(Circular) (Six-base) MAPSORT of: pFN21AA0437.seq Check: 4182 from: 1 to: 9493 pFN21AA0437 9493 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5361 5361 Size: 9493 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7303 278 Size: 53 83 186 936 5767 2468 Fragments arranged by size: 5767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5699 6836 1937 Size: 3762 1137 4594 Fragments arranged by size: 4594 3762 1137 Acc65I G'GTAC_C Cuts at: 6814 6814 Size: 9493 AclI AA'CG_TT Cuts at: 1563 7620 7993 1563 Size: 6057 373 3063 Fragments arranged by size: 6057 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 2921 4094 5019 6209 6326 7556 8940 Size: 701 588 1173 925 1190 117 1230 1384 Cuts at: 8940 9208 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1230 1190 1173 925 701 588 268 117 AflII C'TTAA_G Cuts at: 820 1017 2023 820 Size: 197 1006 8290 Fragments arranged by size: 8290 1006 197 AflIII A'CryG_T Cuts at: 1203 2820 5841 8392 9369 1203 Size: 1617 3021 2551 977 1327 Fragments arranged by size: 3021 2551 1617 1327 977 AgeI A'CCGG_T Cuts at: 2609 8324 2609 Size: 5715 3778 AhdI GACnn_n'nnGTC Cuts at: 5044 5695 8222 5044 Size: 651 2527 6315 Fragments arranged by size: 6315 2527 651 AleI CACnn'nnGTG Cuts at: 1166 4528 1166 Size: 3362 6131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6461 1184 Size: 5277 4216 AlwNI CAG_nnn'CTG Cuts at: 2135 2726 4864 7236 8808 2135 Size: 591 2138 2372 1572 2820 Fragments arranged by size: 2820 2372 2138 1572 591 ApaLI G'TGCA_C Cuts at: 3529 7550 8706 3529 Size: 4021 1156 4316 Fragments arranged by size: 4316 4021 1156 ApoI r'AATT_y Cuts at: 1493 2273 2359 3578 3944 6802 7028 7180 9289 Size: 780 86 1219 366 2858 226 152 2109 Cuts at: 9289 1493 Size: 1697 Fragments arranged by size: 2858 2109 1697 1219 780 366 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 4143 4968 8047 160 Size: 872 3111 825 3079 1606 Fragments arranged by size: 3111 3079 1606 872 825 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9493 AvaI C'yCGr_G Cuts at: 1870 1943 6552 6648 6765 6818 1870 Size: 73 4609 96 117 53 4545 Fragments arranged by size: 4609 4545 117 96 73 53 AvrII C'CTAG_G Cuts at: 5494 5494 Size: 9493 BamHI G'GATC_C Cuts at: 2145 3238 3385 5490 6823 7190 2145 Size: 1093 147 2105 1333 367 4448 Fragments arranged by size: 4448 2105 1333 1093 367 147 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3226 4990 5098 6559 6814 Size: 325 217 627 1439 1764 108 1461 255 Cuts at: 6814 8269 618 Size: 1455 1842 Fragments arranged by size: 1842 1764 1461 1455 1439 627 325 255 217 108 BanII G_rGCy'C Cuts at: 1391 2772 2979 3678 4379 4661 5595 6482 6498 Size: 1381 207 699 701 282 934 887 16 Cuts at: 6498 6812 1391 Size: 314 4072 Fragments arranged by size: 4072 1381 934 887 701 699 314 282 207 16 BbeI G_GCGC'C Cuts at: 6563 6563 Size: 9493 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3992 4019 4308 5490 928 Size: 971 2093 27 289 1182 4931 Fragments arranged by size: 4931 2093 1182 971 289 27 BbvCI CC'TCA_GC Cuts at: 2074 2074 Size: 9493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6147 6181 6331 6365 7684 7718 1903 Size: 34 4210 34 150 34 1319 34 3678 Fragments arranged by size: 4210 3678 1319 150 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3348 7385 8595 3348 Size: 4037 1210 4246 Fragments arranged by size: 4246 4037 1210 BclI T'GATC_A Cuts at: 1548 3741 4975 1548 Size: 2193 1234 6066 Fragments arranged by size: 6066 2193 1234 BfrBI ATG'CAT Cuts at: 6438 6438 Size: 9493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3140 6557 8104 Size: 107 122 71 631 605 1468 3417 1547 Cuts at: 8104 136 Size: 1525 Fragments arranged by size: 3417 1547 1525 1468 631 605 122 107 71 BglII A'GATC_T Cuts at: 2851 4044 9488 2851 Size: 1193 5444 2856 Fragments arranged by size: 5444 2856 1193 BlpI GC'TnA_GC Cuts at: 5325 6687 6910 5325 Size: 1362 223 7908 Fragments arranged by size: 7908 1362 223 Bme1580I G_kGCm'C Cuts at: 1790 3136 3533 6072 7554 8710 1790 Size: 1346 397 2539 1482 1156 2573 Fragments arranged by size: 2573 2539 1482 1346 1156 397 BmgBI CAC'GTC Cuts at: 1318 5842 5887 1318 Size: 4524 45 4924 Fragments arranged by size: 4924 4524 45 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4139 5127 6125 Size: 416 53 83 399 24 2722 988 998 Cuts at: 6125 6385 7224 8182 442 Size: 260 839 958 1753 Fragments arranged by size: 2722 1753 998 988 958 839 416 399 260 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9493 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3448 4558 4675 3448 Size: 1110 117 8266 Fragments arranged by size: 8266 1110 117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2112 3692 3732 4412 4841 4895 5533 5985 Size: 873 1580 40 680 429 54 638 452 Cuts at: 5985 6391 6512 8153 1239 Size: 406 121 1641 2579 Fragments arranged by size: 2579 1641 1580 873 680 638 452 429 406 121 54 40 Bpu10I CC'TnA_GC Cuts at: 2074 2197 4373 5561 6476 2074 Size: 123 2176 1188 915 5091 Fragments arranged by size: 5091 2176 1188 915 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2786 3178 4004 4787 5776 7618 8483 8781 2786 Size: 392 826 783 989 1842 865 298 3498 Fragments arranged by size: 3498 1842 989 865 826 783 392 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2074 4088 6405 8156 882 Size: 617 575 2014 2317 1751 2219 Fragments arranged by size: 2317 2219 2014 1751 617 575 BsaAI yAC'GTr Cuts at: 493 1204 2823 6292 7284 493 Size: 711 1619 3469 992 2702 Fragments arranged by size: 3469 2702 1619 992 711 BsaBI GATnn'nnATC Cuts at: 2393 2399 3212 9487 2393 Size: 6 813 6275 2399 Fragments arranged by size: 6275 2399 813 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4313 5718 6370 6560 Size: 53 83 186 936 2780 1405 652 190 Cuts at: 6560 7300 7682 8317 275 Size: 740 382 635 1451 Fragments arranged by size: 2780 1451 1405 936 740 652 635 382 190 186 83 53 BsaWI w'CCGG_w Cuts at: 2609 3204 4917 5315 5487 7926 8324 8598 8745 Size: 595 1713 398 172 2439 398 274 147 Cuts at: 8745 9156 2609 Size: 411 2946 Fragments arranged by size: 2946 2439 1713 595 411 398 398 274 172 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3665 3695 5282 5312 1178 Size: 30 165 30 2262 30 1587 30 5359 Fragments arranged by size: 5359 2262 1587 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2062 2192 2195 2483 2976 4604 4892 5920 Size: 876 130 3 288 493 1628 288 1028 Cuts at: 5920 5980 6172 6749 1186 Size: 60 192 577 3930 Fragments arranged by size: 3930 1628 1028 876 577 493 288 288 192 130 60 3 BseYI C'CCAG_C Cuts at: 2086 2909 3903 4601 4952 5212 5721 6449 6470 Size: 823 994 698 351 260 509 728 21 Cuts at: 6470 8696 9192 2086 Size: 2226 496 2387 Fragments arranged by size: 2387 2226 994 823 728 698 509 496 351 260 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2124 2832 5340 1163 Size: 573 388 708 2508 5316 Fragments arranged by size: 5316 2508 708 573 388 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6943 7704 8732 1540 Size: 275 186 4942 761 1028 2301 Fragments arranged by size: 4942 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3533 4444 4661 5595 7554 7639 8710 3533 Size: 911 217 934 1959 85 1071 4316 Fragments arranged by size: 4316 1959 1071 934 911 217 85 BsmI GAATG_Cn' Cuts at: 3079 5166 7004 7097 3079 Size: 2087 1838 93 5475 Fragments arranged by size: 5475 2087 1838 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3794 4586 3794 Size: 792 8701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2772 2979 3136 3533 3678 4379 4444 Size: 399 982 207 157 397 145 701 65 Cuts at: 4444 4661 5595 6072 6482 6498 6812 7554 7639 Size: 217 934 477 410 16 314 742 85 Cuts at: 7639 8710 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 982 934 742 701 477 410 399 397 314 217 207 157 145 85 65 16 BspEI T'CCGG_A Cuts at: 3204 9156 3204 Size: 5952 3541 BspHI T'CATG_A Cuts at: 3513 3808 5259 7382 9112 3513 Size: 295 1451 2123 1730 3894 Fragments arranged by size: 3894 2123 1730 1451 295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5361 6453 6848 844 Size: 4517 1092 395 3489 Fragments arranged by size: 4517 3489 1092 395 BsrBI CCG'CTC Cuts at: 1394 1823 2762 5569 6686 7380 1394 Size: 429 939 2807 1117 694 3507 Fragments arranged by size: 3507 2807 1117 939 694 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7988 8162 65 Size: 1183 6740 174 1396 Fragments arranged by size: 6740 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 2609 4743 4755 5654 6421 8137 Size: 110 255 544 2134 12 899 767 1716 Cuts at: 8137 8324 1700 Size: 187 2869 Fragments arranged by size: 2869 2134 1716 899 767 544 255 187 110 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9493 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9493 BssSI C'ACGA_G Cuts at: 4937 5821 7553 8565 4937 Size: 884 1732 1012 5865 Fragments arranged by size: 5865 1732 1012 884 BstAPI GCAn_nnn'nTGC Cuts at: 794 3131 3787 794 Size: 2337 656 6500 Fragments arranged by size: 6500 2337 656 BstBI TT'CG_AA Cuts at: 5864 8297 5864 Size: 2433 7060 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9493 BstXI CCAn_nnnn'nTGG Cuts at: 4507 4507 Size: 9493 BstYI r'GATC_y Cuts at: 1972 2145 2754 2851 3238 3385 4044 5490 6261 Size: 173 609 97 387 147 659 1446 771 Cuts at: 6261 6300 6823 7190 7575 7592 9044 9130 9142 Size: 39 523 367 385 17 1452 86 12 Cuts at: 9142 9488 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1446 771 659 609 523 387 385 367 346 173 147 97 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2772 3494 3507 513 Size: 552 939 768 722 13 6499 Fragments arranged by size: 6499 939 768 722 552 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4863 487 Size: 683 56 3637 5117 Fragments arranged by size: 5117 3637 683 56 BtsI GCAGTG_nn' Cuts at: 2815 3343 5425 5767 5806 6048 7009 7803 7823 Size: 528 2082 342 39 242 961 794 20 Cuts at: 7823 2815 Size: 4485 Fragments arranged by size: 4485 2082 961 794 528 342 242 39 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5497 5532 529 Size: 35 4933 35 4490 Fragments arranged by size: 4933 4490 35 35 DraI TTT'AAA Cuts at: 6797 6797 Size: 9493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8500 809 Size: 507 7184 1802 Fragments arranged by size: 7184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3648 4509 4750 Size: 54 1425 50 275 18 1818 861 241 Cuts at: 4750 5656 6522 6940 7829 9458 8 Size: 906 866 418 889 1629 43 Fragments arranged by size: 1818 1629 1425 906 889 866 861 418 275 241 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6940 1537 Size: 275 5128 4090 Fragments arranged by size: 5128 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 4449 4649 6188 6353 6726 7423 1353 Size: 906 2190 200 1539 165 373 697 3423 Fragments arranged by size: 3423 2190 1539 906 697 373 200 165 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2172 6669 8080 8454 8600 387 Size: 1064 138 583 4497 1411 374 146 1280 Fragments arranged by size: 4497 1411 1280 1064 583 374 146 138 EcoICRI GAG'CTC Cuts at: 4659 5593 4659 Size: 934 8559 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2333 2921 3692 3732 4094 4412 Size: 393 480 221 588 771 40 362 318 Cuts at: 4412 4841 4895 5019 5533 5985 6209 6326 6391 Size: 429 54 124 514 452 224 117 65 Cuts at: 6391 6512 7556 8153 8940 9208 1239 Size: 121 1044 597 787 268 1524 Fragments arranged by size: 1524 1044 787 771 597 588 514 480 452 429 393 362 318 268 224 221 124 121 117 65 54 40 EcoNI CCTnn'n_nnAGG Cuts at: 2597 6725 2597 Size: 4128 5365 EcoO109I rG'GnC_Cy Cuts at: 2132 2556 5360 5499 6679 6784 2132 Size: 424 2804 139 1180 105 4841 Fragments arranged by size: 4841 2804 1180 424 139 105 EcoRI G'AATT_C Cuts at: 6802 7180 6802 Size: 378 9115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3618 5695 3618 Size: 2077 7416 FspI TGC'GCA Cuts at: 7999 7999 Size: 9493 HaeII r_GCGC'y Cuts at: 1678 6563 8640 1678 Size: 4885 2077 2531 Fragments arranged by size: 4885 2531 2077 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 2746 2778 3410 Size: 32 135 32 632 32 510 32 632 Cuts at: 3410 3442 3665 3697 4679 4711 5030 5062 5681 Size: 32 223 32 982 32 319 32 619 Cuts at: 5681 5713 5998 6030 6389 6421 8134 8166 8208 Size: 32 285 32 359 32 1713 32 42 Cuts at: 8208 8240 1373 Size: 32 2626 Fragments arranged by size: 2626 1713 982 632 632 619 510 359 319 285 223 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3349 6106 6837 7083 669 Size: 988 281 1411 2757 731 246 3079 Fragments arranged by size: 3079 2757 1411 988 731 281 246 HindIII A'AGCT_T Cuts at: 748 5664 748 Size: 4916 4577 HpaI GTT'AAC Cuts at: 3349 7083 3349 Size: 3734 5759 KasI G'GCGC_C Cuts at: 6559 6559 Size: 9493 KpnI G_GTAC'C Cuts at: 6818 6818 Size: 9493 MfeI C'AATT_G Cuts at: 7092 7092 Size: 9493 MluI A'CGCG_T Cuts at: 9369 9369 Size: 9493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 2247 3266 3387 3548 3940 3957 4317 Size: 936 6 1019 121 161 392 17 360 Cuts at: 4317 4472 4749 5739 6147 8607 8791 1305 Size: 155 277 990 408 2460 184 2007 Fragments arranged by size: 2460 2007 1019 990 936 408 392 360 277 184 161 155 121 17 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 3650 4511 4752 6524 10 Size: 54 1425 343 1818 861 241 1772 2979 Fragments arranged by size: 2979 1818 1772 1425 861 343 241 54 MslI CAynn'nnrTG Cuts at: 518 1166 3512 4528 5174 5309 6077 6518 6545 Size: 648 2346 1016 646 135 768 441 27 Cuts at: 6545 7451 7810 7969 518 Size: 906 359 159 2042 Fragments arranged by size: 2346 2042 1016 906 768 648 646 441 359 159 135 27 MspA1I CmG'CkG Cuts at: 1606 2090 2688 3496 4611 5599 6484 6909 7586 Size: 484 598 808 1115 988 885 425 677 Cuts at: 7586 8734 8979 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1115 988 885 808 677 598 484 425 245 NaeI GCC'GGC Cuts at: 5656 5656 Size: 9493 NarI GG'CG_CC Cuts at: 6560 6560 Size: 9493 NcoI C'CATG_G Cuts at: 513 1065 2004 3507 513 Size: 552 939 1503 6499 Fragments arranged by size: 6499 1503 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9493 NgoMIV G'CCGG_C Cuts at: 5654 5654 Size: 9493 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9493 NotI GC'GGCC_GC Cuts at: 6940 6940 Size: 9493 NsiI A_TGCA'T Cuts at: 6440 6440 Size: 9493 NspI r_CATG'y Cuts at: 1132 1229 5389 6851 8396 1132 Size: 97 4160 1462 1545 2229 Fragments arranged by size: 4160 2229 1545 1462 97 PasI CC'CwG_GG Cuts at: 1348 2794 4103 1348 Size: 1446 1309 6738 Fragments arranged by size: 6738 1446 1309 PciI A'CATG_T Cuts at: 8392 8392 Size: 9493 PflMI CCAn_nnn'nTGG Cuts at: 1108 2135 2726 4864 1108 Size: 1027 591 2138 5737 Fragments arranged by size: 5737 2138 1027 591 PfoI T'CCnGG_A Cuts at: 2128 2128 Size: 9493 PmeI GTTT'AAAC Cuts at: 6797 6797 Size: 9493 PmlI CAC'GTG Cuts at: 2823 6292 2823 Size: 3469 6024 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2103 2135 6047 6079 7562 7594 9075 9107 2103 Size: 32 3912 32 1483 32 1481 32 2489 Fragments arranged by size: 3912 2489 1483 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2132 5360 5499 6784 2132 Size: 3228 139 1285 4841 Fragments arranged by size: 4841 3228 1285 139 PshAI GACnn'nnGTC Cuts at: 1613 3195 1613 Size: 1582 7911 PsiI TTA'TAA Cuts at: 7063 7063 Size: 9493 PstI C_TGCA'G Cuts at: 830 5358 6160 6324 6845 830 Size: 4528 802 164 521 3478 Fragments arranged by size: 4528 3478 802 521 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9493 PvuII CAG'CTG Cuts at: 2090 2090 Size: 9493 SacI G_AGCT'C Cuts at: 4661 5595 4661 Size: 934 8559 SacII CC_GC'GG Cuts at: 3497 3497 Size: 9493 SalI G'TCGA_C Cuts at: 1936 6835 1936 Size: 4899 4594 SanDI GG'GwC_CC Cuts at: 5499 5499 Size: 9493 SapI GCTCTTCn'nnn_ Cuts at: 2259 4449 4649 2259 Size: 2190 200 7103 Fragments arranged by size: 7103 2190 200 SbfI CC_TGCA'GG Cuts at: 5358 6845 5358 Size: 1487 8006 ScaI AGT'ACT Cuts at: 7741 9453 7741 Size: 1712 7781 SfcI C'TryA_G Cuts at: 826 1045 5354 6156 6320 6841 7976 8657 8848 Size: 219 4309 802 164 521 1135 681 191 Cuts at: 8848 826 Size: 1471 Fragments arranged by size: 4309 1471 1135 802 681 521 219 191 164 SfoI GGC'GCC Cuts at: 6561 6561 Size: 9493 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9493 SgrAI Cr'CCGG_yG Cuts at: 1700 2609 1700 Size: 909 8584 SmaI CCC'GGG Cuts at: 1872 6554 6820 1872 Size: 4682 266 4545 Fragments arranged by size: 4682 4545 266 SmlI C'TyrA_G Cuts at: 820 1017 1943 2023 2765 3193 4019 4802 5755 Size: 197 926 80 742 428 826 783 953 Cuts at: 5755 7597 8498 8760 820 Size: 1842 901 262 1553 Fragments arranged by size: 1842 1553 953 926 901 826 783 742 428 262 197 80 SnaBI TAC'GTA Cuts at: 493 7284 493 Size: 6791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9493 SphI G_CATG'C Cuts at: 1132 6851 1132 Size: 5719 3774 SspI AAT'ATT Cuts at: 5 52 7417 5 Size: 47 7365 2081 Fragments arranged by size: 7365 2081 47 StuI AGG'CCT Cuts at: 1517 4255 5068 1517 Size: 2738 813 5942 Fragments arranged by size: 5942 2738 813 StyI C'CwwG_G Cuts at: 513 1065 2004 2943 3312 3507 5191 5377 5409 Size: 552 939 939 369 195 1684 186 32 Cuts at: 5409 5494 5739 6932 513 Size: 85 245 1193 3074 Fragments arranged by size: 3074 1684 1193 939 939 552 369 245 195 186 85 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2233 2923 3520 4350 4513 6404 7520 Size: 1255 653 690 597 830 163 1891 1116 Cuts at: 7520 7537 7690 7875 8320 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1891 1498 1255 1116 830 690 653 597 445 185 163 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5347 7739 9451 Size: 274 80 33 51 1443 3370 2392 1712 Cuts at: 9451 96 Size: 138 Fragments arranged by size: 3370 2392 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3530 3562 5271 5282 5303 5314 9453 9485 3530 Size: 32 1709 11 21 11 4139 32 3538 Fragments arranged by size: 4139 3538 1709 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9493 XbaI T'CTAG_A Cuts at: 6829 6829 Size: 9493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3060 3280 6280 3060 Size: 220 3000 6273 Fragments arranged by size: 6273 3000 220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9493 XmaI C'CCGG_G Cuts at: 1870 6552 6818 1870 Size: 4682 266 4545 Fragments arranged by size: 4682 4545 266 XmnI GAAnn'nnTTC Cuts at: 2708 6197 7622 2708 Size: 3489 1425 4579 Fragments arranged by size: 4579 3489 1425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7301 276 Size: 53 83 186 936 5767 2468 Fragments arranged by size: 5767 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI PacI PspOMI PspXI PsrI RsrII SexAI SfiI SrfI SwaI