Restriction map for
pFN21AA0481
(Linear) (Six-base) MAPSORT of: KIAA0481.seq Check: 5744 from: 1 to: 2127
KIAA0481.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 89 596 881 972 1869 2127
Size: 89 507 285 91 897 258
Fragments arranged by size:
897 507 285 258 91 89
AatII G_ACGT'C
Cuts at: 0 304 2127
Size: 304 1823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 271 910 1102 1171 1354 1639 1768
Size: 128 143 639 192 69 183 285 129
Cuts at: 1768 2127
Size: 359
Fragments arranged by size:
639 359 285 192 183 143 129 128
69
AfeI AGC'GCT
Cuts at: 0 373 1675 2127
Size: 373 1302 452
Fragments arranged by size:
1302 452 373
AflIII A'CryG_T
Cuts at: 0 704 2111 2127
Size: 704 1407 16
Fragments arranged by size:
1407 704 16
AleI CACnn'nnGTG
Cuts at: 0 1258 2127
Size: 1258 869
AlwNI CAG_nnn'CTG
Cuts at: 0 219 1149 1662 1758 2127
Size: 219 930 513 96 369
Fragments arranged by size:
930 513 369 219 96
ApaI G_GGCC'C
Cuts at: 0 1106 1358 2127
Size: 1106 252 769
Fragments arranged by size:
1106 769 252
AvaI C'yCGr_G
Cuts at: 0 281 1274 1373 1841 2127
Size: 281 993 99 468 286
Fragments arranged by size:
993 468 286 281 99
BanI G'GyrC_C
Cuts at: 0 165 490 834 1076 1162 2127
Size: 165 325 344 242 86 965
Fragments arranged by size:
965 344 325 242 165 86
BanII G_rGCy'C
Cuts at: 0 232 569 1106 1358 1510 2127
Size: 232 337 537 252 152 617
Fragments arranged by size:
617 537 337 252 232 152
BbeI G_GCGC'C
Cuts at: 0 169 494 1080 1166 2127
Size: 169 325 586 86 961
Fragments arranged by size:
961 586 325 169 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 1619 1627 2023 2127
Size: 1619 8 396 104
Fragments arranged by size:
1619 396 104 8
BbvCI CC'TCA_GC
Cuts at: 0 719 2127
Size: 719 1408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 907 941 2127
Size: 907 34 1186
Fragments arranged by size:
1186 907 34
BfrBI ATG'CAT
Cuts at: 0 387 2127
Size: 387 1740
BglI GCCn_nnn'nGGC
Cuts at: 0 429 1110 1232 2127
Size: 429 681 122 895
Fragments arranged by size:
895 681 429 122
BlpI GC'TnA_GC
Cuts at: 0 1455 2127
Size: 1455 672
Bme1580I G_kGCm'C
Cuts at: 0 827 837 1106 1358 1380 1806 2127
Size: 827 10 269 252 22 426 321
Fragments arranged by size:
827 426 321 269 252 22 10
BmgBI CAC'GTC
Cuts at: 0 822 1125 1158 2127
Size: 822 303 33 969
Fragments arranged by size:
969 822 303 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 1650 2091 2127
Size: 1650 441 36
Fragments arranged by size:
1650 441 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1081 1389 1422 1618 1714 1876 1912
Size: 583 498 308 33 196 96 162 36
Cuts at: 1912 2125 2127
Size: 213 2
Fragments arranged by size:
583 498 308 213 196 162 96 36
33 2
Bpu10I CC'TnA_GC
Cuts at: 0 719 772 2127
Size: 719 53 1355
Fragments arranged by size:
1355 719 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 284 752 2127
Size: 103 181 468 1375
Fragments arranged by size:
1375 468 181 103
BsaAI yAC'GTr
Cuts at: 0 2112 2127
Size: 2112 15
BsaHI Gr'CG_yC
Cuts at: 0 149 166 301 491 1077 1163 1214 2127
Size: 149 17 135 190 586 86 51 913
Fragments arranged by size:
913 586 190 149 135 86 51 17
BsaWI w'CCGG_w
Cuts at: 0 378 837 2127
Size: 378 459 1290
Fragments arranged by size:
1290 459 378
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 749 779 1573 1603 2071 2098
Size: 337 30 382 30 794 30 468 27
Cuts at: 2098 2101 2127
Size: 3 26
Fragments arranged by size:
794 468 382 337 30 30 30 27
26 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 736 1378 1684 1711 1786 2059 2127
Size: 58 678 642 306 27 75 273 68
Fragments arranged by size:
678 642 306 273 75 68 58 27
BseYI C'CCAG_C
Cuts at: 0 55 314 569 845 1043 1483 1500 1926
Size: 55 259 255 276 198 440 17 426
Cuts at: 1926 2122 2127
Size: 196 5
Fragments arranged by size:
440 426 276 259 255 198 196 55
17 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 397 484 616 859 1033 2127
Size: 30 367 87 132 243 174 1094
Fragments arranged by size:
1094 367 243 174 132 87 30
BsiHKAI G_wGCw'C
Cuts at: 0 296 1067 1438 1536 2111 2127
Size: 296 771 371 98 575 16
Fragments arranged by size:
771 575 371 296 98 16
BsmI GAATG_Cn'
Cuts at: 0 1911 2127
Size: 1911 216
BsmBI CGTCTCn'nnnn_
Cuts at: 0 308 399 1407 2127
Size: 308 91 1008 720
Fragments arranged by size:
1008 720 308 91
Bsp1286I G_dGCh'C
Cuts at: 0 232 296 569 827 837 1067 1106 1358
Size: 232 64 273 258 10 230 39 252
Cuts at: 1358 1380 1438 1510 1536 1806 2111 2127
Size: 22 58 72 26 270 305 16
Fragments arranged by size:
305 273 270 258 252 232 230 72
64 58 39 26 22 16 10
BspEI T'CCGG_A
Cuts at: 0 378 2127
Size: 378 1749
BspHI T'CATG_A
Cuts at: 0 2012 2127
Size: 2012 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 428 596 881 972 1869 2127
Size: 89 339 168 285 91 897 258
Fragments arranged by size:
897 339 285 258 168 91 89
BsrBI CCG'CTC
Cuts at: 0 467 656 1385 1689 2127
Size: 467 189 729 304 438
Fragments arranged by size:
729 467 438 304 189
BsrFI r'CCGG_y
Cuts at: 0 151 539 784 1073 2127
Size: 151 388 245 289 1054
Fragments arranged by size:
1054 388 289 245 151
BssHII G'CGCG_C
Cuts at: 0 1173 2127
Size: 1173 954
BstAPI GCAn_nnn'nTGC
Cuts at: 0 420 2115 2127
Size: 420 1695 12
Fragments arranged by size:
1695 420 12
BstEII G'GTnAC_C
Cuts at: 0 1845 2127
Size: 1845 282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 816 2049 2127
Size: 816 1233 78
Fragments arranged by size:
1233 816 78
BstYI r'GATC_y
Cuts at: 0 461 884 2127
Size: 461 423 1243
Fragments arranged by size:
1243 461 423
Bsu36I CC'TnA_GG
Cuts at: 0 1361 2127
Size: 1361 766
BtgI C'CryG_G
Cuts at: 0 113 161 243 789 1259 1607 1823 2127
Size: 113 48 82 546 470 348 216 304
Fragments arranged by size:
546 470 348 304 216 113 82 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 373 1023 1308 1439 1974 2127
Size: 373 650 285 131 535 153
Fragments arranged by size:
650 535 373 285 153 131
BtsI GCAGTG_nn'
Cuts at: 0 516 674 1317 1395 2127
Size: 516 158 643 78 732
Fragments arranged by size:
732 643 516 158 78
DraI TTT'AAA
Cuts at: 0 203 2127
Size: 203 1924
EaeI y'GGCC_r
Cuts at: 0 240 620 689 786 959 1760 1961 2127
Size: 240 380 69 97 173 801 201 166
Fragments arranged by size:
801 380 240 201 173 166 97 69
EarI CTCTTCn'nnn_
Cuts at: 0 663 1556 2127
Size: 663 893 571
Fragments arranged by size:
893 663 571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 271 583 910 1081 1102 1171 1354
Size: 128 143 312 327 171 21 69 183
Cuts at: 1354 1389 1422 1618 1639 1714 1768 1876 1912
Size: 35 33 196 21 75 54 108 36
Cuts at: 1912 2125 2127
Size: 213 2
Fragments arranged by size:
327 312 213 196 183 171 143 128
108 75 69 54 36 35 33 21
21 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 718 1240 1666 1750 1797 2127
Size: 718 522 426 84 47 330
Fragments arranged by size:
718 522 426 330 84 47
EcoO109I rG'GnC_Cy
Cuts at: 0 178 600 1103 1338 1355 2127
Size: 178 422 503 235 17 772
Fragments arranged by size:
772 503 422 235 178 17
FspI TGC'GCA
Cuts at: 0 595 2029 2127
Size: 595 1434 98
Fragments arranged by size:
1434 595 98
HaeII r_GCGC'y
Cuts at: 0 158 169 375 494 1080 1166 1445 1451
Size: 158 11 206 119 586 86 279 6
Cuts at: 1451 1501 1677 1924 1957 2127
Size: 50 176 247 33 170
Fragments arranged by size:
586 279 247 206 176 170 158 119
86 50 33 11 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 1420 1452 1573 1605 2127
Size: 337 32 1051 32 121 32 522
Fragments arranged by size:
1051 522 337 121 32 32 32
KasI G'GCGC_C
Cuts at: 0 165 490 1076 1162 2127
Size: 165 325 586 86 965
Fragments arranged by size:
965 586 325 165 86
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 2127
Size: 368 1759
MscI TGG'CCA
Cuts at: 0 242 961 1762 2127
Size: 242 719 801 365
Fragments arranged by size:
801 719 365 242
MslI CAynn'nnrTG
Cuts at: 0 1258 1648 1942 1963 2127
Size: 1258 390 294 21 164
Fragments arranged by size:
1258 390 294 164 21
MspA1I CmG'CkG
Cuts at: 0 163 219 527 849 1047 1187 1629 1890
Size: 163 56 308 322 198 140 442 261
Cuts at: 1890 2127
Size: 237
Fragments arranged by size:
442 322 308 261 237 198 163 140
56
NaeI GCC'GGC
Cuts at: 0 153 541 786 1075 2127
Size: 153 388 245 289 1052
Fragments arranged by size:
1052 388 289 245 153
NarI GG'CG_CC
Cuts at: 0 166 491 1077 1163 2127
Size: 166 325 586 86 964
Fragments arranged by size:
964 586 325 166 86
NcoI C'CATG_G
Cuts at: 0 789 1607 2127
Size: 789 818 520
Fragments arranged by size:
818 789 520
NgoMIV G'CCGG_C
Cuts at: 0 151 539 784 1073 2127
Size: 151 388 245 289 1054
Fragments arranged by size:
1054 388 289 245 151
NruI TCG'CGA
Cuts at: 0 929 2127
Size: 929 1198
NsiI A_TGCA'T
Cuts at: 0 389 2127
Size: 389 1738
NspI r_CATG'y
Cuts at: 0 1140 2127
Size: 1140 987
PfoI T'CCnGG_A
Cuts at: 0 1536 1814 2102 2127
Size: 1536 278 288 25
Fragments arranged by size:
1536 288 278 25
PmlI CAC'GTG
Cuts at: 0 2112 2127
Size: 2112 15
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1090 1122 2127
Size: 1090 32 1005
Fragments arranged by size:
1090 1005 32
PpuMI rG'GwC_Cy
Cuts at: 0 178 1338 2127
Size: 178 1160 789
Fragments arranged by size:
1160 789 178
PshAI GACnn'nnGTC
Cuts at: 0 1843 2127
Size: 1843 284
PspOMI G'GGCC_C
Cuts at: 0 1102 1354 2127
Size: 1102 252 773
Fragments arranged by size:
1102 773 252
PstI C_TGCA'G
Cuts at: 0 29 458 1634 1667 1865 2127
Size: 29 429 1176 33 198 262
Fragments arranged by size:
1176 429 262 198 33 29
PvuII CAG'CTG
Cuts at: 0 219 1047 1629 1890 2127
Size: 219 828 582 261 237
Fragments arranged by size:
828 582 261 237 219
SacII CC_GC'GG
Cuts at: 0 164 2127
Size: 164 1963
SapI GCTCTTCn'nnn_
Cuts at: 0 663 1556 2127
Size: 663 893 571
Fragments arranged by size:
893 663 571
SbfI CC_TGCA'GG
Cuts at: 0 1667 2127
Size: 1667 460
SexAI A'CCwGG_T
Cuts at: 0 761 2127
Size: 761 1366
SfcI C'TryA_G
Cuts at: 0 25 454 1630 1663 1677 1861 2127
Size: 25 429 1176 33 14 184 266
Fragments arranged by size:
1176 429 266 184 33 25 14
SfoI GGC'GCC
Cuts at: 0 167 492 1078 1164 2127
Size: 167 325 586 86 963
Fragments arranged by size:
963 586 325 167 86
SgrAI Cr'CCGG_yG
Cuts at: 0 151 1073 2127
Size: 151 922 1054
Fragments arranged by size:
1054 922 151
SmaI CCC'GGG
Cuts at: 0 1276 1375 1843 2127
Size: 1276 99 468 284
Fragments arranged by size:
1276 468 284 99
SrfI GCCC'GGGC
Cuts at: 0 1375 2127
Size: 1375 752
StyI C'CwwG_G
Cuts at: 0 789 854 1118 1607 2127
Size: 789 65 264 489 520
Fragments arranged by size:
789 520 489 264 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 266 1264 2127
Size: 266 998 863
Fragments arranged by size:
998 863 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 749 781 1796 1828 2071 2103 2127
Size: 749 32 1015 32 243 32 24
Fragments arranged by size:
1015 749 243 32 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 745 2127
Size: 745 1382
XmaI C'CCGG_G
Cuts at: 0 1274 1373 1841 2127
Size: 1274 99 468 286
Fragments arranged by size:
1274 468 286 99
ZraI GAC'GTC
Cuts at: 0 302 2127
Size: 302 1825
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NsiI NspI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuII SacII SapI
SbfI SexAI SfcI SfoI SgrAI SmaI SrfI StyI
TaqII TstI Tth111I XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BglII BmtI BplI BpuEI BsaBI BsiEI
BsiWI BsrDI BsrGI BssSI BstBI BstZ17I ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI PacI PasI PciI PflMI PmeI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
ScaI SfiI SgfI SmlI SnaBI SpeI SphI SspI
StuI SwaI TatI XbaI XcmI XhoI XmnI