(Circular) (Six-base) MAPSORT of: pFN21AA0439.seq Check: 6401 from: 1 to: 7207 pFN21AA0439 7207 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5017 278 Size: 53 83 186 936 3481 2468 Fragments arranged by size: 3481 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4550 1937 Size: 2613 4594 Acc65I G'GTAC_C Cuts at: 4528 4528 Size: 7207 AclI AA'CG_TT Cuts at: 1563 3272 5334 5707 1563 Size: 1709 2062 373 3063 Fragments arranged by size: 3063 2062 1709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2570 2730 3376 3644 5270 6654 6922 1632 Size: 938 160 646 268 1626 1384 268 1917 Fragments arranged by size: 1917 1626 1384 938 646 268 268 160 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7010 AflIII A'CryG_T Cuts at: 1203 2325 6106 7083 1203 Size: 1122 3781 977 1327 Fragments arranged by size: 3781 1327 1122 977 AgeI A'CCGG_T Cuts at: 6038 6038 Size: 7207 AhdI GACnn_n'nnGTC Cuts at: 3506 5936 3506 Size: 2430 4777 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7207 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7207 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3887 3919 3887 Size: 32 7175 AlwNI CAG_nnn'CTG Cuts at: 2560 4950 6522 2560 Size: 2390 1572 3245 Fragments arranged by size: 3245 2390 1572 ApaLI G'TGCA_C Cuts at: 5264 6420 5264 Size: 1156 6051 ApoI r'AATT_y Cuts at: 1493 3173 3252 3368 4104 4516 4742 4894 7003 Size: 1680 79 116 736 412 226 152 2109 Cuts at: 7003 1493 Size: 1697 Fragments arranged by size: 2109 1697 1680 736 412 226 152 116 79 AseI AT'TA_AT Cuts at: 160 1032 5761 160 Size: 872 4729 1606 Fragments arranged by size: 4729 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7207 AvaI C'yCGr_G Cuts at: 1870 1943 2061 2231 2423 2450 4532 1870 Size: 73 118 170 192 27 2082 4545 Fragments arranged by size: 4545 2082 192 170 118 73 27 BamHI G'GATC_C Cuts at: 3867 4537 4904 3867 Size: 670 367 6170 Fragments arranged by size: 6170 670 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2960 4528 5983 618 Size: 325 217 627 1173 1568 1455 1842 Fragments arranged by size: 1842 1568 1455 1173 627 325 217 BanII G_rGCy'C Cuts at: 1391 2424 2430 4399 4526 1391 Size: 1033 6 1969 127 4072 Fragments arranged by size: 4072 1969 1033 127 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2418 3318 928 Size: 971 519 900 4817 Fragments arranged by size: 4817 971 900 519 BbvCI CC'TCA_GC Cuts at: 2921 4347 2921 Size: 1426 5781 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2663 2697 5398 5432 1903 Size: 34 726 34 2701 34 3678 Fragments arranged by size: 3678 2701 726 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 5099 6309 4277 Size: 822 1210 5175 Fragments arranged by size: 5175 1210 822 BclI T'GATC_A Cuts at: 1548 3130 3283 1548 Size: 1582 153 5472 Fragments arranged by size: 5472 1582 153 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5818 136 Size: 107 122 71 631 605 4146 1525 Fragments arranged by size: 4146 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7202 7202 Size: 7207 BlpI GC'TnA_GC Cuts at: 4624 4624 Size: 7207 Bme1580I G_kGCm'C Cuts at: 1790 5268 6424 1790 Size: 3478 1156 2573 Fragments arranged by size: 3478 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2326 1318 Size: 1008 6199 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4938 5896 442 Size: 416 53 83 399 24 3521 958 1753 Fragments arranged by size: 3521 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7207 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2516 2516 Size: 7207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2511 2580 2975 3346 3893 4145 4189 5867 Size: 1272 69 395 371 547 252 44 1678 Cuts at: 5867 1239 Size: 2579 Fragments arranged by size: 2579 1678 1272 547 395 371 252 69 44 Bpu10I CC'TnA_GC Cuts at: 2772 2921 4347 2772 Size: 149 1426 5632 Fragments arranged by size: 5632 1426 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2632 4470 5332 6197 6495 2632 Size: 1838 862 865 298 3344 Fragments arranged by size: 3344 1838 865 862 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2456 5870 882 Size: 617 957 3414 2219 Fragments arranged by size: 3414 2219 957 617 BsaAI yAC'GTr Cuts at: 493 1204 4998 493 Size: 711 3794 2702 Fragments arranged by size: 3794 2702 711 BsaBI GATnn'nnATC Cuts at: 7201 7201 Size: 7207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4254 5014 5396 6031 Size: 53 83 186 936 2721 760 382 635 Cuts at: 6031 275 Size: 1451 Fragments arranged by size: 2721 1451 936 760 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2289 2858 4269 5640 6038 6312 6459 6870 2289 Size: 569 1411 1371 398 274 147 411 2626 Fragments arranged by size: 2626 1411 1371 569 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6982 Fragments arranged by size: 6982 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2205 2208 2211 2635 1186 Size: 841 178 3 3 424 5758 Fragments arranged by size: 5758 841 424 178 3 3 BseYI C'CCAG_C Cuts at: 2751 3087 3240 3331 6410 6906 2751 Size: 336 153 91 3079 496 3052 Fragments arranged by size: 3079 3052 496 336 153 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6634 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 4657 5418 6446 1540 Size: 275 186 1114 1542 761 1028 2301 Fragments arranged by size: 2301 1542 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4399 5268 5353 6424 4399 Size: 869 85 1071 5182 Fragments arranged by size: 5182 1071 869 85 BsiWI C'GTAC_G Cuts at: 2977 2977 Size: 7207 BsmI GAATG_Cn' Cuts at: 4177 4718 4811 4177 Size: 541 93 6573 Fragments arranged by size: 6573 541 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2424 2430 4399 4526 5268 5353 6424 Size: 399 634 6 1969 127 742 85 1071 Cuts at: 6424 1391 Size: 2174 Fragments arranged by size: 2174 1969 1071 742 634 399 127 85 6 BspEI T'CCGG_A Cuts at: 2858 6870 2858 Size: 4012 3195 BspHI T'CATG_A Cuts at: 5096 6826 5096 Size: 1730 5477 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3421 4562 844 Size: 2577 1141 3489 Fragments arranged by size: 3489 2577 1141 BsrBI CCG'CTC Cuts at: 1394 1823 2391 5094 1394 Size: 429 568 2703 3507 Fragments arranged by size: 3507 2703 568 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 5702 5876 65 Size: 1183 3125 1329 174 1396 Fragments arranged by size: 3125 1396 1329 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2379 2524 2907 3114 5851 6038 1700 Size: 110 569 145 383 207 2737 187 2869 Fragments arranged by size: 2869 2737 569 383 207 187 145 110 BsrGI T'GTAC_A Cuts at: 96 2722 96 Size: 2626 4581 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7207 BssSI C'ACGA_G Cuts at: 5267 6279 5267 Size: 1012 6195 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7207 BstBI TT'CG_AA Cuts at: 6011 6011 Size: 7207 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7207 BstXI CCAn_nnnn'nTGG Cuts at: 3087 3087 Size: 7207 BstYI r'GATC_y Cuts at: 1972 2537 2901 3160 3867 4537 4904 5289 5306 Size: 565 364 259 707 670 367 385 17 Cuts at: 5306 6758 6844 6856 7202 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 707 670 565 385 367 364 346 259 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2553 2553 Size: 7207 BtgI C'CryG_G Cuts at: 513 1065 2004 3642 4473 513 Size: 552 939 1638 831 3247 Fragments arranged by size: 3247 1638 939 831 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4281 487 Size: 683 56 3055 3413 Fragments arranged by size: 3413 3055 683 56 BtsI GCAGTG_nn' Cuts at: 2671 4723 5517 5537 2671 Size: 2052 794 20 4341 Fragments arranged by size: 4341 2052 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7207 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7172 DraI TTT'AAA Cuts at: 3207 4511 3207 Size: 1304 5903 DraIII CAC_nnn'GTG Cuts at: 2301 2301 Size: 7207 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2049 6214 809 Size: 507 733 4165 1802 Fragments arranged by size: 4165 1802 733 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3112 3265 3570 Size: 54 1425 50 275 18 1282 153 305 Cuts at: 3570 3738 4470 4654 5543 7172 8 Size: 168 732 184 889 1629 43 Fragments arranged by size: 1629 1425 1282 889 732 305 275 184 168 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 4654 1537 Size: 275 1300 1542 4090 Fragments arranged by size: 4090 1542 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3462 3718 5137 1353 Size: 2109 256 1419 3423 Fragments arranged by size: 3423 2109 1419 256 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2894 5794 6168 6314 387 Size: 1064 138 1305 2900 374 146 1280 Fragments arranged by size: 2900 1305 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4397 4397 Size: 7207 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2511 2570 2580 2730 2975 3346 3376 Size: 393 879 59 10 150 245 371 30 Cuts at: 3376 3644 3893 4145 4189 5270 5867 6654 6922 Size: 268 249 252 44 1081 597 787 268 Cuts at: 6922 1239 Size: 1524 Fragments arranged by size: 1524 1081 879 787 597 393 371 268 268 252 249 245 150 59 44 30 10 EcoNI CCTnn'n_nnAGG Cuts at: 2647 3675 2647 Size: 1028 6179 EcoRI G'AATT_C Cuts at: 3252 4516 4894 3252 Size: 1264 378 5565 Fragments arranged by size: 5565 1264 378 EcoRV GAT'ATC Cuts at: 3421 3421 Size: 7207 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2225 3017 2225 Size: 792 6415 FseI GG_CCGG'CC Cuts at: 3118 3118 Size: 7207 FspI TGC'GCA Cuts at: 5713 5713 Size: 7207 HaeII r_GCGC'y Cuts at: 1678 2386 3107 6354 1678 Size: 708 721 3247 2531 Fragments arranged by size: 3247 2531 721 708 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2536 2568 2840 Size: 32 135 32 743 32 189 32 272 Cuts at: 2840 2872 3492 3524 3556 3686 3718 3815 3847 Size: 32 620 32 32 130 32 97 32 Cuts at: 3847 5848 5880 5922 5954 1373 Size: 2001 32 42 32 2626 Fragments arranged by size: 2626 2001 743 620 272 189 135 130 97 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2173 2278 4551 4797 669 Size: 988 281 235 105 2273 246 3079 Fragments arranged by size: 3079 2273 988 281 246 235 105 HindIII A'AGCT_T Cuts at: 748 748 Size: 7207 HpaI GTT'AAC Cuts at: 4797 4797 Size: 7207 KpnI G_GTAC'C Cuts at: 4532 4532 Size: 7207 MfeI C'AATT_G Cuts at: 4806 4806 Size: 7207 MluI A'CGCG_T Cuts at: 7083 7083 Size: 7207 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2319 3030 6321 6505 1305 Size: 1014 711 3291 184 2007 Fragments arranged by size: 3291 2007 1014 711 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3572 10 Size: 54 1425 343 1740 3645 Fragments arranged by size: 3645 1740 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3261 4472 5165 5524 5683 518 Size: 648 2095 1211 693 359 159 2042 Fragments arranged by size: 2095 2042 1211 693 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2953 4351 4623 5300 6448 6693 1606 Size: 1347 1398 272 677 1148 245 2120 Fragments arranged by size: 2120 1398 1347 1148 677 272 245 NaeI GCC'GGC Cuts at: 2909 3116 2909 Size: 207 7000 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5716 Fragments arranged by size: 5716 939 552 NdeI CA'TA_TG Cuts at: 387 3186 4432 387 Size: 2799 1246 3162 Fragments arranged by size: 3162 2799 1246 NgoMIV G'CCGG_C Cuts at: 2907 3114 2907 Size: 207 7000 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7207 NotI GC'GGCC_GC Cuts at: 4654 4654 Size: 7207 NruI TCG'CGA Cuts at: 4300 4300 Size: 7207 NspI r_CATG'y Cuts at: 1132 1229 4407 4565 6110 1132 Size: 97 3178 158 1545 2229 Fragments arranged by size: 3178 2229 1545 158 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7207 PciI A'CATG_T Cuts at: 6106 6106 Size: 7207 PflMI CCAn_nnn'nTGG Cuts at: 1108 2108 2301 2817 3579 1108 Size: 1000 193 516 762 4736 Fragments arranged by size: 4736 1000 762 516 193 PfoI T'CCnGG_A Cuts at: 3870 3870 Size: 7207 PmeI GTTT'AAAC Cuts at: 4511 4511 Size: 7207 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5276 5308 6789 6821 5276 Size: 32 1481 32 5662 Fragments arranged by size: 5662 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7207 PsiI TTA'TAA Cuts at: 4777 4777 Size: 7207 PstI C_TGCA'G Cuts at: 830 3327 4281 4559 830 Size: 2497 954 278 3478 Fragments arranged by size: 3478 2497 954 278 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7207 PvuII CAG'CTG Cuts at: 4351 4351 Size: 7207 SacI G_AGCT'C Cuts at: 4399 4399 Size: 7207 SalI G'TCGA_C Cuts at: 1936 4549 1936 Size: 2613 4594 SbfI CC_TGCA'GG Cuts at: 4559 4559 Size: 7207 ScaI AGT'ACT Cuts at: 3635 5455 7167 3635 Size: 1820 1712 3675 Fragments arranged by size: 3675 1820 1712 SfcI C'TryA_G Cuts at: 826 1045 3323 4277 4555 5690 6371 6562 826 Size: 219 2278 954 278 1135 681 191 1471 Fragments arranged by size: 2278 1471 1135 954 681 278 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7207 SgrAI Cr'CCGG_yG Cuts at: 1700 2379 1700 Size: 679 6528 SmaI CCC'GGG Cuts at: 1872 2233 4534 1872 Size: 361 2301 4545 Fragments arranged by size: 4545 2301 361 SmlI C'TyrA_G Cuts at: 820 1017 1943 2061 2647 4485 5311 6212 6474 Size: 197 926 118 586 1838 826 901 262 Cuts at: 6474 820 Size: 1553 Fragments arranged by size: 1838 1553 926 901 826 586 262 197 118 SnaBI TAC'GTA Cuts at: 493 4998 493 Size: 4505 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7207 SphI G_CATG'C Cuts at: 1132 4565 1132 Size: 3433 3774 SspI AAT'ATT Cuts at: 5 52 3848 5131 5 Size: 47 3796 1283 2081 Fragments arranged by size: 3796 2081 1283 47 StuI AGG'CCT Cuts at: 1517 3681 1517 Size: 2164 5043 StyI C'CwwG_G Cuts at: 513 1065 2004 2456 2964 3220 4646 513 Size: 552 939 452 508 256 1426 3074 Fragments arranged by size: 3074 1426 939 552 508 452 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2492 5234 5251 5404 5589 6034 325 Size: 1255 912 2742 17 153 185 445 1498 Fragments arranged by size: 2742 1498 1255 912 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2722 3181 3633 Size: 274 80 33 51 1443 745 459 452 Cuts at: 3633 5453 7165 96 Size: 1820 1712 138 Fragments arranged by size: 1820 1712 1443 745 459 452 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7167 7199 7167 Size: 32 7175 Tth111I GACn'n_nGTC Cuts at: 1316 4010 1316 Size: 2694 4513 XbaI T'CTAG_A Cuts at: 4543 4543 Size: 7207 XhoI C'TCGA_G Cuts at: 1943 2061 1943 Size: 118 7089 XmaI C'CCGG_G Cuts at: 1870 2231 4532 1870 Size: 361 2301 4545 Fragments arranged by size: 4545 2301 361 XmnI GAAnn'nnTTC Cuts at: 3252 4058 4083 5336 3252 Size: 806 25 1253 5123 Fragments arranged by size: 5123 1253 806 25 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5015 276 Size: 53 83 186 936 3481 2468 Fragments arranged by size: 3481 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsmBI BstZ17I EcoO109I FspAI KasI NarI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI