 1779 from: 1 to: 4278
KIAA0804.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 1331 2048 2676 4278
Size: 1331 717 628 1602
Fragments arranged by size:
1602 1331 717 628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2246 2926 2962 3512 3520 4278
Size: 2246 680 36 550 8 758
Fragments arranged by size:
2246 758 680 550 36 8
AfeI AGC'GCT
Cuts at: 0 1955 4278
Size: 1955 2323
AflIII A'CryG_T
Cuts at: 0 1164 4278
Size: 1164 3114
AhdI GACnn_n'nnGTC
Cuts at: 0 2623 4278
Size: 2623 1655
AleI CACnn'nnGTG
Cuts at: 0 1237 4278
Size: 1237 3041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1255 4278
Size: 1255 3023
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 121 153 1106 1138 2642 2674 4278
Size: 121 32 953 32 1504 32 1604
Fragments arranged by size:
1604 1504 953 121 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1386 3459 4063 4278
Size: 1386 2073 604 215
Fragments arranged by size:
2073 1386 604 215
ApoI r'AATT_y
Cuts at: 0 103 419 1224 1815 2068 2263 2353 2460
Size: 103 316 805 591 253 195 90 107
Cuts at: 2460 2575 2714 2722 3001 3381 3883 3954 4278
Size: 115 139 8 279 380 502 71 324
Fragments arranged by size:
805 591 502 380 324 316 279 253
195 139 115 107 103 90 71 8
AseI AT'TA_AT
Cuts at: 0 1936 4278
Size: 1936 2342
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3338 3371 4278
Size: 3338 33 907
Fragments arranged by size:
3338 907 33
BamHI G'GATC_C
Cuts at: 0 255 1032 3321 3579 4032 4071 4278
Size: 255 777 2289 258 453 39 207
Fragments arranged by size:
2289 777 453 258 255 207 39
BanI G'GyrC_C
Cuts at: 0 1734 3244 4278
Size: 1734 1510 1034
Fragments arranged by size:
1734 1510 1034
BanII G_rGCy'C
Cuts at: 0 1674 4278
Size: 1674 2604
BbsI GAAGACnn'nnnn_
Cuts at: 0 3007 4278
Size: 3007 1271
BbvCI CC'TCA_GC
Cuts at: 0 1483 4278
Size: 1483 2795
BciVI GTATCCnnnnn_n'
Cuts at: 0 495 4278
Size: 495 3783
BclI T'GATC_A
Cuts at: 0 534 2562 4095 4278
Size: 534 2028 1533 183
Fragments arranged by size:
2028 1533 534 183
BglII A'GATC_T
Cuts at: 0 1310 1442 2229 3696 4278
Size: 1310 132 787 1467 582
Fragments arranged by size:
1467 1310 787 582 132
Bme1580I G_kGCm'C
Cuts at: 0 1687 4278
Size: 1687 2591
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3342 4278
Size: 3342 936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 704 1175 1385 1512 2669 4110 4278
Size: 154 550 471 210 127 1157 1441 168
Fragments arranged by size:
1441 1157 550 471 210 168 154 127
Bpu10I CC'TnA_GC
Cuts at: 0 1483 4278
Size: 1483 2795
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 271 612 1870 3166 4278
Size: 164 107 341 1258 1296 1112
Fragments arranged by size:
1296 1258 1112 341 164 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 3352 4278
Size: 3352 926
BsaAI yAC'GTr
Cuts at: 0 2776 4278
Size: 2776 1502
BsaBI GATnn'nnATC
Cuts at: 0 539 713 4278
Size: 539 174 3565
Fragments arranged by size:
3565 539 174
BsaHI Gr'CG_yC
Cuts at: 0 3795 4278
Size: 3795 483
BsaWI w'CCGG_w
Cuts at: 0 1634 2684 4278
Size: 1634 1050 1594
Fragments arranged by size:
1634 1594 1050
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 779 809 1106 1136 1358 1388 2584 2614
Size: 779 30 297 30 222 30 1196 30
Cuts at: 2614 2642 2672 2842 2872 4278
Size: 28 30 170 30 1406
Fragments arranged by size:
1406 1196 779 297 222 170 30 30
30 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 182 1127 1500 2099 2102 2863 3024 4129
Size: 182 945 373 599 3 761 161 1105
Cuts at: 4129 4278
Size: 149
Fragments arranged by size:
1105 945 761 599 373 182 161 149
3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2864 4278
Size: 2864 1414
BsiHKAI G_wGCw'C
Cuts at: 0 1244 1674 4206 4278
Size: 1244 430 2532 72
Fragments arranged by size:
2532 1244 430 72
BsmI GAATG_Cn'
Cuts at: 0 897 2124 2280 3877 4278
Size: 897 1227 156 1597 401
Fragments arranged by size:
1597 1227 897 401 156
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3786 4114 4278
Size: 3786 328 164
Fragments arranged by size:
3786 328 164
Bsp1286I G_dGCh'C
Cuts at: 0 1244 1674 1687 4206 4278
Size: 1244 430 13 2519 72
Fragments arranged by size:
2519 1244 430 72 13
BspEI T'CCGG_A
Cuts at: 0 2684 4278
Size: 2684 1594
BspHI T'CATG_A
Cuts at: 0 3198 4278
Size: 3198 1080
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1986 2185 2613 4278
Size: 1986 199 428 1665
Fragments arranged by size:
1986 1665 428 199
BsrDI GCAATG_nn'
Cuts at: 0 2102 2882 3362 3446 4278
Size: 2102 780 480 84 832
Fragments arranged by size:
2102 832 780 480 84
BsrGI T'GTAC_A
Cuts at: 0 1962 4278
Size: 1962 2316
BssSI C'ACGA_G
Cuts at: 0 2896 4278
Size: 2896 1382
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2200 3544 4278
Size: 2200 1344 734
Fragments arranged by size:
2200 1344 734
BstEII G'GTnAC_C
Cuts at: 0 3732 4278
Size: 3732 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2947 4278
Size: 2947 1331
BstYI r'GATC_y
Cuts at: 0 255 1032 1310 1442 1776 2229 3321 3579
Size: 255 777 278 132 334 453 1092 258
Cuts at: 3579 3696 4032 4071 4278
Size: 117 336 39 207
Fragments arranged by size:
1092 777 453 336 334 278 258 255
207 132 117 39
Bsu36I CC'TnA_GG
Cuts at: 0 2300 4278
Size: 2300 1978
BtgI C'CryG_G
Cuts at: 0 950 4278
Size: 950 3328
BtsI GCAGTG_nn'
Cuts at: 0 506 1048 4037 4278
Size: 506 542 2989 241
Fragments arranged by size:
2989 542 506 241
DraI TTT'AAA
Cuts at: 0 2391 3524 4278
Size: 2391 1133 754
Fragments arranged by size:
2391 1133 754
DraIII CAC_nnn'GTG
Cuts at: 0 668 4278
Size: 668 3610
EaeI y'GGCC_r
Cuts at: 0 677 947 1023 1823 3834 4278
Size: 677 270 76 800 2011 444
Fragments arranged by size:
2011 800 677 444 270 76
EarI CTCTTCn'nnn_
Cuts at: 0 56 2663 4278
Size: 56 2607 1615
Fragments arranged by size:
2607 1615 56
EcoICRI GAG'CTC
Cuts at: 0 1672 4278
Size: 1672 2606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 704 1175 1385 1512 2246 2669 2926
Size: 154 550 471 210 127 734 423 257
Cuts at: 2926 2962 3512 3520 4110 4278
Size: 36 550 8 590 168
Fragments arranged by size:
734 590 550 550 471 423 257 210
168 154 127 36 8
EcoO109I rG'GnC_Cy
Cuts at: 0 2568 2625 3414 4278
Size: 2568 57 789 864
Fragments arranged by size:
2568 864 789 57
EcoRI G'AATT_C
Cuts at: 0 1815 2460 2575 4278
Size: 1815 645 115 1703
Fragments arranged by size:
1815 1703 645 115
EcoRV GAT'ATC
Cuts at: 0 1974 4278
Size: 1974 2304
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 112 1546 3619 4278
Size: 112 1434 2073 659
Fragments arranged by size:
2073 1434 659 112
HaeII r_GCGC'y
Cuts at: 0 596 1957 4278
Size: 596 1361 2321
Fragments arranged by size:
2321 1361 596
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 292 309 324 1491 1523 2584 2609
Size: 277 15 17 15 1167 32 1061 25
Cuts at: 2609 2616 2641 2842 2874 3351 3383 3435 3467
Size: 7 25 201 32 477 32 52 32
Cuts at: 3467 3716 3748 3751 3783 4278
Size: 249 32 3 32 495
Fragments arranged by size:
1167 1061 495 477 277 249 201 52
32 32 32 32 32 32 25 25
17 15 15 7 3
HincII GTy'rAC
Cuts at: 0 2677 4278
Size: 2677 1601
HindIII A'AGCT_T
Cuts at: 0 89 545 4278
Size: 89 456 3733
Fragments arranged by size:
3733 456 89
MfeI C'AATT_G
Cuts at: 0 476 4278
Size: 476 3802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 558 784 1377 4278
Size: 220 338 226 593 2901
Fragments arranged by size:
2901 593 338 226 220
MscI TGG'CCA
Cuts at: 0 679 949 1825 3836 4278
Size: 679 270 876 2011 442
Fragments arranged by size:
2011 876 679 442 270
MslI CAynn'nnrTG
Cuts at: 0 23 118 296 1237 2686 2747 3302 4278
Size: 23 95 178 941 1449 61 555 976
Fragments arranged by size:
1449 976 941 555 178 95 61 23
MspA1I CmG'CkG
Cuts at: 0 445 785 1326 4245 4278
Size: 445 340 541 2919 33
Fragments arranged by size:
2919 541 445 340 33
NcoI C'CATG_G
Cuts at: 0 950 4278
Size: 950 3328
NdeI CA'TA_TG
Cuts at: 0 1967 4278
Size: 1967 2311
NspI r_CATG'y
Cuts at: 0 1168 1270 3911 4278
Size: 1168 102 2641 367
Fragments arranged by size:
2641 1168 367 102
PciI A'CATG_T
Cuts at: 0 1164 4278
Size: 1164 3114
PflMI CCAn_nnn'nTGG
Cuts at: 0 1386 1710 3589 3804 4189 4278
Size: 1386 324 1879 215 385 89
Fragments arranged by size:
1879 1386 385 324 215 89
PfoI T'CCnGG_A
Cuts at: 0 720 2832 4278
Size: 720 2112 1446
Fragments arranged by size:
2112 1446 720
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 1106 1138 2642 2674 4278
Size: 23 32 1051 32 1504 32 1604
Fragments arranged by size:
1604 1504 1051 32 32 32 23
PpuMI rG'GwC_Cy
Cuts at: 0 2568 2625 4278
Size: 2568 57 1653
Fragments arranged by size:
2568 1653 57
PstI C_TGCA'G
Cuts at: 0 444 1111 1769 2645 4250 4278
Size: 444 667 658 876 1605 28
Fragments arranged by size:
1605 876 667 658 444 28
PvuII CAG'CTG
Cuts at: 0 445 1326 4245 4278
Size: 445 881 2919 33
Fragments arranged by size:
2919 881 445 33
SacI G_AGCT'C
Cuts at: 0 1674 4278
Size: 1674 2604
SalI G'TCGA_C
Cuts at: 0 2675 4278
Size: 2675 1603
SapI GCTCTTCn'nnn_
Cuts at: 0 56 4278
Size: 56 4222
SexAI A'CCwGG_T
Cuts at: 0 2252 2984 4278
Size: 2252 732 1294
Fragments arranged by size:
2252 1294 732
SfcI C'TryA_G
Cuts at: 0 440 470 1107 1765 2641 4176 4246 4278
Size: 440 30 637 658 876 1535 70 32
Fragments arranged by size:
1535 876 658 637 440 70 32 30
SmlI C'TyrA_G
Cuts at: 0 179 250 627 1849 3145 4278
Size: 179 71 377 1222 1296 1133
Fragments arranged by size:
1296 1222 1133 377 179 71
SpeI A'CTAG_T
Cuts at: 0 571 4278
Size: 571 3707
SspI AAT'ATT
Cuts at: 0 530 734 4278
Size: 530 204 3544
Fragments arranged by size:
3544 530 204
StyI C'CwwG_G
Cuts at: 0 866 950 1692 2211 2240 3025 4278
Size: 866 84 742 519 29 785 1253
Fragments arranged by size:
1253 866 785 742 519 84 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2953 3720 4278
Size: 2953 767 558
Fragments arranged by size:
2953 767 558
TatI w'GTAC_w
Cuts at: 0 1074 1962 3785 4278
Size: 1074 888 1823 493
Fragments arranged by size:
1823 1074 888 493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4067 4099 4278
Size: 4067 32 179
Fragments arranged by size:
4067 179 32
Tth111I GACn'n_nGTC
Cuts at: 0 557 4278
Size: 557 3721
XbaI T'CTAG_A
Cuts at: 0 84 844 4278
Size: 84 760 3434
Fragments arranged by size:
3434 760 84
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 672 1055 4278
Size: 672 383 3223
Fragments arranged by size:
3223 672 383
XmnI GAAnn'nnTTC
Cuts at: 0 2092 3035 3170 4064 4278
Size: 2092 943 135 894 214
Fragments arranged by size:
2092 943 894 214 135
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApoI AseI BaeI BamHI BanI BanII BbsI
BbvCI BciVI BclI BglII Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI
PciI PflMI PfoI PpiI PpuMI PstI PvuII SacI
SalI SapI SexAI SfcI SmlI SpeI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI ApaI ApaLI
AscI AsiSI AvaI AvrII BbeI BcgI BfrBI BglI
BlpI BmgBI BmrI BmtI BseYI BsiEI BsiWI BsrBI
BsrFI BssHII BstBI BstZ17I BtgZI ClaI CspCI DrdI
EagI EciI EcoNI FseI FspI FspAI HpaI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI XhoI XmaI ZraI