(Circular) (Six-base) MAPSORT of: pFN21AA0625.seq Check: 8507 from: 1 to: 12736 pFN21AA0625 12736 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3896 10546 278 Size: 53 83 186 936 2360 6650 2468 Fragments arranged by size: 6650 2468 2360 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2274 3072 7942 9117 10079 1937 Size: 337 798 4870 1175 962 4594 Fragments arranged by size: 4870 4594 1175 962 798 337 Acc65I G'GTAC_C Cuts at: 10057 10057 Size: 12736 AclI AA'CG_TT Cuts at: 1563 10863 11236 1563 Size: 9300 373 3063 Fragments arranged by size: 9300 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3167 3339 4128 4704 5286 5409 5535 6032 Size: 1535 172 789 576 582 123 126 497 Cuts at: 6032 6369 6534 6732 6840 8512 9596 9650 10799 Size: 337 165 198 108 1672 1084 54 1149 Cuts at: 10799 12183 12451 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1672 1535 1384 1149 1084 789 582 576 497 337 268 198 172 165 126 123 108 54 AfeI AGC'GCT Cuts at: 2063 2063 Size: 12736 AflII C'TTAA_G Cuts at: 820 1017 3266 7795 820 Size: 197 2249 4529 5761 Fragments arranged by size: 5761 4529 2249 197 AflIII A'CryG_T Cuts at: 1203 2012 2386 2685 3147 4235 4381 9167 9854 Size: 809 374 299 462 1088 146 4786 687 Cuts at: 9854 11635 12612 1203 Size: 1781 977 1327 Fragments arranged by size: 4786 1781 1327 1088 977 809 687 462 374 299 146 AgeI A'CCGG_T Cuts at: 11567 11567 Size: 12736 AhdI GACnn_n'nnGTC Cuts at: 11465 11465 Size: 12736 AleI CACnn'nnGTG Cuts at: 1166 3818 1166 Size: 2652 10084 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 8397 9792 1184 Size: 7213 1395 4128 Fragments arranged by size: 7213 4128 1395 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9074 9106 9074 Size: 32 12704 AlwNI CAG_nnn'CTG Cuts at: 3350 5060 6004 6176 6302 9754 10479 12051 3350 Size: 1710 944 172 126 3452 725 1572 4035 Fragments arranged by size: 4035 3452 1710 1572 944 725 172 126 ApaI G_GGCC'C Cuts at: 7167 7167 Size: 12736 ApaLI G'TGCA_C Cuts at: 8022 10793 11949 8022 Size: 2771 1156 8809 Fragments arranged by size: 8809 2771 1156 ApoI r'AATT_y Cuts at: 1493 2090 3371 3709 3791 4444 4667 5456 5822 Size: 597 1281 338 82 653 223 789 366 Cuts at: 5822 6151 6964 7315 7340 7532 8072 8128 8258 Size: 329 813 351 25 192 540 56 130 Cuts at: 8258 10045 10271 10423 12532 1493 Size: 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 1281 813 789 653 597 540 366 351 338 329 226 223 192 152 130 82 56 25 AseI AT'TA_AT Cuts at: 160 1032 5987 8160 11290 160 Size: 872 4955 2173 3130 1606 Fragments arranged by size: 4955 3130 2173 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12736 AvaI C'yCGr_G Cuts at: 1870 1943 9685 9842 10061 1870 Size: 73 7742 157 219 4545 Fragments arranged by size: 7742 4545 219 157 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4479 4512 4479 Size: 33 12703 BamHI G'GATC_C Cuts at: 2854 2932 9673 10066 10433 2854 Size: 78 6741 393 367 5157 Fragments arranged by size: 6741 5157 393 367 78 BanI G'GyrC_C Cuts at: 618 943 1160 1787 9338 10057 11512 618 Size: 325 217 627 7551 719 1455 1842 Fragments arranged by size: 7551 1842 1455 719 627 325 217 BanII G_rGCy'C Cuts at: 1391 2783 7167 8302 9692 9891 10055 1391 Size: 1392 4384 1135 1390 199 164 4072 Fragments arranged by size: 4384 4072 1392 1390 1135 199 164 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2115 3896 5664 6100 6396 9065 928 Size: 971 216 1781 1768 436 296 2669 4599 Fragments arranged by size: 4599 2669 1781 1768 971 436 296 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3049 3083 8286 8320 10927 10961 1903 Size: 34 1112 34 5203 34 2607 34 3678 Fragments arranged by size: 5203 3678 2607 1112 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6888 10628 11838 6888 Size: 3740 1210 7786 Fragments arranged by size: 7786 3740 1210 BclI T'GATC_A Cuts at: 1548 3975 5938 8739 1548 Size: 2427 1963 2801 5545 Fragments arranged by size: 5545 2801 2427 1963 BfrBI ATG'CAT Cuts at: 3910 6252 8245 8785 9131 3910 Size: 2342 1993 540 346 7515 Fragments arranged by size: 7515 2342 1993 540 346 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 11347 136 Size: 107 122 71 631 605 9675 1525 Fragments arranged by size: 9675 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4155 4747 5578 7498 12731 4155 Size: 592 831 1920 5233 4160 Fragments arranged by size: 5233 4160 1920 831 592 BlpI GC'TnA_GC Cuts at: 10153 10153 Size: 12736 Bme1580I G_kGCm'C Cuts at: 1790 7167 8026 10797 11953 1790 Size: 5377 859 2771 1156 2573 Fragments arranged by size: 5377 2771 2573 1156 859 BmgBI CAC'GTC Cuts at: 1318 6422 7545 1318 Size: 5104 1123 6509 Fragments arranged by size: 6509 5104 1123 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2319 7375 8150 Size: 416 53 83 399 24 902 5056 775 Cuts at: 8150 10467 11425 442 Size: 2317 958 1753 Fragments arranged by size: 5056 2317 1753 958 902 775 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12736 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6478 6478 Size: 12736 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6279 6448 7047 9723 9813 10016 11396 1239 Size: 5040 169 599 2676 90 203 1380 2579 Fragments arranged by size: 5040 2676 2579 1380 599 203 169 90 Bpu10I CC'TnA_GC Cuts at: 5838 8628 9755 5838 Size: 2790 1127 8819 Fragments arranged by size: 8819 2790 1127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2768 4896 5114 5147 7177 7578 8513 9383 10861 Size: 2128 218 33 2030 401 935 870 1478 Cuts at: 10861 11726 12024 2768 Size: 865 298 3480 Fragments arranged by size: 3480 2128 2030 1478 935 870 865 401 298 218 33 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4878 6297 6775 9944 11399 882 Size: 617 3379 1419 478 3169 1455 2219 Fragments arranged by size: 3379 3169 2219 1455 1419 617 478 BsaAI yAC'GTr Cuts at: 493 1204 5636 5936 9168 9855 10527 493 Size: 711 4432 300 3232 687 672 2702 Fragments arranged by size: 4432 3232 2702 711 687 672 300 BsaBI GATnn'nnATC Cuts at: 2460 8869 12730 2460 Size: 6409 3861 2466 Fragments arranged by size: 6409 3861 2466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3893 8410 10543 10925 Size: 53 83 186 936 2360 4517 2133 382 Cuts at: 10925 11560 275 Size: 635 1451 Fragments arranged by size: 4517 2360 2133 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2084 3028 11169 11567 11841 11988 12399 2084 Size: 944 8141 398 274 147 411 2421 Fragments arranged by size: 8141 2421 944 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5608 5638 7485 7515 7967 Size: 30 165 30 4205 30 1847 30 452 Cuts at: 7967 7997 8566 8596 9415 9445 1178 Size: 30 569 30 819 30 4469 Fragments arranged by size: 4469 4205 1847 819 569 452 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4793 5985 6750 7922 9899 1186 Size: 3607 1192 765 1172 1977 4023 Fragments arranged by size: 4023 3607 1977 1192 1172 765 BseYI C'CCAG_C Cuts at: 3450 4058 5904 9476 9693 11939 12435 3450 Size: 608 1846 3572 217 2246 496 3751 Fragments arranged by size: 3751 3572 2246 1846 608 496 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3632 8446 1163 Size: 573 1896 4814 5453 Fragments arranged by size: 5453 4814 1896 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 10186 10947 11975 1540 Size: 275 186 8185 761 1028 2301 Fragments arranged by size: 8185 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 8026 8302 8474 9891 10797 10882 11953 2012 Size: 6014 276 172 1417 906 85 1071 2795 Fragments arranged by size: 6014 2795 1417 1071 906 276 172 85 BsmI GAATG_Cn' Cuts at: 3574 7969 9131 10247 10340 3574 Size: 4395 1162 1116 93 5970 Fragments arranged by size: 5970 4395 1162 1116 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2213 6876 2213 Size: 4663 8073 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2783 7167 8026 8302 8474 9692 Size: 399 222 771 4384 859 276 172 1218 Cuts at: 9692 9891 10055 10797 10882 11953 1391 Size: 199 164 742 85 1071 2174 Fragments arranged by size: 4384 2174 1218 1071 859 771 742 399 276 222 199 172 164 85 BspEI T'CCGG_A Cuts at: 12399 12399 Size: 12736 BspHI T'CATG_A Cuts at: 8052 9601 10625 12355 8052 Size: 1549 1024 1730 8433 Fragments arranged by size: 8433 1730 1549 1024 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 10091 844 Size: 9247 3489 BsrBI CCG'CTC Cuts at: 1394 1823 10623 1394 Size: 429 8800 3507 Fragments arranged by size: 8800 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3461 3558 3569 6979 11231 11405 65 Size: 1183 2213 97 11 3410 4252 174 1396 Fragments arranged by size: 4252 3410 2213 1396 1183 174 97 11 BsrFI r'CCGG_y Cuts at: 1700 1810 11380 11567 1700 Size: 110 9570 187 2869 Fragments arranged by size: 9570 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3595 5372 5690 6197 7748 96 Size: 3499 1777 318 507 1551 5084 Fragments arranged by size: 5084 3499 1777 1551 507 318 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12736 BssSI C'ACGA_G Cuts at: 8015 9243 10796 11808 8015 Size: 1228 1553 1012 8943 Fragments arranged by size: 8943 1553 1228 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3580 794 Size: 2786 9950 BstBI TT'CG_AA Cuts at: 9022 11540 9022 Size: 2518 10218 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 12736 BstXI CCAn_nnnn'nTGG Cuts at: 2500 9701 2500 Size: 7201 5535 BstYI r'GATC_y Cuts at: 1972 2469 2854 2906 2932 2985 3262 3823 4155 Size: 497 385 52 26 53 277 561 332 Cuts at: 4155 4747 5518 5578 7498 8623 9673 10066 10433 Size: 592 771 60 1920 1125 1050 393 367 Cuts at: 10433 10818 10835 12287 12373 12385 12731 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1920 1452 1125 1050 771 592 561 497 393 385 385 367 346 332 277 86 60 53 52 26 17 12 BstZ17I GTA'TAC Cuts at: 3073 3073 Size: 12736 BtgI C'CryG_G Cuts at: 513 1065 2760 5032 5435 8506 513 Size: 552 1695 2272 403 3071 4743 Fragments arranged by size: 4743 3071 2272 1695 552 403 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4941 8255 8579 487 Size: 683 56 3715 3314 324 4644 Fragments arranged by size: 4644 3715 3314 683 324 56 BtsI GCAGTG_nn' Cuts at: 3495 6475 9387 10252 11046 11066 3495 Size: 2980 2912 865 794 20 5165 Fragments arranged by size: 5165 2980 2912 865 794 20 ClaI AT'CG_AT Cuts at: 1554 2810 1554 Size: 1256 11480 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12701 DraI TTT'AAA Cuts at: 2596 4305 4744 5599 7617 10040 2596 Size: 1709 439 855 2018 2423 5292 Fragments arranged by size: 5292 2423 2018 1709 855 439 DraIII CAC_nnn'GTG Cuts at: 6648 6648 Size: 12736 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 11743 809 Size: 507 10427 1802 Fragments arranged by size: 10427 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2864 7608 8879 Size: 54 1425 50 275 18 1034 4744 1271 Cuts at: 8879 10183 11072 12701 8 Size: 1304 889 1629 43 Fragments arranged by size: 4744 1629 1425 1304 1271 1034 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 10183 1537 Size: 275 8371 4090 Fragments arranged by size: 8371 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2349 4165 4400 7458 8712 8807 9266 9626 Size: 996 1816 235 3058 1254 95 459 360 Cuts at: 9626 9876 10666 1353 Size: 250 790 3423 Fragments arranged by size: 3423 3058 1816 1254 996 790 459 360 250 235 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 11323 11697 11843 387 Size: 1064 138 9734 374 146 1280 Fragments arranged by size: 9734 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 8300 9889 8300 Size: 1589 11147 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3167 3339 4128 4704 5286 5409 5535 Size: 393 1535 172 789 576 582 123 126 Cuts at: 5535 6032 6279 6369 6448 6534 6732 6840 7047 Size: 497 247 90 79 86 198 108 207 Cuts at: 7047 8512 9596 9650 9723 9813 10016 10799 11396 Size: 1465 1084 54 73 90 203 783 597 Cuts at: 11396 12183 12451 1239 Size: 787 268 1524 Fragments arranged by size: 1535 1524 1465 1084 789 787 783 597 582 576 497 393 268 247 207 203 198 172 126 123 108 90 90 86 79 73 54 EcoNI CCTnn'n_nnAGG Cuts at: 2654 5873 7902 2654 Size: 3219 2029 7488 Fragments arranged by size: 7488 3219 2029 EcoO109I rG'GnC_Cy Cuts at: 4831 5879 7164 9282 9580 9616 9751 4831 Size: 1048 1285 2118 298 36 135 7816 Fragments arranged by size: 7816 2118 1285 1048 298 135 36 EcoRI G'AATT_C Cuts at: 6151 6964 8072 10045 10423 6151 Size: 813 1108 1973 378 8464 Fragments arranged by size: 8464 1973 1108 813 378 EcoRV GAT'ATC Cuts at: 3558 3558 Size: 12736 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2600 3709 6683 2600 Size: 1109 2974 8653 Fragments arranged by size: 8653 2974 1109 FspI TGC'GCA Cuts at: 11242 11242 Size: 12736 HaeII r_GCGC'y Cuts at: 1678 2065 11883 1678 Size: 387 9818 2531 Fragments arranged by size: 9818 2531 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2881 2898 2913 2930 3509 Size: 32 135 32 1309 17 15 17 579 Cuts at: 3509 3541 3610 3642 4715 4747 5122 5154 5194 Size: 32 69 32 1073 32 375 32 40 Cuts at: 5194 5226 5369 5401 5543 5575 5608 5640 7967 Size: 32 143 32 142 32 33 32 2327 Cuts at: 7967 7999 8566 8598 8924 8956 9216 9248 11377 Size: 32 567 32 326 32 260 32 2129 Cuts at: 11377 11409 11451 11483 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2327 2129 1309 1073 579 567 375 326 260 143 142 135 69 42 40 33 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2392 2924 3732 4245 10080 10326 Size: 988 281 454 532 808 513 5835 246 Cuts at: 10326 669 Size: 3079 Fragments arranged by size: 5835 3079 988 808 532 513 454 281 246 HindIII A'AGCT_T Cuts at: 748 2330 3485 3921 5678 8987 10028 748 Size: 1582 1155 436 1757 3309 1041 3456 Fragments arranged by size: 3456 3309 1757 1582 1155 1041 436 HpaI GTT'AAC Cuts at: 2392 10326 2392 Size: 7934 4802 KpnI G_GTAC'C Cuts at: 10061 10061 Size: 12736 MfeI C'AATT_G Cuts at: 2434 4651 9198 10335 2434 Size: 2217 4547 1137 4835 Fragments arranged by size: 4835 4547 2217 1137 MluI A'CGCG_T Cuts at: 12612 12612 Size: 12736 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 2399 3013 4999 5935 6437 6606 7029 Size: 792 302 614 1986 936 502 169 423 Cuts at: 7029 7679 8666 8890 11850 12034 1305 Size: 650 987 224 2960 184 2007 Fragments arranged by size: 2960 2007 1986 987 936 792 650 614 502 423 302 224 184 169 MscI TGG'CCA Cuts at: 10 64 1489 1832 2866 8881 10 Size: 54 1425 343 1034 6015 3865 Fragments arranged by size: 6015 3865 1425 1034 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2385 2493 2688 3229 3805 3818 4677 Size: 648 1219 108 195 541 576 13 859 Cuts at: 4677 5745 7221 7288 8654 10694 11053 11212 518 Size: 1068 1476 67 1366 2040 359 159 2042 Fragments arranged by size: 2042 2040 1476 1366 1219 1068 859 648 576 541 359 195 159 108 67 13 MspA1I CmG'CkG Cuts at: 1606 3616 4963 5855 5908 8039 8275 8532 9313 Size: 2010 1347 892 53 2131 236 257 781 Cuts at: 9313 9776 9833 10152 10829 11977 12222 1606 Size: 463 57 319 677 1148 245 2120 Fragments arranged by size: 2131 2120 2010 1347 1148 892 781 677 463 319 257 245 236 57 53 NcoI C'CATG_G Cuts at: 513 1065 2760 513 Size: 552 1695 10489 Fragments arranged by size: 10489 1695 552 NdeI CA'TA_TG Cuts at: 387 5893 7840 8795 387 Size: 5506 1947 955 4328 Fragments arranged by size: 5506 4328 1947 955 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12736 NotI GC'GGCC_GC Cuts at: 10183 10183 Size: 12736 NsiI A_TGCA'T Cuts at: 3912 6254 8247 8787 9133 3912 Size: 2342 1993 540 346 7515 Fragments arranged by size: 7515 2342 1993 540 346 NspI r_CATG'y Cuts at: 1132 1229 2016 2689 2734 3151 3208 3840 4385 Size: 97 787 673 45 417 57 632 545 Cuts at: 4385 5153 6256 10094 11639 1132 Size: 768 1103 3838 1545 2229 Fragments arranged by size: 3838 2229 1545 1103 787 768 673 632 545 417 97 57 45 PasI CC'CwG_GG Cuts at: 1348 9463 1348 Size: 8115 4621 PciI A'CATG_T Cuts at: 2012 2685 3147 4381 11635 2012 Size: 673 462 1234 7254 3113 Fragments arranged by size: 7254 3113 1234 673 462 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 12736 PfoI T'CCnGG_A Cuts at: 9700 9700 Size: 12736 PmeI GTTT'AAAC Cuts at: 10040 10040 Size: 12736 PmlI CAC'GTG Cuts at: 9855 9855 Size: 12736 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10805 10837 12318 12350 10805 Size: 32 1481 32 11191 Fragments arranged by size: 11191 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5879 9282 9580 9616 9751 5879 Size: 3403 298 36 135 8864 Fragments arranged by size: 8864 3403 298 135 36 PshAI GACnn'nnGTC Cuts at: 1613 4842 1613 Size: 3229 9507 PsiI TTA'TAA Cuts at: 3861 6192 6555 8823 10306 3861 Size: 2331 363 2268 1483 6291 Fragments arranged by size: 6291 2331 2268 1483 363 PspOMI G'GGCC_C Cuts at: 7163 7163 Size: 12736 PspXI vC'TCGA_Gb Cuts at: 9685 9685 Size: 12736 PstI C_TGCA'G Cuts at: 830 8708 9414 10088 830 Size: 7878 706 674 3478 Fragments arranged by size: 7878 3478 706 674 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12736 PvuII CAG'CTG Cuts at: 3616 4963 5855 8039 8275 8532 9313 9833 3616 Size: 1347 892 2184 236 257 781 520 6519 Fragments arranged by size: 6519 2184 1347 892 781 520 257 236 SacI G_AGCT'C Cuts at: 8302 9891 8302 Size: 1589 11147 SalI G'TCGA_C Cuts at: 1936 10078 1936 Size: 8142 4594 SbfI CC_TGCA'GG Cuts at: 10088 10088 Size: 12736 ScaI AGT'ACT Cuts at: 4411 6814 10984 12696 4411 Size: 2403 4170 1712 4451 Fragments arranged by size: 4451 4170 2403 1712 SfcI C'TryA_G Cuts at: 826 1045 3993 5343 5805 6336 8704 8759 9367 Size: 219 2948 1350 462 531 2368 55 608 Cuts at: 9367 9410 9780 10084 11219 11900 12091 826 Size: 43 370 304 1135 681 191 1471 Fragments arranged by size: 2948 2368 1471 1350 1135 681 608 531 462 370 304 219 191 55 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12736 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12736 SmaI CCC'GGG Cuts at: 1872 10063 1872 Size: 8191 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2747 3266 4911 5093 5126 7192 Size: 197 926 804 519 1645 182 33 2066 Cuts at: 7192 7593 7795 8492 9398 9685 10840 11741 12003 Size: 401 202 697 906 287 1155 901 262 Cuts at: 12003 820 Size: 1553 Fragments arranged by size: 2066 1645 1553 1155 926 906 901 804 697 519 401 287 262 202 197 182 33 SnaBI TAC'GTA Cuts at: 493 10527 493 Size: 10034 2702 SpeI A'CTAG_T Cuts at: 152 3734 6035 152 Size: 3582 2301 6853 Fragments arranged by size: 6853 3582 2301 SphI G_CATG'C Cuts at: 1132 6256 10094 1132 Size: 5124 3838 3774 Fragments arranged by size: 5124 3838 3774 SspI AAT'ATT Cuts at: 5 52 7046 9663 10660 5 Size: 47 6994 2617 997 2081 Fragments arranged by size: 6994 2617 2081 997 47 StuI AGG'CCT Cuts at: 1517 5530 1517 Size: 4013 8723 StyI C'CwwG_G Cuts at: 513 1065 2760 2902 9190 9549 10175 513 Size: 552 1695 142 6288 359 626 3074 Fragments arranged by size: 6288 3074 1695 626 552 359 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3266 3448 6389 10763 10780 10933 11118 Size: 1255 1686 182 2941 4374 17 153 185 Cuts at: 11118 11563 325 Size: 445 1498 Fragments arranged by size: 4374 2941 1686 1498 1255 445 185 182 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3178 3312 3595 Size: 274 80 33 51 1443 1201 134 283 Cuts at: 3595 4127 4181 4409 5372 5684 5690 6113 6197 Size: 532 54 228 963 312 6 423 84 Cuts at: 6197 6654 6812 7748 8775 9252 10982 12694 96 Size: 457 158 936 1027 477 1730 1712 138 Fragments arranged by size: 1730 1712 1443 1201 1027 963 936 532 477 457 423 312 283 274 228 158 138 134 84 80 54 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9415 9447 12696 12728 9415 Size: 32 3249 32 9423 Fragments arranged by size: 9423 3249 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3362 8142 1316 Size: 2046 4780 5910 Fragments arranged by size: 5910 4780 2046 XbaI T'CTAG_A Cuts at: 3896 8263 10072 3896 Size: 4367 1809 6560 Fragments arranged by size: 6560 4367 1809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4055 5015 7687 9197 4055 Size: 960 2672 1510 7594 Fragments arranged by size: 7594 2672 1510 960 XhoI C'TCGA_G Cuts at: 1943 9685 1943 Size: 7742 4994 XmaI C'CCGG_G Cuts at: 1870 10061 1870 Size: 8191 4545 XmnI GAAnn'nnTTC Cuts at: 2502 3375 5617 6097 6570 8859 10865 2502 Size: 873 2242 480 473 2289 2006 4373 Fragments arranged by size: 4373 2289 2242 2006 873 480 473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3894 10544 276 Size: 53 83 186 936 2360 6650 2468 Fragments arranged by size: 6650 2468 2360 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BbvCI BsiWI Bsu36I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI