(Circular) (Six-base) MAPSORT of: pFN21AA0546.seq Check: 7886 from: 1 to: 10435 pFN21AA0546 10435 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3169 3169 Size: 10435 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8245 278 Size: 53 83 186 936 6709 2468 Fragments arranged by size: 6709 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5930 7778 1937 Size: 3993 1848 4594 Fragments arranged by size: 4594 3993 1848 Acc65I G'GTAC_C Cuts at: 7756 7756 Size: 10435 AclI AA'CG_TT Cuts at: 1563 8562 8935 1563 Size: 6999 373 3063 Fragments arranged by size: 6999 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3125 3405 3495 3843 4338 4421 4617 4664 Size: 1493 280 90 348 495 83 196 47 Cuts at: 4664 5103 5706 5893 8498 9882 10150 1632 Size: 439 603 187 2605 1384 268 1917 Fragments arranged by size: 2605 1917 1493 1384 603 495 439 348 280 268 196 187 90 83 47 AflII C'TTAA_G Cuts at: 820 1017 5177 820 Size: 197 4160 6078 Fragments arranged by size: 6078 4160 197 AflIII A'CryG_T Cuts at: 1203 2277 5582 6726 9334 10311 1203 Size: 1074 3305 1144 2608 977 1327 Fragments arranged by size: 3305 2608 1327 1144 1074 977 AgeI A'CCGG_T Cuts at: 9266 9266 Size: 10435 AhdI GACnn_n'nnGTC Cuts at: 5659 9164 5659 Size: 3505 6930 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10435 AlwNI CAG_nnn'CTG Cuts at: 2251 3518 6919 7466 7672 8178 9750 2251 Size: 1267 3401 547 206 506 1572 2936 Fragments arranged by size: 3401 2936 1572 1267 547 506 206 ApaLI G'TGCA_C Cuts at: 6492 8492 9648 6492 Size: 2000 1156 7279 Fragments arranged by size: 7279 2000 1156 ApoI r'AATT_y Cuts at: 1493 2257 3040 3426 4549 4644 4689 4941 5021 Size: 764 783 386 1123 95 45 252 80 Cuts at: 5021 5106 6210 6605 6626 7037 7088 7204 7418 Size: 85 1104 395 21 411 51 116 214 Cuts at: 7418 7424 7517 7744 7970 8122 10231 1493 Size: 6 93 227 226 152 2109 1697 Fragments arranged by size: 2109 1697 1123 1104 783 764 411 395 386 252 227 226 214 152 116 95 93 85 80 51 45 21 6 AseI AT'TA_AT Cuts at: 160 1032 8989 160 Size: 872 7957 1606 Fragments arranged by size: 7957 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10435 AvaI C'yCGr_G Cuts at: 1870 1943 2187 4758 7760 1870 Size: 73 244 2571 3002 4545 Fragments arranged by size: 4545 3002 2571 244 73 AvrII C'CTAG_G Cuts at: 4046 4046 Size: 10435 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5982 6015 5982 Size: 33 10402 BamHI G'GATC_C Cuts at: 2546 7397 7765 8132 2546 Size: 4851 368 367 4849 Fragments arranged by size: 4851 4849 368 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6216 7756 9211 618 Size: 325 217 627 4429 1540 1455 1842 Fragments arranged by size: 4429 1842 1540 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2372 2583 2603 5519 7754 1391 Size: 981 211 20 2916 2235 4072 Fragments arranged by size: 4072 2916 2235 981 211 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4076 4409 928 Size: 971 2177 333 6954 Fragments arranged by size: 6954 2177 971 333 BbvCI CC'TCA_GC Cuts at: 2252 2252 Size: 10435 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8626 8660 1903 Size: 34 6689 34 3678 Fragments arranged by size: 6689 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3598 4044 5983 8327 9537 3598 Size: 446 1939 2344 1210 4496 Fragments arranged by size: 4496 2344 1939 1210 446 BclI T'GATC_A Cuts at: 1548 1548 Size: 10435 BfrBI ATG'CAT Cuts at: 2727 5208 6515 2727 Size: 2481 1307 6647 Fragments arranged by size: 6647 2481 1307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9046 136 Size: 107 122 71 631 605 7374 1525 Fragments arranged by size: 7374 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3433 5138 10430 3433 Size: 1705 5292 3438 Fragments arranged by size: 5292 3438 1705 BlpI GC'TnA_GC Cuts at: 4239 7852 4239 Size: 3613 6822 Bme1580I G_kGCm'C Cuts at: 1790 4969 6496 8496 9652 1790 Size: 3179 1527 2000 1156 2573 Fragments arranged by size: 3179 2573 2000 1527 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10435 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 8166 9124 442 Size: 416 53 83 399 24 6749 958 1753 Fragments arranged by size: 6749 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2494 3612 3672 3957 4836 4983 5988 Size: 781 474 1118 60 285 879 147 1005 Cuts at: 5988 6282 6869 9095 1239 Size: 294 587 2226 2579 Fragments arranged by size: 2579 2226 1118 1005 879 781 587 474 294 285 147 60 Bpu10I CC'TnA_GC Cuts at: 2252 5513 2252 Size: 3261 7174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6083 8560 9425 9723 6083 Size: 2477 865 298 6795 Fragments arranged by size: 6795 2477 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2343 2625 9098 882 Size: 617 844 282 6473 2219 Fragments arranged by size: 6473 2219 844 617 282 BsaAI yAC'GTr Cuts at: 493 1204 6361 8226 493 Size: 711 5157 1865 2702 Fragments arranged by size: 5157 2702 1865 711 BsaBI GATnn'nnATC Cuts at: 10429 10429 Size: 10435 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 8242 8624 9259 275 Size: 53 83 186 936 6709 382 635 1451 Fragments arranged by size: 6709 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2334 4303 5545 8868 9266 9540 9687 10098 2334 Size: 1969 1242 3323 398 274 147 411 2671 Fragments arranged by size: 3323 2671 1969 1242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3610 3640 3798 3828 1178 Size: 30 165 30 2207 30 158 30 7785 Fragments arranged by size: 7785 2207 165 158 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2166 2502 2517 2550 2553 2882 3505 3576 Size: 980 336 15 33 3 329 623 71 Cuts at: 3576 3783 3884 5823 1186 Size: 207 101 1939 5798 Fragments arranged by size: 5798 1939 980 623 336 329 207 101 71 33 15 3 BseYI C'CCAG_C Cuts at: 2306 4420 7566 9638 10134 2306 Size: 2114 3146 2072 496 2607 Fragments arranged by size: 3146 2607 2114 2072 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2539 2795 5709 6284 6449 6821 1163 Size: 573 803 256 2914 575 165 372 4777 Fragments arranged by size: 4777 2914 803 575 573 372 256 165 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2396 7885 8646 9674 1540 Size: 275 186 12 383 5489 761 1028 2301 Fragments arranged by size: 5489 2301 1028 761 383 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2372 2603 5519 6143 6496 8496 8581 9652 2372 Size: 231 2916 624 353 2000 85 1071 3155 Fragments arranged by size: 3155 2916 2000 1071 624 353 231 85 BsmI GAATG_Cn' Cuts at: 2729 4873 5210 6517 7015 7570 7946 8039 2729 Size: 2144 337 1307 498 555 376 93 5125 Fragments arranged by size: 5125 2144 1307 555 498 376 337 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2372 2583 2603 4969 5519 6143 6496 Size: 399 582 211 20 2366 550 624 353 Cuts at: 6496 7754 8496 8581 9652 1391 Size: 1258 742 85 1071 2174 Fragments arranged by size: 2366 2174 1258 1071 742 624 582 550 399 353 211 85 20 BspEI T'CCGG_A Cuts at: 10098 10098 Size: 10435 BspHI T'CATG_A Cuts at: 3468 5047 8324 10054 3468 Size: 1579 3277 1730 3849 Fragments arranged by size: 3849 3277 1730 1579 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3169 4468 7790 844 Size: 2325 1299 3322 3489 Fragments arranged by size: 3489 3322 2325 1299 BsrBI CCG'CTC Cuts at: 1394 1823 2287 2410 8322 1394 Size: 429 464 123 5912 3507 Fragments arranged by size: 5912 3507 464 429 123 BsrDI GCAATG_nn' Cuts at: 65 1248 4470 6873 7666 8930 9104 65 Size: 1183 3222 2403 793 1264 174 1396 Fragments arranged by size: 3222 2403 1396 1264 1183 793 174 BsrFI r'CCGG_y Cuts at: 1700 1810 9079 9266 1700 Size: 110 7269 187 2869 Fragments arranged by size: 7269 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3223 6085 96 Size: 3127 2862 4446 Fragments arranged by size: 4446 3127 2862 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10435 BssSI C'ACGA_G Cuts at: 8495 9507 8495 Size: 1012 9423 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 10435 BstBI TT'CG_AA Cuts at: 9239 9239 Size: 10435 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10435 BstXI CCAn_nnnn'nTGG Cuts at: 5880 6213 7582 5880 Size: 333 1369 8733 Fragments arranged by size: 8733 1369 333 BstYI r'GATC_y Cuts at: 1972 2198 2546 2782 3433 3514 3826 5138 7138 Size: 226 348 236 651 81 312 1312 2000 Cuts at: 7138 7397 7765 8132 8517 8534 9986 10072 10084 Size: 259 368 367 385 17 1452 86 12 Cuts at: 10084 10430 1972 Size: 346 1977 Fragments arranged by size: 2000 1977 1452 1312 651 385 368 367 348 346 312 259 236 226 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 2297 2865 3524 3720 2297 Size: 568 659 196 9012 Fragments arranged by size: 9012 659 568 196 BtgI C'CryG_G Cuts at: 513 1065 2004 2162 6689 513 Size: 552 939 158 4527 4259 Fragments arranged by size: 4527 4259 939 552 158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6028 487 Size: 683 56 4802 4894 Fragments arranged by size: 4894 4802 683 56 BtsI GCAGTG_nn' Cuts at: 4776 6759 6981 7498 7951 8745 8765 4776 Size: 1983 222 517 453 794 20 6446 Fragments arranged by size: 6446 1983 794 517 453 222 20 ClaI AT'CG_AT Cuts at: 1554 3463 6568 1554 Size: 1909 3105 5421 Fragments arranged by size: 5421 3105 1909 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4752 4787 529 Size: 35 4188 35 6177 Fragments arranged by size: 6177 4188 35 35 DraI TTT'AAA Cuts at: 2694 4545 5322 6165 7108 7739 2694 Size: 1851 777 843 943 631 5390 Fragments arranged by size: 5390 1851 943 843 777 631 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9442 809 Size: 507 8126 1802 Fragments arranged by size: 8126 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2393 3261 4773 Size: 54 1425 50 275 18 563 868 1512 Cuts at: 4773 7719 7882 8771 10400 8 Size: 2946 163 889 1629 43 Fragments arranged by size: 2946 1629 1512 1425 889 868 563 275 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2393 7882 1537 Size: 275 581 5489 4090 Fragments arranged by size: 5489 4090 581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2224 2242 2591 2760 3096 3144 3357 3387 Size: 871 18 349 169 336 48 213 30 Cuts at: 3387 3861 4347 4743 5004 5457 7278 8365 1353 Size: 474 486 396 261 453 1821 1087 3423 Fragments arranged by size: 3423 1821 1087 871 486 474 453 396 349 336 261 213 169 48 30 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2220 2334 9022 9396 9542 387 Size: 1064 138 631 114 6688 374 146 1280 Fragments arranged by size: 6688 1280 1064 631 374 146 138 114 EcoICRI GAG'CTC Cuts at: 2370 2601 5517 2370 Size: 231 2916 7288 Fragments arranged by size: 7288 2916 231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2494 3125 3405 3495 3612 3672 Size: 393 388 474 631 280 90 117 60 Cuts at: 3672 3843 3957 4338 4421 4617 4664 4836 4983 Size: 171 114 381 83 196 47 172 147 Cuts at: 4983 5103 5706 5893 5988 6282 6869 8498 9095 Size: 120 603 187 95 294 587 1629 597 Cuts at: 9095 9882 10150 1239 Size: 787 268 1524 Fragments arranged by size: 1629 1524 787 631 603 597 587 474 393 388 381 294 280 268 196 187 172 171 147 120 117 114 95 90 83 60 47 EcoNI CCTnn'n_nnAGG Cuts at: 2532 2532 Size: 10435 EcoO109I rG'GnC_Cy Cuts at: 2303 4076 2303 Size: 1773 8662 EcoRI G'AATT_C Cuts at: 6605 7418 7744 8122 6605 Size: 813 326 378 8918 Fragments arranged by size: 8918 813 378 326 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2084 2705 3525 3917 5157 2084 Size: 621 820 392 1240 7362 Fragments arranged by size: 7362 1240 820 621 392 FspI TGC'GCA Cuts at: 8941 8941 Size: 10435 HaeII r_GCGC'y Cuts at: 1678 9582 1678 Size: 7904 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2101 2133 3059 3091 3095 Size: 32 135 32 529 32 926 32 4 Cuts at: 3095 3127 3929 3961 4042 4074 4142 4174 4478 Size: 32 802 32 81 32 68 32 304 Cuts at: 4478 4510 5645 5677 5756 5788 5912 5944 7169 Size: 32 1135 32 79 32 124 32 1225 Cuts at: 7169 7201 7351 7383 7490 7522 9076 9108 9150 Size: 32 150 32 107 32 1554 32 42 Cuts at: 9150 9182 1373 Size: 32 2626 Fragments arranged by size: 2626 1554 1225 1135 926 802 529 304 150 135 124 107 81 79 68 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 3207 4182 4657 6667 7779 8025 Size: 988 281 1269 975 475 2010 1112 246 Cuts at: 8025 669 Size: 3079 Fragments arranged by size: 3079 2010 1269 1112 988 975 475 281 246 HindIII A'AGCT_T Cuts at: 748 4245 4676 4725 6101 6310 6759 748 Size: 3497 431 49 1376 209 449 4424 Fragments arranged by size: 4424 3497 1376 449 431 209 49 HpaI GTT'AAC Cuts at: 4657 6667 8025 4657 Size: 2010 1358 7067 Fragments arranged by size: 7067 2010 1358 KpnI G_GTAC'C Cuts at: 7760 7760 Size: 10435 MfeI C'AATT_G Cuts at: 7173 8034 7173 Size: 861 9574 MluI A'CGCG_T Cuts at: 2277 10311 2277 Size: 8034 2401 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2099 2694 3021 6357 7533 9549 9733 1305 Size: 794 595 327 3336 1176 2016 184 2007 Fragments arranged by size: 3336 2016 2007 1176 794 595 327 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4775 7721 10 Size: 54 1425 343 2943 2946 2724 Fragments arranged by size: 2946 2943 2724 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 6824 8393 8752 8911 518 Size: 648 5658 1569 359 159 2042 Fragments arranged by size: 5658 2042 1569 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2139 2164 2251 3373 4399 5314 6284 6463 Size: 533 25 87 1122 1026 915 970 179 Cuts at: 6463 7851 8528 9676 9921 1606 Size: 1388 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 1122 1026 970 915 677 533 245 179 87 25 NcoI C'CATG_G Cuts at: 513 1065 2004 6689 513 Size: 552 939 4685 4259 Fragments arranged by size: 4685 4259 939 552 NdeI CA'TA_TG Cuts at: 387 7029 7306 387 Size: 6642 277 3516 Fragments arranged by size: 6642 3516 277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10435 NotI GC'GGCC_GC Cuts at: 7882 7882 Size: 10435 NsiI A_TGCA'T Cuts at: 2729 5210 6517 2729 Size: 2481 1307 6647 Fragments arranged by size: 6647 2481 1307 NspI r_CATG'y Cuts at: 1132 1229 5586 6730 7716 7793 9338 1132 Size: 97 4357 1144 986 77 1545 2229 Fragments arranged by size: 4357 2229 1545 1144 986 97 77 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10435 PciI A'CATG_T Cuts at: 5582 6726 9334 5582 Size: 1144 2608 6683 Fragments arranged by size: 6683 2608 1144 PflMI CCAn_nnn'nTGG Cuts at: 1108 6847 6919 1108 Size: 5739 72 4624 Fragments arranged by size: 5739 4624 72 PfoI T'CCnGG_A Cuts at: 5649 6846 7165 5649 Size: 1197 319 8919 Fragments arranged by size: 8919 1197 319 PmeI GTTT'AAAC Cuts at: 7739 7739 Size: 10435 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8504 8536 10017 10049 8504 Size: 32 1481 32 8890 Fragments arranged by size: 8890 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2303 4076 2303 Size: 1773 8662 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10435 PsiI TTA'TAA Cuts at: 8005 8005 Size: 10435 PstI C_TGCA'G Cuts at: 830 7787 830 Size: 6957 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10435 PvuII CAG'CTG Cuts at: 2251 4399 5314 6284 6463 2251 Size: 2148 915 970 179 6223 Fragments arranged by size: 6223 2148 970 915 179 SacI G_AGCT'C Cuts at: 2372 2603 5519 2372 Size: 231 2916 7288 Fragments arranged by size: 7288 2916 231 SacII CC_GC'GG Cuts at: 2165 2165 Size: 10435 SalI G'TCGA_C Cuts at: 1936 7777 1936 Size: 5841 4594 SanDI GG'GwC_CC Cuts at: 2303 2303 Size: 10435 SapI GCTCTTCn'nnn_ Cuts at: 2224 2591 3144 4743 2224 Size: 367 553 1599 7916 Fragments arranged by size: 7916 1599 553 367 SbfI CC_TGCA'GG Cuts at: 7787 7787 Size: 10435 ScaI AGT'ACT Cuts at: 5701 6116 8683 10395 5701 Size: 415 2567 1712 5741 Fragments arranged by size: 5741 2567 1712 415 SfcI C'TryA_G Cuts at: 826 1045 3279 5232 5373 5470 5931 7783 8918 Size: 219 2234 1953 141 97 461 1852 1135 Cuts at: 8918 9599 9790 826 Size: 681 191 1471 Fragments arranged by size: 2234 1953 1852 1471 1135 681 461 219 191 141 97 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10435 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10435 SmaI CCC'GGG Cuts at: 1872 2189 7762 1872 Size: 317 5573 4545 Fragments arranged by size: 5573 4545 317 SmlI C'TyrA_G Cuts at: 820 1017 1943 5177 6098 8539 9440 9702 820 Size: 197 926 3234 921 2441 901 262 1553 Fragments arranged by size: 3234 2441 1553 926 921 901 262 197 SnaBI TAC'GTA Cuts at: 493 6361 8226 493 Size: 5868 1865 2702 Fragments arranged by size: 5868 2702 1865 SpeI A'CTAG_T Cuts at: 152 152 Size: 10435 SphI G_CATG'C Cuts at: 1132 7793 1132 Size: 6661 3774 SspI AAT'ATT Cuts at: 5 52 6418 7162 8359 5 Size: 47 6366 744 1197 2081 Fragments arranged by size: 6366 2081 1197 744 47 StuI AGG'CCT Cuts at: 1517 2173 2452 6803 1517 Size: 656 279 4351 5149 Fragments arranged by size: 5149 4351 656 279 StyI C'CwwG_G Cuts at: 513 1065 2004 2424 4046 5496 6150 6252 6689 Size: 552 939 420 1622 1450 654 102 437 Cuts at: 6689 7581 7874 513 Size: 892 293 3074 Fragments arranged by size: 3074 1622 1450 939 892 654 552 437 420 293 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2483 8462 8479 8632 8817 9262 325 Size: 1255 903 5979 17 153 185 445 1498 Fragments arranged by size: 5979 1498 1255 903 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3223 3245 3456 Size: 274 80 33 51 1443 1246 22 211 Cuts at: 3456 5699 6085 6114 7345 8681 10393 96 Size: 2243 386 29 1231 1336 1712 138 Fragments arranged by size: 2243 1712 1443 1336 1246 1231 386 274 211 138 80 51 33 29 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3752 3784 10395 10427 3752 Size: 32 6611 32 3760 Fragments arranged by size: 6611 3760 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4080 1316 Size: 2764 7671 XbaI T'CTAG_A Cuts at: 7771 7771 Size: 10435 XcmI CCAnnnn_n'nnnnTGG Cuts at: 7729 7729 Size: 10435 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10435 XmaI C'CCGG_G Cuts at: 1870 2187 7760 1870 Size: 317 5573 4545 Fragments arranged by size: 5573 4545 317 XmnI GAAnn'nnTTC Cuts at: 4626 4644 4689 4873 5250 5505 8564 4626 Size: 18 45 184 377 255 3059 6497 Fragments arranged by size: 6497 3059 377 255 184 45 18 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8243 276 Size: 53 83 186 936 6709 2468 Fragments arranged by size: 6709 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI