Restriction map for
pFN21AA0417
##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflIII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BbrPI BbvCI BcgI
BclI BfaI BglII BlnI BsaI
BseAI BseRI BsiWI Bsp1407I BspEI
BspLU11I BspMI BsrGI BssHII Bst1107I
BstXI BstZ17I CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV FbaI FseI FspI
HgaI HindIII HpaI KpnI MaeI
MfeI MluI MluNI MroI MscI
MunI NdeI NheI NotI NruI
NspI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SapI SbfI
SexAI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI TfiI
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 1696 GACGTC GACGT^C
AflII 1003 CTTAAG C^TTAAG
Alw44I 1689 GTGCAC G^TGCAC
AocI 715 CCTGAGG CC^T[ACGT]AGG
ApaI 31 GGGCCC GGGCC^C
ApaLI 1689 GTGCAC G^TGCAC
Asp700I 1066 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1822 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 375 GGATCC G^GATCC
BanII 31 GGGCCC G[GA]GC[CT]^C
BbeI 1458 GGCGCC GGCGC^C
BbsI 291 GAAGAC GAAGAC(2/6)
BciVI 453 GGATAC GTATCC(6/5)
BfrI 1003 CTTAAG C^TTAAG
BglI 655 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 768 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 768 GCTGAGC GC^T[ACGT]AGC
BpuAI 291 GAAGAC GAAGAC(2/6)
BsaAI 1633 TACGTG [CT]AC^GT[GA]
BsaBI 1509 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1594 CGGTCG CG[GA][CT]^CG
BsmBI 144 CGTCTC CGTCTC(1/5)
BsmI 235 GAATGC GAATGC(1/-1)
BspDI 1366 ATCGAT AT^CGAT
BspHI 641 TCATGA T^CATGA
BsrBI 1600 CCGCTC CCGCTC(-3/-3)
BssSI 166 CTCGTG CACGAG(-5/-1)
Bsu36I 715 CCTGAGG CC^T[ACGT]AGG
CelII 768 GCTGAGC GC^T[ACGT]AGC
ClaI 1366 ATCGAT AT^CGAT
EarI 1989 GAAGAG CTCTTC(1/4)
Eco81I 715 CCTGAGG CC^T[ACGT]AGG
EcoT22I 237 ATGCAT ATGCA^T
KasI 1458 GGCGCC G^GCGCC
Ksp632I 1989 GAAGAG CTCTTC(1/4)
MamI 1509 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1458 GGCGCC GG^CGCC
NcoI 449 CCATGG C^CATGG
NsiI 237 ATGCAT ATGCA^T
PflFI 1822 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1783 CAGCTG CAG^CTG
RcaI 641 TCATGA T^CATGA
SalI 181 GTCGAC G^TCGAC
ScaI 521 AGTACT AGT^ACT
SfoI 1458 GGCGCC GGC^GCC
SmaI 1846 CCCGGG CCC^GGG
SspI 1083 AATATT AAT^ATT
StuI 666 AGGCCT AGG^CCT
Tth111I 1822 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1846 CCCGGG C^CCGGG
XmaI 1846 CCCGGG C^CCGGG
XmnI 1066 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 181 GTCGAC GT^[AC][GT]AC
961 GTAGAC
AccII 1249 CGCG CG^CG
1575 CGCG
AcyI 1458 GGCGCC G[GA]^CG[CT]C
1696 GACGTC
AlwNI 394 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
535 CAGGCGCTG
BsaHI 1458 GGCGCC G[GA]^CG[CT]C
1696 GACGTC
BsaWI 983 TCCGGT [AT]^CCGG[AT]
990 ACCGGA
BsgI 427 CTGCAC GTGCAG(16/14)
1480 GTGCAG
BsrDI 702 CATTGC GCAATG(2/0)
1283 GCAATG
BstBI 1063 TTCGAA TT^CGAA
1986 TTCGAA
BstEII 268 GGTGACC G^GT[ACGT]ACC
986 GGTTACC
BstPI 268 GGTGACC G^GT[ACGT]ACC
986 GGTTACC
BstUI 1249 CGCG CG^CG
1575 CGCG
DraIII 1255 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1680 CACTCGGTG
EcoO65I 268 GGTGACC G^GT[ACGT]ACC
986 GGTTACC
HaeII 537 GGCGCT [GA]GCGC^[CT]
1458 GGCGCC
Hin1I 1458 GGCGCC G[GA]^CG[CT]C
1696 GACGTC
HincII 181 GTCGAC GT[CT]^[GA]AC
1138 GTTGAC
HindII 181 GTCGAC GT[CT]^[GA]AC
1138 GTTGAC
KspI 1248 CCGCGG CCGC^GG
1574 CCGCGG
MvnI 1249 CGCG CG^CG
1575 CGCG
NaeI 16 GCCGGC GCC^GGC
627 GCCGGC
NgoMIV 16 GCCGGC G^CCGGC
627 GCCGGC
NspV 1063 TTCGAA TT^CGAA
1986 TTCGAA
PpuMI 440 GGGACCT [GA]G^G[AT]CC[CT]
1391 GGGACCT
SacII 1248 CCGCGG CCGC^GG
1574 CCGCGG
SfuI 1063 TTCGAA TT^CGAA
1986 TTCGAA
SmlI 1003 CTTAAG C^T[CT][GA]AG
1543 CTCAAG
Tsp509I 1109 AATT ^AATT
2020 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanI BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XhoII