(Circular) (Six-base) MAPSORT of: pFN21AA0589.seq Check: 6918 from: 1 to: 6709 pFN21AA0589 6709 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3727 3880 3727 Size: 153 6556 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3635 4519 278 Size: 53 83 186 936 2099 884 2468 Fragments arranged by size: 2468 2099 936 884 186 83 53 AccI GT'mk_AC Cuts at: 1937 4052 1937 Size: 2115 4594 Acc65I G'GTAC_C Cuts at: 2017 4030 2017 Size: 2013 4696 AclI AA'CG_TT Cuts at: 1563 4836 5209 1563 Size: 3273 373 3063 Fragments arranged by size: 3273 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 2872 3374 4772 6156 6424 1632 Size: 629 611 502 1398 1384 268 1917 Fragments arranged by size: 1917 1398 1384 629 611 502 268 AfeI AGC'GCT Cuts at: 2038 3305 2038 Size: 1267 5442 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6512 AflIII A'CryG_T Cuts at: 1203 5608 6585 1203 Size: 4405 977 1327 Fragments arranged by size: 4405 1327 977 AgeI A'CCGG_T Cuts at: 5540 5540 Size: 6709 AhdI GACnn_n'nnGTC Cuts at: 3271 5438 3271 Size: 2167 4542 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6709 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6709 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2576 2608 2576 Size: 32 6677 AlwNI CAG_nnn'CTG Cuts at: 2605 4452 6024 2605 Size: 1847 1572 3290 Fragments arranged by size: 3290 1847 1572 ApaI G_GGCC'C Cuts at: 2159 3417 3487 2159 Size: 1258 70 5381 Fragments arranged by size: 5381 1258 70 ApaLI G'TGCA_C Cuts at: 2972 4766 5922 2972 Size: 1794 1156 3759 Fragments arranged by size: 3759 1794 1156 ApoI r'AATT_y Cuts at: 1493 4018 4244 4396 6505 1493 Size: 2525 226 152 2109 1697 Fragments arranged by size: 2525 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2788 2788 Size: 6709 AseI AT'TA_AT Cuts at: 160 1032 5263 160 Size: 872 4231 1606 Fragments arranged by size: 4231 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6709 AvaI C'yCGr_G Cuts at: 1870 1943 2512 2858 4034 1870 Size: 73 569 346 1176 4545 Fragments arranged by size: 4545 1176 569 346 73 BamHI G'GATC_C Cuts at: 2451 3141 4039 4406 2451 Size: 690 898 367 4754 Fragments arranged by size: 4754 898 690 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2246 2516 3014 3095 Size: 325 217 627 230 229 270 498 81 Cuts at: 3095 3812 4030 5485 618 Size: 717 218 1455 1842 Fragments arranged by size: 1842 1455 717 627 498 325 270 230 229 218 217 81 BanII G_rGCy'C Cuts at: 1391 2159 2291 2445 2774 2868 3417 3487 4028 Size: 768 132 154 329 94 549 70 541 Cuts at: 4028 1391 Size: 4072 Fragments arranged by size: 4072 768 549 541 329 154 132 94 70 BbeI G_GCGC'C Cuts at: 2520 3018 3099 2520 Size: 498 81 6130 Fragments arranged by size: 6130 498 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2228 928 Size: 971 329 5409 Fragments arranged by size: 5409 971 329 BbvCI CC'TCA_GC Cuts at: 2040 2040 Size: 6709 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4900 4934 1903 Size: 34 2963 34 3678 Fragments arranged by size: 3678 2963 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4601 5811 4601 Size: 1210 5499 BclI T'GATC_A Cuts at: 1548 1548 Size: 6709 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2863 3431 3848 Size: 107 122 71 631 605 1191 568 417 Cuts at: 3848 3881 3983 5320 136 Size: 33 102 1337 1525 Fragments arranged by size: 1525 1337 1191 631 605 568 417 122 107 102 71 33 BglII A'GATC_T Cuts at: 6704 6704 Size: 6709 BlpI GC'TnA_GC Cuts at: 4126 4126 Size: 6709 Bme1580I G_kGCm'C Cuts at: 1790 2058 2159 2976 3417 3487 4770 5926 1790 Size: 268 101 817 441 70 1283 1156 2573 Fragments arranged by size: 2573 1283 1156 817 441 268 101 70 BmgBI CAC'GTC Cuts at: 1318 2308 1318 Size: 990 5719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3460 4440 5398 Size: 416 53 83 399 24 2043 980 958 Cuts at: 5398 442 Size: 1753 Fragments arranged by size: 2043 1753 980 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3398 1056 Size: 2342 4367 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 3251 3524 5369 1239 Size: 794 1218 273 1845 2579 Fragments arranged by size: 2579 1845 1218 794 273 Bpu10I CC'TnA_GC Cuts at: 2040 2285 3612 2040 Size: 245 1327 5137 Fragments arranged by size: 5137 1327 245 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2738 2750 4834 5699 5997 2738 Size: 12 2084 865 298 3450 Fragments arranged by size: 3450 2084 865 298 12 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5372 882 Size: 617 3873 2219 Fragments arranged by size: 3873 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2329 4500 493 Size: 711 1125 2171 2702 Fragments arranged by size: 2702 2171 1125 711 BsaBI GATnn'nnATC Cuts at: 6703 6703 Size: 6709 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2517 2877 3015 3096 Size: 53 83 186 936 984 360 138 81 Cuts at: 3096 3632 4516 4898 5533 275 Size: 536 884 382 635 1451 Fragments arranged by size: 1451 984 936 884 635 536 382 360 186 138 83 81 53 BsaWI w'CCGG_w Cuts at: 2020 5142 5540 5814 5961 6372 2020 Size: 3122 398 274 147 411 2357 Fragments arranged by size: 3122 2357 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2054 2084 2273 2303 2459 Size: 30 165 30 651 30 189 30 156 Cuts at: 2459 2489 2576 2606 3452 3482 3734 3764 1178 Size: 30 87 30 846 30 252 30 4123 Fragments arranged by size: 4123 846 651 252 189 165 156 87 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2221 3337 3473 3710 3794 3875 3908 3952 Size: 1035 1116 136 237 84 81 33 44 Cuts at: 3952 1186 Size: 3943 Fragments arranged by size: 3943 1116 1035 237 136 84 81 44 33 BseYI C'CCAG_C Cuts at: 2257 2351 2965 3287 3409 3942 5912 6408 2257 Size: 94 614 322 122 533 1970 496 2558 Fragments arranged by size: 2558 1970 614 533 496 322 122 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2702 3164 3520 3755 3772 1163 Size: 573 966 462 356 235 17 4100 Fragments arranged by size: 4100 966 573 462 356 235 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3810 4159 4920 5948 1540 Size: 275 186 1159 650 349 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 650 349 275 186 BsiHKAI G_wGCw'C Cuts at: 2291 2313 2445 2976 3026 3237 4770 4855 5926 Size: 22 132 531 50 211 1533 85 1071 Cuts at: 5926 2291 Size: 3074 Fragments arranged by size: 3074 1533 1071 531 211 132 85 50 22 BsmI GAATG_Cn' Cuts at: 4220 4313 4220 Size: 93 6616 BsmBI CGTCTCn'nnnn_ Cuts at: 3623 3623 Size: 6709 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2159 2291 2313 2445 2774 2868 Size: 399 268 101 132 22 132 329 94 Cuts at: 2868 2976 3026 3237 3417 3487 4028 4770 4855 Size: 108 50 211 180 70 541 742 85 Cuts at: 4855 5926 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 541 399 329 268 211 180 132 132 108 101 94 85 70 50 22 BspEI T'CCGG_A Cuts at: 6372 6372 Size: 6709 BspHI T'CATG_A Cuts at: 2715 3318 4598 6328 2715 Size: 603 1280 1730 3096 Fragments arranged by size: 3096 1730 1280 603 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2087 3483 3727 3880 4064 844 Size: 1243 1396 244 153 184 3489 Fragments arranged by size: 3489 1396 1243 244 184 153 BsrBI CCG'CTC Cuts at: 1394 1823 2085 3001 3169 3454 3616 3958 4596 Size: 429 262 916 168 285 162 342 638 Cuts at: 4596 1394 Size: 3507 Fragments arranged by size: 3507 916 638 429 342 285 262 168 162 BsrDI GCAATG_nn' Cuts at: 65 1248 2491 3573 5204 5378 65 Size: 1183 1243 1082 1631 174 1396 Fragments arranged by size: 1631 1396 1243 1183 1082 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3516 3809 5353 5540 1700 Size: 110 1706 293 1544 187 2869 Fragments arranged by size: 2869 1706 1544 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6709 BssHII G'CGCG_C Cuts at: 1925 2788 3101 1925 Size: 863 313 5533 Fragments arranged by size: 5533 863 313 BssSI C'ACGA_G Cuts at: 2990 4769 5781 2990 Size: 1779 1012 3918 Fragments arranged by size: 3918 1779 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2605 2854 794 Size: 1811 249 4649 Fragments arranged by size: 4649 1811 249 BstBI TT'CG_AA Cuts at: 3552 5513 3552 Size: 1961 4748 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6709 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2257 3076 2011 Size: 246 819 5644 Fragments arranged by size: 5644 819 246 BstYI r'GATC_y Cuts at: 1972 2451 3141 3441 3715 4039 4406 4791 4808 Size: 479 690 300 274 324 367 385 17 Cuts at: 4808 6260 6346 6358 6704 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 690 479 385 367 346 324 300 274 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2164 2274 2642 3069 3075 3160 Size: 552 939 160 110 368 427 6 85 Cuts at: 3160 3644 3705 513 Size: 484 61 3517 Fragments arranged by size: 3517 939 552 484 427 368 160 110 85 61 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5970 Fragments arranged by size: 5970 683 56 BtsI GCAGTG_nn' Cuts at: 4225 5019 5039 4225 Size: 794 20 5895 Fragments arranged by size: 5895 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6709 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6674 DraI TTT'AAA Cuts at: 4013 4013 Size: 6709 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2924 5716 809 Size: 507 1608 2792 1802 Fragments arranged by size: 2792 1802 1608 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2161 2456 3072 Size: 54 1425 50 275 18 331 295 616 Cuts at: 3072 3157 3432 3807 4156 5045 6674 8 Size: 85 275 375 349 889 1629 43 Fragments arranged by size: 1629 1425 889 616 375 349 331 295 275 275 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 3807 4156 1537 Size: 275 1345 650 349 4090 Fragments arranged by size: 4090 1345 650 349 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2537 2807 3705 4639 1353 Size: 1184 270 898 934 3423 Fragments arranged by size: 3423 1184 934 898 270 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 3399 5296 5670 5816 387 Size: 1064 138 456 1354 1897 374 146 1280 Fragments arranged by size: 1897 1354 1280 1064 456 374 146 138 EcoICRI GAG'CTC Cuts at: 2289 2443 2289 Size: 154 6555 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2261 2872 3251 3374 3524 4772 Size: 393 401 228 611 379 123 150 1248 Cuts at: 4772 5369 6156 6424 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1248 787 611 597 401 393 379 268 228 150 123 EcoO109I rG'GnC_Cy Cuts at: 2156 2833 3247 3413 3483 3976 2156 Size: 677 414 166 70 493 4889 Fragments arranged by size: 4889 677 493 414 166 70 EcoRI G'AATT_C Cuts at: 4018 4396 4018 Size: 378 6331 FseI GG_CCGG'CC Cuts at: 3520 3520 Size: 6709 FspI TGC'GCA Cuts at: 5215 5215 Size: 6709 HaeII r_GCGC'y Cuts at: 1678 2040 2520 2895 3018 3062 3099 3307 3972 Size: 362 480 375 123 44 37 208 665 Cuts at: 3972 5856 1678 Size: 1884 2531 Fragments arranged by size: 2531 1884 665 480 375 362 208 123 44 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 3257 3289 5350 Size: 32 135 32 548 32 1105 32 2061 Cuts at: 5350 5382 5424 5456 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2061 1105 548 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4053 4299 669 Size: 988 281 2115 246 3079 Fragments arranged by size: 3079 2115 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6709 HpaI GTT'AAC Cuts at: 4299 4299 Size: 6709 KasI G'GCGC_C Cuts at: 2516 3014 3095 2516 Size: 498 81 6130 Fragments arranged by size: 6130 498 81 KpnI G_GTAC'C Cuts at: 2021 4034 2021 Size: 2013 4696 MfeI C'AATT_G Cuts at: 4308 4308 Size: 6709 MluI A'CGCG_T Cuts at: 6585 6585 Size: 6709 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2532 3615 4014 5823 6007 1305 Size: 1227 1083 399 1809 184 2007 Fragments arranged by size: 2007 1809 1227 1083 399 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2163 10 Size: 54 1425 343 331 4556 Fragments arranged by size: 4556 1425 343 331 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 3074 4667 5026 5185 518 Size: 648 1021 887 1593 359 159 2042 Fragments arranged by size: 2042 1593 1021 887 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2257 2494 2644 2914 3149 3162 3409 3422 Size: 651 237 150 270 235 13 247 13 Cuts at: 3422 3646 3707 4125 4802 5950 6195 1606 Size: 224 61 418 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 651 418 270 247 245 237 235 224 150 61 13 13 NaeI GCC'GGC Cuts at: 3518 3518 Size: 6709 NarI GG'CG_CC Cuts at: 2517 3015 3096 2517 Size: 498 81 6130 Fragments arranged by size: 6130 498 81 NcoI C'CATG_G Cuts at: 513 1065 2004 2164 3075 513 Size: 552 939 160 911 4147 Fragments arranged by size: 4147 939 911 552 160 NdeI CA'TA_TG Cuts at: 387 387 Size: 6709 NgoMIV G'CCGG_C Cuts at: 3516 3516 Size: 6709 NheI G'CTAG_C Cuts at: 1052 3394 1052 Size: 2342 4367 NotI GC'GGCC_GC Cuts at: 4156 4156 Size: 6709 NspI r_CATG'y Cuts at: 1132 1229 2857 4067 5612 1132 Size: 97 1628 1210 1545 2229 Fragments arranged by size: 2229 1628 1545 1210 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6709 PciI A'CATG_T Cuts at: 5608 5608 Size: 6709 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6709 PfoI T'CCnGG_A Cuts at: 2388 2436 2928 2388 Size: 48 492 6169 Fragments arranged by size: 6169 492 48 PmeI GTTT'AAAC Cuts at: 4013 4013 Size: 6709 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2576 2608 4778 4810 6291 6323 2576 Size: 32 2170 32 1481 32 2962 Fragments arranged by size: 2962 2170 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2833 2833 Size: 6709 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6709 PsiI TTA'TAA Cuts at: 4279 4279 Size: 6709 PspOMI G'GGCC_C Cuts at: 2155 3413 3483 2155 Size: 1258 70 5381 Fragments arranged by size: 5381 1258 70 PstI C_TGCA'G Cuts at: 830 2601 2913 3207 3723 4061 830 Size: 1771 312 294 516 338 3478 Fragments arranged by size: 3478 1771 516 338 312 294 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6709 PvuII CAG'CTG Cuts at: 2257 2257 Size: 6709 SacI G_AGCT'C Cuts at: 2291 2445 2291 Size: 154 6555 SacII CC_GC'GG Cuts at: 2645 3163 3647 3708 2645 Size: 518 484 61 5646 Fragments arranged by size: 5646 518 484 61 SalI G'TCGA_C Cuts at: 1936 4051 1936 Size: 2115 4594 SapI GCTCTTCn'nnn_ Cuts at: 2807 2807 Size: 6709 SbfI CC_TGCA'GG Cuts at: 4061 4061 Size: 6709 ScaI AGT'ACT Cuts at: 4957 6669 4957 Size: 1712 4997 SfcI C'TryA_G Cuts at: 826 1045 2597 2909 2953 3203 3211 3564 3719 Size: 219 1552 312 44 250 8 353 155 Cuts at: 3719 3964 4057 5192 5873 6064 826 Size: 245 93 1135 681 191 1471 Fragments arranged by size: 1552 1471 1135 681 353 312 250 245 219 191 155 93 44 8 SfiI GGCCn_nnn'nGGCC Cuts at: 3983 3983 Size: 6709 SfoI GGC'GCC Cuts at: 2518 3016 3097 2518 Size: 498 81 6130 Fragments arranged by size: 6130 498 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6709 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6709 SmaI CCC'GGG Cuts at: 1872 2514 4036 1872 Size: 642 1522 4545 Fragments arranged by size: 4545 1522 642 SmlI C'TyrA_G Cuts at: 820 1017 1943 2753 2765 4813 5714 5976 820 Size: 197 926 810 12 2048 901 262 1553 Fragments arranged by size: 2048 1553 926 901 810 262 197 12 SnaBI TAC'GTA Cuts at: 493 4500 493 Size: 4007 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6709 SphI G_CATG'C Cuts at: 1132 4067 1132 Size: 2935 3774 SspI AAT'ATT Cuts at: 5 52 4633 5 Size: 47 4581 2081 Fragments arranged by size: 4581 2081 47 StuI AGG'CCT Cuts at: 1517 3851 3884 1517 Size: 2334 33 4342 Fragments arranged by size: 4342 2334 33 StyI C'CwwG_G Cuts at: 513 1065 2004 2164 3075 4148 513 Size: 552 939 160 911 1073 3074 Fragments arranged by size: 3074 1073 939 911 552 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3934 4736 4753 4906 5091 5536 325 Size: 1255 2354 802 17 153 185 445 1498 Fragments arranged by size: 2354 1498 1255 802 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 2673 3948 Size: 274 80 33 51 1443 495 201 1275 Cuts at: 3948 4955 6667 96 Size: 1007 1712 138 Fragments arranged by size: 1712 1443 1275 1007 495 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2054 2086 2273 2305 3734 3766 6669 6701 2054 Size: 32 187 32 1429 32 2903 32 2062 Fragments arranged by size: 2903 2062 1429 187 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3586 1316 Size: 2270 4439 XbaI T'CTAG_A Cuts at: 4045 4045 Size: 6709 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6709 XmaI C'CCGG_G Cuts at: 1870 2512 4034 1870 Size: 642 1522 4545 Fragments arranged by size: 4545 1522 642 XmnI GAAnn'nnTTC Cuts at: 2816 4838 2816 Size: 2022 4687 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3633 4517 276 Size: 53 83 186 936 2099 884 2468 Fragments arranged by size: 2468 2099 936 884 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SrfI SwaI XcmI