(Circular) (Six-base) MAPSORT of: pFN21AA0740.seq Check: 8104 from: 1 to: 6793 pFN21AA0740 6793 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2652 2652 Size: 6793 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4603 278 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2985 4136 1937 Size: 1048 1151 4594 Fragments arranged by size: 4594 1151 1048 Acc65I G'GTAC_C Cuts at: 3969 4114 3969 Size: 145 6648 AclI AA'CG_TT Cuts at: 1563 2037 3452 4920 5293 1563 Size: 474 1415 1468 373 3063 Fragments arranged by size: 3063 1468 1415 474 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2675 2804 3201 3995 4856 6240 6508 1632 Size: 1043 129 397 794 861 1384 268 1917 Fragments arranged by size: 1917 1384 1043 861 794 397 268 129 AfeI AGC'GCT Cuts at: 2625 2625 Size: 6793 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6596 AflIII A'CryG_T Cuts at: 1203 5692 6669 1203 Size: 4489 977 1327 Fragments arranged by size: 4489 1327 977 AgeI A'CCGG_T Cuts at: 2725 5624 2725 Size: 2899 3894 AhdI GACnn_n'nnGTC Cuts at: 5522 5522 Size: 6793 AleI CACnn'nnGTG Cuts at: 1166 2150 1166 Size: 984 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2660 1184 Size: 1476 5317 AlwNI CAG_nnn'CTG Cuts at: 2140 3101 4536 6108 2140 Size: 961 1435 1572 2825 Fragments arranged by size: 2825 1572 1435 961 ApaI G_GGCC'C Cuts at: 3014 3192 3014 Size: 178 6615 ApaLI G'TGCA_C Cuts at: 4850 6006 4850 Size: 1156 5637 ApoI r'AATT_y Cuts at: 1493 2736 2850 2876 4072 4102 4328 4480 6589 Size: 1243 114 26 1196 30 226 152 2109 Cuts at: 6589 1493 Size: 1697 Fragments arranged by size: 2109 1697 1243 1196 226 152 114 30 26 AseI AT'TA_AT Cuts at: 160 1032 3680 5347 160 Size: 872 2648 1667 1606 Fragments arranged by size: 2648 1667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 AvaI C'yCGr_G Cuts at: 1870 1943 2211 3323 4118 1870 Size: 73 268 1112 795 4545 Fragments arranged by size: 4545 1112 795 268 73 AvrII C'CTAG_G Cuts at: 3017 3017 Size: 6793 BamHI G'GATC_C Cuts at: 4123 4490 4123 Size: 367 6426 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2421 2472 2640 2695 3948 Size: 325 217 627 634 51 168 55 1253 Cuts at: 3948 3969 4114 5569 618 Size: 21 145 1455 1842 Fragments arranged by size: 1842 1455 1253 634 627 325 217 168 145 55 51 21 BanII G_rGCy'C Cuts at: 1391 3014 3192 4112 1391 Size: 1623 178 920 4072 Fragments arranged by size: 4072 1623 920 178 BbeI G_GCGC'C Cuts at: 2476 2476 Size: 6793 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2090 3039 928 Size: 971 191 949 4682 Fragments arranged by size: 4682 971 949 191 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4044 4078 4984 5018 1903 Size: 34 2107 34 906 34 3678 Fragments arranged by size: 3678 2107 906 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2248 2382 4685 5895 2248 Size: 134 2303 1210 3146 Fragments arranged by size: 3146 2303 1210 134 BclI T'GATC_A Cuts at: 1548 2266 1548 Size: 718 6075 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5404 136 Size: 107 122 71 631 605 3732 1525 Fragments arranged by size: 3732 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6788 6788 Size: 6793 BlpI GC'TnA_GC Cuts at: 4210 4210 Size: 6793 Bme1580I G_kGCm'C Cuts at: 1790 3014 3192 3499 4854 6010 1790 Size: 1224 178 307 1355 1156 2573 Fragments arranged by size: 2573 1355 1224 1156 307 178 BmgBI CAC'GTC Cuts at: 1318 3463 1318 Size: 2145 4648 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4524 5482 442 Size: 416 53 83 399 24 3107 958 1753 Fragments arranged by size: 3107 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6793 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2256 3812 2256 Size: 1556 5237 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3233 4065 5453 1239 Size: 1994 832 1388 2579 Fragments arranged by size: 2579 1994 1388 832 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4033 4918 5783 6081 4033 Size: 885 865 298 4745 Fragments arranged by size: 4745 885 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5456 882 Size: 617 3957 2219 Fragments arranged by size: 3957 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2152 3409 4584 493 Size: 711 948 1257 1175 2702 Fragments arranged by size: 2702 1257 1175 948 711 BsaBI GATnn'nnATC Cuts at: 6787 6787 Size: 6793 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2473 4600 4982 5617 Size: 53 83 186 936 940 2127 382 635 Cuts at: 5617 275 Size: 1451 Fragments arranged by size: 2127 1451 940 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2725 3119 5226 5624 5898 6045 6456 2725 Size: 394 2107 398 274 147 411 3062 Fragments arranged by size: 3062 2107 411 398 394 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6568 Fragments arranged by size: 6568 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3020 1186 Size: 1834 4959 BseYI C'CCAG_C Cuts at: 5996 6492 5996 Size: 496 6297 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3114 3850 1163 Size: 573 1378 736 4106 Fragments arranged by size: 4106 1378 736 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4243 5004 6032 1540 Size: 275 186 2242 761 1028 2301 Fragments arranged by size: 2301 2242 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3798 4854 4939 6010 3798 Size: 1056 85 1071 4581 Fragments arranged by size: 4581 1071 1056 85 BsmI GAATG_Cn' Cuts at: 4304 4397 4304 Size: 93 6700 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3014 3192 3499 3798 4112 4854 4939 Size: 399 1224 178 307 299 314 742 85 Cuts at: 4939 6010 1391 Size: 1071 2174 Fragments arranged by size: 2174 1224 1071 742 399 314 307 299 178 85 BspEI T'CCGG_A Cuts at: 6456 6456 Size: 6793 BspHI T'CATG_A Cuts at: 3360 3378 4682 6412 3360 Size: 18 1304 1730 3741 Fragments arranged by size: 3741 1730 1304 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2289 2652 4148 844 Size: 1445 363 1496 3489 Fragments arranged by size: 3489 1496 1445 363 BsrBI CCG'CTC Cuts at: 1394 1823 3946 4680 1394 Size: 429 2123 734 3507 Fragments arranged by size: 3507 2123 734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5288 5462 65 Size: 1183 4040 174 1396 Fragments arranged by size: 4040 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2725 5437 5624 1700 Size: 110 915 2712 187 2869 Fragments arranged by size: 2869 2712 915 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6793 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6793 BssSI C'ACGA_G Cuts at: 3766 4853 5865 3766 Size: 1087 1012 4694 Fragments arranged by size: 4694 1087 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6793 BstBI TT'CG_AA Cuts at: 5597 5597 Size: 6793 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6793 BstXI CCAn_nnnn'nTGG Cuts at: 2163 3199 2163 Size: 1036 5757 BstYI r'GATC_y Cuts at: 1972 3661 4123 4490 4875 4892 6344 6430 6442 Size: 1689 462 367 385 17 1452 86 12 Cuts at: 6442 6788 1972 Size: 346 1977 Fragments arranged by size: 1977 1689 1452 462 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3027 3027 Size: 6793 BtgI C'CryG_G Cuts at: 513 1065 2004 2076 2754 513 Size: 552 939 72 678 4552 Fragments arranged by size: 4552 939 678 552 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4028 487 Size: 683 56 2802 3252 Fragments arranged by size: 3252 2802 683 56 BtsI GCAGTG_nn' Cuts at: 4092 4309 5103 5123 4092 Size: 217 794 20 5762 Fragments arranged by size: 5762 794 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6793 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6758 DraI TTT'AAA Cuts at: 3472 4097 3472 Size: 625 6168 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3218 5800 809 Size: 507 1902 2582 1802 Fragments arranged by size: 2582 1902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 3840 4089 Size: 54 1425 50 275 18 312 1698 249 Cuts at: 4089 4240 5129 6758 8 Size: 151 889 1629 43 Fragments arranged by size: 1698 1629 1425 889 312 275 249 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4240 1537 Size: 275 2428 4090 Fragments arranged by size: 4090 2428 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2872 4723 1353 Size: 1519 1851 3423 Fragments arranged by size: 3423 1851 1519 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2986 5380 5754 5900 387 Size: 1064 138 1397 2394 374 146 1280 Fragments arranged by size: 2394 1397 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2675 2804 3201 3233 3995 4065 4856 Size: 393 1043 129 397 32 762 70 791 Cuts at: 4856 5453 6240 6508 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1043 791 787 762 597 397 393 268 129 70 32 EcoNI CCTnn'n_nnAGG Cuts at: 3065 3065 Size: 6793 EcoO109I rG'GnC_Cy Cuts at: 3010 3188 3230 3319 3010 Size: 178 42 89 6484 Fragments arranged by size: 6484 178 89 42 EcoRI G'AATT_C Cuts at: 4072 4102 4480 4072 Size: 30 378 6385 Fragments arranged by size: 6385 378 30 EcoRV GAT'ATC Cuts at: 3606 3606 Size: 6793 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2107 3391 4081 2107 Size: 1284 690 4819 Fragments arranged by size: 4819 1284 690 FspI TGC'GCA Cuts at: 5299 5299 Size: 6793 HaeII r_GCGC'y Cuts at: 1678 2476 2627 5940 1678 Size: 798 151 3313 2531 Fragments arranged by size: 3313 2531 798 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2320 2352 5434 5466 5508 Size: 32 135 32 748 32 3082 32 42 Cuts at: 5508 5540 1373 Size: 32 2626 Fragments arranged by size: 3082 2626 748 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2986 3166 3753 4137 4383 669 Size: 988 281 1048 180 587 384 246 3079 Fragments arranged by size: 3079 1048 988 587 384 281 246 180 HindIII A'AGCT_T Cuts at: 748 748 Size: 6793 HpaI GTT'AAC Cuts at: 4383 4383 Size: 6793 KasI G'GCGC_C Cuts at: 2472 2472 Size: 6793 KpnI G_GTAC'C Cuts at: 3973 4118 3973 Size: 145 6648 MfeI C'AATT_G Cuts at: 4392 4392 Size: 6793 MluI A'CGCG_T Cuts at: 6669 6669 Size: 6793 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5907 6091 1305 Size: 4602 184 2007 Fragments arranged by size: 4602 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 10 Size: 54 1425 343 312 4659 Fragments arranged by size: 4659 1425 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 3143 3197 4751 5110 5269 518 Size: 648 984 993 54 1554 359 159 2042 Fragments arranged by size: 2042 1554 993 984 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2137 3508 3673 3954 4209 4886 6034 6279 Size: 531 1371 165 281 255 677 1148 245 Cuts at: 6279 1606 Size: 2120 Fragments arranged by size: 2120 1371 1148 677 531 281 255 245 165 NarI GG'CG_CC Cuts at: 2473 2473 Size: 6793 NcoI C'CATG_G Cuts at: 513 1065 2004 2754 513 Size: 552 939 750 4552 Fragments arranged by size: 4552 939 750 552 NdeI CA'TA_TG Cuts at: 387 2293 387 Size: 1906 4887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6793 NotI GC'GGCC_GC Cuts at: 4240 4240 Size: 6793 NspI r_CATG'y Cuts at: 1132 1229 2368 3148 3740 4151 5696 1132 Size: 97 1139 780 592 411 1545 2229 Fragments arranged by size: 2229 1545 1139 780 592 411 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6793 PciI A'CATG_T Cuts at: 5692 5692 Size: 6793 PflMI CCAn_nnn'nTGG Cuts at: 1108 3838 1108 Size: 2730 4063 PmeI GTTT'AAAC Cuts at: 4097 4097 Size: 6793 PmlI CAC'GTG Cuts at: 2152 2152 Size: 6793 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4862 4894 6375 6407 4862 Size: 32 1481 32 5248 Fragments arranged by size: 5248 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3319 3319 Size: 6793 PshAI GACnn'nnGTC Cuts at: 1613 3200 1613 Size: 1587 5206 PsiI TTA'TAA Cuts at: 4363 4363 Size: 6793 PspOMI G'GGCC_C Cuts at: 3010 3188 3010 Size: 178 6615 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2535 2567 2535 Size: 32 6761 PstI C_TGCA'G Cuts at: 830 3925 4145 830 Size: 3095 220 3478 Fragments arranged by size: 3478 3095 220 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6793 PvuII CAG'CTG Cuts at: 2137 2137 Size: 6793 SalI G'TCGA_C Cuts at: 1936 2984 4135 1936 Size: 1048 1151 4594 Fragments arranged by size: 4594 1151 1048 SbfI CC_TGCA'GG Cuts at: 4145 4145 Size: 6793 ScaI AGT'ACT Cuts at: 5041 6753 5041 Size: 1712 5081 SfcI C'TryA_G Cuts at: 826 1045 3517 3921 4141 5276 5957 6148 826 Size: 219 2472 404 220 1135 681 191 1471 Fragments arranged by size: 2472 1471 1135 681 404 220 219 191 SfoI GGC'GCC Cuts at: 2474 2474 Size: 6793 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6793 SmaI CCC'GGG Cuts at: 1872 4120 1872 Size: 2248 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3323 4048 4897 5798 6060 820 Size: 197 926 1380 725 849 901 262 1553 Fragments arranged by size: 1553 1380 926 901 849 725 262 197 SnaBI TAC'GTA Cuts at: 493 4584 493 Size: 4091 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6793 SphI G_CATG'C Cuts at: 1132 3148 3740 4151 1132 Size: 2016 592 411 3774 Fragments arranged by size: 3774 2016 592 411 SspI AAT'ATT Cuts at: 5 52 4717 5 Size: 47 4665 2081 Fragments arranged by size: 4665 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6793 StyI C'CwwG_G Cuts at: 513 1065 2004 2754 3017 3687 4232 513 Size: 552 939 750 263 670 545 3074 Fragments arranged by size: 3074 939 750 670 552 545 263 SwaI ATTT'AAAT Cuts at: 3472 3472 Size: 6793 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 2162 2409 4820 4837 4990 5175 Size: 1255 474 108 247 2411 17 153 185 Cuts at: 5175 5620 325 Size: 445 1498 Fragments arranged by size: 2411 1498 1255 474 445 247 185 153 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5039 6751 96 Size: 274 80 33 51 1443 3062 1712 138 Fragments arranged by size: 3062 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2394 2426 2965 2997 6753 6785 2394 Size: 32 539 32 3756 32 2402 Fragments arranged by size: 3756 2402 539 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6793 XbaI T'CTAG_A Cuts at: 4129 4129 Size: 6793 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3772 3772 Size: 6793 XhoI C'TCGA_G Cuts at: 1943 3323 1943 Size: 1380 5413 XmaI C'CCGG_G Cuts at: 1870 4118 1870 Size: 2248 4545 XmnI GAAnn'nnTTC Cuts at: 4922 4922 Size: 6793 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4601 276 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI