Restriction map for
pFN21AA0721
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BbeI BbrPI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CelII DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI SacI SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 199 GTCGAC GT^[AC][GT]AC
AccIII 81 TCCGGA T^CCGGA
AfaI 831 GTAC GT^AC
AlwNI 305 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 71 CCTCAGG CC^T[ACGT]AGG
AvaII 84 GGACC G^G[AT]CC
BalI 807 TGGCCA TGG^CCA
BamHI 192 GGATCC G^GATCC
BanI 995 GGCACC G^G[CT][GA]CC
BbsI 817 GAAGAC GAAGAC(2/6)
BbvCI 825 GCTGAGG CCTCAGC(-5/-2)
BpuAI 817 GAAGAC GAAGAC(2/6)
BsaWI 81 TCCGGA [AT]^CCGG[AT]
BseAI 81 TCCGGA T^CCGGA
BsmFI 223 GTCCC GGGAC(10/14)
BspDI 628 ATCGAT AT^CGAT
BspEI 81 TCCGGA T^CCGGA
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrFI 43 ACCGGC [GA]^CCGG[CT]
BstAPI 304 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 773 TTCGAA TT^CGAA
Bsu36I 71 CCTCAGG CC^T[ACGT]AGG
Cfr10I 43 ACCGGC [GA]^CCGG[CT]
ClaI 628 ATCGAT AT^CGAT
CpoI 83 CGGACCG CG^G[AT]CCG
DsaI 366 CCGTGG C^C[GA][CT]GG
Eco81I 71 CCTCAGG CC^T[ACGT]AGG
FauI 211 CCCGC CCCGC(4/6)
HaeII 1213 AGCGCC [GA]GCGC^[CT]
HgaI 221 GCGTC GACGC(5/10)
HincII 199 GTCGAC GT[CT]^[GA]AC
HindII 199 GTCGAC GT[CT]^[GA]AC
MaeII 647 ACGT A^CGT
MluNI 807 TGGCCA TGG^CCA
MroI 81 TCCGGA T^CCGGA
MscI 807 TGGCCA TGG^CCA
MseI 860 TTAA T^TAA
NspV 773 TTCGAA TT^CGAA
PvuII 787 CAGCTG CAG^CTG
RsaI 831 GTAC GT^AC
RsrII 83 CGGACCG CG^G[AT]CCG
SalI 199 GTCGAC G^TCGAC
SfuI 773 TTCGAA TT^CGAA
SmlI 682 CTTGAG C^T[CT][GA]AG
SphI 701 GCATGC GCATG^C
Tru9I 860 TTAA T^TAA
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
ApaI 587 GGGCCC GGGCC^C
1168 GGGCCC
BcgI 88 CGAGACCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1098 CGACAGAATTGC
BclI 800 TGATCA T^GATCA
836 TGATCA
BglI 313 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
472 GCCATCTCGGC
BpmI 160 CTGGAG CTGGAG(16/14)
619 CTGGAG
BseRI 571 GAGGAG GAGGAG(10/8)
853 GAGGAG
BsgI 248 GTGCAG GTGCAG(16/14)
535 GTGCAG
EcoRI 1093 GAATTC G^AATTC
1181 GAATTC
FbaI 800 TGATCA T^GATCA
836 TGATCA
HinfI 237 GAGTC G^A[ACGT]TC
613 GACTC
MspA1I 787 CAGCTG C[AC]G^C[GT]G
990 CCGCTG
PleI 613 GACTC GAGTC(4/5)
237 GAGTC
SfaNI 274 GATGC GCATC(5/9)
184 GCATC
Tsp45I 454 GTGAC ^GT[GC]AC
792 GTCAC
XbaI 390 TCTAGA T^CTAGA
1089 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI BanII BbvI BcnI BfaI
BmyI BsaI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI StyI TaqI TseI Tsp509I
TspRI TthHB8I XhoII