Restriction map for
pFN21AA0418
(Linear) (Six-base) MAPSORT of: KIAA0418.seq Check: 1308 from: 1 to: 2820
KIAA0418.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2013 2331 2820
Size: 2013 318 489
Fragments arranged by size:
2013 489 318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1229 1594 2820
Size: 400 829 365 1226
Fragments arranged by size:
1226 829 400 365
AhdI GACnn_n'nnGTC
Cuts at: 0 1317 1664 2820
Size: 1317 347 1156
Fragments arranged by size:
1317 1156 347
AlwNI CAG_nnn'CTG
Cuts at: 0 1126 2028 2760 2820
Size: 1126 902 732 60
Fragments arranged by size:
1126 902 732 60
ApaI G_GGCC'C
Cuts at: 0 672 912 1033 1224 2088 2820
Size: 672 240 121 191 864 732
Fragments arranged by size:
864 732 672 240 191 121
ApoI r'AATT_y
Cuts at: 0 793 2820
Size: 793 2027
AvaI C'yCGr_G
Cuts at: 0 182 1670 2281 2820
Size: 182 1488 611 539
Fragments arranged by size:
1488 611 539 182
BanI G'GyrC_C
Cuts at: 0 363 984 1757 2790 2820
Size: 363 621 773 1033 30
Fragments arranged by size:
1033 773 621 363 30
BanII G_rGCy'C
Cuts at: 0 637 672 758 912 1033 1145 1224 1796
Size: 637 35 86 154 121 112 79 572
Cuts at: 1796 1804 2088 2820
Size: 8 284 732
Fragments arranged by size:
732 637 572 284 154 121 112 86
79 35 8
BbeI G_GCGC'C
Cuts at: 0 367 1761 2820
Size: 367 1394 1059
Fragments arranged by size:
1394 1059 367
BbsI GAAGACnn'nnnn_
Cuts at: 0 1235 2820
Size: 1235 1585
BbvCI CC'TCA_GC
Cuts at: 0 662 1479 2820
Size: 662 817 1341
Fragments arranged by size:
1341 817 662
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2509 2543 2820
Size: 2509 34 277
Fragments arranged by size:
2509 277 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1191 1846 2450 2500 2524 2820
Size: 1191 655 604 50 24 296
Fragments arranged by size:
1191 655 604 296 50 24
BlpI GC'TnA_GC
Cuts at: 0 58 1132 2820
Size: 58 1074 1688
Fragments arranged by size:
1688 1074 58
Bme1580I G_kGCm'C
Cuts at: 0 672 912 989 1033 1224 2088 2159 2820
Size: 672 240 77 44 191 864 71 661
Fragments arranged by size:
864 672 661 240 191 77 71 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 424 1847 2352 2820
Size: 424 1423 505 468
Fragments arranged by size:
1423 505 468 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 184 236 603 623 2050 2095 2218
Size: 49 135 52 367 20 1427 45 123
Cuts at: 2218 2294 2743 2820
Size: 76 449 77
Fragments arranged by size:
1427 449 367 135 123 77 76 52
49 45 20
Bpu10I CC'TnA_GC
Cuts at: 0 662 949 1127 1479 2820
Size: 662 287 178 352 1341
Fragments arranged by size:
1341 662 352 287 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1417 1780 2820
Size: 1057 360 363 1040
Fragments arranged by size:
1057 1040 363 360
BsaI GGTCTCn'nnnn_
Cuts at: 0 1264 1761 2820
Size: 1264 497 1059
Fragments arranged by size:
1264 1059 497
BsaAI yAC'GTr
Cuts at: 0 18 882 2820
Size: 18 864 1938
Fragments arranged by size:
1938 864 18
BsaBI GATnn'nnATC
Cuts at: 0 1741 2820
Size: 1741 1079
BsaHI Gr'CG_yC
Cuts at: 0 290 364 484 1758 2453 2820
Size: 290 74 120 1274 695 367
Fragments arranged by size:
1274 695 367 290 120 74
BsaWI w'CCGG_w
Cuts at: 0 308 2820
Size: 308 2512
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 687 717 1075 1105 1333 1363 2032 2062
Size: 687 30 358 30 228 30 669 30
Cuts at: 2062 2692 2722 2820
Size: 630 30 98
Fragments arranged by size:
687 669 630 358 228 98 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 238 1039 1096 1153 1282 1323 1353
Size: 148 90 801 57 57 129 41 30
Cuts at: 1353 1467 1515 1518 1527 1530 1533 2457 2695
Size: 114 48 3 9 3 3 924 238
Cuts at: 2695 2820
Size: 125
Fragments arranged by size:
924 801 238 148 129 125 114 90
57 57 48 41 30 9 3 3
3
BseYI C'CCAG_C
Cuts at: 0 143 359 905 1003 1181 1651 1896 2081
Size: 143 216 546 98 178 470 245 185
Cuts at: 2081 2564 2786 2820
Size: 483 222 34
Fragments arranged by size:
546 483 470 245 222 216 185 178
143 98 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 904 2041 2820
Size: 262 642 1137 779
Fragments arranged by size:
1137 779 642 262
BsiHKAI G_wGCw'C
Cuts at: 0 127 2820
Size: 127 2693
BsmI GAATG_Cn'
Cuts at: 0 1105 1452 2820
Size: 1105 347 1368
Fragments arranged by size:
1368 1105 347
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2658 2820
Size: 2658 162
Bsp1286I G_dGCh'C
Cuts at: 0 127 637 672 758 912 989 1033 1145
Size: 127 510 35 86 154 77 44 112
Cuts at: 1145 1224 1796 1804 2088 2159 2820
Size: 79 572 8 284 71 661
Fragments arranged by size:
661 572 510 284 154 127 112 86
79 77 71 44 35 8
BspEI T'CCGG_A
Cuts at: 0 308 2820
Size: 308 2512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 407 476 2013 2331 2820
Size: 407 69 1537 318 489
Fragments arranged by size:
1537 489 407 318 69
BsrBI CCG'CTC
Cuts at: 0 107 1583 2045 2820
Size: 107 1476 462 775
Fragments arranged by size:
1476 775 462 107
BsrDI GCAATG_nn'
Cuts at: 0 536 579 785 2820
Size: 536 43 206 2035
Fragments arranged by size:
2035 536 206 43
BsrFI r'CCGG_y
Cuts at: 0 424 1190 1200 2820
Size: 424 766 10 1620
Fragments arranged by size:
1620 766 424 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 162 1786 2820
Size: 162 1624 1034
Fragments arranged by size:
1624 1034 162
BstYI r'GATC_y
Cuts at: 0 2756 2820
Size: 2756 64
Bsu36I CC'TnA_GG
Cuts at: 0 1161 2301 2820
Size: 1161 1140 519
Fragments arranged by size:
1161 1140 519
BtgI C'CryG_G
Cuts at: 0 593 1063 1145 1605 1785 2006 2711 2820
Size: 593 470 82 460 180 221 705 109
Fragments arranged by size:
705 593 470 460 221 180 109 82
BtsI GCAGTG_nn'
Cuts at: 0 594 2477 2820
Size: 594 1883 343
Fragments arranged by size:
1883 594 343
ClaI AT'CG_AT
Cuts at: 0 926 2820
Size: 926 1894
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1545 1580 2820
Size: 1545 35 1240
Fragments arranged by size:
1545 1240 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 613 2820
Size: 613 2207
EaeI y'GGCC_r
Cuts at: 0 422 647 1297 2432 2516 2820
Size: 422 225 650 1135 84 304
Fragments arranged by size:
1135 650 422 304 225 84
EarI CTCTTCn'nnn_
Cuts at: 0 215 1212 1259 1536 1551 2820
Size: 215 997 47 277 15 1269
Fragments arranged by size:
1269 997 277 215 47 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 184 236 400 603 623 1229 1594
Size: 49 135 52 164 203 20 606 365
Cuts at: 1594 2050 2095 2218 2294 2743 2820
Size: 456 45 123 76 449 77
Fragments arranged by size:
606 456 449 365 203 164 135 123
77 76 52 49 45 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 382 1264 2820
Size: 382 882 1556
Fragments arranged by size:
1556 882 382
EcoO109I rG'GnC_Cy
Cuts at: 0 147 531 909 1029 1030 2085 2354 2820
Size: 147 384 378 120 1 1055 269 466
Fragments arranged by size:
1055 466 384 378 269 147 120 1
EcoRI G'AATT_C
Cuts at: 0 793 2820
Size: 793 2027
FseI GG_CCGG'CC
Cuts at: 0 428 2820
Size: 428 2392
HaeII r_GCGC'y
Cuts at: 0 367 1761 1970 2820
Size: 367 1394 209 850
Fragments arranged by size:
1394 850 367 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 645 677 687 719 847 879 1144 1176
Size: 645 32 10 32 128 32 265 32
Cuts at: 1176 1303 1333 1335 1365 1650 1682 2692 2724
Size: 127 30 2 30 285 32 1010 32
Cuts at: 2724 2820
Size: 96
Fragments arranged by size:
1010 645 285 265 128 127 96 32
32 32 32 32 32 30 30 10
2
HincII GTy'rAC
Cuts at: 0 2238 2820
Size: 2238 582
KasI G'GCGC_C
Cuts at: 0 363 1757 2820
Size: 363 1394 1063
Fragments arranged by size:
1394 1063 363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 218 759 1904 2481 2820
Size: 53 165 541 1145 577 339
Fragments arranged by size:
1145 577 541 339 165 53
MscI TGG'CCA
Cuts at: 0 649 2820
Size: 649 2171
MslI CAynn'nnrTG
Cuts at: 0 2581 2820
Size: 2581 239
MspA1I CmG'CkG
Cuts at: 0 643 666 1065 1218 1607 1764 2008 2314
Size: 643 23 399 153 389 157 244 306
Cuts at: 2314 2491 2820
Size: 177 329
Fragments arranged by size:
643 399 389 329 306 244 177 157
153 23
NaeI GCC'GGC
Cuts at: 0 426 1202 2820
Size: 426 776 1618
Fragments arranged by size:
1618 776 426
NarI GG'CG_CC
Cuts at: 0 364 1758 2820
Size: 364 1394 1062
Fragments arranged by size:
1394 1062 364
NcoI C'CATG_G
Cuts at: 0 2711 2820
Size: 2711 109
NdeI CA'TA_TG
Cuts at: 0 1302 2820
Size: 1302 1518
NgoMIV G'CCGG_C
Cuts at: 0 424 1200 2820
Size: 424 776 1620
Fragments arranged by size:
1620 776 424
NspI r_CATG'y
Cuts at: 0 1964 2820
Size: 1964 856
PasI CC'CwG_GG
Cuts at: 0 630 2820
Size: 630 2190
PflMI CCAn_nnn'nTGG
Cuts at: 0 2100 2760 2820
Size: 2100 660 60
Fragments arranged by size:
2100 660 60
PfoI T'CCnGG_A
Cuts at: 0 5 1982 2696 2759 2820
Size: 5 1977 714 63 61
Fragments arranged by size:
1977 714 63 61 5
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1462 1494 2820
Size: 1462 32 1326
Fragments arranged by size:
1462 1326 32
PpuMI rG'GwC_Cy
Cuts at: 0 147 2820
Size: 147 2673
PspOMI G'GGCC_C
Cuts at: 0 668 908 1029 1220 2084 2820
Size: 668 240 121 191 864 736
Fragments arranged by size:
864 736 668 240 191 121
PstI C_TGCA'G
Cuts at: 0 1217 2820
Size: 1217 1603
PvuII CAG'CTG
Cuts at: 0 643 1764 2820
Size: 643 1121 1056
Fragments arranged by size:
1121 1056 643
RsrII CG'GwC_CG
Cuts at: 0 1686 2820
Size: 1686 1134
SacII CC_GC'GG
Cuts at: 0 1066 1608 2009 2820
Size: 1066 542 401 811
Fragments arranged by size:
1066 811 542 401
SanDI GG'GwC_CC
Cuts at: 0 147 2820
Size: 147 2673
ScaI AGT'ACT
Cuts at: 0 778 2820
Size: 778 2042
SfcI C'TryA_G
Cuts at: 0 1213 2820
Size: 1213 1607
SfoI GGC'GCC
Cuts at: 0 365 1759 2820
Size: 365 1394 1061
Fragments arranged by size:
1394 1061 365
SmaI CCC'GGG
Cuts at: 0 1672 2283 2820
Size: 1672 611 537
Fragments arranged by size:
1672 611 537
SmlI C'TyrA_G
Cuts at: 0 1072 1432 1795 2806 2820
Size: 1072 360 363 1011 14
Fragments arranged by size:
1072 1011 363 360 14
StyI C'CwwG_G
Cuts at: 0 389 980 1013 1616 2162 2384 2504 2711
Size: 389 591 33 603 546 222 120 207
Cuts at: 2711 2820
Size: 109
Fragments arranged by size:
603 591 546 389 222 207 120 109
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1225 1674 2397 2778 2820
Size: 1225 449 723 381 42
Fragments arranged by size:
1225 723 449 381 42
TatI w'GTAC_w
Cuts at: 0 776 2820
Size: 776 2044
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 2032 2064 2820
Size: 313 32 1687 32 756
Fragments arranged by size:
1687 756 313 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2511 2718 2761 2820
Size: 2511 207 43 59
Fragments arranged by size:
2511 207 59 43
XmaI C'CCGG_G
Cuts at: 0 1670 2281 2820
Size: 1670 611 539
Fragments arranged by size:
1670 611 539
XmnI GAAnn'nnTTC
Cuts at: 0 2776 2820
Size: 2776 44
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstXI BstYI
Bsu36I BtgI BtsI ClaI CspCI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FseI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII RsrII SacII SanDI ScaI SfcI
SfoI SmaI SmlI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BsiEI BsiWI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI DraI DraIII EagI EciI
EcoICRI EcoRV FalI FspI FspAI HindIII HpaI KpnI
MfeI MluI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI SacI
SalI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI ZraI