(Circular) (Six-base) MAPSORT of: pFN21AA0443.seq Check: 8663 from: 1 to: 8890 pFN21AA0443 8890 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6700 278 Size: 53 83 186 936 5164 2468 Fragments arranged by size: 5164 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2589 3283 4663 6233 1937 Size: 652 694 1380 1570 4594 Fragments arranged by size: 4594 1570 1380 694 652 Acc65I G'GTAC_C Cuts at: 6211 6211 Size: 8890 AclI AA'CG_TT Cuts at: 1563 7017 7390 1563 Size: 5454 373 3063 Fragments arranged by size: 5454 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3102 3348 3747 4254 4533 4668 4728 4950 Size: 1470 246 399 507 279 135 60 222 Cuts at: 4950 5294 5484 5973 6192 6953 8337 8605 1632 Size: 344 190 489 219 761 1384 268 1917 Fragments arranged by size: 1917 1470 1384 761 507 489 399 344 279 268 246 222 219 190 135 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8693 AflIII A'CryG_T Cuts at: 1203 5090 7789 8766 1203 Size: 3887 2699 977 1327 Fragments arranged by size: 3887 2699 1327 977 AgeI A'CCGG_T Cuts at: 7721 7721 Size: 8890 AhdI GACnn_n'nnGTC Cuts at: 7619 7619 Size: 8890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4789 1184 Size: 3605 5285 AlwNI CAG_nnn'CTG Cuts at: 2441 3725 4007 4322 4388 4706 5414 6633 8205 Size: 1284 282 315 66 318 708 1219 1572 Cuts at: 8205 2441 Size: 3126 Fragments arranged by size: 3126 1572 1284 1219 708 318 315 282 66 ApaI G_GGCC'C Cuts at: 2829 2829 Size: 8890 ApaLI G'TGCA_C Cuts at: 6947 8103 6947 Size: 1156 7734 ApoI r'AATT_y Cuts at: 1493 2639 2951 5376 5499 5908 5966 6125 6199 Size: 1146 312 2425 123 409 58 159 74 Cuts at: 6199 6425 6577 8686 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2425 2109 1697 1146 409 312 226 159 152 123 74 58 AseI AT'TA_AT Cuts at: 160 1032 5489 7444 160 Size: 872 4457 1955 1606 Fragments arranged by size: 4457 1955 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8890 AvaI C'yCGr_G Cuts at: 1870 1943 3171 5568 5634 6215 1870 Size: 73 1228 2397 66 581 4545 Fragments arranged by size: 4545 2397 1228 581 73 66 AvrII C'CTAG_G Cuts at: 2537 2537 Size: 8890 BamHI G'GATC_C Cuts at: 5506 6220 6587 5506 Size: 714 367 7809 Fragments arranged by size: 7809 714 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 6211 7666 618 Size: 325 217 627 243 4181 1455 1842 Fragments arranged by size: 4181 1842 1455 627 325 243 217 BanII G_rGCy'C Cuts at: 1391 2293 2739 2829 3837 6209 1391 Size: 902 446 90 1008 2372 4072 Fragments arranged by size: 4072 2372 1008 902 446 90 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2916 4410 928 Size: 971 1017 1494 5408 Fragments arranged by size: 5408 1494 1017 971 BbvCI CC'TCA_GC Cuts at: 3447 3540 3822 4008 4323 3447 Size: 93 282 186 315 8014 Fragments arranged by size: 8014 315 282 186 93 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3003 3037 7081 7115 1903 Size: 34 1066 34 4044 34 3678 Fragments arranged by size: 4044 3678 1066 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4071 4792 6782 7992 4071 Size: 721 1990 1210 4969 Fragments arranged by size: 4969 1990 1210 721 BclI T'GATC_A Cuts at: 1548 3114 5317 1548 Size: 1566 2203 5121 Fragments arranged by size: 5121 2203 1566 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7501 136 Size: 107 122 71 631 605 5829 1525 Fragments arranged by size: 5829 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8885 8885 Size: 8890 BlpI GC'TnA_GC Cuts at: 2292 6307 2292 Size: 4015 4875 Bme1580I G_kGCm'C Cuts at: 1790 2035 2227 2829 6951 8107 1790 Size: 245 192 602 4122 1156 2573 Fragments arranged by size: 4122 2573 1156 602 245 192 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2019 3360 3450 Size: 416 53 83 399 24 602 1341 90 Cuts at: 3450 6621 7579 442 Size: 3171 958 1753 Fragments arranged by size: 3171 1753 1341 958 602 416 399 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3360 4415 1056 Size: 2304 1055 5531 Fragments arranged by size: 5531 2304 1055 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2353 3472 2353 Size: 1119 7771 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2205 2635 3693 3929 4230 4380 7550 1239 Size: 966 430 1058 236 301 150 3170 2579 Fragments arranged by size: 3170 2579 1058 966 430 301 236 150 Bpu10I CC'TnA_GC Cuts at: 3447 3540 3822 4008 4323 6132 3447 Size: 93 282 186 315 1809 6205 Fragments arranged by size: 6205 1809 315 282 186 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7015 7880 8178 7015 Size: 865 298 7727 Fragments arranged by size: 7727 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2504 3542 4007 4235 4457 7553 882 Size: 617 1005 1038 465 228 222 3096 2219 Fragments arranged by size: 3096 2219 1038 1005 617 465 228 222 BsaAI yAC'GTr Cuts at: 493 1204 5091 6681 493 Size: 711 3887 1590 2702 Fragments arranged by size: 3887 2702 1590 711 BsaBI GATnn'nnATC Cuts at: 8884 8884 Size: 8890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6697 7079 7714 275 Size: 53 83 186 936 5164 382 635 1451 Fragments arranged by size: 5164 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4564 7323 7721 7995 8142 8553 4564 Size: 2759 398 274 147 411 4901 Fragments arranged by size: 4901 2759 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2613 2643 2912 2942 3284 Size: 30 165 30 1210 30 269 30 342 Cuts at: 3284 3314 3666 3696 1178 Size: 30 352 30 6372 Fragments arranged by size: 6372 1210 352 342 269 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2522 3218 3839 3842 3845 4025 4112 4253 Size: 1336 696 621 3 3 180 87 141 Cuts at: 4253 4340 4625 5156 1186 Size: 87 285 531 4920 Fragments arranged by size: 4920 1336 696 621 531 285 180 141 87 87 3 3 BseYI C'CCAG_C Cuts at: 2972 4481 8093 8589 2972 Size: 1509 3612 496 3273 Fragments arranged by size: 3612 3273 1509 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2232 1163 Size: 573 496 7821 Fragments arranged by size: 7821 573 496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6340 7101 8129 1540 Size: 275 186 4339 761 1028 2301 Fragments arranged by size: 4339 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2739 6951 7036 8107 2739 Size: 4212 85 1071 3522 Fragments arranged by size: 4212 3522 1071 85 BsmI GAATG_Cn' Cuts at: 6107 6401 6494 6107 Size: 294 93 8503 Fragments arranged by size: 8503 294 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2227 2293 2739 2829 3837 6209 Size: 399 245 192 66 446 90 1008 2372 Cuts at: 6209 6951 7036 8107 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2372 2174 1071 1008 742 446 399 245 192 90 85 66 BspEI T'CCGG_A Cuts at: 4564 8553 4564 Size: 3989 4901 BspHI T'CATG_A Cuts at: 5518 5928 6779 8509 5518 Size: 410 851 1730 5899 Fragments arranged by size: 5899 1730 851 410 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2838 5073 6245 844 Size: 1994 2235 1172 3489 Fragments arranged by size: 3489 2235 1994 1172 BsrBI CCG'CTC Cuts at: 1394 1823 6777 1394 Size: 429 4954 3507 Fragments arranged by size: 4954 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3147 5544 5875 7385 7559 65 Size: 1183 1899 2397 331 1510 174 1396 Fragments arranged by size: 2397 1899 1510 1396 1183 331 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4067 7534 7721 1700 Size: 110 2257 3467 187 2869 Fragments arranged by size: 3467 2869 2257 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8890 BssSI C'ACGA_G Cuts at: 6950 7962 6950 Size: 1012 7878 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8890 BstBI TT'CG_AA Cuts at: 7694 7694 Size: 8890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8890 BstXI CCAn_nnnn'nTGG Cuts at: 2686 5269 2686 Size: 2583 6307 BstYI r'GATC_y Cuts at: 1972 2675 2836 2897 4673 5506 6220 6587 6972 Size: 703 161 61 1776 833 714 367 385 Cuts at: 6972 6989 8441 8527 8539 8885 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1776 1452 833 714 703 385 367 346 161 86 61 17 12 BstZ17I GTA'TAC Cuts at: 4664 4664 Size: 8890 BtgI C'CryG_G Cuts at: 513 1065 5171 513 Size: 552 4106 4232 Fragments arranged by size: 4232 4106 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8151 Fragments arranged by size: 8151 683 56 BtsI GCAGTG_nn' Cuts at: 2220 2631 4578 5964 6406 7200 7220 2220 Size: 411 1947 1386 442 794 20 3890 Fragments arranged by size: 3890 1947 1386 794 442 411 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2196 2231 529 Size: 35 1632 35 7188 Fragments arranged by size: 7188 1632 35 35 DraI TTT'AAA Cuts at: 6194 6194 Size: 8890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7897 809 Size: 507 6581 1802 Fragments arranged by size: 6581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2688 3585 4866 Size: 54 1425 50 275 18 858 897 1281 Cuts at: 4866 6337 7226 8855 8 Size: 1471 889 1629 43 Fragments arranged by size: 1629 1471 1425 1281 897 889 858 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6337 1537 Size: 275 4525 4090 Fragments arranged by size: 4525 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 3147 3327 3516 3540 3639 3702 3726 Size: 705 1089 180 189 24 99 63 24 Cuts at: 3726 3915 3948 4098 4182 4230 4299 4359 4455 Size: 189 33 150 84 48 69 60 96 Cuts at: 4455 4468 4509 4548 4704 4797 5460 5985 6820 Size: 13 41 39 156 93 663 525 835 Cuts at: 6820 1353 Size: 3423 Fragments arranged by size: 3423 1089 835 705 663 525 189 189 180 156 150 99 96 93 84 69 63 60 48 41 39 33 24 24 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7477 7851 7997 387 Size: 1064 138 5888 374 146 1280 Fragments arranged by size: 5888 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2737 2737 Size: 8890 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2205 2635 3102 3348 3693 3747 3929 Size: 393 573 430 467 246 345 54 182 Cuts at: 3929 4230 4254 4380 4533 4668 4728 4950 5294 Size: 301 24 126 153 135 60 222 344 Cuts at: 5294 5484 5973 6192 6953 7550 8337 8605 1239 Size: 190 489 219 761 597 787 268 1524 Fragments arranged by size: 1524 787 761 597 573 489 467 430 393 345 344 301 268 246 222 219 190 182 153 135 126 60 54 24 EcoNI CCTnn'n_nnAGG Cuts at: 2411 3103 2411 Size: 692 8198 EcoO109I rG'GnC_Cy Cuts at: 2127 2523 2718 3222 3747 3846 4167 4440 4536 Size: 396 195 504 525 99 321 273 96 Cuts at: 4536 4632 4728 5013 2127 Size: 96 96 285 6004 Fragments arranged by size: 6004 525 504 396 321 285 273 195 99 96 96 96 EcoRI G'AATT_C Cuts at: 6199 6577 6199 Size: 378 8512 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4048 5498 4048 Size: 1450 7440 FspI TGC'GCA Cuts at: 7396 7396 Size: 8890 HaeII r_GCGC'y Cuts at: 1678 8037 1678 Size: 6359 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2275 2307 2613 Size: 32 135 32 437 32 234 32 306 Cuts at: 2613 2645 2756 2788 3284 3316 4469 4501 5213 Size: 32 111 32 496 32 1153 32 712 Cuts at: 5213 5245 5285 5317 5408 5440 5776 5808 7531 Size: 32 40 32 91 32 336 32 1723 Cuts at: 7531 7563 7605 7637 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1723 1153 712 496 437 336 306 234 135 111 91 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3529 3892 6234 6480 669 Size: 988 281 1591 363 2342 246 3079 Fragments arranged by size: 3079 2342 1591 988 363 281 246 HindIII A'AGCT_T Cuts at: 748 3018 3870 748 Size: 2270 852 5768 Fragments arranged by size: 5768 2270 852 HpaI GTT'AAC Cuts at: 6480 6480 Size: 8890 KpnI G_GTAC'C Cuts at: 6215 6215 Size: 8890 MfeI C'AATT_G Cuts at: 6489 6489 Size: 8890 MluI A'CGCG_T Cuts at: 8766 8766 Size: 8890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4061 8004 8188 1305 Size: 2756 3943 184 2007 Fragments arranged by size: 3943 2756 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2690 4868 10 Size: 54 1425 343 858 2178 4032 Fragments arranged by size: 4032 2178 1425 858 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2684 5713 6848 7207 7366 518 Size: 648 1518 3029 1135 359 159 2042 Fragments arranged by size: 3029 2042 1518 1135 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3476 3615 4385 4418 5767 6306 6983 8131 Size: 1870 139 770 33 1349 539 677 1148 Cuts at: 8131 8376 1606 Size: 245 2120 Fragments arranged by size: 2120 1870 1349 1148 770 677 539 245 139 33 NcoI C'CATG_G Cuts at: 513 1065 5171 513 Size: 552 4106 4232 Fragments arranged by size: 4232 4106 552 NdeI CA'TA_TG Cuts at: 387 5714 387 Size: 5327 3563 NheI G'CTAG_C Cuts at: 1052 3356 4411 1052 Size: 2304 1055 5531 Fragments arranged by size: 5531 2304 1055 NotI GC'GGCC_GC Cuts at: 6337 6337 Size: 8890 NspI r_CATG'y Cuts at: 1132 1229 6248 7793 1132 Size: 97 5019 1545 2229 Fragments arranged by size: 5019 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2829 1348 Size: 1481 7409 PciI A'CATG_T Cuts at: 7789 7789 Size: 8890 PflMI CCAn_nnn'nTGG Cuts at: 1108 2817 3941 1108 Size: 1709 1124 6057 Fragments arranged by size: 6057 1709 1124 PfoI T'CCnGG_A Cuts at: 3623 4976 5370 3623 Size: 1353 394 7143 Fragments arranged by size: 7143 1353 394 PmeI GTTT'AAAC Cuts at: 6194 6194 Size: 8890 PmlI CAC'GTG Cuts at: 5091 5091 Size: 8890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4808 4840 6959 6991 8472 8504 4808 Size: 32 2119 32 1481 32 5194 Fragments arranged by size: 5194 2119 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2127 2523 2718 3222 3747 3846 4167 4440 4536 Size: 396 195 504 525 99 321 273 96 Cuts at: 4536 4632 4728 5013 2127 Size: 96 96 285 6004 Fragments arranged by size: 6004 525 504 396 321 285 273 195 99 96 96 96 PshAI GACnn'nnGTC Cuts at: 1613 3980 1613 Size: 2367 6523 PsiI TTA'TAA Cuts at: 6460 6460 Size: 8890 PspOMI G'GGCC_C Cuts at: 2825 2825 Size: 8890 PstI C_TGCA'G Cuts at: 830 6242 830 Size: 5412 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8890 PvuII CAG'CTG Cuts at: 3476 3615 4385 4418 5767 3476 Size: 139 770 33 1349 6599 Fragments arranged by size: 6599 1349 770 139 33 SacI G_AGCT'C Cuts at: 2739 2739 Size: 8890 SalI G'TCGA_C Cuts at: 1936 6232 1936 Size: 4296 4594 SbfI CC_TGCA'GG Cuts at: 6242 6242 Size: 8890 ScaI AGT'ACT Cuts at: 2374 7138 8850 2374 Size: 4764 1712 2414 Fragments arranged by size: 4764 2414 1712 SexAI A'CCwGG_T Cuts at: 2816 3224 2816 Size: 408 8482 SfcI C'TryA_G Cuts at: 826 1045 3045 3237 4182 5359 5862 6238 7373 Size: 219 2000 192 945 1177 503 376 1135 Cuts at: 7373 8054 8245 826 Size: 681 191 1471 Fragments arranged by size: 2000 1471 1177 1135 945 681 503 376 219 192 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8890 SmaI CCC'GGG Cuts at: 1872 6217 1872 Size: 4345 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6994 7895 8157 820 Size: 197 926 5051 901 262 1553 Fragments arranged by size: 5051 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6681 493 Size: 6188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8890 SphI G_CATG'C Cuts at: 1132 6248 1132 Size: 5116 3774 SspI AAT'ATT Cuts at: 5 52 3741 6007 6027 6037 6088 6814 5 Size: 47 3689 2266 20 10 51 726 2081 Fragments arranged by size: 3689 2266 2081 726 51 47 20 10 StuI AGG'CCT Cuts at: 1517 2542 5128 5976 1517 Size: 1025 2586 848 4431 Fragments arranged by size: 4431 2586 1025 848 StyI C'CwwG_G Cuts at: 513 1065 2118 2172 2244 2537 3165 3374 5171 Size: 552 1053 54 72 293 628 209 1797 Cuts at: 5171 5397 6329 513 Size: 226 932 3074 Fragments arranged by size: 3074 1797 1053 932 628 552 293 226 209 72 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2207 6917 6934 7087 7272 7717 325 Size: 1255 627 4710 17 153 185 445 1498 Fragments arranged by size: 4710 1498 1255 627 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2372 5405 7136 Size: 274 80 33 51 1443 395 3033 1731 Cuts at: 7136 8848 96 Size: 1712 138 Fragments arranged by size: 3033 1731 1712 1443 395 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8850 8882 8850 Size: 32 8858 Tth111I GACn'n_nGTC Cuts at: 1316 3631 4112 1316 Size: 2315 481 6094 Fragments arranged by size: 6094 2315 481 XbaI T'CTAG_A Cuts at: 4832 6226 4832 Size: 1394 7496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2144 3286 2144 Size: 1142 7748 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8890 XmaI C'CCGG_G Cuts at: 1870 6215 1870 Size: 4345 4545 XmnI GAAnn'nnTTC Cuts at: 5499 5554 7019 5499 Size: 55 1465 7370 Fragments arranged by size: 7370 1465 55 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6698 276 Size: 53 83 186 936 5164 2468 Fragments arranged by size: 5164 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI