Restriction map for
pFN21AA0409
(Linear) (Six-base) MAPSORT of: KIAA0409.seq Check: 1159 from: 1 to: 1368
KIAA0409.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 401 1165 1327 1368
Size: 278 123 764 162 41
Fragments arranged by size:
764 278 162 123 41
AlwNI CAG_nnn'CTG
Cuts at: 0 441 735 1368
Size: 441 294 633
Fragments arranged by size:
633 441 294
ApaI G_GGCC'C
Cuts at: 0 56 138 301 747 785 1301 1316 1368
Size: 56 82 163 446 38 516 15 52
Fragments arranged by size:
516 446 163 82 56 52 38 15
ApoI r'AATT_y
Cuts at: 0 426 1368
Size: 426 942
AvaI C'yCGr_G
Cuts at: 0 698 1368
Size: 698 670
AvrII C'CTAG_G
Cuts at: 0 1018 1368
Size: 1018 350
BanI G'GyrC_C
Cuts at: 0 618 1368
Size: 618 750
BanII G_rGCy'C
Cuts at: 0 56 103 138 301 720 747 785 1301
Size: 56 47 35 163 419 27 38 516
Cuts at: 1301 1316 1368
Size: 15 52
Fragments arranged by size:
516 419 163 56 52 47 38 35
27 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 661 821 1368
Size: 661 160 547
Fragments arranged by size:
661 547 160
BbvCI CC'TCA_GC
Cuts at: 0 638 1172 1368
Size: 638 534 196
Fragments arranged by size:
638 534 196
BciVI GTATCCnnnnn_n'
Cuts at: 0 985 1368
Size: 985 383
BglI GCCn_nnn'nGGC
Cuts at: 0 28 142 724 1368
Size: 28 114 582 644
Fragments arranged by size:
644 582 114 28
BlpI GC'TnA_GC
Cuts at: 0 1322 1368
Size: 1322 46
Bme1580I G_kGCm'C
Cuts at: 0 56 138 301 747 785 1301 1316 1368
Size: 56 82 163 446 38 516 15 52
Fragments arranged by size:
516 446 163 82 56 52 38 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 28 1304 1368
Size: 28 1276 64
Fragments arranged by size:
1276 64 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 1368
Size: 1075 293
Bpu10I CC'TnA_GC
Cuts at: 0 638 1172 1368
Size: 638 534 196
Fragments arranged by size:
638 534 196
BsaI GGTCTCn'nnnn_
Cuts at: 0 1162 1368
Size: 1162 206
BsaWI w'CCGG_w
Cuts at: 0 977 1368
Size: 977 391
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 1048 1078 1368
Size: 85 30 933 30 290
Fragments arranged by size:
933 290 85 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 208 211 214 217 1141 1368
Size: 75 133 3 3 3 924 227
Fragments arranged by size:
924 227 133 75 3 3 3
BseYI C'CCAG_C
Cuts at: 0 158 169 434 633 784 1368
Size: 158 11 265 199 151 584
Fragments arranged by size:
584 265 199 158 151 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1368
Size: 779 589
BsmBI CGTCTCn'nnnn_
Cuts at: 0 808 1368
Size: 808 560
Bsp1286I G_dGCh'C
Cuts at: 0 56 103 138 301 720 747 785 1301
Size: 56 47 35 163 419 27 38 516
Cuts at: 1301 1316 1368
Size: 15 52
Fragments arranged by size:
516 419 163 56 52 47 38 35
27 15
BspEI T'CCGG_A
Cuts at: 0 977 1368
Size: 977 391
BsrDI GCAATG_nn'
Cuts at: 0 990 1368
Size: 990 378
BsrFI r'CCGG_y
Cuts at: 0 569 1368
Size: 569 799
BstAPI GCAn_nnn'nTGC
Cuts at: 0 241 1368
Size: 241 1127
BstBI TT'CG_AA
Cuts at: 0 5 1200 1368
Size: 5 1195 168
Fragments arranged by size:
1195 168 5
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1294 1368
Size: 1294 74
BstYI r'GATC_y
Cuts at: 0 903 1368
Size: 903 465
Bsu36I CC'TnA_GG
Cuts at: 0 614 821 1368
Size: 614 207 547
Fragments arranged by size:
614 547 207
BtgI C'CryG_G
Cuts at: 0 41 843 1368
Size: 41 802 525
Fragments arranged by size:
802 525 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 879 1368
Size: 879 489
BtsI GCAGTG_nn'
Cuts at: 0 315 375 565 795 874 1368
Size: 315 60 190 230 79 494
Fragments arranged by size:
494 315 230 190 79 60
DraIII CAC_nnn'GTG
Cuts at: 0 1031 1368
Size: 1031 337
DrdI GACnn_nn'nnGTC
Cuts at: 0 663 1021 1368
Size: 663 358 347
Fragments arranged by size:
663 358 347
EaeI y'GGCC_r
Cuts at: 0 122 871 1368
Size: 122 749 497
Fragments arranged by size:
749 497 122
EarI CTCTTCn'nnn_
Cuts at: 0 2 811 1368
Size: 2 809 557
Fragments arranged by size:
809 557 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 62 579 1368
Size: 62 517 789
Fragments arranged by size:
789 517 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 401 1075 1165 1327 1368
Size: 278 123 674 90 162 41
Fragments arranged by size:
674 278 162 123 90 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1126 1307 1368
Size: 1126 181 61
Fragments arranged by size:
1126 181 61
EcoO109I rG'GnC_Cy
Cuts at: 0 135 297 463 630 642 743 781 1251
Size: 135 162 166 167 12 101 38 470
Cuts at: 1251 1298 1312 1368
Size: 47 14 56
Fragments arranged by size:
470 167 166 162 135 101 56 47
38 14 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1248 1368
Size: 1248 120
HaeII r_GCGC'y
Cuts at: 0 111 1368
Size: 111 1257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 1048 1080 1368
Size: 133 32 883 32 288
Fragments arranged by size:
883 288 133 32 32
HincII GTy'rAC
Cuts at: 0 453 1368
Size: 453 915
MscI TGG'CCA
Cuts at: 0 124 873 1368
Size: 124 749 495
Fragments arranged by size:
749 495 124
MspA1I CmG'CkG
Cuts at: 0 43 637 732 845 915 1368
Size: 43 594 95 113 70 453
Fragments arranged by size:
594 453 113 95 70 43
NspI r_CATG'y
Cuts at: 0 418 1368
Size: 418 950
PasI CC'CwG_GG
Cuts at: 0 514 1368
Size: 514 854
PflMI CCAn_nnn'nTGG
Cuts at: 0 120 441 473 1368
Size: 120 321 32 895
Fragments arranged by size:
895 321 120 32
PfoI T'CCnGG_A
Cuts at: 0 809 1368
Size: 809 559
PpuMI rG'GwC_Cy
Cuts at: 0 463 1251 1368
Size: 463 788 117
Fragments arranged by size:
788 463 117
PspOMI G'GGCC_C
Cuts at: 0 52 134 297 743 781 1297 1312 1368
Size: 52 82 163 446 38 516 15 56
Fragments arranged by size:
516 446 163 82 56 52 38 15
PstI C_TGCA'G
Cuts at: 0 881 1340 1368
Size: 881 459 28
Fragments arranged by size:
881 459 28
PvuII CAG'CTG
Cuts at: 0 637 1368
Size: 637 731
SacII CC_GC'GG
Cuts at: 0 44 846 1368
Size: 44 802 522
Fragments arranged by size:
802 522 44
SapI GCTCTTCn'nnn_
Cuts at: 0 2 1368
Size: 2 1366
ScaI AGT'ACT
Cuts at: 0 486 1142 1368
Size: 486 656 226
Fragments arranged by size:
656 486 226
SfcI C'TryA_G
Cuts at: 0 877 1336 1368
Size: 877 459 32
Fragments arranged by size:
877 459 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 28 1368
Size: 28 1340
StuI AGG'CCT
Cuts at: 0 1334 1368
Size: 1334 34
StyI C'CwwG_G
Cuts at: 0 48 1018 1247 1316 1368
Size: 48 970 229 69 52
Fragments arranged by size:
970 229 69 52 48
TatI w'GTAC_w
Cuts at: 0 484 773 1140 1368
Size: 484 289 367 228
Fragments arranged by size:
484 367 289 228
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI AvrII BanI BanII
BbsI BbvCI BciVI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsmBI
Bsp1286I BspEI BsrDI BsrFI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
MscI MspA1I NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacII SapI ScaI SfcI SfiI StuI
StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI BaeI BamHI BbeI BcgI BclI BfrBI BglII
BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsiEI
BsiHKAI BsiWI BsmI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstEII BstZ17I ClaI CspCI DraI EagI EcoICRI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SbfI SexAI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI