Restriction map for
pFN21AA0491
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
Hin1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MflI MluI MroI MslI MspA1I
MspI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 61 CGCG CG^CG
AciI 34 GCGG CCGC(-3/-1)
AflII 901 CTTAAG C^TTAAG
AlwNI 311 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 738 GGACC G^G[AT]CC
BalI 90 TGGCCA TGG^CCA
BbsI 102 GAAGAC GAAGAC(2/6)
BclI 992 TGATCA T^GATCA
BfrI 901 CTTAAG C^TTAAG
BpmI 742 CTCCAG CTGGAG(16/14)
BpuAI 102 GAAGAC GAAGAC(2/6)
BsgI 67 GTGCAG GTGCAG(16/14)
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsiYI 155 CCAAAATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 155 CCAAAATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BstAPI 544 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 61 CGCG CG^CG
CfoI 62 GCGC GCG^C
DsaI 848 CCATGG C^C[GA][CT]GG
EagI 35 CGGCCG C^GGCCG
EclXI 35 CGGCCG C^GGCCG
Eco52I 35 CGGCCG C^GGCCG
EcoT14I 848 CCATGG C^C[AT][AT]GG
FbaI 992 TGATCA T^GATCA
HgaI 40 GACGC GACGC(5/10)
HhaI 62 GCGC GCG^C
HinP1I 62 GCGC G^CGC
HindIII 85 AAGCTT A^AGCTT
MfeI 470 CAATTG C^AATTG
MluNI 90 TGGCCA TGG^CCA
MscI 90 TGGCCA TGG^CCA
MunI 470 CAATTG C^AATTG
MvnI 61 CGCG CG^CG
NcoI 848 CCATGG C^CATGG
NspI 657 ACATGC [GA]CATG^[CT]
PflMI 155 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 551 CTGCAG CTGCA^G
ScaI 961 AGTACT AGT^ACT
SfcI 551 CTGCAG C^T[GA][CT]AG
SmlI 901 CTTAAG C^T[CT][GA]AG
StyI 848 CCATGG C^C[AT][AT]GG
Van91I 155 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 372 CCAAAAAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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Asp700I 400 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1016 GAATGGATTC
BanI 46 GGCACC G^G[CT][GA]CC
1065 GGTGCC
BsmAI 504 GAGAC GTCTC(1/5)
531 GAGAC
BsmFI 291 GGGAC GGGAC(10/14)
313 GGGAC
Cac8I 30 GCTGGC GC[ACGT]^[ACGT]GC
43 GCAGGC
Cfr13I 325 GGCCC G^G[ACGT]CC
738 GGACC
DpnII 607 GATC ^GATC
993 GATC
EaeI 35 CGGCCG [CT]^GGCC[GA]
90 TGGCCA
MaeII 20 ACGT A^CGT
412 ACGT
MboI 607 GATC ^GATC
993 GATC
MwoI 34 GCGGCCGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
544 GCAAAAGCTGC
NdeII 607 GATC ^GATC
993 GATC
NlaIV 46 GGCACC GG[ACGT]^[ACGT]CC
1065 GGTGCC
Sau3AI 607 GATC ^GATC
993 GATC
Sau96I 325 GGCCC G^G[ACGT]CC
738 GGACC
Tsp45I 802 GTGAC ^GT[GC]AC
896 GTGAC
XmnI 400 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1016 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BsrI BssKI BstF5I
BstNI DdeI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboII MnlI MseI MvaI
NlaIII RsaI ScrFI SfaNI TfiI
Tru9I TseI Tsp509I TspRI