Restriction map for
pFN21AA0417
(Linear) (Six-base) MAPSORT of: KIAA0417.seq Check: 8739 from: 1 to: 2025
KIAA0417.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AatII G_ACGT'C
Cuts at: 0 1700 2025
Size: 1700 325
AccI GT'mk_AC
Cuts at: 0 182 962 2025
Size: 182 780 1063
Fragments arranged by size:
1063 780 182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 2025
Size: 562 1463
AflII C'TTAA_G
Cuts at: 0 1003 2025
Size: 1003 1022
AleI CACnn'nnGTG
Cuts at: 0 1903 2025
Size: 1903 122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1921 2025
Size: 1921 104
AlwNI CAG_nnn'CTG
Cuts at: 0 399 540 2025
Size: 399 141 1485
Fragments arranged by size:
1485 399 141
ApaI G_GGCC'C
Cuts at: 0 35 2025
Size: 35 1990
ApaLI G'TGCA_C
Cuts at: 0 1689 2025
Size: 1689 336
AvaI C'yCGr_G
Cuts at: 0 35 319 679 1408 1846 2025
Size: 35 284 360 729 438 179
Fragments arranged by size:
729 438 360 284 179 35
BamHI G'GATC_C
Cuts at: 0 375 2025
Size: 375 1650
BanI G'GyrC_C
Cuts at: 0 606 955 1458 1549 1905 2025
Size: 606 349 503 91 356 120
Fragments arranged by size:
606 503 356 349 120 91
BanII G_rGCy'C
Cuts at: 0 35 2025
Size: 35 1990
BbeI G_GCGC'C
Cuts at: 0 1462 2025
Size: 1462 563
BbsI GAAGACnn'nnnn_
Cuts at: 0 298 2025
Size: 298 1727
BciVI GTATCCnnnnn_n'
Cuts at: 0 447 2025
Size: 447 1578
BfrBI ATG'CAT
Cuts at: 0 239 2025
Size: 239 1786
BglI GCCn_nnn'nGGC
Cuts at: 0 661 2025
Size: 661 1364
BlpI GC'TnA_GC
Cuts at: 0 769 2025
Size: 769 1256
Bme1580I G_kGCm'C
Cuts at: 0 35 1256 1693 1910 2025
Size: 35 1221 437 217 115
Fragments arranged by size:
1221 437 217 115 35
BmgBI CAC'GTC
Cuts at: 0 1527 2025
Size: 1527 498
BmrI ACTGGGnnnn_n'
Cuts at: 0 446 2025
Size: 446 1579
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 741 2025
Size: 741 1284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 427 934 2025
Size: 53 374 507 1091
Fragments arranged by size:
1091 507 374 53
Bpu10I CC'TnA_GC
Cuts at: 0 836 2025
Size: 836 1189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1528 2025
Size: 1528 497
BsaAI yAC'GTr
Cuts at: 0 1635 2025
Size: 1635 390
BsaBI GATnn'nnATC
Cuts at: 0 1513 2025
Size: 1513 512
BsaHI Gr'CG_yC
Cuts at: 0 1459 1697 2025
Size: 1459 238 328
Fragments arranged by size:
1459 328 238
BsaWI w'CCGG_w
Cuts at: 0 983 990 2025
Size: 983 7 1035
Fragments arranged by size:
1035 983 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 121 151 2025
Size: 121 30 1874
Fragments arranged by size:
1874 121 30
BseYI C'CCAG_C
Cuts at: 0 820 1781 2025
Size: 820 961 244
Fragments arranged by size:
961 820 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1501 2025
Size: 412 1089 524
Fragments arranged by size:
1089 524 412
BsiEI CG_ry'CG
Cuts at: 0 1597 2025
Size: 1597 428
BsiHKAI G_wGCw'C
Cuts at: 0 1268 1693 1820 2025
Size: 1268 425 127 205
Fragments arranged by size:
1268 425 205 127
BsmI GAATG_Cn'
Cuts at: 0 241 2025
Size: 241 1784
BsmBI CGTCTCn'nnnn_
Cuts at: 0 150 2025
Size: 150 1875
Bsp1286I G_dGCh'C
Cuts at: 0 35 1256 1268 1693 1820 1910 2025
Size: 35 1221 12 425 127 90 115
Fragments arranged by size:
1221 425 127 115 90 35 12
BspHI T'CATG_A
Cuts at: 0 641 2025
Size: 641 1384
BsrBI CCG'CTC
Cuts at: 0 1602 2025
Size: 1602 423
BsrDI GCAATG_nn'
Cuts at: 0 701 1290 2025
Size: 701 589 735
Fragments arranged by size:
735 701 589
BsrFI r'CCGG_y
Cuts at: 0 16 627 1592 2025
Size: 16 611 965 433
Fragments arranged by size:
965 611 433 16
BssSI C'ACGA_G
Cuts at: 0 166 2025
Size: 166 1859
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1272 1317 1479 2025
Size: 1272 45 162 546
Fragments arranged by size:
1272 546 162 45
BstBI TT'CG_AA
Cuts at: 0 1064 1987 2025
Size: 1064 923 38
Fragments arranged by size:
1064 923 38
BstEII G'GTnAC_C
Cuts at: 0 268 986 2025
Size: 268 718 1039
Fragments arranged by size:
1039 718 268
BstYI r'GATC_y
Cuts at: 0 375 980 1039 1878 1922 2025
Size: 375 605 59 839 44 103
Fragments arranged by size:
839 605 375 103 59 44
Bsu36I CC'TnA_GG
Cuts at: 0 716 2025
Size: 716 1309
BtgI C'CryG_G
Cuts at: 0 449 1248 1574 2025
Size: 449 799 326 451
Fragments arranged by size:
799 451 449 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 270 2025
Size: 270 1755
BtsI GCAGTG_nn'
Cuts at: 0 400 810 1898 2025
Size: 400 410 1088 127
Fragments arranged by size:
1088 410 400 127
ClaI AT'CG_AT
Cuts at: 0 1367 2025
Size: 1367 658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1953 1988 2025
Size: 1953 35 37
Fragments arranged by size:
1953 37 35
DraIII CAC_nnn'GTG
Cuts at: 0 1260 1685 2025
Size: 1260 425 340
Fragments arranged by size:
1260 425 340
DrdI GACnn_nn'nnGTC
Cuts at: 0 142 970 1576 2025
Size: 142 828 606 449
Fragments arranged by size:
828 606 449 142
EaeI y'GGCC_r
Cuts at: 0 176 629 1769 2025
Size: 176 453 1140 256
Fragments arranged by size:
1140 453 256 176
EarI CTCTTCn'nnn_
Cuts at: 0 1984 2025
Size: 1984 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1043 1861 2025
Size: 19 1024 818 164
Fragments arranged by size:
1024 818 164 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 427 562 934 2025
Size: 53 374 135 372 1091
Fragments arranged by size:
1091 374 372 135 53
EcoO109I rG'GnC_Cy
Cuts at: 0 32 441 1392 2025
Size: 32 409 951 633
Fragments arranged by size:
951 633 409 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1449 2025
Size: 1449 576
HaeII r_GCGC'y
Cuts at: 0 541 1462 2025
Size: 541 921 563
Fragments arranged by size:
921 563 541
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 584 616 960 992 2025
Size: 121 32 431 32 344 32 1033
Fragments arranged by size:
1033 431 344 121 32 32 32
HincII GTy'rAC
Cuts at: 0 183 1140 2025
Size: 183 957 885
Fragments arranged by size:
957 885 183
KasI G'GCGC_C
Cuts at: 0 1458 2025
Size: 1458 567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 322 847 2025
Size: 164 158 525 1178
Fragments arranged by size:
1178 525 164 158
MslI CAynn'nnrTG
Cuts at: 0 244 604 1585 1903 2025
Size: 244 360 981 318 122
Fragments arranged by size:
981 360 318 244 122
MspA1I CmG'CkG
Cuts at: 0 800 1200 1250 1576 1785 2025
Size: 800 400 50 326 209 240
Fragments arranged by size:
800 400 326 240 209 50
NaeI GCC'GGC
Cuts at: 0 18 629 2025
Size: 18 611 1396
Fragments arranged by size:
1396 611 18
NarI GG'CG_CC
Cuts at: 0 1459 2025
Size: 1459 566
NcoI C'CATG_G
Cuts at: 0 449 2025
Size: 449 1576
NgoMIV G'CCGG_C
Cuts at: 0 16 627 2025
Size: 16 611 1398
Fragments arranged by size:
1398 611 16
NsiI A_TGCA'T
Cuts at: 0 241 2025
Size: 241 1784
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 395 1527 1731 2025
Size: 372 23 1132 204 294
Fragments arranged by size:
1132 372 294 204 23
PfoI T'CCnGG_A
Cuts at: 0 1388 2025
Size: 1388 637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 2025
Size: 313 32 1680
Fragments arranged by size:
1680 313 32
PpuMI rG'GwC_Cy
Cuts at: 0 441 1392 2025
Size: 441 951 633
Fragments arranged by size:
951 633 441
PspOMI G'GGCC_C
Cuts at: 0 31 2025
Size: 31 1994
PstI C_TGCA'G
Cuts at: 0 521 1131 1472 2025
Size: 521 610 341 553
Fragments arranged by size:
610 553 521 341
PvuII CAG'CTG
Cuts at: 0 1785 2025
Size: 1785 240
SacII CC_GC'GG
Cuts at: 0 1251 1577 2025
Size: 1251 326 448
Fragments arranged by size:
1251 448 326
SalI G'TCGA_C
Cuts at: 0 181 2025
Size: 181 1844
ScaI AGT'ACT
Cuts at: 0 523 2025
Size: 523 1502
SfcI C'TryA_G
Cuts at: 0 213 517 1127 1468 1806 2025
Size: 213 304 610 341 338 219
Fragments arranged by size:
610 341 338 304 219 213
SfoI GGC'GCC
Cuts at: 0 1460 2025
Size: 1460 565
SmaI CCC'GGG
Cuts at: 0 1848 2025
Size: 1848 177
SmlI C'TyrA_G
Cuts at: 0 1003 1543 2025
Size: 1003 540 482
Fragments arranged by size:
1003 540 482
SspI AAT'ATT
Cuts at: 0 1085 2025
Size: 1085 940
StuI AGG'CCT
Cuts at: 0 668 2025
Size: 668 1357
StyI C'CwwG_G
Cuts at: 0 224 449 707 2025
Size: 224 225 258 1318
Fragments arranged by size:
1318 258 225 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1729 2025
Size: 1729 296
TatI w'GTAC_w
Cuts at: 0 521 2025
Size: 521 1504
Tth111I GACn'n_nGTC
Cuts at: 0 1825 2025
Size: 1825 200
XmaI C'CCGG_G
Cuts at: 0 1846 2025
Size: 1846 179
XmnI GAAnn'nnTTC
Cuts at: 0 1070 2025
Size: 1070 955
ZraI GAC'GTC
Cuts at: 0 1698 2025
Size: 1698 327
Enzymes that do cut:
AatII AccI AcuI AflII AleI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BciVI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I HincII
KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacII SalI ScaI SfcI SfoI SmaI SmlI SspI
StuI StyI TaqII TatI Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflIII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BclI BglII BmtI BsaI BseRI BsiWI BspEI BspMI
BsrGI BssHII BstXI BstZ17I DraI EagI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MscI NdeI NheI NotI NruI NspI
PacI PasI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SanDI SapI SbfI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
TstI XbaI XcmI XhoI