(Circular) (Six-base) MAPSORT of: pFN21AA0472.seq Check: 6001 from: 1 to: 7492 pFN21AA0472 7492 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2398 3685 2398 Size: 1287 6205 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3333 5302 278 Size: 53 83 186 936 1797 1969 2468 Fragments arranged by size: 2468 1969 1797 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4002 4497 4835 1937 Size: 2065 495 338 4594 Fragments arranged by size: 4594 2065 495 338 Acc65I G'GTAC_C Cuts at: 4813 4813 Size: 7492 AclI AA'CG_TT Cuts at: 1563 5619 5992 1563 Size: 4056 373 3063 Fragments arranged by size: 4056 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2783 4259 5555 6939 7207 1632 Size: 1151 1476 1296 1384 268 1917 Fragments arranged by size: 1917 1476 1384 1296 1151 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7295 AflIII A'CryG_T Cuts at: 1203 4262 6391 7368 1203 Size: 3059 2129 977 1327 Fragments arranged by size: 3059 2129 1327 977 AgeI A'CCGG_T Cuts at: 6323 6323 Size: 7492 AhdI GACnn_n'nnGTC Cuts at: 6221 6221 Size: 7492 AleI CACnn'nnGTG Cuts at: 1166 3665 1166 Size: 2499 4993 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4208 4389 1184 Size: 3024 181 4287 Fragments arranged by size: 4287 3024 181 AlwNI CAG_nnn'CTG Cuts at: 2137 2955 3508 3976 4538 5235 6807 2137 Size: 818 553 468 562 697 1572 2822 Fragments arranged by size: 2822 1572 818 697 562 553 468 ApaLI G'TGCA_C Cuts at: 2525 3098 5549 6705 2525 Size: 573 2451 1156 3312 Fragments arranged by size: 3312 2451 1156 573 ApoI r'AATT_y Cuts at: 1493 4094 4509 4801 5027 5179 7288 1493 Size: 2601 415 292 226 152 2109 1697 Fragments arranged by size: 2601 2109 1697 415 292 226 152 AseI AT'TA_AT Cuts at: 160 1032 6046 160 Size: 872 5014 1606 Fragments arranged by size: 5014 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7492 AvaI C'yCGr_G Cuts at: 1870 1943 2044 2487 4817 1870 Size: 73 101 443 2330 4545 Fragments arranged by size: 4545 2330 443 101 73 BamHI G'GATC_C Cuts at: 4822 5189 4822 Size: 367 7125 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2457 3362 4813 6268 Size: 325 217 627 233 437 905 1451 1455 Cuts at: 6268 618 Size: 1842 Fragments arranged by size: 1842 1455 1451 905 627 437 325 233 217 BanII G_rGCy'C Cuts at: 1391 2040 2861 2971 3674 4612 4811 1391 Size: 649 821 110 703 938 199 4072 Fragments arranged by size: 4072 938 821 703 649 199 110 BbeI G_GCGC'C Cuts at: 2461 2461 Size: 7492 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4616 928 Size: 971 2717 3804 Fragments arranged by size: 3804 2717 971 BbvCI CC'TCA_GC Cuts at: 3731 3872 3731 Size: 141 7351 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3785 3819 5683 5717 1903 Size: 34 1848 34 1864 34 3678 Fragments arranged by size: 3678 1864 1848 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2974 5384 6594 2974 Size: 2410 1210 3872 Fragments arranged by size: 3872 2410 1210 BclI T'GATC_A Cuts at: 1548 3444 1548 Size: 1896 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2061 2238 3840 Size: 107 122 71 631 605 389 177 1602 Cuts at: 3840 6103 136 Size: 2263 1525 Fragments arranged by size: 2263 1602 1525 631 605 389 177 122 107 71 BglII A'GATC_T Cuts at: 3561 3862 7487 3561 Size: 301 3625 3566 Fragments arranged by size: 3625 3566 301 BlpI GC'TnA_GC Cuts at: 4909 4909 Size: 7492 Bme1580I G_kGCm'C Cuts at: 1790 2529 3102 4042 5553 6709 1790 Size: 739 573 940 1511 1156 2573 Fragments arranged by size: 2573 1511 1156 940 739 573 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7492 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2563 2864 3974 Size: 416 53 83 399 24 1146 301 1110 Cuts at: 3974 3986 4027 5223 6181 442 Size: 12 41 1196 958 1753 Fragments arranged by size: 1753 1196 1146 1110 958 416 399 301 83 53 41 24 12 BmtI G_CTAG'C Cuts at: 1056 4575 1056 Size: 3519 3973 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2059 4626 2059 Size: 2567 4925 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2600 2648 3530 4044 4165 4277 4706 Size: 1061 300 48 882 514 121 112 429 Cuts at: 4706 6152 1239 Size: 1446 2579 Fragments arranged by size: 2579 1446 1061 882 514 429 300 121 112 48 Bpu10I CC'TnA_GC Cuts at: 3731 3872 4531 3731 Size: 141 659 6692 Fragments arranged by size: 6692 659 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3052 5617 6482 6780 3052 Size: 2565 865 298 3764 Fragments arranged by size: 3764 2565 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2138 2552 4116 6155 882 Size: 617 639 414 1564 2039 2219 Fragments arranged by size: 2219 2039 1564 639 617 414 BsaAI yAC'GTr Cuts at: 493 1204 5283 493 Size: 711 4079 2702 Fragments arranged by size: 4079 2702 711 BsaBI GATnn'nnATC Cuts at: 7486 7486 Size: 7492 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2458 3330 5299 5681 Size: 53 83 186 936 925 872 1969 382 Cuts at: 5681 6316 275 Size: 635 1451 Fragments arranged by size: 1969 1451 936 925 872 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3771 5925 6323 6597 6744 7155 3771 Size: 2154 398 274 147 411 4108 Fragments arranged by size: 4108 2154 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3188 3218 3342 3372 1178 Size: 30 165 30 1785 30 124 30 5298 Fragments arranged by size: 5298 1785 165 124 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2188 2453 2585 2866 4187 4655 1186 Size: 1002 265 132 281 1321 468 4023 Fragments arranged by size: 4023 1321 1002 468 281 265 132 BseYI C'CCAG_C Cuts at: 2428 4244 6695 7191 2428 Size: 1816 2451 496 2729 Fragments arranged by size: 2729 2451 1816 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2426 4292 1163 Size: 573 690 1866 4363 Fragments arranged by size: 4363 1866 690 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2101 4942 5703 6731 1540 Size: 275 186 100 2841 761 1028 2301 Fragments arranged by size: 2841 2301 1028 761 275 186 100 BsiHKAI G_wGCw'C Cuts at: 2177 2529 3038 3102 4197 5553 5638 6709 2177 Size: 352 509 64 1095 1356 85 1071 2960 Fragments arranged by size: 2960 1356 1095 1071 509 352 85 64 BsmI GAATG_Cn' Cuts at: 5003 5096 5003 Size: 93 7399 BsmBI CGTCTCn'nnnn_ Cuts at: 2047 2047 Size: 7492 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2177 2529 2861 2971 3038 3102 Size: 399 250 137 352 332 110 67 64 Cuts at: 3102 3674 4042 4197 4612 4811 5553 5638 6709 Size: 572 368 155 415 199 742 85 1071 Cuts at: 6709 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 572 415 399 368 352 332 250 199 155 137 110 85 67 64 BspEI T'CCGG_A Cuts at: 3771 7155 3771 Size: 3384 4108 BspHI T'CATG_A Cuts at: 4731 5381 7111 4731 Size: 650 1730 5112 Fragments arranged by size: 5112 1730 650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2145 2398 3162 3685 4847 844 Size: 1301 253 764 523 1162 3489 Fragments arranged by size: 3489 1301 1162 764 523 253 BsrBI CCG'CTC Cuts at: 1394 1823 4210 5379 1394 Size: 429 2387 1169 3507 Fragments arranged by size: 3507 2387 1169 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4185 5987 6161 65 Size: 1183 2937 1802 174 1396 Fragments arranged by size: 2937 1802 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3843 6136 6323 1700 Size: 110 2033 2293 187 2869 Fragments arranged by size: 2869 2293 2033 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7492 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7492 BssSI C'ACGA_G Cuts at: 3794 5552 6564 3794 Size: 1758 1012 4722 Fragments arranged by size: 4722 1758 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7492 BstBI TT'CG_AA Cuts at: 6296 6296 Size: 7492 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7492 BstXI CCAn_nnnn'nTGG Cuts at: 2428 2890 3175 3688 2428 Size: 462 285 513 6232 Fragments arranged by size: 6232 513 462 285 BstYI r'GATC_y Cuts at: 1972 2575 3393 3561 3862 4138 4222 4822 5189 Size: 603 818 168 301 276 84 600 367 Cuts at: 5189 5574 5591 7043 7129 7141 7487 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 818 603 600 385 367 346 301 276 168 86 84 17 12 BstZ17I GTA'TAC Cuts at: 4003 4003 Size: 7492 Bsu36I CC'TnA_GG Cuts at: 2298 2568 3464 4551 2298 Size: 270 896 1087 5239 Fragments arranged by size: 5239 1087 896 270 BtgI C'CryG_G Cuts at: 513 1065 2004 2024 4144 4488 513 Size: 552 939 20 2120 344 3517 Fragments arranged by size: 3517 2120 939 552 344 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2256 487 Size: 683 56 1030 5723 Fragments arranged by size: 5723 1030 683 56 BtsI GCAGTG_nn' Cuts at: 2440 3100 3106 3435 5008 5802 5822 2440 Size: 660 6 329 1573 794 20 4110 Fragments arranged by size: 4110 1573 794 660 329 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7492 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4153 4188 529 Size: 35 3589 35 3833 Fragments arranged by size: 3833 3589 35 35 DraI TTT'AAA Cuts at: 3325 4796 3325 Size: 1471 6021 DraIII CAC_nnn'GTG Cuts at: 3052 3052 Size: 7492 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3335 6499 809 Size: 507 2019 3164 1802 Fragments arranged by size: 3164 2019 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2356 2974 Size: 54 1425 50 275 18 268 258 618 Cuts at: 2974 3832 4939 5828 7457 8 Size: 858 1107 889 1629 43 Fragments arranged by size: 1629 1425 1107 889 858 618 275 268 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2098 4939 1537 Size: 275 286 2841 4090 Fragments arranged by size: 4090 2841 286 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2298 3692 4682 5422 1353 Size: 945 1394 990 740 3423 Fragments arranged by size: 3423 1394 990 945 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2082 6079 6453 6599 387 Size: 1064 138 493 3997 374 146 1280 Fragments arranged by size: 3997 1280 1064 493 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2600 2648 2783 3530 4044 4165 Size: 393 668 300 48 135 747 514 121 Cuts at: 4165 4259 4277 4706 5555 6152 6939 7207 1239 Size: 94 18 429 849 597 787 268 1524 Fragments arranged by size: 1524 849 787 747 668 597 514 429 393 300 268 135 121 94 48 18 EcoNI CCTnn'n_nnAGG Cuts at: 2357 2881 4687 2357 Size: 524 1806 5162 Fragments arranged by size: 5162 1806 524 EcoO109I rG'GnC_Cy Cuts at: 2048 3085 3151 2048 Size: 1037 66 6389 Fragments arranged by size: 6389 1037 66 EcoRI G'AATT_C Cuts at: 4509 4801 5179 4509 Size: 292 378 6822 Fragments arranged by size: 6822 378 292 EcoRV GAT'ATC Cuts at: 4336 4336 Size: 7492 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3945 3945 Size: 7492 FspI TGC'GCA Cuts at: 5998 5998 Size: 7492 HaeII r_GCGC'y Cuts at: 1678 2461 2504 3738 6639 1678 Size: 783 43 1234 2901 2531 Fragments arranged by size: 2901 2531 1234 783 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2470 2502 2759 2791 2935 Size: 32 135 32 898 32 257 32 144 Cuts at: 2935 2967 6133 6165 6207 6239 1373 Size: 32 3166 32 42 32 2626 Fragments arranged by size: 3166 2626 898 257 144 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4836 5082 669 Size: 988 281 2898 246 3079 Fragments arranged by size: 3079 2898 988 281 246 HindIII A'AGCT_T Cuts at: 748 2450 3472 748 Size: 1702 1022 4768 Fragments arranged by size: 4768 1702 1022 HpaI GTT'AAC Cuts at: 5082 5082 Size: 7492 KasI G'GCGC_C Cuts at: 2457 2457 Size: 7492 KpnI G_GTAC'C Cuts at: 4817 4817 Size: 7492 MfeI C'AATT_G Cuts at: 5091 5091 Size: 7492 MluI A'CGCG_T Cuts at: 7368 7368 Size: 7492 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3412 3460 6606 6790 1305 Size: 2107 48 3146 184 2007 Fragments arranged by size: 3146 2107 2007 184 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 2976 3834 10 Size: 54 1425 343 1144 858 3668 Fragments arranged by size: 3668 1425 1144 858 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2766 3002 3665 4077 4190 5450 5809 Size: 648 1600 236 663 412 113 1260 359 Cuts at: 5809 5968 518 Size: 159 2042 Fragments arranged by size: 2042 1600 1260 663 648 412 359 236 159 113 MspA1I CmG'CkG Cuts at: 1606 2383 3148 3823 4908 5585 6733 6978 1606 Size: 777 765 675 1085 677 1148 245 2120 Fragments arranged by size: 2120 1148 1085 777 765 677 675 245 NarI GG'CG_CC Cuts at: 2458 2458 Size: 7492 NcoI C'CATG_G Cuts at: 513 1065 2004 2024 4144 513 Size: 552 939 20 2120 3861 Fragments arranged by size: 3861 2120 939 552 20 NdeI CA'TA_TG Cuts at: 387 4449 387 Size: 4062 3430 NheI G'CTAG_C Cuts at: 1052 4571 1052 Size: 3519 3973 NotI GC'GGCC_GC Cuts at: 4939 4939 Size: 7492 NspI r_CATG'y Cuts at: 1132 1229 3001 3145 4850 6395 1132 Size: 97 1772 144 1705 1545 2229 Fragments arranged by size: 2229 1772 1705 1545 144 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7492 PciI A'CATG_T Cuts at: 6391 6391 Size: 7492 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7492 PfoI T'CCnGG_A Cuts at: 3396 3396 Size: 7492 PmeI GTTT'AAAC Cuts at: 4796 4796 Size: 7492 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5561 5593 7074 7106 5561 Size: 32 1481 32 5947 Fragments arranged by size: 5947 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2048 3151 2048 Size: 1103 6389 PshAI GACnn'nnGTC Cuts at: 1613 2489 1613 Size: 876 6616 PsiI TTA'TAA Cuts at: 5062 5062 Size: 7492 PstI C_TGCA'G Cuts at: 830 2239 2265 3159 3504 4844 830 Size: 1409 26 894 345 1340 3478 Fragments arranged by size: 3478 1409 1340 894 345 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7492 PvuII CAG'CTG Cuts at: 2383 3823 2383 Size: 1440 6052 SalI G'TCGA_C Cuts at: 1936 4834 1936 Size: 2898 4594 SanDI GG'GwC_CC Cuts at: 2048 2048 Size: 7492 SbfI CC_TGCA'GG Cuts at: 2239 4844 2239 Size: 2605 4887 ScaI AGT'ACT Cuts at: 5740 7452 5740 Size: 1712 5780 SexAI A'CCwGG_T Cuts at: 3048 3093 3048 Size: 45 7447 SfcI C'TryA_G Cuts at: 826 1045 2235 2261 3155 3500 4840 5975 6656 Size: 219 1190 26 894 345 1340 1135 681 Cuts at: 6656 6847 826 Size: 191 1471 Fragments arranged by size: 1471 1340 1190 1135 894 681 345 219 191 26 SfiI GGCCn_nnn'nGGCC Cuts at: 3840 3840 Size: 7492 SfoI GGC'GCC Cuts at: 2459 2459 Size: 7492 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7492 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7492 SmaI CCC'GGG Cuts at: 1872 4819 1872 Size: 2947 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3031 5596 6497 6759 820 Size: 197 926 1088 2565 901 262 1553 Fragments arranged by size: 2565 1553 1088 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5283 493 Size: 4790 2702 SpeI A'CTAG_T Cuts at: 152 2521 152 Size: 2369 5123 SphI G_CATG'C Cuts at: 1132 4850 1132 Size: 3718 3774 SspI AAT'ATT Cuts at: 5 52 3226 5416 5 Size: 47 3174 2190 2081 Fragments arranged by size: 3174 2190 2081 47 StuI AGG'CCT Cuts at: 1517 2691 4693 1517 Size: 1174 2002 4316 Fragments arranged by size: 4316 2002 1174 StyI C'CwwG_G Cuts at: 513 1065 2004 2024 2686 3081 4144 4931 513 Size: 552 939 20 662 395 1063 787 3074 Fragments arranged by size: 3074 1063 939 787 662 552 395 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2705 3655 5519 5536 5689 5874 6319 Size: 1255 1125 950 1864 17 153 185 445 Cuts at: 6319 325 Size: 1498 Fragments arranged by size: 1864 1498 1255 1125 950 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5738 7450 96 Size: 274 80 33 51 1443 3761 1712 138 Fragments arranged by size: 3761 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7452 7484 7452 Size: 32 7460 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7492 XbaI T'CTAG_A Cuts at: 4828 4828 Size: 7492 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2425 2425 Size: 7492 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7492 XmaI C'CCGG_G Cuts at: 1870 4817 1870 Size: 2947 4545 XmnI GAAnn'nnTTC Cuts at: 2156 2732 4623 5621 2156 Size: 576 1891 998 4027 Fragments arranged by size: 4027 1891 998 576 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3331 5300 276 Size: 53 83 186 936 1797 1969 2468 Fragments arranged by size: 2468 1969 1797 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SrfI SwaI