(Circular) (Six-base) MAPSORT of: pFN21AA0409.seq Check: 4864 from: 1 to: 6073 pFN21AA0409 6073 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3416 1937 Size: 1479 4594 Acc65I G'GTAC_C Cuts at: 3394 3394 Size: 6073 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2283 2406 3170 3332 4136 5520 5788 1632 Size: 651 123 764 162 804 1384 268 1917 Fragments arranged by size: 1917 1384 804 764 651 268 162 123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 4972 5949 1203 Size: 3769 977 1327 Fragments arranged by size: 3769 1327 977 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 4802 4802 Size: 6073 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6073 AlwNI CAG_nnn'CTG Cuts at: 2446 2740 3816 5388 2446 Size: 294 1076 1572 3131 Fragments arranged by size: 3131 1572 1076 294 ApaI G_GGCC'C Cuts at: 2061 2143 2306 2752 2790 3306 3321 2061 Size: 82 163 446 38 516 15 4813 Fragments arranged by size: 4813 516 446 163 82 38 15 ApaLI G'TGCA_C Cuts at: 4130 5286 4130 Size: 1156 4917 ApoI r'AATT_y Cuts at: 1493 2431 3382 3608 3760 5869 1493 Size: 938 951 226 152 2109 1697 Fragments arranged by size: 2109 1697 951 938 226 152 AseI AT'TA_AT Cuts at: 160 1032 4627 160 Size: 872 3595 1606 Fragments arranged by size: 3595 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 2703 3398 1870 Size: 73 760 695 4545 Fragments arranged by size: 4545 760 695 73 AvrII C'CTAG_G Cuts at: 3023 3023 Size: 6073 BamHI G'GATC_C Cuts at: 3403 3770 3403 Size: 367 5706 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2623 3394 4849 618 Size: 325 217 627 836 771 1455 1842 Fragments arranged by size: 1842 1455 836 771 627 325 217 BanII G_rGCy'C Cuts at: 1391 2061 2108 2143 2306 2725 2752 2790 3306 Size: 670 47 35 163 419 27 38 516 Cuts at: 3306 3321 3392 1391 Size: 15 71 4072 Fragments arranged by size: 4072 670 516 419 163 71 47 38 35 27 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2666 2826 928 Size: 971 767 160 4175 Fragments arranged by size: 4175 971 767 160 BbvCI CC'TCA_GC Cuts at: 2643 3177 2643 Size: 534 5539 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4264 4298 1903 Size: 34 2327 34 3678 Fragments arranged by size: 3678 2327 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2990 3965 5175 2990 Size: 975 1210 3888 Fragments arranged by size: 3888 1210 975 BclI T'GATC_A Cuts at: 1548 1548 Size: 6073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 2147 2729 Size: 107 122 71 631 605 361 114 582 Cuts at: 2729 4684 136 Size: 1955 1525 Fragments arranged by size: 1955 1525 631 605 582 361 122 114 107 71 BglII A'GATC_T Cuts at: 6068 6068 Size: 6073 BlpI GC'TnA_GC Cuts at: 3327 3490 3327 Size: 163 5910 Bme1580I G_kGCm'C Cuts at: 1790 2061 2143 2306 2752 2790 3306 3321 4134 Size: 271 82 163 446 38 516 15 813 Cuts at: 4134 5290 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 813 516 446 271 163 82 38 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2033 3309 3804 Size: 416 53 83 399 24 616 1276 495 Cuts at: 3804 4762 442 Size: 958 1753 Fragments arranged by size: 1753 1276 958 616 495 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3080 4733 1239 Size: 1841 1653 2579 Fragments arranged by size: 2579 1841 1653 Bpu10I CC'TnA_GC Cuts at: 2643 3177 2643 Size: 534 5539 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4198 5063 5361 4198 Size: 865 298 4910 Fragments arranged by size: 4910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3167 4736 882 Size: 617 1668 1569 2219 Fragments arranged by size: 2219 1668 1569 617 BsaAI yAC'GTr Cuts at: 493 1204 3864 493 Size: 711 2660 2702 Fragments arranged by size: 2702 2660 711 BsaBI GATnn'nnATC Cuts at: 6067 6067 Size: 6073 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3880 4262 4897 275 Size: 53 83 186 936 2347 382 635 1451 Fragments arranged by size: 2347 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2982 4506 4904 5178 5325 5736 2982 Size: 1524 398 274 147 411 3319 Fragments arranged by size: 3319 1524 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 3053 3083 1178 Size: 30 165 30 687 30 933 30 4168 Fragments arranged by size: 4168 933 687 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2080 2213 2216 2219 2222 3146 1186 Size: 894 133 3 3 3 924 4113 Fragments arranged by size: 4113 924 894 133 3 3 3 BseYI C'CCAG_C Cuts at: 2163 2174 2439 2638 2789 5276 5772 2163 Size: 11 265 199 151 2487 496 2464 Fragments arranged by size: 2487 2464 496 265 199 151 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2784 1163 Size: 573 1048 4452 Fragments arranged by size: 4452 1048 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3523 4284 5312 1540 Size: 275 186 1522 761 1028 2301 Fragments arranged by size: 2301 1522 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4134 4219 5290 4134 Size: 85 1071 4917 Fragments arranged by size: 4917 1071 85 BsmI GAATG_Cn' Cuts at: 3584 3677 3584 Size: 93 5980 BsmBI CGTCTCn'nnnn_ Cuts at: 2813 2813 Size: 6073 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2108 2143 2306 2725 2752 2790 Size: 399 271 47 35 163 419 27 38 Cuts at: 2790 3306 3321 3392 4134 4219 5290 1391 Size: 516 15 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 516 419 399 271 163 85 71 47 38 35 27 15 BspEI T'CCGG_A Cuts at: 2982 5736 2982 Size: 2754 3319 BspHI T'CATG_A Cuts at: 3962 5692 3962 Size: 1730 4343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3428 844 Size: 2584 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3960 1394 Size: 429 2137 3507 Fragments arranged by size: 3507 2137 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2995 4568 4742 65 Size: 1183 1747 1573 174 1396 Fragments arranged by size: 1747 1573 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2574 4717 4904 1700 Size: 110 764 2143 187 2869 Fragments arranged by size: 2869 2143 764 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 2246 794 Size: 1452 4621 BstBI TT'CG_AA Cuts at: 2010 3205 4877 2010 Size: 1195 1672 3206 Fragments arranged by size: 3206 1672 1195 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6073 BstXI CCAn_nnnn'nTGG Cuts at: 3299 3299 Size: 6073 BstYI r'GATC_y Cuts at: 1972 2908 3403 3770 4155 4172 5624 5710 5722 Size: 936 495 367 385 17 1452 86 12 Cuts at: 5722 6068 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 936 495 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2619 2826 2619 Size: 207 5866 BtgI C'CryG_G Cuts at: 513 1065 2046 2848 513 Size: 552 981 802 3738 Fragments arranged by size: 3738 981 802 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2884 487 Size: 683 56 1658 3676 Fragments arranged by size: 3676 1658 683 56 BtsI GCAGTG_nn' Cuts at: 2320 2380 2570 2800 2879 3589 4383 4403 2320 Size: 60 190 230 79 710 794 20 3990 Fragments arranged by size: 3990 794 710 230 190 79 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6038 DraI TTT'AAA Cuts at: 3377 3377 Size: 6073 DraIII CAC_nnn'GTG Cuts at: 3036 3036 Size: 6073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2668 3026 5080 809 Size: 507 1352 358 2054 1802 Fragments arranged by size: 2054 1802 1352 507 358 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2876 3520 Size: 54 1425 50 275 18 297 749 644 Cuts at: 3520 4409 6038 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 749 644 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2007 2816 4003 1353 Size: 654 809 1187 3423 Fragments arranged by size: 3423 1187 809 654 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2067 2584 4660 5034 5180 387 Size: 1064 138 478 517 2076 374 146 1280 Fragments arranged by size: 2076 1280 1064 517 478 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2406 3080 3170 3332 4136 4733 Size: 393 651 123 674 90 162 804 597 Cuts at: 4733 5520 5788 1239 Size: 787 268 1524 Fragments arranged by size: 1524 804 787 674 651 597 393 268 162 123 90 EcoNI CCTnn'n_nnAGG Cuts at: 3131 3312 3131 Size: 181 5892 EcoO109I rG'GnC_Cy Cuts at: 2140 2302 2468 2635 2647 2748 2786 3256 3303 Size: 162 166 167 12 101 38 470 47 Cuts at: 3303 3317 2140 Size: 14 4896 Fragments arranged by size: 4896 470 167 166 162 101 47 38 14 12 EcoRI G'AATT_C Cuts at: 3382 3760 3382 Size: 378 5695 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3253 3253 Size: 6073 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 2116 5220 1678 Size: 438 3104 2531 Fragments arranged by size: 3104 2531 438 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 3053 3085 4714 Size: 32 135 32 566 32 883 32 1629 Cuts at: 4714 4746 4788 4820 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1629 883 566 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2458 3417 3663 669 Size: 988 281 520 959 246 3079 Fragments arranged by size: 3079 988 959 520 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6073 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 3398 3398 Size: 6073 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5187 5371 1305 Size: 3882 184 2007 Fragments arranged by size: 3882 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2129 2878 10 Size: 54 1425 343 297 749 3205 Fragments arranged by size: 3205 1425 749 343 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 4031 4390 4549 518 Size: 648 2865 359 159 2042 Fragments arranged by size: 2865 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 2642 2737 2850 2920 3489 4166 5314 Size: 442 594 95 113 70 569 677 1148 Cuts at: 5314 5559 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 594 569 442 245 113 95 70 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5521 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6073 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NspI r_CATG'y Cuts at: 1132 1229 2423 3431 4976 1132 Size: 97 1194 1008 1545 2229 Fragments arranged by size: 2229 1545 1194 1008 97 PasI CC'CwG_GG Cuts at: 1348 2519 1348 Size: 1171 4902 PciI A'CATG_T Cuts at: 4972 4972 Size: 6073 PflMI CCAn_nnn'nTGG Cuts at: 1108 2125 2446 2478 1108 Size: 1017 321 32 4703 Fragments arranged by size: 4703 1017 321 32 PfoI T'CCnGG_A Cuts at: 2814 2814 Size: 6073 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2468 3256 2468 Size: 788 5285 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6073 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PspOMI G'GGCC_C Cuts at: 2057 2139 2302 2748 2786 3302 3317 2057 Size: 82 163 446 38 516 15 4813 Fragments arranged by size: 4813 516 446 163 82 38 15 PstI C_TGCA'G Cuts at: 830 2886 3345 3425 830 Size: 2056 459 80 3478 Fragments arranged by size: 3478 2056 459 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 PvuII CAG'CTG Cuts at: 2642 2642 Size: 6073 SacII CC_GC'GG Cuts at: 2049 2851 2049 Size: 802 5271 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SapI GCTCTTCn'nnn_ Cuts at: 2007 2007 Size: 6073 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 2491 3147 4321 6033 2491 Size: 656 1174 1712 2531 Fragments arranged by size: 2531 1712 1174 656 SfcI C'TryA_G Cuts at: 826 1045 2882 3341 3421 4556 5237 5428 826 Size: 219 1837 459 80 1135 681 191 1471 Fragments arranged by size: 1837 1471 1135 681 459 219 191 80 SfiI GGCCn_nnn'nGGCC Cuts at: 2033 2033 Size: 6073 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 3400 1872 Size: 1528 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4177 5078 5340 820 Size: 197 926 2234 901 262 1553 Fragments arranged by size: 2234 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3864 493 Size: 3371 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3431 1132 Size: 2299 3774 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 3339 1517 Size: 1822 4251 StyI C'CwwG_G Cuts at: 513 1065 2053 3023 3252 3321 3512 513 Size: 552 988 970 229 69 191 3074 Fragments arranged by size: 3074 988 970 552 229 191 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4100 4117 4270 4455 4900 325 Size: 1255 2520 17 153 185 445 1498 Fragments arranged by size: 2520 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2489 2778 3145 Size: 274 80 33 51 1443 512 289 367 Cuts at: 3145 4319 6031 96 Size: 1174 1712 138 Fragments arranged by size: 1712 1443 1174 512 367 289 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6033 6065 6033 Size: 32 6041 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6073 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6073 XmaI C'CCGG_G Cuts at: 1870 3398 1870 Size: 1528 4545 XmnI GAAnn'nnTTC Cuts at: 4202 4202 Size: 6073 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfoI SrfI SwaI XcmI