Restriction map for
pFN21AA0418
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbrPI BciVI
BclI BfrI BglII BlnI BsiEI
BsiWI Bsp1407I BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I DraI DraIII
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FspI HindIII
HpaI KpnI MfeI MluI MseI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacI SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 308 TCCGGA T^CCGGA
AcsI 793 GAATTC [GA]^AATT[CT]
ApoI 793 GAATTC [GA]^AATT[CT]
Asp700I 2772 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 123 GAGCAC G[AT]GC[AT]^C
BalI 647 TGGCCA TGG^CCA
BbsI 1242 GTCTTC GAAGAC(2/6)
BcgI 2520 CGAACGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1865 CTAG C^TAG
BpuAI 1242 GTCTTC GAAGAC(2/6)
BsaBI 1737 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 308 TCCGGA [AT]^CCGG[AT]
BseAI 308 TCCGGA T^CCGGA
BsiHKAI 123 GAGCAC G[AT]GC[AT]^C
BsmBI 2652 CGTCTC CGTCTC(1/5)
BspDI 925 ATCGAT AT^CGAT
BspEI 308 TCCGGA T^CCGGA
BstYI 2756 AGATCC [GA]^GATC[CT]
ClaI 925 ATCGAT AT^CGAT
CpoI 1685 CGGTCCG CG^G[AT]CCG
DrdI 607 GACAGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 793 GAATTC G^AATTC
FseI 423 GGCCGGCC GGCCGG^CC
HincII 2236 GTCAAC GT[CT]^[GA]AC
HindII 2236 GTCAAC GT[CT]^[GA]AC
MaeI 1865 CTAG C^TAG
MamI 1737 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2756 AGATCC [GA]^GATC[CT]
MluNI 647 TGGCCA TGG^CCA
MroI 308 TCCGGA T^CCGGA
MscI 647 TGGCCA TGG^CCA
MslI 2577 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2711 CCATGG C^CATGG
NdeI 1301 CATATG CA^TATG
NspI 1960 ACATGC [GA]CATG^[CT]
PpuMI 146 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 1213 CTGCAG CTGCA^G
RsrII 1685 CGGTCCG CG^G[AT]CCG
ScaI 776 AGTACT AGT^ACT
SfcI 1213 CTGCAG C^T[GA][CT]AG
Tsp509I 794 AATT ^AATT
XhoII 2756 AGATCC [GA]^GATC[CT]
XmnI 2772 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 1312 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1659 GACCTCCTGTC
AocI 1160 CCTCAGG CC^T[ACGT]AGG
2300 CCTCAGG
AspEI 1312 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1659 GACCTCCTGTC
BbeI 363 GGCGCC GGCGC^C
1757 GGCGCC
BbvCI 661 CCTCAGC CCTCAGC(-5/-2)
1478 CCTCAGC
BlpI 57 GCTGAGC GC^T[ACGT]AGC
1131 GCTGAGC
Bpu1102I 57 GCTGAGC GC^T[ACGT]AGC
1131 GCTGAGC
BsaAI 16 TACGTG [CT]AC^GT[GA]
880 TACGTG
BsaI 1270 GAGACC GGTCTC(1/5)
1767 GAGACC
BsmI 1446 GAATGC GAATGC(1/-1)
1105 GCATTC
BstXI 155 CCACCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1779 CCAGCTCCGTGG
Bsu36I 1160 CCTCAGG CC^T[ACGT]AGG
2300 CCTCAGG
CelII 57 GCTGAGC GC^T[ACGT]AGC
1131 GCTGAGC
Eam1105I 1312 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1659 GACCTCCTGTC
Eco81I 1160 CCTCAGG CC^T[ACGT]AGG
2300 CCTCAGG
EcoNI 378 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1260 CCTGGAAGAGG
KasI 363 GGCGCC G^GCGCC
1757 GGCGCC
NaeI 424 GCCGGC GCC^GGC
1200 GCCGGC
NarI 363 GGCGCC GG^CGCC
1757 GGCGCC
NgoMIV 424 GCCGGC G^CCGGC
1200 GCCGGC
PflMI 2094 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2754 CCAGATCCTGG
PvuII 641 CAGCTG CAG^CTG
1762 CAGCTG
SfoI 363 GGCGCC GGC^GCC
1757 GGCGCC
SmaI 1670 CCCGGG CCC^GGG
2281 CCCGGG
Van91I 2094 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2754 CCAGATCCTGG
XmaCI 1670 CCCGGG C^CCGGG
2281 CCCGGG
XmaI 1670 CCCGGG C^CCGGG
2281 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI