(Circular) (Six-base) MAPSORT of: pFN21AA0431.seq Check: 1647 from: 1 to: 6706 pFN21AA0431 6706 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4516 278 Size: 53 83 186 936 2980 2468 Fragments arranged by size: 2980 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4049 1937 Size: 2112 4594 Acc65I G'GTAC_C Cuts at: 4027 4027 Size: 6706 AclI AA'CG_TT Cuts at: 1563 4833 5206 1563 Size: 3270 373 3063 Fragments arranged by size: 3270 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2307 3510 3783 4769 6153 6421 1632 Size: 675 1203 273 986 1384 268 1917 Fragments arranged by size: 1917 1384 1203 986 675 273 268 AflII C'TTAA_G Cuts at: 820 1017 2621 820 Size: 197 1604 4905 Fragments arranged by size: 4905 1604 197 AflIII A'CryG_T Cuts at: 1203 2739 2741 5605 6582 1203 Size: 1536 2 2864 977 1327 Fragments arranged by size: 2864 1536 1327 977 2 AgeI A'CCGG_T Cuts at: 5537 5537 Size: 6706 AhdI GACnn_n'nnGTC Cuts at: 5435 5435 Size: 6706 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6706 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6706 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3935 3967 3935 Size: 32 6674 AlwNI CAG_nnn'CTG Cuts at: 2555 4449 6021 2555 Size: 1894 1572 3240 Fragments arranged by size: 3240 1894 1572 ApaLI G'TGCA_C Cuts at: 2113 2235 2558 3243 4763 5919 2113 Size: 122 323 685 1520 1156 2900 Fragments arranged by size: 2900 1520 1156 685 323 122 ApoI r'AATT_y Cuts at: 1493 3235 3607 4015 4241 4393 6502 1493 Size: 1742 372 408 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 408 372 226 152 AseI AT'TA_AT Cuts at: 160 1032 5260 160 Size: 872 4228 1606 Fragments arranged by size: 4228 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6706 AvaI C'yCGr_G Cuts at: 1870 1943 4031 1870 Size: 73 2088 4545 Fragments arranged by size: 4545 2088 73 BamHI G'GATC_C Cuts at: 4036 4403 4036 Size: 367 6339 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4027 5482 618 Size: 325 217 627 2240 1455 1842 Fragments arranged by size: 2240 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2543 4025 1391 Size: 1152 1482 4072 Fragments arranged by size: 4072 1482 1152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2358 928 Size: 971 459 5276 Fragments arranged by size: 5276 971 459 BbvCI CC'TCA_GC Cuts at: 2506 2506 Size: 6706 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2168 2202 4897 4931 1903 Size: 34 231 34 2695 34 3678 Fragments arranged by size: 3678 2695 231 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4598 5808 4598 Size: 1210 5496 BclI T'GATC_A Cuts at: 1548 2533 1548 Size: 985 5721 BfrBI ATG'CAT Cuts at: 2014 2969 2014 Size: 955 5751 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2552 5317 136 Size: 107 122 71 631 605 880 2765 1525 Fragments arranged by size: 2765 1525 880 631 605 122 107 71 BglII A'GATC_T Cuts at: 2782 6701 2782 Size: 3919 2787 BlpI GC'TnA_GC Cuts at: 4123 4123 Size: 6706 Bme1580I G_kGCm'C Cuts at: 1790 2117 2178 2239 2562 3247 4767 5923 1790 Size: 327 61 61 323 685 1520 1156 2573 Fragments arranged by size: 2573 1520 1156 685 327 323 61 61 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6706 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2181 4437 5395 Size: 416 53 83 399 24 764 2256 958 Cuts at: 5395 442 Size: 1753 Fragments arranged by size: 2256 1753 958 764 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6706 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2424 2632 2652 2424 Size: 208 20 6478 Fragments arranged by size: 6478 208 20 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2989 3700 5366 1239 Size: 1750 711 1666 2579 Fragments arranged by size: 2579 1750 1666 711 Bpu10I CC'TnA_GC Cuts at: 2506 2506 Size: 6706 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2292 2868 3647 4831 5696 5994 2292 Size: 576 779 1184 865 298 3004 Fragments arranged by size: 3004 1184 865 779 576 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3007 3746 5369 882 Size: 617 1508 739 1623 2219 Fragments arranged by size: 2219 1623 1508 739 617 BsaAI yAC'GTr Cuts at: 493 1204 2742 4497 493 Size: 711 1538 1755 2702 Fragments arranged by size: 2702 1755 1538 711 BsaBI GATnn'nnATC Cuts at: 6700 6700 Size: 6706 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4513 4895 5530 275 Size: 53 83 186 936 2980 382 635 1451 Fragments arranged by size: 2980 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2109 5139 5537 5811 5958 6369 2109 Size: 3030 398 274 147 411 2446 Fragments arranged by size: 3030 2446 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3935 3965 3980 4010 1178 Size: 30 165 30 2532 30 15 30 3874 Fragments arranged by size: 3874 2532 165 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2494 1186 Size: 1308 5398 BseYI C'CCAG_C Cuts at: 2511 2927 3402 3418 5909 6405 2511 Size: 416 475 16 2491 496 2812 Fragments arranged by size: 2812 2491 496 475 416 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2065 2795 2802 3207 1163 Size: 573 329 730 7 405 4662 Fragments arranged by size: 4662 730 573 405 329 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4156 4917 5945 1540 Size: 275 186 2155 761 1028 2301 Fragments arranged by size: 2301 2155 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2117 2239 2562 3247 4767 4852 5923 2117 Size: 122 323 685 1520 85 1071 2900 Fragments arranged by size: 2900 1520 1071 685 323 122 85 BsiWI C'GTAC_G Cuts at: 2055 2055 Size: 6706 BsmI GAATG_Cn' Cuts at: 4217 4310 4217 Size: 93 6613 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2178 2239 2543 2562 3247 4025 Size: 399 327 61 61 304 19 685 778 Cuts at: 4025 4767 4852 5923 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 778 742 685 399 327 304 85 61 61 19 BspEI T'CCGG_A Cuts at: 6369 6369 Size: 6706 BspHI T'CATG_A Cuts at: 4595 6325 4595 Size: 1730 4976 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2194 3799 4061 844 Size: 1350 1605 262 3489 Fragments arranged by size: 3489 1605 1350 262 BsrBI CCG'CTC Cuts at: 1394 1823 4593 1394 Size: 429 2770 3507 Fragments arranged by size: 3507 2770 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3906 5201 5375 65 Size: 1183 2658 1295 174 1396 Fragments arranged by size: 2658 1396 1295 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5350 5537 1700 Size: 110 3540 187 2869 Fragments arranged by size: 3540 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3052 96 Size: 2956 3750 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6706 BssSI C'ACGA_G Cuts at: 2177 4766 5778 2177 Size: 2589 1012 3105 Fragments arranged by size: 3105 2589 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2566 794 Size: 1772 4934 BstBI TT'CG_AA Cuts at: 5510 5510 Size: 6706 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6706 BstXI CCAn_nnnn'nTGG Cuts at: 2996 2996 Size: 6706 BstYI r'GATC_y Cuts at: 1972 2782 4036 4403 4788 4805 6257 6343 6355 Size: 810 1254 367 385 17 1452 86 12 Cuts at: 6355 6701 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1254 810 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6154 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5967 Fragments arranged by size: 5967 683 56 BtsI GCAGTG_nn' Cuts at: 2147 2829 3774 4222 5016 5036 2147 Size: 682 945 448 794 20 3817 Fragments arranged by size: 3817 945 794 682 448 20 ClaI AT'CG_AT Cuts at: 1554 3438 1554 Size: 1884 4822 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6671 DraI TTT'AAA Cuts at: 3201 4010 3201 Size: 809 5897 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2785 5713 809 Size: 507 1469 2928 1802 Fragments arranged by size: 2928 1802 1469 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4153 5042 6671 Size: 54 1425 50 275 18 2323 889 1629 Cuts at: 6671 8 Size: 43 Fragments arranged by size: 2323 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4153 1537 Size: 275 2341 4090 Fragments arranged by size: 4090 2341 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2898 3447 3486 4636 1353 Size: 1545 549 39 1150 3423 Fragments arranged by size: 3423 1545 1150 549 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5293 5667 5813 387 Size: 1064 138 3704 374 146 1280 Fragments arranged by size: 3704 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2989 3510 3700 3783 4769 5366 Size: 393 675 682 521 190 83 986 597 Cuts at: 5366 6153 6421 1239 Size: 787 268 1524 Fragments arranged by size: 1524 986 787 682 675 597 521 393 268 190 83 EcoO109I rG'GnC_Cy Cuts at: 2071 2200 2071 Size: 129 6577 EcoRI G'AATT_C Cuts at: 4015 4393 4015 Size: 378 6328 EcoRV GAT'ATC Cuts at: 2405 3448 2405 Size: 1043 5663 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2909 2909 Size: 6706 FspI TGC'GCA Cuts at: 5212 5212 Size: 6706 HaeII r_GCGC'y Cuts at: 1678 5853 1678 Size: 4175 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2525 2557 3019 3051 3148 Size: 32 135 32 953 32 462 32 97 Cuts at: 3148 3180 3187 3219 3268 3300 3343 3375 3931 Size: 32 7 32 49 32 43 32 556 Cuts at: 3931 3963 5347 5379 5421 5453 1373 Size: 32 1384 32 42 32 2626 Fragments arranged by size: 2626 1384 953 556 462 135 97 49 43 42 32 32 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 4050 4296 669 Size: 988 281 2112 246 3079 Fragments arranged by size: 3079 2112 988 281 246 HindIII A'AGCT_T Cuts at: 748 2319 748 Size: 1571 5135 HpaI GTT'AAC Cuts at: 4296 4296 Size: 6706 KpnI G_GTAC'C Cuts at: 4031 4031 Size: 6706 MfeI C'AATT_G Cuts at: 4305 4305 Size: 6706 MluI A'CGCG_T Cuts at: 6582 6582 Size: 6706 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 3062 4023 5820 6004 1305 Size: 975 782 961 1797 184 2007 Fragments arranged by size: 2007 1797 975 961 782 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4884 Fragments arranged by size: 4884 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4664 5023 5182 518 Size: 648 843 2655 359 159 2042 Fragments arranged by size: 2655 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4122 4799 5947 6192 1606 Size: 2516 677 1148 245 2120 Fragments arranged by size: 2516 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6154 NdeI CA'TA_TG Cuts at: 387 387 Size: 6706 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6706 NotI GC'GGCC_GC Cuts at: 4153 4153 Size: 6706 NsiI A_TGCA'T Cuts at: 2016 2971 2016 Size: 955 5751 NspI r_CATG'y Cuts at: 1132 1229 2018 2087 2121 4064 5609 1132 Size: 97 789 69 34 1943 1545 2229 Fragments arranged by size: 2229 1943 1545 789 97 69 34 PasI CC'CwG_GG Cuts at: 1348 3859 1348 Size: 2511 4195 PciI A'CATG_T Cuts at: 5605 5605 Size: 6706 PflMI CCAn_nnn'nTGG Cuts at: 1108 2518 3355 1108 Size: 1410 837 4459 Fragments arranged by size: 4459 1410 837 PfoI T'CCnGG_A Cuts at: 3388 3388 Size: 6706 PmeI GTTT'AAAC Cuts at: 4010 4010 Size: 6706 PmlI CAC'GTG Cuts at: 2742 2742 Size: 6706 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 4775 4807 6288 6320 3935 Size: 32 808 32 1481 32 4321 Fragments arranged by size: 4321 1481 808 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2071 2200 2071 Size: 129 6577 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6706 PsiI TTA'TAA Cuts at: 3134 3233 4276 3134 Size: 99 1043 5564 Fragments arranged by size: 5564 1043 99 PstI C_TGCA'G Cuts at: 830 2154 2191 4058 830 Size: 1324 37 1867 3478 Fragments arranged by size: 3478 1867 1324 37 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6706 SalI G'TCGA_C Cuts at: 1936 4048 1936 Size: 2112 4594 SanDI GG'GwC_CC Cuts at: 2200 2200 Size: 6706 SbfI CC_TGCA'GG Cuts at: 4058 4058 Size: 6706 ScaI AGT'ACT Cuts at: 2680 3733 4954 6666 2680 Size: 1053 1221 1712 2720 Fragments arranged by size: 2720 1712 1221 1053 SfcI C'TryA_G Cuts at: 826 1045 2150 2187 2493 2949 3120 3960 4054 Size: 219 1105 37 306 456 171 840 94 Cuts at: 4054 5189 5870 6061 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1105 840 681 456 306 219 191 171 94 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6706 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6706 SmaI CCC'GGG Cuts at: 1872 4033 1872 Size: 2161 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2307 2621 2883 3626 4810 5711 Size: 197 926 364 314 262 743 1184 901 Cuts at: 5711 5973 820 Size: 262 1553 Fragments arranged by size: 1553 1184 926 901 743 364 314 262 262 197 SnaBI TAC'GTA Cuts at: 493 4497 493 Size: 4004 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6706 SphI G_CATG'C Cuts at: 1132 2018 4064 1132 Size: 886 2046 3774 Fragments arranged by size: 3774 2046 886 SspI AAT'ATT Cuts at: 5 52 4630 5 Size: 47 4578 2081 Fragments arranged by size: 4578 2081 47 StuI AGG'CCT Cuts at: 1517 3624 1517 Size: 2107 4599 StyI C'CwwG_G Cuts at: 513 1065 4145 513 Size: 552 3080 3074 Fragments arranged by size: 3080 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3416 3585 4733 4750 4903 5088 5533 Size: 1255 1836 169 1148 17 153 185 445 Cuts at: 5533 325 Size: 1498 Fragments arranged by size: 1836 1498 1255 1148 445 185 169 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2678 3052 3731 Size: 274 80 33 51 1443 701 374 679 Cuts at: 3731 3821 4952 6664 96 Size: 90 1131 1712 138 Fragments arranged by size: 1712 1443 1131 701 679 374 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3980 4012 6666 6698 3980 Size: 32 2654 32 3988 Fragments arranged by size: 3988 2654 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6706 XbaI T'CTAG_A Cuts at: 3796 4042 3796 Size: 246 6460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2440 3410 2440 Size: 970 5736 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6706 XmaI C'CCGG_G Cuts at: 1870 4031 1870 Size: 2161 4545 XmnI GAAnn'nnTTC Cuts at: 4835 4835 Size: 6706 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4514 276 Size: 53 83 186 936 2980 2468 Fragments arranged by size: 2980 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI