Restriction map for
pFN21AA0721
(Linear) (Six-base) MAPSORT of: KIAA0721.seq Check: 5014 from: 1 to: 1242
KIAA0721.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 200 1242
Size: 200 1042
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1209 1242
Size: 661 548 33
Fragments arranged by size:
661 548 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 290 1242
Size: 290 952
AlwNI CAG_nnn'CTG
Cuts at: 0 310 1242
Size: 310 932
ApaI G_GGCC'C
Cuts at: 0 591 1172 1242
Size: 591 581 70
Fragments arranged by size:
591 581 70
ApoI r'AATT_y
Cuts at: 0 883 1093 1181 1242
Size: 883 210 88 61
Fragments arranged by size:
883 210 88 61
BamHI G'GATC_C
Cuts at: 0 192 1242
Size: 192 1050
BanI G'GyrC_C
Cuts at: 0 995 1242
Size: 995 247
BanII G_rGCy'C
Cuts at: 0 107 365 591 1172 1242
Size: 107 258 226 581 70
Fragments arranged by size:
581 258 226 107 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 1242
Size: 824 418
BbvCI CC'TCA_GC
Cuts at: 0 826 1242
Size: 826 416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 77 111 1087 1121 1242
Size: 77 34 976 34 121
Fragments arranged by size:
976 121 77 34 34
BclI T'GATC_A
Cuts at: 0 800 836 1242
Size: 800 36 406
Fragments arranged by size:
800 406 36
BglI GCCn_nnn'nGGC
Cuts at: 0 319 478 1242
Size: 319 159 764
Fragments arranged by size:
764 319 159
Bme1580I G_kGCm'C
Cuts at: 0 591 1172 1242
Size: 591 581 70
Fragments arranged by size:
591 581 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 220 1242
Size: 220 1022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 640 1242
Size: 181 459 602
Fragments arranged by size:
602 459 181
Bpu10I CC'TnA_GC
Cuts at: 0 826 1242
Size: 826 416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1242
Size: 703 539
BsaI GGTCTCn'nnnn_
Cuts at: 0 83 157 425 1242
Size: 83 74 268 817
Fragments arranged by size:
817 268 83 74
BsaWI w'CCGG_w
Cuts at: 0 81 1242
Size: 81 1161
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 586 868 1242
Size: 586 282 374
Fragments arranged by size:
586 374 282
BseYI C'CCAG_C
Cuts at: 0 376 785 1242
Size: 376 409 457
Fragments arranged by size:
457 409 376
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 556 1242
Size: 269 287 686
Fragments arranged by size:
686 287 269
Bsp1286I G_dGCh'C
Cuts at: 0 107 365 591 1172 1242
Size: 107 258 226 581 70
Fragments arranged by size:
581 258 226 107 70
BspEI T'CCGG_A
Cuts at: 0 81 1242
Size: 81 1161
BsrBI CCG'CTC
Cuts at: 0 5 1242
Size: 5 1237
BsrFI r'CCGG_y
Cuts at: 0 43 1242
Size: 43 1199
BstAPI GCAn_nnn'nTGC
Cuts at: 0 310 1242
Size: 310 932
BstBI TT'CG_AA
Cuts at: 0 774 1242
Size: 774 468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 343 758 992 1242
Size: 343 415 234 250
Fragments arranged by size:
415 343 250 234
BstYI r'GATC_y
Cuts at: 0 78 192 952 1219 1242
Size: 78 114 760 267 23
Fragments arranged by size:
760 267 114 78 23
Bsu36I CC'TnA_GG
Cuts at: 0 72 1242
Size: 72 1170
BtgI C'CryG_G
Cuts at: 0 366 1242
Size: 366 876
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 529 1242
Size: 529 713
BtsI GCAGTG_nn'
Cuts at: 0 491 1242
Size: 491 751
ClaI AT'CG_AT
Cuts at: 0 629 1242
Size: 629 613
EaeI y'GGCC_r
Cuts at: 0 696 807 960 1242
Size: 696 111 153 282
Fragments arranged by size:
696 282 153 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 640 661 1209 1242
Size: 181 459 21 548 33
Fragments arranged by size:
548 459 181 33 21
EcoO109I rG'GnC_Cy
Cuts at: 0 587 588 668 1168 1169 1242
Size: 587 1 80 500 1 73
Fragments arranged by size:
587 500 80 73 1 1
EcoRI G'AATT_C
Cuts at: 0 1093 1181 1242
Size: 1093 88 61
Fragments arranged by size:
1093 88 61
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 912 1242
Size: 912 330
HaeII r_GCGC'y
Cuts at: 0 1217 1242
Size: 1217 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 1242
Size: 55 32 1155
Fragments arranged by size:
1155 55 32
HincII GTy'rAC
Cuts at: 0 201 1242
Size: 201 1041
MscI TGG'CCA
Cuts at: 0 809 1242
Size: 809 433
MspA1I CmG'CkG
Cuts at: 0 789 992 1242
Size: 789 203 250
Fragments arranged by size:
789 250 203
NspI r_CATG'y
Cuts at: 0 705 720 825 1242
Size: 705 15 105 417
Fragments arranged by size:
705 417 105 15
PasI CC'CwG_GG
Cuts at: 0 584 1242
Size: 584 658
PspOMI G'GGCC_C
Cuts at: 0 587 1168 1242
Size: 587 581 74
Fragments arranged by size:
587 581 74
PvuII CAG'CTG
Cuts at: 0 789 1242
Size: 789 453
RsrII CG'GwC_CG
Cuts at: 0 84 1242
Size: 84 1158
SalI G'TCGA_C
Cuts at: 0 199 1242
Size: 199 1043
SmlI C'TyrA_G
Cuts at: 0 682 1242
Size: 682 560
SphI G_CATG'C
Cuts at: 0 705 1242
Size: 705 537
StyI C'CwwG_G
Cuts at: 0 99 446 1195 1242
Size: 99 347 749 47
Fragments arranged by size:
749 347 99 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 1150 1242
Size: 101 1049 92
Fragments arranged by size:
1049 101 92
XbaI T'CTAG_A
Cuts at: 0 390 1089 1242
Size: 390 699 153
Fragments arranged by size:
699 390 153
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI ApoI BamHI BanI
BanII BbsI BbvCI BcgI BclI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaWI BseRI BseYI BsgI
Bsp1286I BspEI BsrBI BsrFI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI EaeI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII MscI MspA1I NspI
PasI PspOMI PvuII RsrII SalI SmlI SphI StyI
TaqII XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BciVI BfrBI BglII BlpI BmgBI
BmtI BplI BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstEII BstZ17I CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI