(Circular) (Six-base) MAPSORT of: pFN21AA0584.seq Check: 4523 from: 1 to: 6583 pFN21AA0584 6583 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4393 278 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3926 1937 Size: 1989 4594 Acc65I G'GTAC_C Cuts at: 3705 3904 3705 Size: 199 6384 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2912 3182 4646 6030 6298 1632 Size: 1280 270 1464 1384 268 1917 Fragments arranged by size: 1917 1464 1384 1280 270 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 5482 6459 1203 Size: 4279 977 1327 Fragments arranged by size: 4279 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6583 AlwNI CAG_nnn'CTG Cuts at: 2276 2908 3532 4326 5898 2276 Size: 632 624 794 1572 2961 Fragments arranged by size: 2961 1572 794 632 624 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 3892 4118 4270 6379 1493 Size: 2399 226 152 2109 1697 Fragments arranged by size: 2399 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 2589 3908 1870 Size: 73 646 1319 4545 Fragments arranged by size: 4545 1319 646 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3697 3730 3697 Size: 33 6550 BamHI G'GATC_C Cuts at: 3913 4280 3913 Size: 367 6216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3705 3904 5359 618 Size: 325 217 627 1918 199 1455 1842 Fragments arranged by size: 1918 1842 1455 627 325 217 199 BanII G_rGCy'C Cuts at: 1391 2162 3902 1391 Size: 771 1740 4072 Fragments arranged by size: 4072 1740 771 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2922 3302 3318 928 Size: 971 1023 380 16 4193 Fragments arranged by size: 4193 1023 971 380 16 BbvCI CC'TCA_GC Cuts at: 2056 2056 Size: 6583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4774 4808 1903 Size: 34 2837 34 3678 Fragments arranged by size: 3678 2837 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4475 5685 4475 Size: 1210 5373 BclI T'GATC_A Cuts at: 1548 2278 1548 Size: 730 5853 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2267 5194 136 Size: 107 122 71 631 605 595 2927 1525 Fragments arranged by size: 2927 1525 631 605 595 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 3381 4000 3381 Size: 619 5964 Bme1580I G_kGCm'C Cuts at: 1790 3453 4644 5800 1790 Size: 1663 1191 1156 2573 Fragments arranged by size: 2573 1663 1191 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6583 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2977 3400 3772 Size: 416 53 83 399 24 1560 423 372 Cuts at: 3772 4314 5272 442 Size: 542 958 1753 Fragments arranged by size: 1753 1560 958 542 423 416 399 372 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2122 2122 Size: 6583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2603 2806 3196 5243 1239 Size: 1010 354 203 390 2047 2579 Fragments arranged by size: 2579 2047 1010 390 354 203 Bpu10I CC'TnA_GC Cuts at: 2056 2056 Size: 6583 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4708 5573 5871 4708 Size: 865 298 5420 Fragments arranged by size: 5420 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3716 5246 882 Size: 617 2217 1530 2219 Fragments arranged by size: 2219 2217 1530 617 BsaAI yAC'GTr Cuts at: 493 1204 4374 493 Size: 711 3170 2702 Fragments arranged by size: 3170 2702 711 BsaBI GATnn'nnATC Cuts at: 2261 6577 2261 Size: 4316 2267 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4390 4772 5407 275 Size: 53 83 186 936 2857 382 635 1451 Fragments arranged by size: 2857 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3075 5016 5414 5688 5835 6246 3075 Size: 1941 398 274 147 411 3412 Fragments arranged by size: 3412 1941 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6358 Fragments arranged by size: 6358 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2044 2138 1186 Size: 853 5 94 5631 Fragments arranged by size: 5631 853 94 5 BseYI C'CCAG_C Cuts at: 5786 6282 5786 Size: 496 6087 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2960 1163 Size: 573 1224 4786 Fragments arranged by size: 4786 1224 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4033 4794 5822 1540 Size: 275 186 2032 761 1028 2301 Fragments arranged by size: 2301 2032 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2868 3526 4644 4729 5800 2172 Size: 696 658 1118 85 1071 2955 Fragments arranged by size: 2955 1118 1071 696 658 85 BsmI GAATG_Cn' Cuts at: 4094 4187 4094 Size: 93 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2172 2868 3453 3526 3902 4644 Size: 399 372 10 696 585 73 376 742 Cuts at: 4644 4729 5800 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 696 585 399 376 372 85 73 10 BspEI T'CCGG_A Cuts at: 6246 6246 Size: 6583 BspHI T'CATG_A Cuts at: 3039 4472 6202 3039 Size: 1433 1730 3420 Fragments arranged by size: 3420 1730 1433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2886 3938 844 Size: 2042 1052 3489 Fragments arranged by size: 3489 2042 1052 BsrBI CCG'CTC Cuts at: 1394 1823 2101 2233 4470 1394 Size: 429 278 132 2237 3507 Fragments arranged by size: 3507 2237 429 278 132 BsrDI GCAATG_nn' Cuts at: 65 1248 2958 3820 5078 5252 65 Size: 1183 1710 862 1258 174 1396 Fragments arranged by size: 1710 1396 1258 1183 862 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2128 5227 5414 1700 Size: 110 318 3099 187 2869 Fragments arranged by size: 3099 2869 318 187 110 BsrGI T'GTAC_A Cuts at: 96 2329 3591 96 Size: 2233 1262 3088 Fragments arranged by size: 3088 2233 1262 BssHII G'CGCG_C Cuts at: 1925 2012 2084 1925 Size: 87 72 6424 Fragments arranged by size: 6424 87 72 BssSI C'ACGA_G Cuts at: 2171 4643 5655 2171 Size: 2472 1012 3099 Fragments arranged by size: 3099 2472 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 794 Size: 1412 5171 BstBI TT'CG_AA Cuts at: 2461 5387 2461 Size: 2926 3657 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6583 BstXI CCAn_nnnn'nTGG Cuts at: 2409 2409 Size: 6583 BstYI r'GATC_y Cuts at: 1972 3199 3913 4280 4665 4682 6134 6220 6232 Size: 1227 714 367 385 17 1452 86 12 Cuts at: 6232 6578 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1227 714 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2628 2732 2628 Size: 104 6479 BtgI C'CryG_G Cuts at: 513 1065 2004 2163 2703 513 Size: 552 939 159 540 4393 Fragments arranged by size: 4393 939 552 540 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5844 Fragments arranged by size: 5844 683 56 BtsI GCAGTG_nn' Cuts at: 2906 3453 4099 4893 4913 2906 Size: 547 646 794 20 4576 Fragments arranged by size: 4576 794 646 547 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3887 3887 Size: 6583 DraIII CAC_nnn'GTG Cuts at: 2287 2350 2287 Size: 63 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2807 5590 809 Size: 507 1491 2783 1802 Fragments arranged by size: 2783 1802 1491 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2411 3753 Size: 54 1425 50 275 18 429 152 1342 Cuts at: 3753 4030 4919 6548 8 Size: 277 889 1629 43 Fragments arranged by size: 1629 1425 1342 889 429 277 275 152 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4030 1537 Size: 275 2218 4090 Fragments arranged by size: 4090 2218 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2672 3410 4513 1353 Size: 1319 738 1103 3423 Fragments arranged by size: 3423 1319 1103 738 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5170 5544 5690 387 Size: 1064 138 3581 374 146 1280 Fragments arranged by size: 3581 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2603 2806 2912 3182 3196 4646 Size: 393 617 354 203 106 270 14 1450 Cuts at: 4646 5243 6030 6298 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1450 787 617 597 393 354 270 268 203 106 14 EcoNI CCTnn'n_nnAGG Cuts at: 2735 2735 Size: 6583 EcoO109I rG'GnC_Cy Cuts at: 2593 2646 3640 3832 2593 Size: 53 994 192 5344 Fragments arranged by size: 5344 994 192 53 EcoRI G'AATT_C Cuts at: 3892 4270 3892 Size: 378 6205 FspI TGC'GCA Cuts at: 3086 5089 3086 Size: 2003 4580 HaeII r_GCGC'y Cuts at: 1678 5730 1678 Size: 4052 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2326 2358 2524 2556 2947 Size: 32 135 32 754 32 166 32 391 Cuts at: 2947 2979 5224 5256 5298 5330 1373 Size: 32 2245 32 42 32 2626 Fragments arranged by size: 2626 2245 754 391 166 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2509 3927 4173 669 Size: 988 281 571 1418 246 3079 Fragments arranged by size: 3079 1418 988 571 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KpnI G_GTAC'C Cuts at: 3709 3908 3709 Size: 199 6384 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3517 5697 5881 1305 Size: 2212 2180 184 2007 Fragments arranged by size: 2212 2180 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2413 3755 10 Size: 54 1425 343 429 152 1342 2838 Fragments arranged by size: 2838 1425 1342 429 343 152 54 MslI CAynn'nnrTG Cuts at: 518 1166 2843 2936 4541 4900 5059 518 Size: 648 1677 93 1605 359 159 2042 Fragments arranged by size: 2042 1677 1605 648 359 159 93 MspA1I CmG'CkG Cuts at: 1606 3160 3385 3999 4676 5824 6069 1606 Size: 1554 225 614 677 1148 245 2120 Fragments arranged by size: 2120 1554 1148 677 614 245 225 NcoI C'CATG_G Cuts at: 513 1065 2004 2703 513 Size: 552 939 699 4393 Fragments arranged by size: 4393 939 699 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6583 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 3779 3941 5486 1132 Size: 97 2550 162 1545 2229 Fragments arranged by size: 2550 2229 1545 162 97 PasI CC'CwG_GG Cuts at: 1348 3496 1348 Size: 2148 4435 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 3735 3762 1108 Size: 2627 27 3929 Fragments arranged by size: 3929 2627 27 PfoI T'CCnGG_A Cuts at: 2139 3788 2139 Size: 1649 4934 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3654 3686 4652 4684 6165 6197 3654 Size: 32 966 32 1481 32 4040 Fragments arranged by size: 4040 1481 966 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2646 3640 2646 Size: 994 5589 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PstI C_TGCA'G Cuts at: 830 2157 2913 3661 3935 830 Size: 1327 756 748 274 3478 Fragments arranged by size: 3478 1327 756 748 274 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 3160 3385 3160 Size: 225 6358 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 4831 6543 4831 Size: 1712 4871 SexAI A'CCwGG_T Cuts at: 3465 3465 Size: 6583 SfcI C'TryA_G Cuts at: 826 1045 2153 2909 3657 3808 3931 5066 5747 Size: 219 1108 756 748 151 123 1135 681 Cuts at: 5747 5938 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1108 756 748 681 219 191 151 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 3910 1872 Size: 2038 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4687 5588 5850 820 Size: 197 926 2744 901 262 1553 Fragments arranged by size: 2744 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 3941 1132 Size: 2809 3774 SspI AAT'ATT Cuts at: 5 52 2307 3175 4507 5 Size: 47 2255 868 1332 2081 Fragments arranged by size: 2255 2081 1332 868 47 StuI AGG'CCT Cuts at: 1517 2362 2744 3217 1517 Size: 845 382 473 4883 Fragments arranged by size: 4883 845 473 382 StyI C'CwwG_G Cuts at: 513 1065 2004 2703 4022 513 Size: 552 939 699 1319 3074 Fragments arranged by size: 3074 1319 939 699 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4610 4627 4780 4965 5410 325 Size: 1255 3030 17 153 185 445 1498 Fragments arranged by size: 3030 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2329 3591 3615 Size: 274 80 33 51 1443 352 1262 24 Cuts at: 3615 4829 6541 96 Size: 1214 1712 138 Fragments arranged by size: 1712 1443 1262 1214 352 274 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6543 6575 6543 Size: 32 6551 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6583 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2170 2254 2170 Size: 84 6499 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6583 XmaI C'CCGG_G Cuts at: 1870 3908 1870 Size: 2038 4545 XmnI GAAnn'nnTTC Cuts at: 3650 4712 3650 Size: 1062 5521 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4391 276 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI