(Circular) (Six-base) MAPSORT of: pFN21AA0471.seq Check: 1943 from: 1 to: 5815 pFN21AA0471 5815 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 2592 2608 3878 5262 5530 1632 Size: 707 253 16 1270 1384 268 1917 Fragments arranged by size: 1917 1384 1270 707 268 253 16 AflII C'TTAA_G Cuts at: 820 1017 2885 820 Size: 197 1868 3750 Fragments arranged by size: 3750 1868 197 AflIII A'CryG_T Cuts at: 1203 4714 5691 1203 Size: 3511 977 1327 Fragments arranged by size: 3511 1327 977 AgeI A'CCGG_T Cuts at: 3085 4646 3085 Size: 1561 4254 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 2793 1166 Size: 1627 4188 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 2560 3558 5130 2560 Size: 998 1572 3245 Fragments arranged by size: 3245 1572 998 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 2958 3124 3350 3502 5611 1493 Size: 1465 166 226 152 2109 1697 Fragments arranged by size: 2109 1697 1465 226 166 152 AseI AT'TA_AT Cuts at: 160 1032 4369 160 Size: 872 3337 1606 Fragments arranged by size: 3337 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 3140 1870 Size: 73 1197 4545 Fragments arranged by size: 4545 1197 73 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 3062 3136 4591 618 Size: 325 217 627 230 1045 74 1455 1842 Fragments arranged by size: 1842 1455 1045 627 325 230 217 74 BanII G_rGCy'C Cuts at: 1391 2541 2989 3134 1391 Size: 1150 448 145 4072 Fragments arranged by size: 4072 1150 448 145 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2190 2278 2379 2517 2614 2669 928 Size: 971 291 88 101 138 97 55 4074 Fragments arranged by size: 4074 971 291 138 101 97 88 55 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4006 4040 1903 Size: 34 2069 34 3678 Fragments arranged by size: 3678 2069 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4426 136 Size: 107 122 71 631 605 2754 1525 Fragments arranged by size: 2754 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2694 5810 2694 Size: 3116 2699 BlpI GC'TnA_GC Cuts at: 2040 3232 2040 Size: 1192 4623 Bme1580I G_kGCm'C Cuts at: 1790 2022 3065 3876 5032 1790 Size: 232 1043 811 1156 2573 Fragments arranged by size: 2573 1156 1043 811 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5815 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3546 4504 442 Size: 416 53 83 399 24 2129 958 1753 Fragments arranged by size: 2129 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5815 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2896 2896 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2741 4475 1239 Size: 1502 1734 2579 Fragments arranged by size: 2579 1734 1502 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3940 4805 5103 3940 Size: 865 298 4652 Fragments arranged by size: 4652 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3606 493 Size: 711 2402 2702 Fragments arranged by size: 2702 2402 711 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3622 4004 4639 275 Size: 53 83 186 936 2089 382 635 1451 Fragments arranged by size: 2089 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3085 4248 4646 4920 5067 5478 3085 Size: 1163 398 274 147 411 3422 Fragments arranged by size: 3422 1163 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2936 2966 1178 Size: 30 165 30 1533 30 4027 Fragments arranged by size: 4027 1533 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2101 2591 2762 2997 1186 Size: 915 490 171 235 4004 Fragments arranged by size: 4004 915 490 235 171 BseYI C'CCAG_C Cuts at: 2026 2032 2607 3066 3093 5018 5514 2026 Size: 6 575 459 27 1925 496 2327 Fragments arranged by size: 2327 1925 575 496 459 27 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5242 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3265 4026 5054 1540 Size: 275 186 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2541 3113 3876 3961 5032 2541 Size: 572 763 85 1071 3324 Fragments arranged by size: 3324 1071 763 572 85 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2541 2989 3065 3113 3134 3876 Size: 399 232 519 448 76 48 21 742 Cuts at: 3876 3961 5032 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 519 448 399 232 85 76 48 21 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 3704 5434 3704 Size: 1730 4085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3170 844 Size: 2326 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3702 1394 Size: 429 1879 3507 Fragments arranged by size: 3507 1879 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2676 3071 4310 4484 65 Size: 1183 1428 395 1239 174 1396 Fragments arranged by size: 1428 1396 1239 1183 395 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3085 4459 4646 1700 Size: 110 1275 1374 187 2869 Fragments arranged by size: 2869 1374 1275 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5815 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5815 BssSI C'ACGA_G Cuts at: 3875 4887 3875 Size: 1012 4803 BstAPI GCAn_nnn'nTGC Cuts at: 794 2790 794 Size: 1996 3819 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 3088 1184 Size: 1904 3911 BstYI r'GATC_y Cuts at: 1972 2694 3145 3512 3897 3914 5366 5452 5464 Size: 722 451 367 385 17 1452 86 12 Cuts at: 5464 5810 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 722 451 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3057 513 Size: 552 939 1053 3271 Fragments arranged by size: 3271 1053 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5076 Fragments arranged by size: 5076 683 56 BtsI GCAGTG_nn' Cuts at: 2788 2800 3331 4125 4145 2788 Size: 12 531 794 20 4458 Fragments arranged by size: 4458 794 531 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 2217 3119 2217 Size: 902 4913 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4822 809 Size: 507 3506 1802 Fragments arranged by size: 3506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3054 3262 4151 Size: 54 1425 50 275 18 1224 208 889 Cuts at: 4151 5780 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1224 889 275 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3262 1537 Size: 275 1450 4090 Fragments arranged by size: 4090 1450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2568 2576 2592 3745 1353 Size: 1215 8 16 1153 3423 Fragments arranged by size: 3423 1215 1153 16 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4402 4776 4922 387 Size: 1064 138 2813 374 146 1280 Fragments arranged by size: 2813 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2539 2539 Size: 5815 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2592 2608 2741 3878 4475 5262 Size: 393 707 253 16 133 1137 597 787 Cuts at: 5262 5530 1239 Size: 268 1524 Fragments arranged by size: 1524 1137 787 707 597 393 268 253 133 16 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2192 Size: 5815 EcoRI G'AATT_C Cuts at: 3124 3502 3124 Size: 378 5437 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2558 2558 Size: 5815 FspI TGC'GCA Cuts at: 4321 4321 Size: 5815 HaeII r_GCGC'y Cuts at: 1678 4962 1678 Size: 3284 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2128 2160 4456 4488 4530 Size: 32 135 32 556 32 2296 32 42 Cuts at: 4530 4562 1373 Size: 32 2626 Fragments arranged by size: 2626 2296 556 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3405 669 Size: 988 281 1221 246 3079 Fragments arranged by size: 3079 1221 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 3414 3414 Size: 5815 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2412 4929 5113 1305 Size: 1107 2517 184 2007 Fragments arranged by size: 2517 2007 1107 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3993 Fragments arranged by size: 3993 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2793 3773 4132 4291 518 Size: 648 1627 980 359 159 2042 Fragments arranged by size: 2042 1627 980 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2036 2362 2896 3231 3908 5056 5301 1606 Size: 430 326 534 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 534 430 335 326 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4324 Fragments arranged by size: 4324 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5815 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 2077 2784 3173 4718 1132 Size: 97 848 707 389 1545 2229 Fragments arranged by size: 2229 1545 848 707 389 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5815 PciI A'CATG_T Cuts at: 4714 4714 Size: 5815 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5815 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PstI C_TGCA'G Cuts at: 830 2196 2795 2937 3167 830 Size: 1366 599 142 230 3478 Fragments arranged by size: 3478 1366 599 230 142 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 PvuII CAG'CTG Cuts at: 2036 2362 2896 2036 Size: 326 534 4955 Fragments arranged by size: 4955 534 326 SacI G_AGCT'C Cuts at: 2541 2541 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SfcI C'TryA_G Cuts at: 826 1045 2192 2667 2791 2933 3163 4298 4979 Size: 219 1147 475 124 142 230 1135 681 Cuts at: 4979 5170 826 Size: 191 1471 Fragments arranged by size: 1471 1147 1135 681 475 230 219 191 142 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2885 3919 4820 5082 820 Size: 197 926 942 1034 901 262 1553 Fragments arranged by size: 1553 1034 942 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 2464 152 Size: 2312 3503 SphI G_CATG'C Cuts at: 1132 2784 3173 1132 Size: 1652 389 3774 Fragments arranged by size: 3774 1652 389 SspI AAT'ATT Cuts at: 5 52 2296 3739 5 Size: 47 2244 1443 2081 Fragments arranged by size: 2244 2081 1443 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5815 StyI C'CwwG_G Cuts at: 513 1065 2004 3102 3254 513 Size: 552 939 1098 152 3074 Fragments arranged by size: 3074 1098 939 552 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3080 3107 3115 3842 3859 4012 4197 Size: 1255 1500 27 8 727 17 153 185 Cuts at: 4197 4642 325 Size: 445 1498 Fragments arranged by size: 1500 1498 1255 727 445 185 153 27 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4061 5773 96 Size: 274 80 33 51 1443 2084 1712 138 Fragments arranged by size: 2084 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2936 2968 5775 5807 2936 Size: 32 2807 32 2944 Fragments arranged by size: 2944 2807 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5815 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 2557 2935 2428 Size: 129 378 5308 Fragments arranged by size: 5308 378 129 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5815 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 2187 3944 2187 Size: 1757 4058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI