Restriction map for
pFN21AA0471
(Linear) (Six-base) MAPSORT of: KIAA0471.seq Check: 3885 from: 1 to: 1110
KIAA0471.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 587 603 1110
Size: 334 253 16 507
Fragments arranged by size:
507 334 253 16
AflII C'TTAA_G
Cuts at: 0 880 1110
Size: 880 230
AgeI A'CCGG_T
Cuts at: 0 1080 1110
Size: 1080 30
AleI CACnn'nnGTG
Cuts at: 0 788 1110
Size: 788 322
AlwNI CAG_nnn'CTG
Cuts at: 0 555 1110
Size: 555 555
ApoI r'AATT_y
Cuts at: 0 953 1110
Size: 953 157
BanI G'GyrC_C
Cuts at: 0 12 1057 1110
Size: 12 1045 53
Fragments arranged by size:
1045 53 12
BanII G_rGCy'C
Cuts at: 0 536 984 1110
Size: 536 448 126
Fragments arranged by size:
536 448 126
BbsI GAAGACnn'nnnn_
Cuts at: 0 185 273 374 512 609 664 1110
Size: 185 88 101 138 97 55 446
Fragments arranged by size:
446 185 138 101 97 88 55
BglII A'GATC_T
Cuts at: 0 689 1110
Size: 689 421
BlpI GC'TnA_GC
Cuts at: 0 35 1110
Size: 35 1075
Bme1580I G_kGCm'C
Cuts at: 0 17 1060 1110
Size: 17 1043 50
Fragments arranged by size:
1043 50 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 891 1110
Size: 891 219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1110
Size: 736 374
BsaWI w'CCGG_w
Cuts at: 0 1080 1110
Size: 1080 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 931 961 1110
Size: 931 30 149
Fragments arranged by size:
931 149 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 586 757 992 1110
Size: 96 490 171 235 118
Fragments arranged by size:
490 235 171 118 96
BseYI C'CCAG_C
Cuts at: 0 21 27 602 1061 1088 1110
Size: 21 6 575 459 27 22
Fragments arranged by size:
575 459 27 22 21 6
BsiHKAI G_wGCw'C
Cuts at: 0 536 1108 1110
Size: 536 572 2
Fragments arranged by size:
572 536 2
Bsp1286I G_dGCh'C
Cuts at: 0 17 536 984 1060 1108 1110
Size: 17 519 448 76 48 2
Fragments arranged by size:
519 448 76 48 17 2
BsrDI GCAATG_nn'
Cuts at: 0 671 1066 1110
Size: 671 395 44
Fragments arranged by size:
671 395 44
BsrFI r'CCGG_y
Cuts at: 0 1080 1110
Size: 1080 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 785 1110
Size: 785 325
BstEII G'GTnAC_C
Cuts at: 0 1083 1110
Size: 1083 27
BstYI r'GATC_y
Cuts at: 0 689 1110
Size: 689 421
BtgI C'CryG_G
Cuts at: 0 1052 1110
Size: 1052 58
BtsI GCAGTG_nn'
Cuts at: 0 783 795 1110
Size: 783 12 315
Fragments arranged by size:
783 315 12
DraI TTT'AAA
Cuts at: 0 212 1110
Size: 212 898
EaeI y'GGCC_r
Cuts at: 0 1049 1110
Size: 1049 61
EarI CTCTTCn'nnn_
Cuts at: 0 563 571 587 1110
Size: 563 8 16 523
Fragments arranged by size:
563 523 16 8
EcoICRI GAG'CTC
Cuts at: 0 534 1110
Size: 534 576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 587 603 736 1110
Size: 334 253 16 133 374
Fragments arranged by size:
374 334 253 133 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 1110
Size: 187 923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 553 1110
Size: 553 557
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 1110
Size: 123 32 955
Fragments arranged by size:
955 123 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 1110
Size: 407 703
MslI CAynn'nnrTG
Cuts at: 0 788 1110
Size: 788 322
MspA1I CmG'CkG
Cuts at: 0 31 357 891 1110
Size: 31 326 534 219
Fragments arranged by size:
534 326 219 31
NspI r_CATG'y
Cuts at: 0 72 779 1110
Size: 72 707 331
Fragments arranged by size:
707 331 72
PstI C_TGCA'G
Cuts at: 0 191 790 932 1110
Size: 191 599 142 178
Fragments arranged by size:
599 191 178 142
PvuII CAG'CTG
Cuts at: 0 31 357 891 1110
Size: 31 326 534 219
Fragments arranged by size:
534 326 219 31
SacI G_AGCT'C
Cuts at: 0 536 1110
Size: 536 574
SfcI C'TryA_G
Cuts at: 0 187 662 786 928 1110
Size: 187 475 124 142 182
Fragments arranged by size:
475 187 182 142 124
SmlI C'TyrA_G
Cuts at: 0 880 1110
Size: 880 230
SpeI A'CTAG_T
Cuts at: 0 459 1110
Size: 459 651
SphI G_CATG'C
Cuts at: 0 779 1110
Size: 779 331
SspI AAT'ATT
Cuts at: 0 291 1110
Size: 291 819
StyI C'CwwG_G
Cuts at: 0 1097 1110
Size: 1097 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1075 1102 1110
Size: 1075 27 8
Fragments arranged by size:
1075 27 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 931 963 1110
Size: 931 32 147
Fragments arranged by size:
931 147 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 552 930 1110
Size: 423 129 378 180
Fragments arranged by size:
423 378 180 129
XmnI GAAnn'nnTTC
Cuts at: 0 182 1110
Size: 182 928
Enzymes that do cut:
AcuI AflII AgeI AleI AlwNI ApoI BanI BanII
BbsI BglII BlpI Bme1580I BplI BpmI BsaWI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BsrDI BsrFI BstAPI BstEII
BstYI BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoNI FalI Hin4I MmeI MslI MspA1I NspI PstI
PvuII SacI SfcI SmlI SpeI SphI SspI StyI
TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BmgBI BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
StuI SwaI TatI Tth111I XbaI XhoI XmaI ZraI