Restriction map for
pFN21AA0424
(Linear) (Six-base) MAPSORT of: KIAA0424.seq Check: 7789 from: 1 to: 1548
KIAA0424.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AatII G_ACGT'C
Cuts at: 0 101 1548
Size: 101 1447
AccI GT'mk_AC
Cuts at: 0 775 1548
Size: 775 773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 454 1506 1548
Size: 409 45 1052 42
Fragments arranged by size:
1052 409 45 42
AflIII A'CryG_T
Cuts at: 0 413 1548
Size: 413 1135
AlwNI CAG_nnn'CTG
Cuts at: 0 1440 1548
Size: 1440 108
ApaI G_GGCC'C
Cuts at: 0 227 1548
Size: 227 1321
ApoI r'AATT_y
Cuts at: 0 1307 1548
Size: 1307 241
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1195 1228 1548
Size: 1195 33 320
Fragments arranged by size:
1195 320 33
BanII G_rGCy'C
Cuts at: 0 227 623 767 949 1548
Size: 227 396 144 182 599
Fragments arranged by size:
599 396 227 182 144
BbsI GAAGACnn'nnnn_
Cuts at: 0 1008 1548
Size: 1008 540
BbvCI CC'TCA_GC
Cuts at: 0 38 529 1548
Size: 38 491 1019
Fragments arranged by size:
1019 491 38
BciVI GTATCCnnnnn_n'
Cuts at: 0 755 1548
Size: 755 793
BclI T'GATC_A
Cuts at: 0 11 1548
Size: 11 1537
BglII A'GATC_T
Cuts at: 0 734 1548
Size: 734 814
BlpI GC'TnA_GC
Cuts at: 0 761 1548
Size: 761 787
Bme1580I G_kGCm'C
Cuts at: 0 227 1548
Size: 227 1321
BmgBI CAC'GTC
Cuts at: 0 57 1548
Size: 57 1491
BmrI ACTGGGnnnn_n'
Cuts at: 0 927 974 1548
Size: 927 47 574
Fragments arranged by size:
927 574 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 517 707 1249 1548
Size: 38 479 190 542 299
Fragments arranged by size:
542 479 299 190 38
Bpu10I CC'TnA_GC
Cuts at: 0 38 529 1249 1358 1548
Size: 38 491 720 109 190
Fragments arranged by size:
720 491 190 109 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1548
Size: 349 1199
BsaI GGTCTCn'nnnn_
Cuts at: 0 485 1548
Size: 485 1063
BsaHI Gr'CG_yC
Cuts at: 0 98 1548
Size: 98 1450
BsaWI w'CCGG_w
Cuts at: 0 1064 1548
Size: 1064 484
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 151 181 490 520 1548
Size: 151 30 309 30 1028
Fragments arranged by size:
1028 309 151 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 232 1213 1548
Size: 172 60 981 335
Fragments arranged by size:
981 335 172 60
BseYI C'CCAG_C
Cuts at: 0 226 1548
Size: 226 1322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 748 1548
Size: 256 492 800
Fragments arranged by size:
800 492 256
BsiEI CG_ry'CG
Cuts at: 0 999 1548
Size: 999 549
BsiHKAI G_wGCw'C
Cuts at: 0 340 560 623 767 949 1548
Size: 340 220 63 144 182 599
Fragments arranged by size:
599 340 220 182 144 63
BsmI GAATG_Cn'
Cuts at: 0 1173 1548
Size: 1173 375
Bsp1286I G_dGCh'C
Cuts at: 0 227 340 560 623 767 949 1548
Size: 227 113 220 63 144 182 599
Fragments arranged by size:
599 227 220 182 144 113 63
BspEI T'CCGG_A
Cuts at: 0 1064 1548
Size: 1064 484
BspHI T'CATG_A
Cuts at: 0 827 1548
Size: 827 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1458 1517 1548
Size: 1458 59 31
Fragments arranged by size:
1458 59 31
BsrBI CCG'CTC
Cuts at: 0 1385 1548
Size: 1385 163
BsrDI GCAATG_nn'
Cuts at: 0 695 1343 1548
Size: 695 648 205
Fragments arranged by size:
695 648 205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1227 1548
Size: 1227 321
BstYI r'GATC_y
Cuts at: 0 734 980 1548
Size: 734 246 568
Fragments arranged by size:
734 568 246
BstZ17I GTA'TAC
Cuts at: 0 776 1548
Size: 776 772
BtgI C'CryG_G
Cuts at: 0 62 1428 1548
Size: 62 1366 120
Fragments arranged by size:
1366 120 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 245 1440 1548
Size: 245 1195 108
Fragments arranged by size:
1195 245 108
BtsI GCAGTG_nn'
Cuts at: 0 400 1548
Size: 400 1148
ClaI AT'CG_AT
Cuts at: 0 704 1548
Size: 704 844
DraI TTT'AAA
Cuts at: 0 87 1548
Size: 87 1461
DrdI GACnn_nn'nnGTC
Cuts at: 0 1153 1548
Size: 1153 395
EaeI y'GGCC_r
Cuts at: 0 65 283 996 1431 1548
Size: 65 218 713 435 117
Fragments arranged by size:
713 435 218 117 65
EagI C'GGCC_G
Cuts at: 0 996 1548
Size: 996 552
EarI CTCTTCn'nnn_
Cuts at: 0 397 1259 1318 1548
Size: 397 862 59 230
Fragments arranged by size:
862 397 230 59
EcoICRI GAG'CTC
Cuts at: 0 621 765 947 1548
Size: 621 144 182 601
Fragments arranged by size:
621 601 182 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 409 454 517 707 1249 1506 1548
Size: 38 371 45 63 190 542 257 42
Fragments arranged by size:
542 371 257 190 63 45 42 38
EcoO109I rG'GnC_Cy
Cuts at: 0 223 541 1056 1416 1548
Size: 223 318 515 360 132
Fragments arranged by size:
515 360 318 223 132
EcoRV GAT'ATC
Cuts at: 0 462 1548
Size: 462 1086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 459 765 1188 1548
Size: 459 306 423 360
Fragments arranged by size:
459 423 360 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 11 43 931 963 1548
Size: 11 32 888 32 585
Fragments arranged by size:
888 585 32 32 11
HincII GTy'rAC
Cuts at: 0 1122 1374 1532 1548
Size: 1122 252 158 16
Fragments arranged by size:
1122 252 158 16
HindIII A'AGCT_T
Cuts at: 0 1177 1548
Size: 1177 371
HpaI GTT'AAC
Cuts at: 0 1532 1548
Size: 1532 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1548
Size: 146 1402
MscI TGG'CCA
Cuts at: 0 67 1548
Size: 67 1481
MslI CAynn'nnrTG
Cuts at: 0 563 605 1099 1548
Size: 563 42 494 449
Fragments arranged by size:
563 494 449 42
MspA1I CmG'CkG
Cuts at: 0 814 1011 1548
Size: 814 197 537
Fragments arranged by size:
814 537 197
NcoI C'CATG_G
Cuts at: 0 62 1548
Size: 62 1486
NspI r_CATG'y
Cuts at: 0 417 1548
Size: 417 1131
PciI A'CATG_T
Cuts at: 0 413 1548
Size: 413 1135
PflMI CCAn_nnn'nTGG
Cuts at: 0 1038 1440 1548
Size: 1038 402 108
Fragments arranged by size:
1038 402 108
PpuMI rG'GwC_Cy
Cuts at: 0 541 1056 1416 1548
Size: 541 515 360 132
Fragments arranged by size:
541 515 360 132
PspOMI G'GGCC_C
Cuts at: 0 223 1548
Size: 223 1325
PvuII CAG'CTG
Cuts at: 0 814 1548
Size: 814 734
SacI G_AGCT'C
Cuts at: 0 623 767 949 1548
Size: 623 144 182 599
Fragments arranged by size:
623 599 182 144
SexAI A'CCwGG_T
Cuts at: 0 1439 1548
Size: 1439 109
SfcI C'TryA_G
Cuts at: 0 289 465 798 1548
Size: 289 176 333 750
Fragments arranged by size:
750 333 289 176
SmlI C'TyrA_G
Cuts at: 0 364 1548
Size: 364 1184
SspI AAT'ATT
Cuts at: 0 879 1548
Size: 879 669
StuI AGG'CCT
Cuts at: 0 667 1548
Size: 667 881
StyI C'CwwG_G
Cuts at: 0 62 1548
Size: 62 1486
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 1548
Size: 183 1365
TatI w'GTAC_w
Cuts at: 0 1079 1548
Size: 1079 469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 121 153 1548
Size: 121 32 1395
Fragments arranged by size:
1395 121 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 1548
Size: 75 1473
ZraI GAC'GTC
Cuts at: 0 99 1548
Size: 99 1449
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApaI ApoI BaeI
BanII BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HincII
HindIII HpaI MmeI MscI MslI MspA1I NcoI NspI
PciI PflMI PpuMI PspOMI PvuII SacI SexAI SfcI
SmlI SspI StuI StyI TaqII TatI TstI XcmI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BanI BbeI BcgI BfrBI BglI BmtI BplI
BsaAI BsaBI BsiWI BsmBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII Bsu36I CspCI DraIII EciI EcoNI
EcoRI FseI FspI FspAI HaeII KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XhoI XmaI XmnI