(Circular) (Six-base) MAPSORT of: pFN21AA0447.seq Check: 4168 from: 1 to: 5920 pFN21AA0447 5920 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2844 3730 278 Size: 53 83 186 936 1308 886 2468 Fragments arranged by size: 2468 1308 936 886 186 83 53 AccI GT'mk_AC Cuts at: 1937 3263 1937 Size: 1326 4594 Acc65I G'GTAC_C Cuts at: 3241 3241 Size: 5920 AclI AA'CG_TT Cuts at: 1563 4047 4420 1563 Size: 2484 373 3063 Fragments arranged by size: 3063 2484 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2242 2857 2915 3983 5367 5635 1632 Size: 610 615 58 1068 1384 268 1917 Fragments arranged by size: 1917 1384 1068 615 610 268 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5723 AflIII A'CryG_T Cuts at: 1203 4819 5796 1203 Size: 3616 977 1327 Fragments arranged by size: 3616 1327 977 AgeI A'CCGG_T Cuts at: 4751 4751 Size: 5920 AhdI GACnn_n'nnGTC Cuts at: 4649 4649 Size: 5920 AleI CACnn'nnGTG Cuts at: 1166 2112 1166 Size: 946 4974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5920 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2765 2797 2765 Size: 32 5888 AlwNI CAG_nnn'CTG Cuts at: 2265 2341 3145 3158 3663 5235 2265 Size: 76 804 13 505 1572 2950 Fragments arranged by size: 2950 1572 804 505 76 13 ApaI G_GGCC'C Cuts at: 3169 3169 Size: 5920 ApaLI G'TGCA_C Cuts at: 2641 3977 5133 2641 Size: 1336 1156 3428 Fragments arranged by size: 3428 1336 1156 ApoI r'AATT_y Cuts at: 1493 3229 3455 3607 5716 1493 Size: 1736 226 152 2109 1697 Fragments arranged by size: 2109 1736 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4474 160 Size: 872 3442 1606 Fragments arranged by size: 3442 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 AvaI C'yCGr_G Cuts at: 1870 1943 2823 3245 1870 Size: 73 880 422 4545 Fragments arranged by size: 4545 880 422 73 BamHI G'GATC_C Cuts at: 3250 3617 3250 Size: 367 5553 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3241 4696 618 Size: 325 217 627 1454 1455 1842 Fragments arranged by size: 1842 1455 1454 627 325 217 BanII G_rGCy'C Cuts at: 1391 2096 2222 2316 2537 3169 3239 1391 Size: 705 126 94 221 632 70 4072 Fragments arranged by size: 4072 705 632 221 126 94 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4949 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4111 4145 1903 Size: 34 2174 34 3678 Fragments arranged by size: 3678 2174 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3812 5022 3812 Size: 1210 4710 BclI T'GATC_A Cuts at: 1548 2556 1548 Size: 1008 4912 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4531 136 Size: 107 122 71 631 605 2859 1525 Fragments arranged by size: 2859 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5915 5915 Size: 5920 BlpI GC'TnA_GC Cuts at: 2741 3023 3337 2741 Size: 282 314 5324 Fragments arranged by size: 5324 314 282 Bme1580I G_kGCm'C Cuts at: 1790 2645 3169 3981 5137 1790 Size: 855 524 812 1156 2573 Fragments arranged by size: 2573 1156 855 812 524 BmgBI CAC'GTC Cuts at: 1318 2890 1318 Size: 1572 4348 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2612 2840 3651 Size: 416 53 83 399 24 1195 228 811 Cuts at: 3651 4609 442 Size: 958 1753 Fragments arranged by size: 1753 1195 958 811 416 399 228 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5920 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4580 1239 Size: 3341 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4045 4910 5208 4045 Size: 865 298 4757 Fragments arranged by size: 4757 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2177 4583 882 Size: 617 678 2406 2219 Fragments arranged by size: 2406 2219 678 617 BsaAI yAC'GTr Cuts at: 493 1204 3711 493 Size: 711 2507 2702 Fragments arranged by size: 2702 2507 711 BsaBI GATnn'nnATC Cuts at: 5914 5914 Size: 5920 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2841 3727 4109 4744 Size: 53 83 186 936 1308 886 382 635 Cuts at: 4744 275 Size: 1451 Fragments arranged by size: 1451 1308 936 886 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4353 4751 5025 5172 5583 4353 Size: 398 274 147 411 4690 Fragments arranged by size: 4690 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2765 2795 1178 Size: 30 165 30 1362 30 4303 Fragments arranged by size: 4303 1362 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2872 1186 Size: 1686 4234 BseYI C'CCAG_C Cuts at: 2026 2088 2302 2315 5123 5619 2026 Size: 62 214 13 2808 496 2327 Fragments arranged by size: 2808 2327 496 214 62 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2251 2354 1163 Size: 573 515 103 4729 Fragments arranged by size: 4729 573 515 103 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2685 3066 3370 4131 5159 1540 Size: 275 186 684 381 304 761 1028 2301 Fragments arranged by size: 2301 1028 761 684 381 304 275 186 BsiHKAI G_wGCw'C Cuts at: 2222 2537 2645 3981 4066 5137 2222 Size: 315 108 1336 85 1071 3005 Fragments arranged by size: 3005 1336 1071 315 108 85 BsiWI C'GTAC_G Cuts at: 2946 2946 Size: 5920 BsmI GAATG_Cn' Cuts at: 3431 3524 3431 Size: 93 5827 BsmBI CGTCTCn'nnnn_ Cuts at: 2516 2516 Size: 5920 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2222 2316 2537 2645 3169 3239 Size: 399 306 126 94 221 108 524 70 Cuts at: 3239 3981 4066 5137 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 524 399 306 221 126 108 94 85 70 BspEI T'CCGG_A Cuts at: 5583 5583 Size: 5920 BspHI T'CATG_A Cuts at: 3809 5539 3809 Size: 1730 4190 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2064 3275 844 Size: 1220 1211 3489 Fragments arranged by size: 3489 1220 1211 BsrBI CCG'CTC Cuts at: 1394 1823 2862 3807 1394 Size: 429 1039 945 3507 Fragments arranged by size: 3507 1039 945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4415 4589 65 Size: 1183 3167 174 1396 Fragments arranged by size: 3167 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4564 4751 1700 Size: 110 2754 187 2869 Fragments arranged by size: 2869 2754 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5920 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5920 BssSI C'ACGA_G Cuts at: 3980 4992 3980 Size: 1012 4908 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5920 BstBI TT'CG_AA Cuts at: 4724 4724 Size: 5920 BstEII G'GTnAC_C Cuts at: 1184 2105 3082 1184 Size: 921 977 4022 Fragments arranged by size: 4022 977 921 BstXI CCAn_nnnn'nTGG Cuts at: 2704 2704 Size: 5920 BstYI r'GATC_y Cuts at: 1972 3250 3617 4002 4019 5471 5557 5569 5915 Size: 1278 367 385 17 1452 86 12 346 Cuts at: 5915 1972 Size: 1977 Fragments arranged by size: 1977 1452 1278 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2187 2349 513 Size: 552 1122 162 4084 Fragments arranged by size: 4084 1122 552 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5181 Fragments arranged by size: 5181 683 56 BtsI GCAGTG_nn' Cuts at: 3436 4230 4250 3436 Size: 794 20 5106 Fragments arranged by size: 5106 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5920 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5885 DraI TTT'AAA Cuts at: 3224 3224 Size: 5920 DraIII CAC_nnn'GTG Cuts at: 2247 2247 Size: 5920 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4927 809 Size: 507 3611 1802 Fragments arranged by size: 3611 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2646 2682 2805 Size: 54 1425 50 275 18 816 36 123 Cuts at: 2805 3063 3160 3367 4256 5885 8 Size: 258 97 207 889 1629 43 Fragments arranged by size: 1629 1425 889 816 275 258 207 123 97 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2682 3063 3367 1537 Size: 275 870 381 304 4090 Fragments arranged by size: 4090 870 381 304 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2052 2279 2525 2861 3850 1353 Size: 678 21 227 246 336 989 3423 Fragments arranged by size: 3423 989 678 336 246 227 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2527 4507 4881 5027 387 Size: 1064 138 938 1980 374 146 1280 Fragments arranged by size: 1980 1280 1064 938 374 146 138 EcoICRI GAG'CTC Cuts at: 2220 2535 2220 Size: 315 5605 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2242 2857 2915 3983 4580 5367 5635 Size: 393 610 615 58 1068 597 787 268 Cuts at: 5635 1239 Size: 1524 Fragments arranged by size: 1524 1068 787 615 610 597 393 268 58 EcoO109I rG'GnC_Cy Cuts at: 3094 3166 3202 3094 Size: 72 36 5812 Fragments arranged by size: 5812 72 36 EcoRI G'AATT_C Cuts at: 3229 3607 3229 Size: 378 5542 FspI TGC'GCA Cuts at: 4426 4426 Size: 5920 HaeII r_GCGC'y Cuts at: 1678 2231 2639 2796 3137 5067 1678 Size: 553 408 157 341 1930 2531 Fragments arranged by size: 2531 1930 553 408 341 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2548 2580 4561 4593 4635 Size: 32 135 32 976 32 1981 32 42 Cuts at: 4635 4667 1373 Size: 32 2626 Fragments arranged by size: 2626 1981 976 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2602 3264 3510 669 Size: 988 281 664 662 246 3079 Fragments arranged by size: 3079 988 664 662 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5920 HpaI GTT'AAC Cuts at: 3510 3510 Size: 5920 KpnI G_GTAC'C Cuts at: 3245 3245 Size: 5920 MfeI C'AATT_G Cuts at: 3519 3519 Size: 5920 MluI A'CGCG_T Cuts at: 5796 5796 Size: 5920 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5034 5218 1305 Size: 3729 184 2007 Fragments arranged by size: 3729 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2807 10 Size: 54 1425 343 975 3123 Fragments arranged by size: 3123 1425 975 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 3878 4237 4396 518 Size: 648 946 1766 359 159 2042 Fragments arranged by size: 2042 1766 946 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2088 2745 2798 3190 3336 4013 5161 5406 Size: 482 657 53 392 146 677 1148 245 Cuts at: 5406 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 657 482 392 245 146 53 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5368 NdeI CA'TA_TG Cuts at: 387 387 Size: 5920 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5920 NotI GC'GGCC_GC Cuts at: 3367 3367 Size: 5920 NspI r_CATG'y Cuts at: 1132 1229 2323 3008 3278 4823 1132 Size: 97 1094 685 270 1545 2229 Fragments arranged by size: 2229 1545 1094 685 270 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5920 PciI A'CATG_T Cuts at: 4819 4819 Size: 5920 PflMI CCAn_nnn'nTGG Cuts at: 1108 2890 1108 Size: 1782 4138 PmeI GTTT'AAAC Cuts at: 3224 3224 Size: 5920 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2765 2797 3989 4021 5502 5534 2765 Size: 32 1192 32 1481 32 3151 Fragments arranged by size: 3151 1481 1192 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3094 3202 3094 Size: 108 5812 PshAI GACnn'nnGTC Cuts at: 1613 2189 1613 Size: 576 5344 PsiI TTA'TAA Cuts at: 3490 3490 Size: 5920 PspOMI G'GGCC_C Cuts at: 3165 3165 Size: 5920 PstI C_TGCA'G Cuts at: 830 2781 3141 3154 3272 830 Size: 1951 360 13 118 3478 Fragments arranged by size: 3478 1951 360 118 13 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5920 PvuII CAG'CTG Cuts at: 2088 2088 Size: 5920 SacI G_AGCT'C Cuts at: 2222 2537 2222 Size: 315 5605 SalI G'TCGA_C Cuts at: 1936 3262 1936 Size: 1326 4594 SapI GCTCTTCn'nnn_ Cuts at: 2031 2279 2525 2861 2031 Size: 248 246 336 5090 Fragments arranged by size: 5090 336 248 246 SbfI CC_TGCA'GG Cuts at: 3272 3272 Size: 5920 ScaI AGT'ACT Cuts at: 4168 5880 4168 Size: 1712 4208 SfcI C'TryA_G Cuts at: 826 1045 2777 3137 3150 3268 4403 5084 5275 Size: 219 1732 360 13 118 1135 681 191 Cuts at: 5275 826 Size: 1471 Fragments arranged by size: 1732 1471 1135 681 360 219 191 118 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5920 SmaI CCC'GGG Cuts at: 1872 2825 3247 1872 Size: 953 422 4545 Fragments arranged by size: 4545 953 422 SmlI C'TyrA_G Cuts at: 820 1017 1943 4024 4925 5187 820 Size: 197 926 2081 901 262 1553 Fragments arranged by size: 2081 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3711 493 Size: 3218 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5920 SphI G_CATG'C Cuts at: 1132 2323 3278 1132 Size: 1191 955 3774 Fragments arranged by size: 3774 1191 955 SspI AAT'ATT Cuts at: 5 52 3844 5 Size: 47 3792 2081 Fragments arranged by size: 3792 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5920 StyI C'CwwG_G Cuts at: 513 1065 3359 513 Size: 552 2294 3074 Fragments arranged by size: 3074 2294 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2198 2437 3947 3964 4117 4302 4747 Size: 1255 618 239 1510 17 153 185 445 Cuts at: 4747 325 Size: 1498 Fragments arranged by size: 1510 1498 1255 618 445 239 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2286 2583 4166 Size: 274 80 33 51 1443 309 297 1583 Cuts at: 4166 5878 96 Size: 1712 138 Fragments arranged by size: 1712 1583 1443 309 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2412 2444 5880 5912 2412 Size: 32 3436 32 2420 Fragments arranged by size: 3436 2420 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2915 1316 Size: 1599 4321 XbaI T'CTAG_A Cuts at: 3256 3256 Size: 5920 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2701 2701 Size: 5920 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5920 XmaI C'CCGG_G Cuts at: 1870 2823 3245 1870 Size: 953 422 4545 Fragments arranged by size: 4545 953 422 XmnI GAAnn'nnTTC Cuts at: 2870 4049 2870 Size: 1179 4741 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2842 3728 276 Size: 53 83 186 936 1308 886 2468 Fragments arranged by size: 2468 1308 936 886 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI