Restriction map for pFN21AA0455
 (Linear) (Six-base) MAPSORT of: KIAA0455.seq  Check: 6993  from: 1  to: 2289



KIAA0455.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1221    1808    1937    2289 
   Size:       1221     587     129     352

  Fragments arranged by size: 

                1221     587     352     129
 
AhdI GACnn_n'nnGTC

Cuts at:      0     523    2289 
   Size:        523    1766
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1264    2289 
   Size:       1264    1025
 
AlwNI CAG_nnn'CTG

Cuts at:      0     706    2289 
   Size:        706    1583
 
ApaLI G'TGCA_C

Cuts at:      0      97     289    1600    2289 
   Size:         97     192    1311     689

  Fragments arranged by size: 

                1311     689     192      97
 
ApoI r'AATT_y

Cuts at:      0     425     672     984    2289 
   Size:        425     247     312    1305

  Fragments arranged by size: 

                1305     425     312     247
 
AseI AT'TA_AT

Cuts at:      0     482    2289 
   Size:        482    1807
 
AvaI C'yCGr_G

Cuts at:      0     109     127    1658    2289 
   Size:        109      18    1531     631

  Fragments arranged by size: 

                1531     631     109      18
 
BanI G'GyrC_C

Cuts at:      0      49    2289 
   Size:         49    2240
 
BanII G_rGCy'C

Cuts at:      0     476    1210    1850    1988    2289 
   Size:        476     734     640     138     301

  Fragments arranged by size: 

                 734     640     476     301     138
 
BbeI G_GCGC'C

Cuts at:      0      53    2289 
   Size:         53    2236
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     270     840    1283    2289 
   Size:        270     570     443    1006

  Fragments arranged by size: 

                1006     570     443     270
 
BbvCI CC'TCA_GC

Cuts at:      0      85    2289 
   Size:         85    2204
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     128     162    2289 
   Size:        128      34    2127

  Fragments arranged by size: 

                2127     128      34
 
BclI T'GATC_A

Cuts at:      0     176    2289 
   Size:        176    2113
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1554    2289 
   Size:       1554     735
 
BlpI GC'TnA_GC

Cuts at:      0     376    2289 
   Size:        376    1913
 
Bme1580I G_kGCm'C

Cuts at:      0     101     293    1604    2289 
   Size:        101     192    1311     685

  Fragments arranged by size: 

                1311     685     192     101
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     907     989    1341    1844    2113    2289 
   Size:        907      82     352     503     269     176

  Fragments arranged by size: 

                 907     503     352     269     176      82
 
BmtI G_CTAG'C

Cuts at:      0    1151    2289 
   Size:       1151    1138
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1045    2289 
   Size:       1045    1244
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     509    1571    2066    2289 
   Size:         88     421    1062     495     223

  Fragments arranged by size: 

                1062     495     421     223      88
 
Bpu10I CC'TnA_GC

Cuts at:      0      85     699    1400    2289 
   Size:         85     614     701     889

  Fragments arranged by size: 

                 889     701     614      85
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1040    1469    1806    2289 
   Size:       1040     429     337     483

  Fragments arranged by size: 

                1040     483     429     337
 
BsaHI Gr'CG_yC

Cuts at:      0      50    1025    2289 
   Size:         50     975    1264

  Fragments arranged by size: 

                1264     975      50
 
BsaWI w'CCGG_w

Cuts at:      0     314    2289 
   Size:        314    1975
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     959    1330    1627    2289 
   Size:         85     874     371     297     662

  Fragments arranged by size: 

                 874     662     371     297      85
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1791    2289 
   Size:       1791     498
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     293    1604    1988    2289 
   Size:        101     192    1311     384     301

  Fragments arranged by size: 

                1311     384     301     192     101
 
BsmI GAATG_Cn'

Cuts at:      0    1301    2289 
   Size:       1301     988
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1016    2140    2289 
   Size:       1016    1124     149

  Fragments arranged by size: 

                1124    1016     149
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     293     476    1210    1604    1850    1988    2289 
   Size:        101     192     183     734     394     246     138     301

  Fragments arranged by size: 

                 734     394     301     246     192     183     138     101
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     405    2289 
   Size:        405    1884
 
BsrBI CCG'CTC

Cuts at:      0    1324    2289 
   Size:       1324     965
 
BsrDI GCAATG_nn'

Cuts at:      0    2082    2289 
   Size:       2082     207
 
BssHII G'CGCG_C

Cuts at:      0       6    2289 
   Size:          6    2283
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     763    2289 
   Size:        763    1526
 
BstEII G'GTnAC_C

Cuts at:      0    1230    2289 
   Size:       1230    1059
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1489    2289 
   Size:       1489     800
 
BstYI r'GATC_y

Cuts at:      0     703    1056    1423    1904    2289 
   Size:        703     353     367     481     385

  Fragments arranged by size: 

                 703     481     385     367     353
 
Bsu36I CC'TnA_GG

Cuts at:      0    1030    1610    2289 
   Size:       1030     580     679

  Fragments arranged by size: 

                1030     679     580
 
BtgI C'CryG_G

Cuts at:      0      27    1201    1211    1482    2091    2289 
   Size:         27    1174      10     271     609     198

  Fragments arranged by size: 

                1174     609     271     198      27      10
 
BtsI GCAGTG_nn'

Cuts at:      0    1101    1935    2289 
   Size:       1101     834     354

  Fragments arranged by size: 

                1101     834     354
 
DraIII CAC_nnn'GTG

Cuts at:      0    1349    2289 
   Size:       1349     940
 
EaeI y'GGCC_r

Cuts at:      0      24    2289 
   Size:         24    2265
 
EcoICRI GAG'CTC

Cuts at:      0    1986    2289 
   Size:       1986     303
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     509    1221    1571    1808    1937    2066    2289 
   Size:         88     421     712     350     237     129     129     223

  Fragments arranged by size: 

                 712     421     350     237     223     129     129      88
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1586    2289 
   Size:       1586     703
 
EcoRI G'AATT_C

Cuts at:      0     425    2289 
   Size:        425    1864
 
EcoRV GAT'ATC

Cuts at:      0     582     604    2289 
   Size:        582      22    1685

  Fragments arranged by size: 

                1685     582      22
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1398    2289 
   Size:       1398     891
 
HaeII r_GCGC'y

Cuts at:      0      53      60    1523    2289 
   Size:         53       7    1463     766

  Fragments arranged by size: 

                1463     766      53       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     509     541     682     695     714     727    1135    1167 
   Size:        509      32     141      13      19      13     408      32

Cuts at:   1167    2137    2169    2289 
   Size:        970      32     120

  Fragments arranged by size: 

                 970     509     408     141     120      32      32      32
                  19      13      13
 
HindIII A'AGCT_T

Cuts at:      0     826    2289 
   Size:        826    1463
 
KasI G'GCGC_C

Cuts at:      0      49    2289 
   Size:         49    2240
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     444    2289 
   Size:        444    1845
 
MslI CAynn'nnrTG

Cuts at:      0    1142    1432    1741    2289 
   Size:       1142     290     309     548

  Fragments arranged by size: 

                1142     548     309     290
 
MspA1I CmG'CkG

Cuts at:      0      20      29      47      89     591     778    2289 
   Size:         20       9      18      42     502     187    1511

  Fragments arranged by size: 

                1511     502     187      42      20      18       9
 
NarI GG'CG_CC

Cuts at:      0      50    2289 
   Size:         50    2239
 
NcoI C'CATG_G

Cuts at:      0    1211    1482    2289 
   Size:       1211     271     807

  Fragments arranged by size: 

                1211     807     271
 
NheI G'CTAG_C

Cuts at:      0    1147    2289 
   Size:       1147    1142
 
NspI r_CATG'y

Cuts at:      0     330     757    1548    2289 
   Size:        330     427     791     741

  Fragments arranged by size: 

                 791     741     427     330
 
PfoI T'CCnGG_A

Cuts at:      0    1936    2289 
   Size:       1936     353
 
PpuMI rG'GwC_Cy

Cuts at:      0    1586    2289 
   Size:       1586     703
 
PshAI GACnn'nnGTC

Cuts at:      0     317    2289 
   Size:        317    1972
 
PsiI TTA'TAA

Cuts at:      0    2282    2289 
   Size:       2282       7
 
PvuII CAG'CTG

Cuts at:      0      89     591     778    2289 
   Size:         89     502     187    1511

  Fragments arranged by size: 

                1511     502     187      89
 
SacI G_AGCT'C

Cuts at:      0    1988    2289 
   Size:       1988     301
 
SacII CC_GC'GG

Cuts at:      0      30    2289 
   Size:         30    2259
 
SfcI C'TryA_G

Cuts at:      0     563    1271    2289 
   Size:        563     708    1018

  Fragments arranged by size: 

                1018     708     563
 
SfoI GGC'GCC

Cuts at:      0      51    2289 
   Size:         51    2238
 
SmaI CCC'GGG

Cuts at:      0     111     129    2289 
   Size:        111      18    2160

  Fragments arranged by size: 

                2160     111      18
 
SphI G_CATG'C

Cuts at:      0     330    1548    2289 
   Size:        330    1218     741

  Fragments arranged by size: 

                1218     741     330
 
StyI C'CwwG_G

Cuts at:      0    1211    1482    2289 
   Size:       1211     271     807

  Fragments arranged by size: 

                1211     807     271
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     926    1360    1434    1892    2289 
   Size:        926     434      74     458     397

  Fragments arranged by size: 

                 926     458     434     397      74
 
TatI w'GTAC_w

Cuts at:      0     794     959    2289 
   Size:        794     165    1330

  Fragments arranged by size: 

                1330     794     165
 
Tth111I GACn'n_nGTC

Cuts at:      0     187    1282    2151    2289 
   Size:        187    1095     869     138

  Fragments arranged by size: 

                1095     869     187     138
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1486    1849    2289 
   Size:       1486     363     440

  Fragments arranged by size: 

                1486     440     363
 
XmaI C'CCGG_G

Cuts at:      0     109     127    2289 
   Size:        109      18    2162

  Fragments arranged by size: 

                2162     109      18
 
XmnI GAAnn'nnTTC

Cuts at:      0     425    2289 
   Size:        425    1864
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI    AlwNI    ApaLI     ApoI     AseI     AvaI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaHI    BsaWI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI   BssHII   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI   DraIII     EaeI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    HaeII    Hin4I  HindIII     KasI     MmeI
     MslI   MspA1I     NarI     NcoI     NheI     NspI     PfoI    PpuMI
    PshAI     PsiI    PvuII     SacI    SacII     SfcI     SfoI     SmaI
     SphI     StyI    TaqII     TatI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AloI     ApaI     AscI    AsiSI    AvrII     BaeI
    BamHI    BciVI    BfrBI    BglII    BmgBI    BpuEI    BsaAI    BsaBI
    BsaXI    BseYI    BsiEI    BsiWI    BspEI    BspHI    BsrFI    BsrGI
    BssSI    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI
     EagI     EarI     EciI    EcoNI     FseI     FspI    FspAI   HincII
     HpaI     KpnI     MfeI     MluI     MscI     NaeI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     TstI     XbaI     XhoI     ZraI
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