(Circular) (Six-base) MAPSORT of: pFN21AA0417.seq Check: 2482 from: 1 to: 6730 pFN21AA0417 6730 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3705 4540 278 Size: 53 83 186 936 2169 835 2468 Fragments arranged by size: 2468 2169 936 835 186 83 53 AccI GT'mk_AC Cuts at: 1937 2187 2967 4073 1937 Size: 250 780 1106 4594 Fragments arranged by size: 4594 1106 780 250 Acc65I G'GTAC_C Cuts at: 4051 4051 Size: 6730 AclI AA'CG_TT Cuts at: 1563 4857 5230 1563 Size: 3294 373 3063 Fragments arranged by size: 3294 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2567 4793 6177 6445 1632 Size: 935 2226 1384 268 1917 Fragments arranged by size: 2226 1917 1384 935 268 AflII C'TTAA_G Cuts at: 820 1017 3008 820 Size: 197 1991 4542 Fragments arranged by size: 4542 1991 197 AflIII A'CryG_T Cuts at: 1203 5629 6606 1203 Size: 4426 977 1327 Fragments arranged by size: 4426 1327 977 AgeI A'CCGG_T Cuts at: 5561 5561 Size: 6730 AhdI GACnn_n'nnGTC Cuts at: 5459 5459 Size: 6730 AleI CACnn'nnGTG Cuts at: 1166 3908 1166 Size: 2742 3988 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3926 1184 Size: 2742 3988 AlwNI CAG_nnn'CTG Cuts at: 2404 2545 4473 6045 2404 Size: 141 1928 1572 3089 Fragments arranged by size: 3089 1928 1572 141 ApaI G_GGCC'C Cuts at: 2040 2040 Size: 6730 ApaLI G'TGCA_C Cuts at: 3694 4787 5943 3694 Size: 1093 1156 4481 Fragments arranged by size: 4481 1156 1093 ApoI r'AATT_y Cuts at: 1493 4039 4265 4417 6526 1493 Size: 2546 226 152 2109 1697 Fragments arranged by size: 2546 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5284 160 Size: 872 4252 1606 Fragments arranged by size: 4252 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2324 2684 3413 3851 4055 1870 Size: 73 97 284 360 729 438 204 4545 Fragments arranged by size: 4545 729 438 360 284 204 97 73 BamHI G'GATC_C Cuts at: 2380 4060 4427 2380 Size: 1680 367 4683 Fragments arranged by size: 4683 1680 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2611 2960 3463 3554 3910 Size: 325 217 627 824 349 503 91 356 Cuts at: 3910 4051 5506 618 Size: 141 1455 1842 Fragments arranged by size: 1842 1455 824 627 503 356 349 325 217 141 91 BanII G_rGCy'C Cuts at: 1391 2040 4049 1391 Size: 649 2009 4072 Fragments arranged by size: 4072 2009 649 BbeI G_GCGC'C Cuts at: 3467 3467 Size: 6730 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2303 928 Size: 971 404 5355 Fragments arranged by size: 5355 971 404 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4921 4955 1903 Size: 34 2984 34 3678 Fragments arranged by size: 3678 2984 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2452 4622 5832 2452 Size: 2170 1210 3350 Fragments arranged by size: 3350 2170 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6730 BfrBI ATG'CAT Cuts at: 2244 2244 Size: 6730 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2666 5341 136 Size: 107 122 71 631 605 994 2675 1525 Fragments arranged by size: 2675 1525 994 631 605 122 107 71 BglII A'GATC_T Cuts at: 6725 6725 Size: 6730 BlpI GC'TnA_GC Cuts at: 2774 4147 2774 Size: 1373 5357 Bme1580I G_kGCm'C Cuts at: 1790 2040 3261 3698 3915 4791 5947 1790 Size: 250 1221 437 217 876 1156 2573 Fragments arranged by size: 2573 1221 1156 876 437 250 217 BmgBI CAC'GTC Cuts at: 1318 3532 1318 Size: 2214 4516 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2451 4461 5419 Size: 416 53 83 399 24 1034 2010 958 Cuts at: 5419 442 Size: 1753 Fragments arranged by size: 2010 1753 1034 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6730 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2746 2746 Size: 6730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 2432 2939 5390 1239 Size: 819 374 507 2451 2579 Fragments arranged by size: 2579 2451 819 507 374 Bpu10I CC'TnA_GC Cuts at: 2841 2841 Size: 6730 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3533 4855 5720 6018 3533 Size: 1322 865 298 4245 Fragments arranged by size: 4245 1322 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5393 882 Size: 617 3894 2219 Fragments arranged by size: 3894 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3640 4521 493 Size: 711 2436 881 2702 Fragments arranged by size: 2702 2436 881 711 BsaBI GATnn'nnATC Cuts at: 3518 6724 3518 Size: 3206 3524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3464 3702 4537 4919 Size: 53 83 186 936 1931 238 835 382 Cuts at: 4919 5554 275 Size: 635 1451 Fragments arranged by size: 1931 1451 936 835 635 382 238 186 83 53 BsaWI w'CCGG_w Cuts at: 2988 2995 5163 5561 5835 5982 6393 2988 Size: 7 2168 398 274 147 411 3325 Fragments arranged by size: 3325 2168 411 398 274 147 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2126 2156 1178 Size: 30 165 30 723 30 5752 Fragments arranged by size: 5752 723 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6730 BseYI C'CCAG_C Cuts at: 2825 3786 5933 6429 2825 Size: 961 2147 496 3126 Fragments arranged by size: 3126 2147 961 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2417 3506 1163 Size: 573 681 1089 4387 Fragments arranged by size: 4387 1089 681 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3602 4180 4941 5969 1540 Size: 275 186 1601 578 761 1028 2301 Fragments arranged by size: 2301 1601 1028 761 578 275 186 BsiHKAI G_wGCw'C Cuts at: 3273 3698 3825 4791 4876 5947 3273 Size: 425 127 966 85 1071 4056 Fragments arranged by size: 4056 1071 966 425 127 85 BsmI GAATG_Cn' Cuts at: 2246 4241 4334 2246 Size: 1995 93 4642 Fragments arranged by size: 4642 1995 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2155 2155 Size: 6730 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 3261 3273 3698 3825 3915 4049 Size: 399 250 1221 12 425 127 90 134 Cuts at: 4049 4791 4876 5947 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1221 1071 742 425 399 250 134 127 90 85 12 BspEI T'CCGG_A Cuts at: 6393 6393 Size: 6730 BspHI T'CATG_A Cuts at: 2646 4619 6349 2646 Size: 1973 1730 3027 Fragments arranged by size: 3027 1973 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4085 844 Size: 3241 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3607 4617 1394 Size: 429 1784 1010 3507 Fragments arranged by size: 3507 1784 1010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2706 3295 5225 5399 65 Size: 1183 1458 589 1930 174 1396 Fragments arranged by size: 1930 1458 1396 1183 589 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2632 3597 5374 5561 1700 Size: 110 211 611 965 1777 187 2869 Fragments arranged by size: 2869 1777 965 611 211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6730 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6730 BssSI C'ACGA_G Cuts at: 2171 4790 5802 2171 Size: 2619 1012 3099 Fragments arranged by size: 3099 2619 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3277 3322 3484 794 Size: 2483 45 162 4040 Fragments arranged by size: 4040 2483 162 45 BstBI TT'CG_AA Cuts at: 3069 3992 5534 3069 Size: 923 1542 4265 Fragments arranged by size: 4265 1542 923 BstEII G'GTnAC_C Cuts at: 1184 2273 2991 1184 Size: 1089 718 4923 Fragments arranged by size: 4923 1089 718 BstYI r'GATC_y Cuts at: 1972 2380 2985 3044 3883 3927 4060 4427 4812 Size: 408 605 59 839 44 133 367 385 Cuts at: 4812 4829 6281 6367 6379 6725 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 839 605 408 385 367 346 133 86 59 44 17 12 Bsu36I CC'TnA_GG Cuts at: 2721 2721 Size: 6730 BtgI C'CryG_G Cuts at: 513 1065 2004 2454 3253 3579 513 Size: 552 939 450 799 326 3664 Fragments arranged by size: 3664 939 799 552 450 326 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2275 487 Size: 683 56 1049 4942 Fragments arranged by size: 4942 1049 683 56 BtsI GCAGTG_nn' Cuts at: 2405 2815 3903 4246 5040 5060 2405 Size: 410 1088 343 794 20 4075 Fragments arranged by size: 4075 1088 794 410 343 20 ClaI AT'CG_AT Cuts at: 1554 3372 1554 Size: 1818 4912 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3958 3993 529 Size: 35 3394 35 3266 Fragments arranged by size: 3394 3266 35 35 DraI TTT'AAA Cuts at: 4034 4034 Size: 6730 DraIII CAC_nnn'GTG Cuts at: 3265 3690 3265 Size: 425 6305 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2147 2975 3581 5737 809 Size: 507 831 828 606 2156 1802 Fragments arranged by size: 2156 1802 831 828 606 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2634 3774 Size: 54 1425 50 275 18 351 453 1140 Cuts at: 3774 4177 5066 6695 8 Size: 403 889 1629 43 Fragments arranged by size: 1629 1425 1140 889 453 403 351 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4177 1537 Size: 275 2365 4090 Fragments arranged by size: 4090 2365 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3989 4660 1353 Size: 2636 671 3423 Fragments arranged by size: 3423 2636 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3048 3866 5317 5691 5837 Size: 1064 138 435 1024 818 1451 374 146 Cuts at: 5837 387 Size: 1280 Fragments arranged by size: 1451 1280 1064 1024 818 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2432 2567 2939 4793 5390 6177 Size: 393 426 374 135 372 1854 597 787 Cuts at: 6177 6445 1239 Size: 268 1524 Fragments arranged by size: 1854 1524 787 597 426 393 374 372 268 135 EcoO109I rG'GnC_Cy Cuts at: 2037 2446 3397 2037 Size: 409 951 5370 Fragments arranged by size: 5370 951 409 EcoRI G'AATT_C Cuts at: 4039 4417 4039 Size: 378 6352 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3454 3454 Size: 6730 FspI TGC'GCA Cuts at: 5236 5236 Size: 6730 HaeII r_GCGC'y Cuts at: 1678 2546 3467 5877 1678 Size: 868 921 2410 2531 Fragments arranged by size: 2531 2410 921 868 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2126 2158 2589 2621 2965 Size: 32 135 32 554 32 431 32 344 Cuts at: 2965 2997 5371 5403 5445 5477 1373 Size: 32 2374 32 42 32 2626 Fragments arranged by size: 2626 2374 554 431 344 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2188 3145 4074 4320 669 Size: 988 281 250 957 929 246 3079 Fragments arranged by size: 3079 988 957 929 281 250 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6730 HpaI GTT'AAC Cuts at: 4320 4320 Size: 6730 KasI G'GCGC_C Cuts at: 3463 3463 Size: 6730 KpnI G_GTAC'C Cuts at: 4055 4055 Size: 6730 MfeI C'AATT_G Cuts at: 4329 4329 Size: 6730 MluI A'CGCG_T Cuts at: 6606 6606 Size: 6730 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 2327 2852 5844 6028 1305 Size: 864 158 525 2992 184 2007 Fragments arranged by size: 2992 2007 864 525 184 158 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4908 Fragments arranged by size: 4908 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 2609 3590 3908 4688 5047 5206 Size: 648 1083 360 981 318 780 359 159 Cuts at: 5206 518 Size: 2042 Fragments arranged by size: 2042 1083 981 780 648 360 359 318 159 MspA1I CmG'CkG Cuts at: 1606 2805 3205 3255 3581 3790 4146 4823 5971 Size: 1199 400 50 326 209 356 677 1148 Cuts at: 5971 6216 1606 Size: 245 2120 Fragments arranged by size: 2120 1199 1148 677 400 356 326 245 209 50 NaeI GCC'GGC Cuts at: 2023 2634 2023 Size: 611 6119 NarI GG'CG_CC Cuts at: 3464 3464 Size: 6730 NcoI C'CATG_G Cuts at: 513 1065 2004 2454 513 Size: 552 939 450 4789 Fragments arranged by size: 4789 939 552 450 NdeI CA'TA_TG Cuts at: 387 387 Size: 6730 NgoMIV G'CCGG_C Cuts at: 2021 2632 2021 Size: 611 6119 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6730 NotI GC'GGCC_GC Cuts at: 4177 4177 Size: 6730 NsiI A_TGCA'T Cuts at: 2246 2246 Size: 6730 NspI r_CATG'y Cuts at: 1132 1229 4088 5633 1132 Size: 97 2859 1545 2229 Fragments arranged by size: 2859 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6730 PciI A'CATG_T Cuts at: 5629 5629 Size: 6730 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2400 3532 3736 1108 Size: 1269 23 1132 204 4102 Fragments arranged by size: 4102 1269 1132 204 23 PfoI T'CCnGG_A Cuts at: 3393 3393 Size: 6730 PmeI GTTT'AAAC Cuts at: 4034 4034 Size: 6730 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2318 2350 4799 4831 6312 6344 2318 Size: 32 2449 32 1481 32 2704 Fragments arranged by size: 2704 2449 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2446 3397 2446 Size: 951 5779 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6730 PsiI TTA'TAA Cuts at: 4300 4300 Size: 6730 PspOMI G'GGCC_C Cuts at: 2036 2036 Size: 6730 PstI C_TGCA'G Cuts at: 830 2526 3136 3477 4082 830 Size: 1696 610 341 605 3478 Fragments arranged by size: 3478 1696 610 605 341 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6730 PvuII CAG'CTG Cuts at: 3790 3790 Size: 6730 SacII CC_GC'GG Cuts at: 3256 3582 3256 Size: 326 6404 SalI G'TCGA_C Cuts at: 1936 2186 4072 1936 Size: 250 1886 4594 Fragments arranged by size: 4594 1886 250 SbfI CC_TGCA'GG Cuts at: 4082 4082 Size: 6730 ScaI AGT'ACT Cuts at: 2528 4978 6690 2528 Size: 2450 1712 2568 Fragments arranged by size: 2568 2450 1712 SfcI C'TryA_G Cuts at: 826 1045 2218 2522 3132 3473 3811 4078 5213 Size: 219 1173 304 610 341 338 267 1135 Cuts at: 5213 5894 6085 826 Size: 681 191 1471 Fragments arranged by size: 1471 1173 1135 681 610 341 338 304 267 219 191 SfoI GGC'GCC Cuts at: 3465 3465 Size: 6730 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6730 SmaI CCC'GGG Cuts at: 1872 3853 4057 1872 Size: 1981 204 4545 Fragments arranged by size: 4545 1981 204 SmlI C'TyrA_G Cuts at: 820 1017 1943 3008 3548 4834 5735 5997 820 Size: 197 926 1065 540 1286 901 262 1553 Fragments arranged by size: 1553 1286 1065 926 901 540 262 197 SnaBI TAC'GTA Cuts at: 493 4521 493 Size: 4028 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6730 SphI G_CATG'C Cuts at: 1132 4088 1132 Size: 2956 3774 SspI AAT'ATT Cuts at: 5 52 3090 4654 5 Size: 47 3038 1564 2081 Fragments arranged by size: 3038 2081 1564 47 StuI AGG'CCT Cuts at: 1517 2673 1517 Size: 1156 5574 StyI C'CwwG_G Cuts at: 513 1065 2004 2229 2454 2712 4169 513 Size: 552 939 225 225 258 1457 3074 Fragments arranged by size: 3074 1457 939 552 258 225 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3734 4757 4774 4927 5112 5557 325 Size: 1255 2154 1023 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 1023 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2526 4976 6688 Size: 274 80 33 51 1443 549 2450 1712 Cuts at: 6688 96 Size: 138 Fragments arranged by size: 2450 1712 1443 549 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6690 6722 6690 Size: 32 6698 Tth111I GACn'n_nGTC Cuts at: 1316 3830 1316 Size: 2514 4216 XbaI T'CTAG_A Cuts at: 4066 4066 Size: 6730 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6730 XmaI C'CCGG_G Cuts at: 1870 3851 4055 1870 Size: 1981 204 4545 Fragments arranged by size: 4545 1981 204 XmnI GAAnn'nnTTC Cuts at: 3075 4859 3075 Size: 1784 4946 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3703 4538 276 Size: 53 83 186 936 2169 835 2468 Fragments arranged by size: 2468 2169 936 835 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI