(Circular) (Six-base) MAPSORT of: pFN21AA0571.seq Check: 3153 from: 1 to: 6619 pFN21AA0571 6619 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3284 3284 Size: 6619 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2823 3315 3962 1937 Size: 886 492 647 4594 Fragments arranged by size: 4594 886 647 492 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3255 4682 6066 6334 1632 Size: 1623 1427 1384 268 1917 Fragments arranged by size: 1917 1623 1427 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 5518 6495 1203 Size: 4315 977 1327 Fragments arranged by size: 4315 1327 977 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 2600 5348 2600 Size: 2748 3871 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6619 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2115 1184 Size: 931 5688 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2033 2065 2033 Size: 32 6587 AlwNI CAG_nnn'CTG Cuts at: 3665 3850 4362 5934 3665 Size: 185 512 1572 4350 Fragments arranged by size: 4350 1572 512 185 ApaI G_GGCC'C Cuts at: 3340 3340 Size: 6619 ApaLI G'TGCA_C Cuts at: 3383 4676 5832 3383 Size: 1293 1156 4170 Fragments arranged by size: 4170 1293 1156 ApoI r'AATT_y Cuts at: 1493 2669 3928 4154 4306 6415 1493 Size: 1176 1259 226 152 2109 1697 Fragments arranged by size: 2109 1697 1259 1176 226 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 3944 1870 Size: 73 2001 4545 Fragments arranged by size: 4545 2001 73 AvrII C'CTAG_G Cuts at: 2870 2870 Size: 6619 BamHI G'GATC_C Cuts at: 3949 4316 3949 Size: 367 6252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2531 3908 3940 5395 618 Size: 325 217 627 744 1377 32 1455 1842 Fragments arranged by size: 1842 1455 1377 744 627 325 217 32 BanII G_rGCy'C Cuts at: 1391 2325 3332 3340 3758 3938 1391 Size: 934 1007 8 418 180 4072 Fragments arranged by size: 4072 1007 934 418 180 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3243 3845 928 Size: 971 1344 602 3702 Fragments arranged by size: 3702 1344 971 602 BbvCI CC'TCA_GC Cuts at: 2253 2287 2365 2997 2253 Size: 34 78 632 5875 Fragments arranged by size: 5875 632 78 34 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2788 2822 3016 3050 4810 4844 1903 Size: 34 851 34 194 34 1760 34 3678 Fragments arranged by size: 3678 1760 851 194 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4511 5721 4511 Size: 1210 5409 BclI T'GATC_A Cuts at: 1548 1548 Size: 6619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2656 2739 5230 Size: 107 122 71 631 605 984 83 2491 Cuts at: 5230 136 Size: 1525 Fragments arranged by size: 2491 1525 984 631 605 122 107 83 71 BglII A'GATC_T Cuts at: 2232 3029 6614 2232 Size: 797 3585 2237 Fragments arranged by size: 3585 2237 797 BlpI GC'TnA_GC Cuts at: 2319 2324 4036 2319 Size: 5 1712 4902 Fragments arranged by size: 4902 1712 5 Bme1580I G_kGCm'C Cuts at: 1790 2534 3252 3340 3387 4680 5836 1790 Size: 744 718 88 47 1293 1156 2573 Fragments arranged by size: 2573 1293 1156 744 718 88 47 BmgBI CAC'GTC Cuts at: 1318 3880 1318 Size: 2562 4057 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3568 3653 3675 Size: 416 53 83 399 24 2151 85 22 Cuts at: 3675 4350 5308 442 Size: 675 958 1753 Fragments arranged by size: 2151 1753 958 675 416 399 85 83 53 24 22 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 2365 3547 5279 1239 Size: 1059 67 1182 1732 2579 Fragments arranged by size: 2579 1732 1182 1059 67 Bpu10I CC'TnA_GC Cuts at: 2253 2287 2365 2997 2253 Size: 34 78 632 5875 Fragments arranged by size: 5875 632 78 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4744 5609 5907 4744 Size: 865 298 5456 Fragments arranged by size: 5456 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2998 3104 5282 882 Size: 617 1499 106 2178 2219 Fragments arranged by size: 2219 2178 1499 617 106 BsaAI yAC'GTr Cuts at: 493 1204 4410 493 Size: 711 3206 2702 Fragments arranged by size: 3206 2702 711 BsaBI GATnn'nnATC Cuts at: 6613 6613 Size: 6619 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2780 4426 4808 5443 Size: 53 83 186 936 1247 1646 382 635 Cuts at: 5443 275 Size: 1451 Fragments arranged by size: 1646 1451 1247 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5052 5450 5724 5871 6282 5052 Size: 398 274 147 411 5389 Fragments arranged by size: 5389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2063 3551 3581 1178 Size: 30 165 30 630 30 1488 30 4216 Fragments arranged by size: 4216 1488 630 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2569 3559 1186 Size: 1084 299 990 4246 Fragments arranged by size: 4246 1084 990 299 BseYI C'CCAG_C Cuts at: 2783 3395 5822 6318 2783 Size: 612 2427 496 3084 Fragments arranged by size: 3084 2427 612 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2818 3165 1163 Size: 573 1082 347 4617 Fragments arranged by size: 4617 1082 573 347 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3218 4069 4830 5858 1540 Size: 275 186 1217 851 761 1028 2301 Fragments arranged by size: 2301 1217 1028 851 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2264 2325 3387 3716 3758 4680 4765 5836 2264 Size: 61 1062 329 42 922 85 1071 3047 Fragments arranged by size: 3047 1071 1062 922 329 85 61 42 BsmI GAATG_Cn' Cuts at: 4130 4223 4130 Size: 93 6526 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 2325 2534 3252 3332 3340 3387 Size: 399 474 61 209 718 80 8 47 Cuts at: 3387 3716 3758 3938 4680 4765 5836 1391 Size: 329 42 180 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 718 474 399 329 209 180 85 80 61 47 42 8 BspEI T'CCGG_A Cuts at: 6282 6282 Size: 6619 BspHI T'CATG_A Cuts at: 2550 4508 6238 2550 Size: 1958 1730 2931 Fragments arranged by size: 2931 1958 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2083 3284 3974 844 Size: 1239 1201 690 3489 Fragments arranged by size: 3489 1239 1201 690 BsrBI CCG'CTC Cuts at: 1394 1823 2010 2307 4506 1394 Size: 429 187 297 2199 3507 Fragments arranged by size: 3507 2199 429 297 187 BsrDI GCAATG_nn' Cuts at: 65 1248 2596 3078 5114 5288 65 Size: 1183 1348 482 2036 174 1396 Fragments arranged by size: 2036 1396 1348 1183 482 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2291 2655 3150 3716 5263 5450 1700 Size: 110 481 364 495 566 1547 187 2869 Fragments arranged by size: 2869 1547 566 495 481 364 187 110 BsrGI T'GTAC_A Cuts at: 96 2569 2766 96 Size: 2473 197 3949 Fragments arranged by size: 3949 2473 197 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 4679 5691 4679 Size: 1012 5607 BstAPI GCAn_nnn'nTGC Cuts at: 794 2504 2513 794 Size: 1710 9 4900 Fragments arranged by size: 4900 1710 9 BstBI TT'CG_AA Cuts at: 5423 5423 Size: 6619 BstEII G'GTnAC_C Cuts at: 1184 2012 1184 Size: 828 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2237 2591 2911 3277 2237 Size: 354 320 366 5579 Fragments arranged by size: 5579 366 354 320 BstYI r'GATC_y Cuts at: 1972 2232 2865 3029 3949 4316 4701 4718 6170 Size: 260 633 164 920 367 385 17 1452 Cuts at: 6170 6256 6268 6614 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 920 633 385 367 346 260 164 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2457 3479 2457 Size: 1022 5597 BtgI C'CryG_G Cuts at: 513 1065 2626 2713 3282 513 Size: 552 1561 87 569 3850 Fragments arranged by size: 3850 1561 569 552 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 4135 4929 4949 4135 Size: 794 20 5805 Fragments arranged by size: 5805 794 20 ClaI AT'CG_AT Cuts at: 1554 3498 1554 Size: 1944 4675 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DraIII CAC_nnn'GTG Cuts at: 2796 3131 2796 Size: 335 6284 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5626 809 Size: 507 4310 1802 Fragments arranged by size: 4310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2716 2841 2913 Size: 54 1425 50 275 18 886 125 72 Cuts at: 2913 3279 4066 4955 6584 8 Size: 366 787 889 1629 43 Fragments arranged by size: 1629 1425 889 886 787 366 275 125 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4066 1537 Size: 275 2254 4090 Fragments arranged by size: 4090 2254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 2314 2488 3627 3704 4549 1353 Size: 837 124 174 1139 77 845 3423 Fragments arranged by size: 3423 1139 845 837 174 124 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5206 5580 5726 387 Size: 1064 138 3617 374 146 1280 Fragments arranged by size: 3617 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2323 3756 2323 Size: 1433 5186 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2298 2365 3255 3547 4682 5279 6066 Size: 393 666 67 890 292 1135 597 787 Cuts at: 6066 6334 1239 Size: 268 1524 Fragments arranged by size: 1524 1135 890 787 666 597 393 292 268 67 EcoO109I rG'GnC_Cy Cuts at: 2812 3336 3392 3847 2812 Size: 524 56 455 5584 Fragments arranged by size: 5584 524 455 56 EcoRI G'AATT_C Cuts at: 2669 3928 4306 2669 Size: 1259 378 4982 Fragments arranged by size: 4982 1259 378 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 5766 1678 Size: 4088 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2586 2602 2618 Size: 32 135 32 773 32 209 16 16 Cuts at: 2618 2634 2866 2898 2997 3029 5260 5292 5334 Size: 16 232 32 99 32 2231 32 42 Cuts at: 5334 5366 1373 Size: 32 2626 Fragments arranged by size: 2626 2231 773 232 209 135 99 42 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 2605 3430 3963 4209 669 Size: 988 281 667 825 533 246 3079 Fragments arranged by size: 3079 988 825 667 533 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6619 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5733 5917 1305 Size: 4428 184 2007 Fragments arranged by size: 4428 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2718 2915 3281 10 Size: 54 1425 343 886 197 366 3348 Fragments arranged by size: 3348 1425 886 366 343 197 54 MslI CAynn'nnrTG Cuts at: 518 1166 2909 2983 3323 4577 4936 5095 518 Size: 648 1743 74 340 1254 359 159 2042 Fragments arranged by size: 2042 1743 1254 648 359 340 159 74 MspA1I CmG'CkG Cuts at: 1606 2318 2567 4035 4712 5860 6105 1606 Size: 712 249 1468 677 1148 245 2120 Fragments arranged by size: 2120 1468 1148 712 677 249 245 NaeI GCC'GGC Cuts at: 2657 2657 Size: 6619 NcoI C'CATG_G Cuts at: 513 1065 2626 2713 3282 513 Size: 552 1561 87 569 3850 Fragments arranged by size: 3850 1561 569 552 87 NdeI CA'TA_TG Cuts at: 387 387 Size: 6619 NgoMIV G'CCGG_C Cuts at: 2655 2655 Size: 6619 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 4066 4066 Size: 6619 NspI r_CATG'y Cuts at: 1132 1229 2431 3977 5522 1132 Size: 97 1202 1546 1545 2229 Fragments arranged by size: 2229 1546 1545 1202 97 PasI CC'CwG_GG Cuts at: 1348 2584 3332 1348 Size: 1236 748 4635 Fragments arranged by size: 4635 1236 748 PciI A'CATG_T Cuts at: 5518 5518 Size: 6619 PflMI CCAn_nnn'nTGG Cuts at: 1108 3131 3534 1108 Size: 2023 403 4193 Fragments arranged by size: 4193 2023 403 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2033 2065 4688 4720 6201 6233 2033 Size: 32 2623 32 1481 32 2419 Fragments arranged by size: 2623 2419 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2812 2812 Size: 6619 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6619 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PspOMI G'GGCC_C Cuts at: 3336 3336 Size: 6619 PstI C_TGCA'G Cuts at: 830 2133 3855 3971 830 Size: 1303 1722 116 3478 Fragments arranged by size: 3478 1722 1303 116 PvuI CG_AT'CG Cuts at: 2001 3218 2001 Size: 1217 5402 PvuII CAG'CTG Cuts at: 2318 2567 2318 Size: 249 6370 SacI G_AGCT'C Cuts at: 2325 3758 2325 Size: 1433 5186 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SapI GCTCTTCn'nnn_ Cuts at: 2314 2488 3704 2314 Size: 174 1216 5229 Fragments arranged by size: 5229 1216 174 SbfI CC_TGCA'GG Cuts at: 3971 3971 Size: 6619 ScaI AGT'ACT Cuts at: 3578 4867 6579 3578 Size: 1289 1712 3618 Fragments arranged by size: 3618 1712 1289 SexAI A'CCwGG_T Cuts at: 2178 2178 Size: 6619 SfcI C'TryA_G Cuts at: 826 1045 2129 2635 3851 3967 5102 5783 5974 Size: 219 1084 506 1216 116 1135 681 191 Cuts at: 5974 826 Size: 1471 Fragments arranged by size: 1471 1216 1135 1084 681 506 219 191 116 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 3946 1872 Size: 2074 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4723 5624 5886 820 Size: 197 926 2780 901 262 1553 Fragments arranged by size: 2780 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3977 1132 Size: 2845 3774 SspI AAT'ATT Cuts at: 5 52 4543 5 Size: 47 4491 2081 Fragments arranged by size: 4491 2081 47 StuI AGG'CCT Cuts at: 1517 2106 1517 Size: 589 6030 StyI C'CwwG_G Cuts at: 513 1065 2626 2713 2724 2870 3282 3903 4058 Size: 552 1561 87 11 146 412 621 155 Cuts at: 4058 513 Size: 3074 Fragments arranged by size: 3074 1561 621 552 412 155 146 87 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3611 4646 4663 4816 5001 5446 325 Size: 1255 2031 1035 17 153 185 445 1498 Fragments arranged by size: 2031 1498 1255 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2569 2766 3576 Size: 274 80 33 51 1443 592 197 810 Cuts at: 3576 4865 6577 96 Size: 1289 1712 138 Fragments arranged by size: 1712 1443 1289 810 592 274 197 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3679 3711 6579 6611 3679 Size: 32 2868 32 3687 Fragments arranged by size: 3687 2868 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6619 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 3944 1870 Size: 2074 4545 XmnI GAAnn'nnTTC Cuts at: 2281 4748 2281 Size: 2467 4152 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI