Restriction map for
pFN21AA0471
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglI BlnI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CfoI ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI StuI SwaI TfiI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 953 AAATTC [GA]^AATT[CT]
AflII 880 CTTAAG C^TTAAG
AgeI 1080 ACCGGT A^CCGGT
AlwI 495 GGATC GGATC(4/5)
AlwNI 550 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 953 AAATTC [GA]^AATT[CT]
Asp700I 178 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 843 GGACC G^G[AT]CC
BfrI 880 CTTAAG C^TTAAG
BglII 689 AGATCT A^GATCT
BlpI 34 GCTAAGC GC^T[ACGT]AGC
BpmI 715 CTGGAG CTGGAG(16/14)
Bpu1102I 34 GCTAAGC GC^T[ACGT]AGC
BsaWI 1080 ACCGGT [AT]^CCGG[AT]
BsmFI 60 GTCCC GGGAC(10/14)
BsrFI 1080 ACCGGT [GA]^CCGG[CT]
BstAPI 779 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1083 GGTCACC G^GT[ACGT]ACC
BstF5I 131 GGATG GGATG(2/0)
BstPI 1083 GGTCACC G^GT[ACGT]ACC
BstYI 689 AGATCT [GA]^GATC[CT]
CelII 34 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1080 ACCGGT [GA]^CCGG[CT]
Cfr13I 843 GGACC G^G[ACGT]CC
DraI 210 TTTAAA TTT^AAA
DsaI 1052 CCACGG C^C[GA][CT]GG
EaeI 1049 CGGCCA [CT]^GGCC[GA]
EcoNI 183 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1083 GGTCACC G^GT[ACGT]ACC
EcoT14I 1097 CCAAGG C^C[AT][AT]GG
FokI 131 GGATG GGATG(9/13)
HapII 1081 CCGG C^CGG
HpaII 1081 CCGG C^CGG
MflI 689 AGATCT [GA]^GATC[CT]
MslI 784 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1081 CCGG C^CGG
PinAI 1080 ACCGGT A^CCGGT
SacI 532 GAGCTC GAGCT^C
Sau96I 843 GGACC G^G[ACGT]CC
SfaNI 138 GCATC GCATC(5/9)
SmlI 880 CTTAAG C^T[CT][GA]AG
SpeI 459 ACTAGT A^CTAGT
SphI 775 GCATGC GCATG^C
SspI 289 AATATT AAT^ATT
StyI 1097 CCAAGG C^C[AT][AT]GG
TaqI 111 TCGA T^CGA
TthHB8I 111 TCGA T^CGA
XhoII 689 AGATCT [GA]^GATC[CT]
XmnI 178 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 298 GTAC GT^AC
707 GTAC
AspHI 532 GAGCTC G[AT]GC[AT]^C
1104 GAGCAC
BanI 12 GGTGCC G^G[CT][GA]CC
1057 GGCACC
BanII 532 GAGCTC G[GA]GC[CT]^C
980 GAGCCC
BsiHKAI 532 GAGCTC G[AT]GC[AT]^C
1104 GAGCAC
BsmAI 562 GAGAC GTCTC(1/5)
595 GAGAC
BsrDI 672 CATTGC GCAATG(2/0)
1067 CATTGC
BsrI 906 ACTGG ACTGG(1/-1)
912 ACTGG
DpnII 496 GATC ^GATC
690 GATC
HinfI 351 GAGTC G^A[ACGT]TC
874 GACTC
HphI 93 GGTGA GGTGA(8/7)
1085 TCACC
MboI 496 GATC ^GATC
690 GATC
NdeII 496 GATC ^GATC
690 GATC
NspI 68 ACATGC [GA]CATG^[CT]
775 GCATGC
PleI 874 GACTC GAGTC(4/5)
351 GAGTC
RsaI 298 GTAC GT^AC
707 GTAC
Sau3AI 496 GATC ^GATC
690 GATC
Tsp45I 791 GTGAC ^GT[GC]AC
1084 GTCAC
TspRI 784 CACTG CA[GC]TG[ACGT][ACGT]^
789 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI BbsI BbvI BfaI BmyI
BpuAI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I DdeI
EarI EcoRII Fnu4HI HaeIII ItaI
Ksp632I MaeI MaeIII MboII MnlI
MseI MspA1I MvaI MwoI NlaIII
NlaIV PstI PvuII ScrFI SfcI
Tru9I TseI Tsp509I XcmI