(Circular) (Six-base) MAPSORT of: pFN21AA0519.seq Check: 6316 from: 1 to: 7462 pFN21AA0519 7462 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3209 3209 Size: 7462 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5272 278 Size: 53 83 186 936 3736 2468 Fragments arranged by size: 3736 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2592 4805 1937 Size: 655 2213 4594 Fragments arranged by size: 4594 2213 655 Acc65I G'GTAC_C Cuts at: 4783 4783 Size: 7462 AclI AA'CG_TT Cuts at: 1563 2679 5589 5962 1563 Size: 1116 2910 373 3063 Fragments arranged by size: 3063 2910 1116 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 3059 3173 3299 5525 6909 7177 1632 Size: 728 699 114 126 2226 1384 268 1917 Fragments arranged by size: 2226 1917 1384 728 699 268 126 114 AfeI AGC'GCT Cuts at: 3963 3963 Size: 7462 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7265 AflIII A'CryG_T Cuts at: 1203 6361 7338 1203 Size: 5158 977 1327 Fragments arranged by size: 5158 1327 977 AgeI A'CCGG_T Cuts at: 6293 6293 Size: 7462 AhdI GACnn_n'nnGTC Cuts at: 6191 6191 Size: 7462 AleI CACnn'nnGTG Cuts at: 1166 3998 1166 Size: 2832 4630 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7462 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3960 3992 3960 Size: 32 7430 AlwNI CAG_nnn'CTG Cuts at: 2386 3187 3905 4409 4600 5205 6777 2386 Size: 801 718 504 191 605 1572 3071 Fragments arranged by size: 3071 1572 801 718 605 504 191 ApaLI G'TGCA_C Cuts at: 5519 6675 5519 Size: 1156 6306 ApoI r'AATT_y Cuts at: 1493 3194 3887 3950 4093 4771 4997 5149 7258 Size: 1701 693 63 143 678 226 152 2109 Cuts at: 7258 1493 Size: 1697 Fragments arranged by size: 2109 1701 1697 693 678 226 152 143 63 AseI AT'TA_AT Cuts at: 160 1032 6016 160 Size: 872 4984 1606 Fragments arranged by size: 4984 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7462 AvaI C'yCGr_G Cuts at: 1870 1943 2205 2526 3311 3981 4296 4787 1870 Size: 73 262 321 785 670 315 491 4545 Fragments arranged by size: 4545 785 670 491 321 315 262 73 BamHI G'GATC_C Cuts at: 2635 4792 5159 2635 Size: 2157 367 4938 Fragments arranged by size: 4938 2157 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3007 3377 3457 4783 6238 Size: 325 217 627 1220 370 80 1326 1455 Cuts at: 6238 618 Size: 1842 Fragments arranged by size: 1842 1455 1326 1220 627 370 325 217 80 BanII G_rGCy'C Cuts at: 1391 2283 2439 2982 3899 4781 1391 Size: 892 156 543 917 882 4072 Fragments arranged by size: 4072 917 892 882 543 156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 928 Size: 971 220 6271 Fragments arranged by size: 6271 971 220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5653 5687 1903 Size: 34 3716 34 3678 Fragments arranged by size: 3716 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5354 6564 5354 Size: 1210 6252 BclI T'GATC_A Cuts at: 1548 1548 Size: 7462 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2800 2897 6073 Size: 107 122 71 631 605 1128 97 3176 Cuts at: 6073 136 Size: 1525 Fragments arranged by size: 3176 1525 1128 631 605 122 107 97 71 BglII A'GATC_T Cuts at: 7457 7457 Size: 7462 BlpI GC'TnA_GC Cuts at: 2099 2766 4879 2099 Size: 667 2113 4682 Fragments arranged by size: 4682 2113 667 Bme1580I G_kGCm'C Cuts at: 1790 3244 5523 6679 1790 Size: 1454 2279 1156 2573 Fragments arranged by size: 2573 2279 1454 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7462 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2896 5193 6151 Size: 416 53 83 399 24 1479 2297 958 Cuts at: 6151 442 Size: 1753 Fragments arranged by size: 2297 1753 1479 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7462 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3103 4311 3103 Size: 1208 6254 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2375 2792 3810 6122 1239 Size: 1073 63 417 1018 2312 2579 Fragments arranged by size: 2579 2312 1073 1018 417 63 Bpu10I CC'TnA_GC Cuts at: 3898 3898 Size: 7462 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2381 5587 6452 6750 2381 Size: 3206 865 298 3093 Fragments arranged by size: 3206 3093 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6125 882 Size: 617 4626 2219 Fragments arranged by size: 4626 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2094 2239 2719 2843 5253 493 Size: 711 890 145 480 124 2410 2702 Fragments arranged by size: 2702 2410 890 711 480 145 124 BsaBI GATnn'nnATC Cuts at: 7456 7456 Size: 7462 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4140 5269 5651 6286 Size: 53 83 186 936 2607 1129 382 635 Cuts at: 6286 275 Size: 1451 Fragments arranged by size: 2607 1451 1129 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3135 5895 6293 6567 6714 7125 3135 Size: 2760 398 274 147 411 3472 Fragments arranged by size: 3472 2760 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2765 2795 3960 3990 1178 Size: 30 165 30 1362 30 1165 30 4650 Fragments arranged by size: 4650 1362 1165 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7462 BseYI C'CCAG_C Cuts at: 3221 3394 3625 3932 6665 7161 3221 Size: 173 231 307 2733 496 3522 Fragments arranged by size: 3522 2733 496 307 231 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3182 1163 Size: 573 1446 5443 Fragments arranged by size: 5443 1446 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4912 5673 6701 1540 Size: 275 186 2911 761 1028 2301 Fragments arranged by size: 2911 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2283 2439 3826 4713 5523 5608 6679 2283 Size: 156 1387 887 810 85 1071 3066 Fragments arranged by size: 3066 1387 1071 887 810 156 85 BsmI GAATG_Cn' Cuts at: 2422 4973 5066 2422 Size: 2551 93 4818 Fragments arranged by size: 4818 2551 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3734 3734 Size: 7462 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2439 2982 3244 3826 3899 4713 Size: 399 493 156 543 262 582 73 814 Cuts at: 4713 4781 5523 5608 6679 1391 Size: 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 814 742 582 543 493 399 262 156 85 73 68 BspEI T'CCGG_A Cuts at: 7125 7125 Size: 7462 BspHI T'CATG_A Cuts at: 4606 5351 7081 4606 Size: 745 1730 4987 Fragments arranged by size: 4987 1730 745 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2066 2394 3209 3394 4817 844 Size: 1222 328 815 185 1423 3489 Fragments arranged by size: 3489 1423 1222 815 328 185 BsrBI CCG'CTC Cuts at: 1394 1823 2137 3087 3256 3632 4018 4630 5349 Size: 429 314 950 169 376 386 612 719 Cuts at: 5349 1394 Size: 3507 Fragments arranged by size: 3507 950 719 612 429 386 376 314 169 BsrDI GCAATG_nn' Cuts at: 65 1248 3141 3979 4503 5957 6131 65 Size: 1183 1893 838 524 1454 174 1396 Fragments arranged by size: 1893 1454 1396 1183 838 524 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2501 2535 2746 3010 6106 6293 1700 Size: 110 691 34 211 264 3096 187 2869 Fragments arranged by size: 3096 2869 691 264 211 187 110 34 BsrGI T'GTAC_A Cuts at: 96 4683 96 Size: 4587 2875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7462 BssSI C'ACGA_G Cuts at: 2670 3468 4625 5522 6534 2670 Size: 798 1157 897 1012 3598 Fragments arranged by size: 3598 1157 1012 897 798 BstAPI GCAn_nnn'nTGC Cuts at: 794 2386 794 Size: 1592 5870 BstBI TT'CG_AA Cuts at: 3093 3360 6266 3093 Size: 267 2906 4289 Fragments arranged by size: 4289 2906 267 BstEII G'GTnAC_C Cuts at: 1184 4555 1184 Size: 3371 4091 BstXI CCAn_nnnn'nTGG Cuts at: 2989 3892 4153 2989 Size: 903 261 6298 Fragments arranged by size: 6298 903 261 BstYI r'GATC_y Cuts at: 1972 2245 2635 3621 3660 4792 5159 5544 5561 Size: 273 390 986 39 1132 367 385 17 Cuts at: 5561 7013 7099 7111 7457 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1132 986 390 385 367 346 273 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 4593 4593 Size: 7462 BtgI C'CryG_G Cuts at: 513 1065 2892 4392 513 Size: 552 1827 1500 3583 Fragments arranged by size: 3583 1827 1500 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2125 2689 487 Size: 683 56 899 564 5260 Fragments arranged by size: 5260 899 683 564 56 BtsI GCAGTG_nn' Cuts at: 3440 3567 4978 5772 5792 3440 Size: 127 1411 794 20 5110 Fragments arranged by size: 5110 1411 794 127 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7462 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7427 DraI TTT'AAA Cuts at: 4766 4766 Size: 7462 DraIII CAC_nnn'GTG Cuts at: 3248 3248 Size: 7462 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2615 4139 6469 809 Size: 507 1299 1524 2330 1802 Fragments arranged by size: 2330 1802 1524 1299 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2884 4321 4909 Size: 54 1425 50 275 18 1054 1437 588 Cuts at: 4909 5798 7427 8 Size: 889 1629 43 Fragments arranged by size: 1629 1437 1425 1054 889 588 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4909 1537 Size: 275 3097 4090 Fragments arranged by size: 4090 3097 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 2354 3035 3090 3362 3639 4718 5392 Size: 918 83 681 55 272 277 1079 674 Cuts at: 5392 1353 Size: 3423 Fragments arranged by size: 3423 1079 918 681 674 277 272 83 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2075 2818 4241 6049 6423 6569 Size: 1064 138 486 743 1423 1808 374 146 Cuts at: 6569 387 Size: 1280 Fragments arranged by size: 1808 1423 1280 1064 743 486 374 146 138 EcoICRI GAG'CTC Cuts at: 2281 2437 2281 Size: 156 7306 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2360 2375 2792 3059 3173 3299 Size: 393 680 48 15 417 267 114 126 Cuts at: 3299 3810 5525 6122 6909 7177 1239 Size: 511 1715 597 787 268 1524 Fragments arranged by size: 1715 1524 787 680 597 511 417 393 268 267 126 114 48 15 EcoNI CCTnn'n_nnAGG Cuts at: 3077 4073 3077 Size: 996 6466 EcoO109I rG'GnC_Cy Cuts at: 2383 3184 3316 3718 3830 4117 4597 2383 Size: 801 132 402 112 287 480 5248 Fragments arranged by size: 5248 801 480 402 287 132 112 EcoRI G'AATT_C Cuts at: 3194 4093 4771 5149 3194 Size: 899 678 378 5507 Fragments arranged by size: 5507 899 678 378 FspI TGC'GCA Cuts at: 5968 5968 Size: 7462 HaeII r_GCGC'y Cuts at: 1678 2305 3965 6609 1678 Size: 627 1660 2644 2531 Fragments arranged by size: 2644 2531 1660 627 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2185 2217 3043 3075 3595 Size: 32 135 32 613 32 826 32 520 Cuts at: 3595 3627 6103 6135 6177 6209 1373 Size: 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 826 613 520 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4008 4806 5052 669 Size: 988 281 2070 798 246 3079 Fragments arranged by size: 3079 2070 988 798 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7462 HpaI GTT'AAC Cuts at: 5052 5052 Size: 7462 KpnI G_GTAC'C Cuts at: 4787 4787 Size: 7462 MfeI C'AATT_G Cuts at: 5061 5061 Size: 7462 MluI A'CGCG_T Cuts at: 7338 7338 Size: 7462 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 2500 4395 6576 6760 1305 Size: 795 400 1895 2181 184 2007 Fragments arranged by size: 2181 2007 1895 795 400 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5640 Fragments arranged by size: 5640 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2820 3998 4151 4508 4553 4576 5420 Size: 648 1654 1178 153 357 45 23 844 Cuts at: 5420 5779 5938 518 Size: 359 159 2042 Fragments arranged by size: 2042 1654 1178 844 648 359 357 159 153 45 23 MspA1I CmG'CkG Cuts at: 1606 2290 2959 3656 3813 4878 5555 6703 6948 Size: 684 669 697 157 1065 677 1148 245 Cuts at: 6948 1606 Size: 2120 Fragments arranged by size: 2120 1148 1065 697 684 677 669 245 157 NaeI GCC'GGC Cuts at: 2503 2537 2503 Size: 34 7428 NcoI C'CATG_G Cuts at: 513 1065 2892 4392 513 Size: 552 1827 1500 3583 Fragments arranged by size: 3583 1827 1500 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7462 NgoMIV G'CCGG_C Cuts at: 2501 2535 2501 Size: 34 7428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7462 NotI GC'GGCC_GC Cuts at: 4909 4909 Size: 7462 NspI r_CATG'y Cuts at: 1132 1229 2065 3430 4675 4820 6365 1132 Size: 97 836 1365 1245 145 1545 2229 Fragments arranged by size: 2229 1545 1365 1245 836 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7462 PciI A'CATG_T Cuts at: 6361 6361 Size: 7462 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7462 PfoI T'CCnGG_A Cuts at: 2259 3639 4467 2259 Size: 1380 828 5254 Fragments arranged by size: 5254 1380 828 PmeI GTTT'AAAC Cuts at: 4766 4766 Size: 7462 PmlI CAC'GTG Cuts at: 2094 2239 2094 Size: 145 7317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3960 3992 5531 5563 7044 7076 3960 Size: 32 1539 32 1481 32 4346 Fragments arranged by size: 4346 1539 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2383 3184 3316 3718 4117 2383 Size: 801 132 402 399 5728 Fragments arranged by size: 5728 801 402 399 132 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7462 PsiI TTA'TAA Cuts at: 5032 5032 Size: 7462 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4676 4708 4676 Size: 32 7430 PstI C_TGCA'G Cuts at: 830 2289 3435 4814 830 Size: 1459 1146 1379 3478 Fragments arranged by size: 3478 1459 1379 1146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7462 PvuII CAG'CTG Cuts at: 2290 3656 3813 2290 Size: 1366 157 5939 Fragments arranged by size: 5939 1366 157 SacI G_AGCT'C Cuts at: 2283 2439 2283 Size: 156 7306 SalI G'TCGA_C Cuts at: 1936 4804 1936 Size: 2868 4594 SanDI GG'GwC_CC Cuts at: 3316 3316 Size: 7462 SapI GCTCTTCn'nnn_ Cuts at: 2271 2354 3362 4718 2271 Size: 83 1008 1356 5015 Fragments arranged by size: 5015 1356 1008 83 SbfI CC_TGCA'GG Cuts at: 4814 4814 Size: 7462 ScaI AGT'ACT Cuts at: 2881 5710 7422 2881 Size: 2829 1712 2921 Fragments arranged by size: 2921 2829 1712 SfcI C'TryA_G Cuts at: 826 1045 2285 3431 4810 5945 6626 6817 826 Size: 219 1240 1146 1379 1135 681 191 1471 Fragments arranged by size: 1471 1379 1240 1146 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7462 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7462 SmaI CCC'GGG Cuts at: 1872 2207 3313 4789 1872 Size: 335 1106 1476 4545 Fragments arranged by size: 4545 1476 1106 335 SmlI C'TyrA_G Cuts at: 820 1017 1943 2396 5566 6467 6729 820 Size: 197 926 453 3170 901 262 1553 Fragments arranged by size: 3170 1553 926 901 453 262 197 SnaBI TAC'GTA Cuts at: 493 5253 493 Size: 4760 2702 SpeI A'CTAG_T Cuts at: 152 2725 152 Size: 2573 4889 SphI G_CATG'C Cuts at: 1132 2065 4820 1132 Size: 933 2755 3774 Fragments arranged by size: 3774 2755 933 SspI AAT'ATT Cuts at: 5 52 5386 5 Size: 47 5334 2081 Fragments arranged by size: 5334 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7462 StyI C'CwwG_G Cuts at: 513 1065 2640 2892 4392 4901 513 Size: 552 1575 252 1500 509 3074 Fragments arranged by size: 3074 1575 1500 552 509 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2432 3330 3778 5489 5506 5659 5844 Size: 1255 852 898 448 1711 17 153 185 Cuts at: 5844 6289 325 Size: 445 1498 Fragments arranged by size: 1711 1498 1255 898 852 448 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2879 2925 4683 Size: 274 80 33 51 1443 902 46 1758 Cuts at: 4683 5708 7420 96 Size: 1025 1712 138 Fragments arranged by size: 1758 1712 1443 1025 902 274 138 80 51 46 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2946 2978 4109 4141 7422 7454 2946 Size: 32 1131 32 3281 32 2954 Fragments arranged by size: 3281 2954 1131 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2945 1316 Size: 1629 5833 XbaI T'CTAG_A Cuts at: 4798 4798 Size: 7462 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2618 2930 4471 2618 Size: 312 1541 5609 Fragments arranged by size: 5609 1541 312 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7462 XmaI C'CCGG_G Cuts at: 1870 2205 3311 4787 1870 Size: 335 1106 1476 4545 Fragments arranged by size: 4545 1476 1106 335 XmnI GAAnn'nnTTC Cuts at: 5591 5591 Size: 7462 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5270 276 Size: 53 83 186 936 3736 2468 Fragments arranged by size: 3736 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI RsrII SacII SexAI SfiI SfoI SrfI SwaI