Restriction map for
pFN21AA0481
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BciVI BclI
BfaI BfrI BglII BlnI BsaBI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FbaI FseI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MunI NdeI NheI
NotI NspV PacI PaeR7I PflMI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SalI ScaI SfiI
SfuI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tsp509I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 300 GACGTC GACGT^C
AccIII 378 TCCGGA T^CCGGA
AocI 1360 CCTGAGG CC^T[ACGT]AGG
AspI 742 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2110 CACGTG CAC^GTG
BbvCI 718 GCTGAGG CCTCAGC(-5/-2)
BcgI 918 GCAGGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1454 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1454 GCTCAGC GC^T[ACGT]AGC
BsaAI 2110 CACGTG [CT]AC^GT[GA]
BseAI 378 TCCGGA T^CCGGA
BsmI 1905 GAATGC GAATGC(1/-1)
BspEI 378 TCCGGA T^CCGGA
BspHI 2012 TCATGA T^CATGA
BssHII 1173 GCGCGC G^CGCGC
BstEII 1845 GGTCACC G^GT[ACGT]ACC
BstPI 1845 GGTCACC G^GT[ACGT]ACC
Bsu36I 1360 CCTGAGG CC^T[ACGT]AGG
CelII 1454 GCTCAGC GC^T[ACGT]AGC
DraI 201 TTTAAA TTT^AAA
Eco81I 1360 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1845 GGTCACC G^GT[ACGT]ACC
EcoT22I 385 ATGCAT ATGCA^T
KspI 161 CCGCGG CCGC^GG
MroI 378 TCCGGA T^CCGGA
MseI 202 TTAA T^TAA
NruI 927 TCGCGA TCG^CGA
NsiI 385 ATGCAT ATGCA^T
NspI 1136 ACATGC [GA]CATG^[CT]
PflFI 742 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2110 CACGTG CAC^GTG
PmlI 2110 CACGTG CAC^GTG
PshAI 1839 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2012 TCATGA T^CATGA
SacII 161 CCGCGG CCGC^GG
SbfI 1662 CCTGCAGG CCTGCA^GG
SexAI 761 ACCTGGT A^CC[AT]GGT
Sse8387I 1662 CCTGCAGG CCTGCA^GG
TfiI 310 GATTC G^A[AT]TC
Tru9I 202 TTAA T^TAA
Tth111I 742 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 704 ACGTGT A^C[GA][CT]GT
2111 ACGTGT
Aor51HI 371 AGCGCT AGC^GCT
1673 AGCGCT
ApaI 1102 GGGCCC GGGCC^C
1354 GGGCCC
AviII 593 TGCGCA TGC^GCA
2027 TGCGCA
BsaWI 378 TCCGGA [AT]^CCGG[AT]
837 ACCGGA
BstAPI 414 GCACGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2109 GCACGTGTTGC
BstXI 809 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2042 CCACCCTCCTGG
BstYI 461 AGATCC [GA]^GATC[CT]
884 AGATCC
Eco47III 371 AGCGCT AGC^GCT
1673 AGCGCT
FspI 593 TGCGCA TGC^GCA
2027 TGCGCA
MflI 461 AGATCC [GA]^GATC[CT]
884 AGATCC
NcoI 789 CCATGG C^CATGG
1607 CCATGG
PpuMI 177 AGGTCCC [GA]G^G[AT]CC[CT]
1337 AGGACCC
SapI 1561 GAAGAGC GCTCTTC(1/4)
656 GCTCTTC
SgrAI 150 CGCCGGCG C[GA]^CCGG[CT]G
1072 CGCCGGCG
TaqI 749 TCGA T^CGA
1007 TCGA
TthHB8I 749 TCGA T^CGA
1007 TCGA
XhoII 461 AGATCC [GA]^GATC[CT]
884 AGATCC
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TseI
Tsp45I TspRI XmaCI XmaI