Restriction map for
pFN21AA0541
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BbeI BbrPI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsiWI BspHI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FseI FspI Hin1I
HpaI KasI KpnI KspI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I RcaI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 4145 TCCGGA T^CCGGA
AseI 160 ATTAAT AT^TAAT
AsnI 160 ATTAAT AT^TAAT
Asp700I 1834 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 167 CTCGAG C^[CT]CG[GA]G
BamHI 3496 GGATCC G^GATCC
BbsI 4034 GTCTTC GAAGAC(2/6)
BbvCI 4315 CCTCAGC CCTCAGC(-5/-2)
BglI 3076 GCCGCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 4034 GTCTTC GAAGAC(2/6)
BsaBI 2878 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1425 GGTCTC GGTCTC(1/5)
BsaWI 4145 TCCGGA [AT]^CCGG[AT]
BseAI 4145 TCCGGA T^CCGGA
BsiEI 98 CGATCG CG[GA][CT]^CG
BsmBI 1899 GAGACG CGTCTC(1/5)
BsmI 2474 GAATGC GAATGC(1/-1)
BsoBI 167 CTCGAG C^[CT]CG[GA]G
BspDI 3053 ATCGAT AT^CGAT
BspEI 4145 TCCGGA T^CCGGA
BstAPI 3873 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 3053 ATCGAT AT^CGAT
DraIII 1376 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 122 GACAAATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2784 CCTTTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1653 ATGCAT ATGCA^T
HaeII 48 AGCGCC [GA]GCGC^[CT]
HindIII 215 AAGCTT A^AGCTT
MamI 2878 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4145 TCCGGA T^CCGGA
NsiI 1653 ATGCAT ATGCA^T
PaeR7I 167 CTCGAG C^TCGAG
PshBI 160 ATTAAT AT^TAAT
PvuI 98 CGATCG CGAT^CG
SacI 4112 GAGCTC GAGCT^C
SapI 3268 GAAGAGC GCTCTTC(1/4)
ScaI 2442 AGTACT AGT^ACT
SpeI 3487 ACTAGT A^CTAGT
XhoI 167 CTCGAG C^TCGAG
XmnI 1834 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 3210 CGCG CG^CG
4362 CGCG
AocI 3239 CCTCAGG CC^T[ACGT]AGG
3469 CCTGAGG
BalI 1360 TGGCCA TGG^CCA
3512 TGGCCA
BanI 4311 GGCACC G^G[CT][GA]CC
4344 GGTGCC
BcgI 3678 CGAACTTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3742 GCAGCTGACTCG
BciVI 597 GGATAC GTATCC(6/5)
1767 GGATAC
Bsp1407I 930 TGTACA T^GTACA
1238 TGTACA
BspLU11I 1402 ACATGT A^CATGT
2064 ACATGT
BsrGI 930 TGTACA T^GTACA
1238 TGTACA
BssSI 3899 CACGAG CACGAG(-5/-1)
3973 CTCGTG
BstUI 3210 CGCG CG^CG
4362 CGCG
Bsu36I 3239 CCTCAGG CC^T[ACGT]AGG
3469 CCTGAGG
Eco81I 3239 CCTCAGG CC^T[ACGT]AGG
3469 CCTGAGG
HgaI 2611 GCGTC GACGC(5/10)
4363 GCGTC
MluNI 1360 TGGCCA TGG^CCA
3512 TGGCCA
MscI 1360 TGGCCA TGG^CCA
3512 TGGCCA
MvnI 3210 CGCG CG^CG
4362 CGCG
NcoI 4119 CCATGG C^CATGG
4170 CCATGG
NdeI 2527 CATATG CA^TATG
2737 CATATG
PflMI 704 CCAAATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1246 CCAGCACATGG
PpuMI 753 AGGTCCT [GA]G^G[AT]CC[CT]
2748 GGGTCCT
PstI 4059 CTGCAG CTGCA^G
4193 CTGCAG
SexAI 1721 ACCTGGT A^CC[AT]GGT
4340 ACCAGGT
SmlI 167 CTCGAG C^T[CT][GA]AG
4387 CTCAAG
SphI 3768 GCATGC GCATG^C
4298 GCATGC
SspBI 930 TGTACA T^GTACA
1238 TGTACA
StuI 1705 AGGCCT AGG^CCT
3013 AGGCCT
Van91I 704 CCAAATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1246 CCAGCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AspI
AvaII BanII BbvI BclI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoRV EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII