(Circular) (Six-base) MAPSORT of: pFN21AA0683.seq Check: 8938 from: 1 to: 7216 pFN21AA0683 7216 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2995 2995 Size: 7216 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5026 278 Size: 53 83 186 936 3490 2468 Fragments arranged by size: 3490 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3630 4559 1937 Size: 1693 929 4594 Fragments arranged by size: 4594 1693 929 Acc65I G'GTAC_C Cuts at: 2707 4537 2707 Size: 1830 5386 AclI AA'CG_TT Cuts at: 1563 5343 5716 1563 Size: 3780 373 3063 Fragments arranged by size: 3780 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 3032 3542 4100 5279 6663 6931 1632 Size: 497 903 510 558 1179 1384 268 1917 Fragments arranged by size: 1917 1384 1179 903 558 510 497 268 AfeI AGC'GCT Cuts at: 2142 2724 3593 4121 2142 Size: 582 869 528 5237 Fragments arranged by size: 5237 869 582 528 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7019 AflIII A'CryG_T Cuts at: 1203 3501 6115 7092 1203 Size: 2298 2614 977 1327 Fragments arranged by size: 2614 2298 1327 977 AgeI A'CCGG_T Cuts at: 6047 6047 Size: 7216 AhdI GACnn_n'nnGTC Cuts at: 2036 5945 2036 Size: 3909 3307 AleI CACnn'nnGTG Cuts at: 1166 4229 1166 Size: 3063 4153 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2531 2759 4271 1184 Size: 1347 228 1512 4129 Fragments arranged by size: 4129 1512 1347 228 AlwNI CAG_nnn'CTG Cuts at: 2956 3010 3871 4312 4959 6531 2956 Size: 54 861 441 647 1572 3641 Fragments arranged by size: 3641 1572 861 647 441 54 ApaI G_GGCC'C Cuts at: 2288 2342 3616 4058 4204 4276 4465 2288 Size: 54 1274 442 146 72 189 5039 Fragments arranged by size: 5039 1274 442 189 146 72 54 ApaLI G'TGCA_C Cuts at: 5273 6429 5273 Size: 1156 6060 ApoI r'AATT_y Cuts at: 1493 3278 4262 4525 4751 4903 7012 1493 Size: 1785 984 263 226 152 2109 1697 Fragments arranged by size: 2109 1785 1697 984 263 226 152 AseI AT'TA_AT Cuts at: 160 1032 5770 160 Size: 872 4738 1606 Fragments arranged by size: 4738 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 AvaI C'yCGr_G Cuts at: 1870 1943 2146 3116 3260 3308 3651 3890 4045 Size: 73 203 970 144 48 343 239 155 Cuts at: 4045 4541 1870 Size: 496 4545 Fragments arranged by size: 4545 970 496 343 239 203 155 144 73 48 BamHI G'GATC_C Cuts at: 3252 4546 4913 3252 Size: 1294 367 5555 Fragments arranged by size: 5555 1294 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2707 2763 2988 3350 3885 Size: 325 217 627 920 56 225 362 535 Cuts at: 3885 4537 5992 618 Size: 652 1455 1842 Fragments arranged by size: 1842 1455 920 652 627 535 362 325 225 217 56 BanII G_rGCy'C Cuts at: 1391 2219 2288 2342 3396 3458 3616 3641 3742 Size: 828 69 54 1054 62 158 25 101 Cuts at: 3742 4058 4204 4276 4465 4535 1391 Size: 316 146 72 189 70 4072 Fragments arranged by size: 4072 1054 828 316 189 158 146 101 72 70 69 62 54 25 BbeI G_GCGC'C Cuts at: 2767 2992 3354 2767 Size: 225 362 6629 Fragments arranged by size: 6629 362 225 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 3707 4167 928 Size: 971 159 1649 460 3977 Fragments arranged by size: 3977 1649 971 460 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2315 2349 2926 2960 3355 3389 5407 Size: 34 378 34 577 34 395 34 2018 Cuts at: 5407 5441 1903 Size: 34 3678 Fragments arranged by size: 3678 2018 577 395 378 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5108 6318 5108 Size: 1210 6006 BclI T'GATC_A Cuts at: 1548 1548 Size: 7216 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2568 2798 2961 Size: 107 122 71 631 605 896 230 163 Cuts at: 2961 3359 3611 3746 3776 4062 5827 136 Size: 398 252 135 30 286 1765 1525 Fragments arranged by size: 1765 1525 896 631 605 398 286 252 230 163 135 122 107 71 30 BglII A'GATC_T Cuts at: 7211 7211 Size: 7216 BlpI GC'TnA_GC Cuts at: 2213 4340 4633 2213 Size: 2127 293 4796 Fragments arranged by size: 4796 2127 293 Bme1580I G_kGCm'C Cuts at: 1790 2288 2342 3250 3368 3616 4058 4204 4276 Size: 498 54 908 118 248 442 146 72 Cuts at: 4276 4465 5277 6433 1790 Size: 189 812 1156 2573 Fragments arranged by size: 2573 1156 908 812 498 442 248 189 146 118 72 54 BmgBI CAC'GTC Cuts at: 1318 3030 3082 3369 3576 4414 1318 Size: 1712 52 287 207 838 4120 Fragments arranged by size: 4120 1712 838 287 207 52 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2840 2884 4947 Size: 416 53 83 399 24 1423 44 2063 Cuts at: 4947 5905 442 Size: 958 1753 Fragments arranged by size: 2063 1753 1423 958 416 399 83 53 44 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7216 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2185 2185 Size: 7216 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 2121 2249 2273 2300 3713 4385 5876 Size: 881 1 128 24 27 1413 672 1491 Cuts at: 5876 1239 Size: 2579 Fragments arranged by size: 2579 1491 1413 881 672 128 27 24 1 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3638 4469 5341 6206 6504 3638 Size: 831 872 865 298 4350 Fragments arranged by size: 4350 872 865 831 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2312 3398 5879 882 Size: 617 813 1086 2481 2219 Fragments arranged by size: 2481 2219 1086 813 617 BsaAI yAC'GTr Cuts at: 493 1204 4300 5007 493 Size: 711 3096 707 2702 Fragments arranged by size: 3096 2702 711 707 BsaBI GATnn'nnATC Cuts at: 7210 7210 Size: 7216 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2764 2939 2989 3214 Size: 53 83 186 936 1231 175 50 225 Cuts at: 3214 3351 3432 3977 5023 5405 6040 275 Size: 137 81 545 1046 382 635 1451 Fragments arranged by size: 1451 1231 1046 936 635 545 382 225 186 175 137 83 81 53 50 BsaWI w'CCGG_w Cuts at: 5649 6047 6321 6468 6879 5649 Size: 398 274 147 411 5986 Fragments arranged by size: 5986 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2246 2276 2408 2438 1178 Size: 30 165 30 843 30 132 30 5956 Fragments arranged by size: 5956 843 165 132 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2270 2588 3710 4016 4318 4487 1186 Size: 988 96 318 1122 306 302 169 3915 Fragments arranged by size: 3915 1122 988 318 306 302 169 96 BseYI C'CCAG_C Cuts at: 2218 2342 2473 2857 2959 3333 3594 4023 4070 Size: 124 131 384 102 374 261 429 47 Cuts at: 4070 6419 6915 2218 Size: 2349 496 2519 Fragments arranged by size: 2519 2349 496 429 384 374 261 131 124 102 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2077 2729 1163 Size: 573 341 652 5650 Fragments arranged by size: 5650 652 573 341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4323 4666 5427 6455 1540 Size: 275 186 2322 343 761 1028 2301 Fragments arranged by size: 2322 2301 1028 761 343 275 186 BsiHKAI G_wGCw'C Cuts at: 5277 5362 6433 5277 Size: 85 1071 6060 Fragments arranged by size: 6060 1071 85 BsmI GAATG_Cn' Cuts at: 4727 4820 4727 Size: 93 7123 BsmBI CGTCTCn'nnnn_ Cuts at: 2459 3020 3958 4330 4515 2459 Size: 561 938 372 185 5160 Fragments arranged by size: 5160 938 561 372 185 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2288 2342 3250 3368 3396 3458 Size: 399 429 69 54 908 118 28 62 Cuts at: 3458 3616 3641 3742 4058 4204 4276 4465 4535 Size: 158 25 101 316 146 72 189 70 Cuts at: 4535 5277 5362 6433 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 908 742 429 399 316 189 158 146 118 101 85 72 70 69 62 54 28 25 BspEI T'CCGG_A Cuts at: 6879 6879 Size: 7216 BspHI T'CATG_A Cuts at: 5105 6835 5105 Size: 1730 5486 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2751 2995 4571 844 Size: 1907 244 1576 3489 Fragments arranged by size: 3489 1907 1576 244 BsrBI CCG'CTC Cuts at: 1394 1823 2995 4006 5103 1394 Size: 429 1172 1011 1097 3507 Fragments arranged by size: 3507 1172 1097 1011 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5711 5885 65 Size: 1183 4463 174 1396 Fragments arranged by size: 4463 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2397 4091 5860 6047 1700 Size: 110 587 1694 1769 187 2869 Fragments arranged by size: 2869 1769 1694 587 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7216 BssHII G'CGCG_C Cuts at: 1925 4355 1925 Size: 2430 4786 BssSI C'ACGA_G Cuts at: 5276 6288 5276 Size: 1012 6204 BstAPI GCAn_nnn'nTGC Cuts at: 794 2956 794 Size: 2162 5054 BstBI TT'CG_AA Cuts at: 6020 6020 Size: 7216 BstEII G'GTnAC_C Cuts at: 1184 4138 1184 Size: 2954 4262 BstXI CCAn_nnnn'nTGG Cuts at: 3166 3166 Size: 7216 BstYI r'GATC_y Cuts at: 1972 2691 3252 4546 4913 5298 5315 6767 6853 Size: 719 561 1294 367 385 17 1452 86 Cuts at: 6853 6865 7211 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1294 719 561 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2838 4195 2838 Size: 1357 5859 BtgI C'CryG_G Cuts at: 513 1065 2004 2242 2674 2793 2973 3384 3972 Size: 552 939 238 432 119 180 411 588 Cuts at: 3972 4086 4227 4242 513 Size: 114 141 15 3487 Fragments arranged by size: 3487 939 588 552 432 411 238 180 141 119 114 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3492 487 Size: 683 56 2266 4211 Fragments arranged by size: 4211 2266 683 56 BtsI GCAGTG_nn' Cuts at: 2412 2429 3049 3973 4732 5526 5546 2412 Size: 17 620 924 759 794 20 4082 Fragments arranged by size: 4082 924 794 759 620 20 17 ClaI AT'CG_AT Cuts at: 1554 4290 1554 Size: 2736 4480 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7181 DraI TTT'AAA Cuts at: 4520 4520 Size: 7216 DraIII CAC_nnn'GTG Cuts at: 3933 3933 Size: 7216 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6223 809 Size: 507 4907 1802 Fragments arranged by size: 4907 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2245 2376 Size: 54 1425 50 275 18 250 165 131 Cuts at: 2376 2532 2970 3156 3672 3747 3777 4089 4093 Size: 156 438 186 516 75 30 312 4 Cuts at: 4093 4239 4320 4663 5552 7181 8 Size: 146 81 343 889 1629 43 Fragments arranged by size: 1629 1425 889 516 438 343 312 275 250 186 165 156 146 131 81 75 54 50 43 30 18 4 EagI C'GGCC_G Cuts at: 1537 1812 4320 4663 1537 Size: 275 2508 343 4090 Fragments arranged by size: 4090 2508 343 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3285 3518 5146 1353 Size: 1932 233 1628 3423 Fragments arranged by size: 3423 1932 1628 233 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2306 2549 3041 4424 5803 6177 Size: 1064 138 717 243 492 1383 1379 374 Cuts at: 6177 6323 387 Size: 146 1280 Fragments arranged by size: 1383 1379 1280 1064 717 492 374 243 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2121 2129 2249 2273 2300 3032 Size: 393 488 1 8 120 24 27 732 Cuts at: 3032 3542 3713 4100 4385 5279 5876 6663 6931 Size: 510 171 387 285 894 597 787 268 Cuts at: 6931 1239 Size: 1524 Fragments arranged by size: 1524 894 787 732 597 510 488 393 387 285 268 171 120 27 24 8 1 EcoNI CCTnn'n_nnAGG Cuts at: 2390 2519 3002 3941 4364 2390 Size: 129 483 939 423 5242 Fragments arranged by size: 5242 939 483 423 129 EcoO109I rG'GnC_Cy Cuts at: 2284 2339 2653 2834 2842 3266 3613 3622 4054 Size: 55 314 181 8 424 347 9 432 Cuts at: 4054 4200 4201 4252 4273 4369 4461 4462 2284 Size: 146 1 51 21 96 92 1 5038 Fragments arranged by size: 5038 432 424 347 314 181 146 96 92 55 51 21 9 8 1 1 EcoRI G'AATT_C Cuts at: 4262 4525 4903 4262 Size: 263 378 6575 Fragments arranged by size: 6575 378 263 FseI GG_CCGG'CC Cuts at: 4095 4095 Size: 7216 FspI TGC'GCA Cuts at: 5722 5722 Size: 7216 HaeII r_GCGC'y Cuts at: 1678 2144 2726 2767 2992 3079 3354 3595 4123 Size: 466 582 41 225 87 275 241 528 Cuts at: 4123 6363 1678 Size: 2240 2531 Fragments arranged by size: 2531 2240 582 528 466 275 241 225 87 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2119 2151 3338 Size: 32 135 32 450 32 65 32 1187 Cuts at: 3338 3370 5857 5889 5931 5963 1373 Size: 32 2487 32 42 32 2626 Fragments arranged by size: 2626 2487 1187 450 135 65 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4222 4560 4806 669 Size: 988 281 2284 338 246 3079 Fragments arranged by size: 3079 2284 988 338 281 246 HindIII A'AGCT_T Cuts at: 748 2909 748 Size: 2161 5055 HpaI GTT'AAC Cuts at: 4222 4806 4222 Size: 584 6632 KasI G'GCGC_C Cuts at: 2763 2988 3350 2763 Size: 225 362 6629 Fragments arranged by size: 6629 362 225 KpnI G_GTAC'C Cuts at: 2711 4541 2711 Size: 1830 5386 MfeI C'AATT_G Cuts at: 4815 4815 Size: 7216 MluI A'CGCG_T Cuts at: 7092 7092 Size: 7216 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3003 6330 6514 1305 Size: 1698 3327 184 2007 Fragments arranged by size: 3327 2007 1698 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 2972 3158 3674 4241 Size: 54 1425 343 546 594 186 516 567 Cuts at: 4241 10 Size: 2985 Fragments arranged by size: 2985 1425 594 567 546 516 343 186 54 MslI CAynn'nnrTG Cuts at: 518 1166 3227 4229 4290 5174 5533 5692 518 Size: 648 2061 1002 61 884 359 159 2042 Fragments arranged by size: 2061 2042 1002 884 648 359 159 61 MspA1I CmG'CkG Cuts at: 1606 2986 3448 4027 4168 4632 5309 6457 6702 Size: 1380 462 579 141 464 677 1148 245 Cuts at: 6702 1606 Size: 2120 Fragments arranged by size: 2120 1380 1148 677 579 464 462 245 141 NaeI GCC'GGC Cuts at: 2399 4093 2399 Size: 1694 5522 NarI GG'CG_CC Cuts at: 2764 2989 3351 2764 Size: 225 362 6629 Fragments arranged by size: 6629 362 225 NcoI C'CATG_G Cuts at: 513 1065 2004 2242 2793 2973 4242 513 Size: 552 939 238 551 180 1269 3487 Fragments arranged by size: 3487 1269 939 552 551 238 180 NdeI CA'TA_TG Cuts at: 387 387 Size: 7216 NgoMIV G'CCGG_C Cuts at: 2397 4091 2397 Size: 1694 5522 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7216 NotI GC'GGCC_GC Cuts at: 4663 4663 Size: 7216 NspI r_CATG'y Cuts at: 1132 1229 3505 4574 6119 1132 Size: 97 2276 1069 1545 2229 Fragments arranged by size: 2276 2229 1545 1069 97 PasI CC'CwG_GG Cuts at: 1348 2731 1348 Size: 1383 5833 PciI A'CATG_T Cuts at: 3501 6115 3501 Size: 2614 4602 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7216 PfoI T'CCnGG_A Cuts at: 2040 2277 2460 3549 3840 2040 Size: 237 183 1089 291 5416 Fragments arranged by size: 5416 1089 291 237 183 PmeI GTTT'AAAC Cuts at: 4520 4520 Size: 7216 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3291 3323 5285 5317 6798 6830 3291 Size: 32 1962 32 1481 32 3677 Fragments arranged by size: 3677 1962 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2653 2842 4369 2653 Size: 189 1527 5500 Fragments arranged by size: 5500 1527 189 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7216 PsiI TTA'TAA Cuts at: 4786 4786 Size: 7216 PspOMI G'GGCC_C Cuts at: 2284 2338 3612 4054 4200 4272 4461 2284 Size: 54 1274 442 146 72 189 5039 Fragments arranged by size: 5039 1274 442 189 146 72 54 PspXI vC'TCGA_Gb Cuts at: 3308 3308 Size: 7216 PstI C_TGCA'G Cuts at: 830 2520 2595 2952 3006 3403 4445 4479 4568 Size: 1690 75 357 54 397 1042 34 89 Cuts at: 4568 830 Size: 3478 Fragments arranged by size: 3478 1690 1042 397 357 89 75 54 34 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7216 PvuII CAG'CTG Cuts at: 3448 4168 3448 Size: 720 6496 RsrII CG'GwC_CG Cuts at: 2785 2785 Size: 7216 SalI G'TCGA_C Cuts at: 1936 4558 1936 Size: 2622 4594 SapI GCTCTTCn'nnn_ Cuts at: 3518 3518 Size: 7216 SbfI CC_TGCA'GG Cuts at: 4568 4568 Size: 7216 ScaI AGT'ACT Cuts at: 5464 7176 5464 Size: 1712 5504 SexAI A'CCwGG_T Cuts at: 2865 2865 Size: 7216 SfcI C'TryA_G Cuts at: 826 1045 2516 2591 2948 3002 3399 3631 3814 Size: 219 1471 75 357 54 397 232 183 Cuts at: 3814 4441 4475 4564 5699 6380 6571 826 Size: 627 34 89 1135 681 191 1471 Fragments arranged by size: 1471 1471 1135 681 627 397 357 232 219 191 183 89 75 54 34 SfiI GGCCn_nnn'nGGCC Cuts at: 3776 3776 Size: 7216 SfoI GGC'GCC Cuts at: 2765 2990 3352 2765 Size: 225 362 6629 Fragments arranged by size: 6629 362 225 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7216 SmaI CCC'GGG Cuts at: 1872 4543 1872 Size: 2671 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3308 3617 4484 5320 6221 6483 Size: 197 926 1365 309 867 836 901 262 Cuts at: 6483 820 Size: 1553 Fragments arranged by size: 1553 1365 926 901 867 836 309 262 197 SnaBI TAC'GTA Cuts at: 493 5007 493 Size: 4514 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7216 SphI G_CATG'C Cuts at: 1132 4574 1132 Size: 3442 3774 SspI AAT'ATT Cuts at: 5 52 5140 5 Size: 47 5088 2081 Fragments arranged by size: 5088 2081 47 StuI AGG'CCT Cuts at: 1517 3880 4132 1517 Size: 2363 252 4601 Fragments arranged by size: 4601 2363 252 StyI C'CwwG_G Cuts at: 513 1065 2004 2242 2614 2656 2793 2973 3321 Size: 552 939 238 372 42 137 180 348 Cuts at: 3321 3675 4035 4242 4655 513 Size: 354 360 207 413 3074 Fragments arranged by size: 3074 939 552 413 372 360 354 348 238 207 180 137 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2744 3873 5243 5260 5413 5598 6043 Size: 1255 1164 1129 1370 17 153 185 445 Cuts at: 6043 325 Size: 1498 Fragments arranged by size: 1498 1370 1255 1164 1129 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5462 7174 96 Size: 274 80 33 51 1443 3485 1712 138 Fragments arranged by size: 3485 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2246 2278 2408 2440 2653 2685 4253 4285 7176 Size: 32 130 32 213 32 1568 32 2891 Cuts at: 7176 7208 2246 Size: 32 2254 Fragments arranged by size: 2891 2254 1568 213 130 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3574 1316 Size: 2258 4958 XbaI T'CTAG_A Cuts at: 4552 4552 Size: 7216 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2275 4234 2275 Size: 1959 5257 XhoI C'TCGA_G Cuts at: 1943 3308 1943 Size: 1365 5851 XmaI C'CCGG_G Cuts at: 1870 4541 1870 Size: 2671 4545 XmnI GAAnn'nnTTC Cuts at: 2049 2555 5345 2049 Size: 506 2790 3920 Fragments arranged by size: 3920 2790 506 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5024 276 Size: 53 83 186 936 3490 2468 Fragments arranged by size: 3490 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoRV FalI FspAI NruI NsiI PacI PmlI PsrI SacI SacII SanDI SrfI SwaI