Restriction map for
pFN21AA0443
(Linear) (Six-base) MAPSORT of: KIAA0443.seq Check: 6425 from: 1 to: 4185
KIAA0443.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 584 1278 2658 4185
Size: 584 694 1380 1527
Fragments arranged by size:
1527 1380 694 584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1343 1742 2249 2528 2663 2723 2945
Size: 1097 246 399 507 279 135 60 222
Cuts at: 2945 3289 3479 3968 4185
Size: 344 190 489 217
Fragments arranged by size:
1097 507 489 399 344 279 246 222
217 190 135 60
AflIII A'CryG_T
Cuts at: 0 3085 4185
Size: 3085 1100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2784 4185
Size: 2784 1401
AlwNI CAG_nnn'CTG
Cuts at: 0 436 1720 2002 2317 2383 2701 3409 4185
Size: 436 1284 282 315 66 318 708 776
Fragments arranged by size:
1284 776 708 436 318 315 282 66
ApaI G_GGCC'C
Cuts at: 0 824 4185
Size: 824 3361
ApoI r'AATT_y
Cuts at: 0 634 946 3371 3494 3903 3961 4120 4185
Size: 634 312 2425 123 409 58 159 65
Fragments arranged by size:
2425 634 409 312 159 123 65 58
AseI AT'TA_AT
Cuts at: 0 3484 4185
Size: 3484 701
AvaI C'yCGr_G
Cuts at: 0 1166 3563 3629 4185
Size: 1166 2397 66 556
Fragments arranged by size:
2397 1166 556 66
AvrII C'CTAG_G
Cuts at: 0 532 4185
Size: 532 3653
BamHI G'GATC_C
Cuts at: 0 3501 4185
Size: 3501 684
BanI G'GyrC_C
Cuts at: 0 25 4185
Size: 25 4160
BanII G_rGCy'C
Cuts at: 0 288 734 824 1832 4185
Size: 288 446 90 1008 2353
Fragments arranged by size:
2353 1008 446 288 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 911 2405 4185
Size: 911 1494 1780
Fragments arranged by size:
1780 1494 911
BbvCI CC'TCA_GC
Cuts at: 0 1442 1535 1817 2003 2318 4185
Size: 1442 93 282 186 315 1867
Fragments arranged by size:
1867 1442 315 282 186 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 998 1032 4185
Size: 998 34 3153
Fragments arranged by size:
3153 998 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2066 2787 4185
Size: 2066 721 1398
Fragments arranged by size:
2066 1398 721
BclI T'GATC_A
Cuts at: 0 1109 3312 4185
Size: 1109 2203 873
Fragments arranged by size:
2203 1109 873
BlpI GC'TnA_GC
Cuts at: 0 287 4185
Size: 287 3898
Bme1580I G_kGCm'C
Cuts at: 0 30 222 824 4185
Size: 30 192 602 3361
Fragments arranged by size:
3361 602 192 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 14 1355 1445 4185
Size: 14 1341 90 2740
Fragments arranged by size:
2740 1341 90 14
BmtI G_CTAG'C
Cuts at: 0 1355 2410 4185
Size: 1355 1055 1775
Fragments arranged by size:
1775 1355 1055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 1467 4185
Size: 348 1119 2718
Fragments arranged by size:
2718 1119 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 630 1688 1924 2225 2375 4185
Size: 200 430 1058 236 301 150 1810
Fragments arranged by size:
1810 1058 430 301 236 200 150
Bpu10I CC'TnA_GC
Cuts at: 0 1442 1535 1817 2003 2318 4127 4185
Size: 1442 93 282 186 315 1809 58
Fragments arranged by size:
1809 1442 315 282 186 93 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 499 1537 2002 2230 2452 4185
Size: 499 1038 465 228 222 1733
Fragments arranged by size:
1733 1038 499 465 228 222
BsaAI yAC'GTr
Cuts at: 0 3086 4185
Size: 3086 1099
BsaWI w'CCGG_w
Cuts at: 0 2559 4185
Size: 2559 1626
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 608 638 907 937 1279 1309 1661 1691
Size: 608 30 269 30 342 30 352 30
Cuts at: 1691 4185
Size: 2494
Fragments arranged by size:
2494 608 352 342 269 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 1213 1834 1837 1840 2020 2107 2248
Size: 517 696 621 3 3 180 87 141
Cuts at: 2248 2335 2620 3151 4185
Size: 87 285 531 1034
Fragments arranged by size:
1034 696 621 531 517 285 180 141
87 87 3 3
BseYI C'CCAG_C
Cuts at: 0 967 2476 4185
Size: 967 1509 1709
Fragments arranged by size:
1709 1509 967
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 4185
Size: 227 3958
BsiHKAI G_wGCw'C
Cuts at: 0 734 4185
Size: 734 3451
BsmI GAATG_Cn'
Cuts at: 0 4102 4185
Size: 4102 83
Bsp1286I G_dGCh'C
Cuts at: 0 30 222 288 734 824 1832 4185
Size: 30 192 66 446 90 1008 2353
Fragments arranged by size:
2353 1008 446 192 90 66 30
BspEI T'CCGG_A
Cuts at: 0 2559 4185
Size: 2559 1626
BspHI T'CATG_A
Cuts at: 0 3513 3923 4185
Size: 3513 410 262
Fragments arranged by size:
3513 410 262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 833 3068 4185
Size: 833 2235 1117
Fragments arranged by size:
2235 1117 833
BsrDI GCAATG_nn'
Cuts at: 0 1142 3539 3870 4185
Size: 1142 2397 331 315
Fragments arranged by size:
2397 1142 331 315
BsrFI r'CCGG_y
Cuts at: 0 2062 4185
Size: 2062 2123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 681 3264 4185
Size: 681 2583 921
Fragments arranged by size:
2583 921 681
BstYI r'GATC_y
Cuts at: 0 670 831 892 2668 3501 4185
Size: 670 161 61 1776 833 684
Fragments arranged by size:
1776 833 684 670 161 61
BstZ17I GTA'TAC
Cuts at: 0 2659 4185
Size: 2659 1526
BtgI C'CryG_G
Cuts at: 0 3166 4185
Size: 3166 1019
BtsI GCAGTG_nn'
Cuts at: 0 215 626 2573 3959 4185
Size: 215 411 1947 1386 226
Fragments arranged by size:
1947 1386 411 226 215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 191 226 4185
Size: 191 35 3959
Fragments arranged by size:
3959 191 35
EaeI y'GGCC_r
Cuts at: 0 683 1580 2861 4185
Size: 683 897 1281 1324
Fragments arranged by size:
1324 1281 897 683
EarI CTCTTCn'nnn_
Cuts at: 0 53 1142 1322 1511 1535 1634 1697 1721
Size: 53 1089 180 189 24 99 63 24
Cuts at: 1721 1910 1943 2093 2177 2225 2294 2354 2450
Size: 189 33 150 84 48 69 60 96
Cuts at: 2450 2463 2504 2543 2699 2792 3455 3980 4185
Size: 13 41 39 156 93 663 525 205
Fragments arranged by size:
1089 663 525 205 189 189 180 156
150 99 96 93 84 69 63 60
53 48 41 39 33 24 24 13
EcoICRI GAG'CTC
Cuts at: 0 732 4185
Size: 732 3453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 630 1097 1343 1688 1742 1924 2225
Size: 200 430 467 246 345 54 182 301
Cuts at: 2225 2249 2375 2528 2663 2723 2945 3289 3479
Size: 24 126 153 135 60 222 344 190
Cuts at: 3479 3968 4185
Size: 489 217
Fragments arranged by size:
489 467 430 345 344 301 246 222
217 200 190 182 153 135 126 60
54 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 1098 4185
Size: 406 692 3087
Fragments arranged by size:
3087 692 406
EcoO109I rG'GnC_Cy
Cuts at: 0 122 518 713 1217 1742 1841 2162 2435
Size: 122 396 195 504 525 99 321 273
Cuts at: 2435 2531 2627 2723 3008 4185
Size: 96 96 96 285 1177
Fragments arranged by size:
1177 525 504 396 321 285 273 195
122 99 96 96 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2043 3493 4185
Size: 2043 1450 692
Fragments arranged by size:
2043 1450 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 270 302 608 640 751 783
Size: 4 32 234 32 306 32 111 32
Cuts at: 783 1279 1311 2464 2496 3208 3240 3280 3312
Size: 496 32 1153 32 712 32 40 32
Cuts at: 3312 3403 3435 3771 3803 4185
Size: 91 32 336 32 382
Fragments arranged by size:
1153 712 496 382 336 306 234 111
91 40 32 32 32 32 32 32
32 32 32 32 4
HincII GTy'rAC
Cuts at: 0 1524 1887 4185
Size: 1524 363 2298
Fragments arranged by size:
2298 1524 363
HindIII A'AGCT_T
Cuts at: 0 1013 1865 4185
Size: 1013 852 2320
Fragments arranged by size:
2320 1013 852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2056 4185
Size: 2056 2129
MscI TGG'CCA
Cuts at: 0 685 2863 4185
Size: 685 2178 1322
Fragments arranged by size:
2178 1322 685
MslI CAynn'nnrTG
Cuts at: 0 679 3708 4185
Size: 679 3029 477
Fragments arranged by size:
3029 679 477
MspA1I CmG'CkG
Cuts at: 0 1471 1610 2380 2413 3762 4185
Size: 1471 139 770 33 1349 423
Fragments arranged by size:
1471 1349 770 423 139 33
NcoI C'CATG_G
Cuts at: 0 3166 4185
Size: 3166 1019
NdeI CA'TA_TG
Cuts at: 0 3709 4185
Size: 3709 476
NheI G'CTAG_C
Cuts at: 0 1351 2406 4185
Size: 1351 1055 1779
Fragments arranged by size:
1779 1351 1055
PasI CC'CwG_GG
Cuts at: 0 824 4185
Size: 824 3361
PflMI CCAn_nnn'nTGG
Cuts at: 0 812 1936 4185
Size: 812 1124 2249
Fragments arranged by size:
2249 1124 812
PfoI T'CCnGG_A
Cuts at: 0 1618 2971 3365 4185
Size: 1618 1353 394 820
Fragments arranged by size:
1618 1353 820 394
PmlI CAC'GTG
Cuts at: 0 3086 4185
Size: 3086 1099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2803 2835 4185
Size: 2803 32 1350
Fragments arranged by size:
2803 1350 32
PpuMI rG'GwC_Cy
Cuts at: 0 122 518 713 1217 1742 1841 2162 2435
Size: 122 396 195 504 525 99 321 273
Cuts at: 2435 2531 2627 2723 3008 4185
Size: 96 96 96 285 1177
Fragments arranged by size:
1177 525 504 396 321 285 273 195
122 99 96 96 96
PshAI GACnn'nnGTC
Cuts at: 0 1975 4185
Size: 1975 2210
PspOMI G'GGCC_C
Cuts at: 0 820 4185
Size: 820 3365
PvuII CAG'CTG
Cuts at: 0 1471 1610 2380 2413 3762 4185
Size: 1471 139 770 33 1349 423
Fragments arranged by size:
1471 1349 770 423 139 33
SacI G_AGCT'C
Cuts at: 0 734 4185
Size: 734 3451
ScaI AGT'ACT
Cuts at: 0 369 4185
Size: 369 3816
SexAI A'CCwGG_T
Cuts at: 0 811 1219 4185
Size: 811 408 2966
Fragments arranged by size:
2966 811 408
SfcI C'TryA_G
Cuts at: 0 1040 1232 2177 3354 3857 4185
Size: 1040 192 945 1177 503 328
Fragments arranged by size:
1177 1040 945 503 328 192
SspI AAT'ATT
Cuts at: 0 1736 4002 4022 4032 4083 4185
Size: 1736 2266 20 10 51 102
Fragments arranged by size:
2266 1736 102 51 20 10
StuI AGG'CCT
Cuts at: 0 537 3123 3971 4185
Size: 537 2586 848 214
Fragments arranged by size:
2586 848 537 214
StyI C'CwwG_G
Cuts at: 0 113 167 239 532 1160 1369 3166 3392
Size: 113 54 72 293 628 209 1797 226
Cuts at: 3392 4185
Size: 793
Fragments arranged by size:
1797 793 628 293 226 209 113 72
54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 4185
Size: 202 3983
TatI w'GTAC_w
Cuts at: 0 367 3400 4185
Size: 367 3033 785
Fragments arranged by size:
3033 785 367
Tth111I GACn'n_nGTC
Cuts at: 0 1626 2107 4185
Size: 1626 481 2078
Fragments arranged by size:
2078 1626 481
XbaI T'CTAG_A
Cuts at: 0 2827 4185
Size: 2827 1358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 139 1281 4185
Size: 139 1142 2904
Fragments arranged by size:
2904 1142 139
XmnI GAAnn'nnTTC
Cuts at: 0 3494 3549 4185
Size: 3494 55 636
Fragments arranged by size:
3494 636 55
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI ApoI AseI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BstXI BstYI BstZ17I BtgI BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NdeI NheI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PvuII
SacI ScaI SexAI SfcI SspI StuI StyI TaqII
TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AsiSI BaeI BbeI BfrBI
BglI BglII BmgBI BpuEI BsaBI BsaHI BsiEI BsiWI
BsmBI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NotI NruI
NsiI NspI PacI PciI PmeI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SwaI TstI XhoI XmaI ZraI