Restriction map for
pFN21AA0737
(Linear) (Six-base) MAPSORT of: KIAA0737.seq Check: 3054 from: 1 to: 1863
KIAA0737.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 501 1863
Size: 501 1362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 871 1863
Size: 527 344 992
Fragments arranged by size:
992 527 344
AleI CACnn'nnGTG
Cuts at: 0 1180 1849 1863
Size: 1180 669 14
Fragments arranged by size:
1180 669 14
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 502 1863
Size: 502 1361
AlwNI CAG_nnn'CTG
Cuts at: 0 1285 1288 1863
Size: 1285 3 575
Fragments arranged by size:
1285 575 3
ApoI r'AATT_y
Cuts at: 0 106 1836 1863
Size: 106 1730 27
Fragments arranged by size:
1730 106 27
AvaI C'yCGr_G
Cuts at: 0 1190 1863
Size: 1190 673
AvrII C'CTAG_G
Cuts at: 0 468 1863
Size: 468 1395
BamHI G'GATC_C
Cuts at: 0 915 1863
Size: 915 948
BanI G'GyrC_C
Cuts at: 0 478 1590 1863
Size: 478 1112 273
Fragments arranged by size:
1112 478 273
BanII G_rGCy'C
Cuts at: 0 296 1001 1199 1863
Size: 296 705 198 664
Fragments arranged by size:
705 664 296 198
BbsI GAAGACnn'nnnn_
Cuts at: 0 601 1863
Size: 601 1262
BclI T'GATC_A
Cuts at: 0 432 1619 1863
Size: 432 1187 244
Fragments arranged by size:
1187 432 244
BmrI ACTGGGnnnn_n'
Cuts at: 0 219 492 1229 1767 1863
Size: 219 273 737 538 96
Fragments arranged by size:
737 538 273 219 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1505 1700 1863
Size: 1505 195 163
Fragments arranged by size:
1505 195 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1091 1390 1863
Size: 962 129 299 473
Fragments arranged by size:
962 473 299 129
Bpu10I CC'TnA_GC
Cuts at: 0 1547 1863
Size: 1547 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1130 1863
Size: 1130 733
BsaI GGTCTCn'nnnn_
Cuts at: 0 1688 1863
Size: 1688 175
BsaBI GATnn'nnATC
Cuts at: 0 137 1863
Size: 137 1726
BsaWI w'CCGG_w
Cuts at: 0 572 1863
Size: 572 1291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 927 957 1142 1172 1863
Size: 927 30 185 30 691
Fragments arranged by size:
927 691 185 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 811 1388 1454 1457 1863
Size: 811 577 66 3 406
Fragments arranged by size:
811 577 406 66 3
BseYI C'CCAG_C
Cuts at: 0 452 456 586 969 1075 1170 1703 1863
Size: 452 4 130 383 106 95 533 160
Fragments arranged by size:
533 452 383 160 130 106 95 4
Bsp1286I G_dGCh'C
Cuts at: 0 296 1001 1199 1863
Size: 296 705 198 664
Fragments arranged by size:
705 664 296 198
BspEI T'CCGG_A
Cuts at: 0 572 1863
Size: 572 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 501 1863
Size: 501 1362
BsrBI CCG'CTC
Cuts at: 0 1551 1863
Size: 1551 312
BsrDI GCAATG_nn'
Cuts at: 0 1785 1863
Size: 1785 78
BssSI C'ACGA_G
Cuts at: 0 550 1863
Size: 550 1313
BstAPI GCAn_nnn'nTGC
Cuts at: 0 862 1285 1288 1863
Size: 862 423 3 575
Fragments arranged by size:
862 575 423 3
BstBI TT'CG_AA
Cuts at: 0 1440 1863
Size: 1440 423
BstEII G'GTnAC_C
Cuts at: 0 1182 1863
Size: 1182 681
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 1863
Size: 321 1542
BstYI r'GATC_y
Cuts at: 0 654 915 1863
Size: 654 261 948
Fragments arranged by size:
948 654 261
Bsu36I CC'TnA_GG
Cuts at: 0 1571 1637 1863
Size: 1571 66 226
Fragments arranged by size:
1571 226 66
BtgI C'CryG_G
Cuts at: 0 267 314 1863
Size: 267 47 1549
Fragments arranged by size:
1549 267 47
BtsI GCAGTG_nn'
Cuts at: 0 1797 1863
Size: 1797 66
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 749 784 1829 1863
Size: 749 35 1045 34
Fragments arranged by size:
1045 749 35 34
EaeI y'GGCC_r
Cuts at: 0 168 1863
Size: 168 1695
EarI CTCTTCn'nnn_
Cuts at: 0 204 634 1134 1863
Size: 204 430 500 729
Fragments arranged by size:
729 500 430 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 871 962 1091 1390 1863
Size: 527 344 91 129 299 473
Fragments arranged by size:
527 473 344 299 129 91
EcoO109I rG'GnC_Cy
Cuts at: 0 43 630 1863
Size: 43 587 1233
Fragments arranged by size:
1233 587 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 532 1863
Size: 532 1331
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 379 411 958 990 1242 1274
Size: 181 32 166 32 547 32 252 32
Cuts at: 1274 1630 1662 1863
Size: 356 32 201
Fragments arranged by size:
547 356 252 201 181 166 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 422 966 1029 1240 1863
Size: 422 544 63 211 623
Fragments arranged by size:
623 544 422 211 63
HindIII A'AGCT_T
Cuts at: 0 90 1863
Size: 90 1773
HpaI GTT'AAC
Cuts at: 0 1029 1863
Size: 1029 834
MfeI C'AATT_G
Cuts at: 0 368 1863
Size: 368 1495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1224 1863
Size: 1224 639
MscI TGG'CCA
Cuts at: 0 170 1863
Size: 170 1693
MslI CAynn'nnrTG
Cuts at: 0 176 253 554 1180 1849 1863
Size: 176 77 301 626 669 14
Fragments arranged by size:
669 626 301 176 77 14
MspA1I CmG'CkG
Cuts at: 0 456 1285 1863
Size: 456 829 578
Fragments arranged by size:
829 578 456
NcoI C'CATG_G
Cuts at: 0 267 314 1863
Size: 267 47 1549
Fragments arranged by size:
1549 267 47
NdeI CA'TA_TG
Cuts at: 0 687 1863
Size: 687 1176
NspI r_CATG'y
Cuts at: 0 252 1863
Size: 252 1611
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 1584 1863
Size: 243 1341 279
Fragments arranged by size:
1341 279 243
PfoI T'CCnGG_A
Cuts at: 0 9 1863
Size: 9 1854
PstI C_TGCA'G
Cuts at: 0 1391 1475 1777 1804 1863
Size: 1391 84 302 27 59
Fragments arranged by size:
1391 302 84 59 27
PvuII CAG'CTG
Cuts at: 0 456 1285 1863
Size: 456 829 578
Fragments arranged by size:
829 578 456
SfcI C'TryA_G
Cuts at: 0 332 1327 1387 1471 1773 1800 1863
Size: 332 995 60 84 302 27 63
Fragments arranged by size:
995 332 302 84 63 60 27
SmlI C'TyrA_G
Cuts at: 0 1145 1863
Size: 1145 718
SpeI A'CTAG_T
Cuts at: 0 538 1863
Size: 538 1325
StyI C'CwwG_G
Cuts at: 0 128 267 314 468 1863
Size: 128 139 47 154 1395
Fragments arranged by size:
1395 154 139 128 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 1250 1863
Size: 85 1165 613
Fragments arranged by size:
1165 613 85
TatI w'GTAC_w
Cuts at: 0 1216 1863
Size: 1216 647
XbaI T'CTAG_A
Cuts at: 0 1831 1863
Size: 1831 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1066 1863
Size: 1066 797
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApoI AvaI AvrII
BamHI BanI BanII BbsI BclI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI
Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI
EarI Eco57MI EcoO109I FalI Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI
PflMI PfoI PstI PvuII SfcI SmlI SpeI StyI
TaqII TatI XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI BaeI
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BsaAI BsaHI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspHI BsrFI BsrGI BssHII BstZ17I
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI
StuI SwaI TstI Tth111I XhoI XmaI XmnI ZraI