(Circular) (Six-base) MAPSORT of: pFN21AA0405.seq Check: 4982 from: 1 to: 6685 pFN21AA0405 6685 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3605 4495 278 Size: 53 83 186 936 2069 890 2468 Fragments arranged by size: 2468 2069 936 890 186 83 53 AccI GT'mk_AC Cuts at: 1937 2145 2283 4028 1937 Size: 208 138 1745 4594 Fragments arranged by size: 4594 1745 208 138 Acc65I G'GTAC_C Cuts at: 4006 4006 Size: 6685 AclI AA'CG_TT Cuts at: 1563 4812 5185 1563 Size: 3249 373 3063 Fragments arranged by size: 3249 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2665 2930 4748 6132 6400 1632 Size: 440 593 265 1818 1384 268 1917 Fragments arranged by size: 1917 1818 1384 593 440 268 265 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6488 AflIII A'CryG_T Cuts at: 1203 5584 6561 1203 Size: 4381 977 1327 Fragments arranged by size: 4381 1327 977 AgeI A'CCGG_T Cuts at: 5516 5516 Size: 6685 AhdI GACnn_n'nnGTC Cuts at: 5414 5414 Size: 6685 AleI CACnn'nnGTG Cuts at: 1166 3365 1166 Size: 2199 4486 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2937 1184 Size: 1753 4932 AlwNI CAG_nnn'CTG Cuts at: 4428 6000 4428 Size: 1572 5113 ApaLI G'TGCA_C Cuts at: 2273 4742 5898 2273 Size: 2469 1156 3060 Fragments arranged by size: 3060 2469 1156 ApoI r'AATT_y Cuts at: 1493 2309 2705 3994 4220 4372 6481 1493 Size: 816 396 1289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1289 816 396 226 152 AseI AT'TA_AT Cuts at: 160 1032 2229 3906 5239 160 Size: 872 1197 1677 1333 1606 Fragments arranged by size: 1677 1606 1333 1197 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 AvaI C'yCGr_G Cuts at: 1870 1943 4010 1870 Size: 73 2067 4545 Fragments arranged by size: 4545 2067 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2647 2680 2647 Size: 33 6652 BamHI G'GATC_C Cuts at: 2184 3276 4015 4382 2184 Size: 1092 739 367 4487 Fragments arranged by size: 4487 1092 739 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2675 3779 4006 5461 618 Size: 325 217 627 888 1104 227 1455 1842 Fragments arranged by size: 1842 1455 1104 888 627 325 227 217 BanII G_rGCy'C Cuts at: 1391 2082 3459 3620 4004 1391 Size: 691 1377 161 384 4072 Fragments arranged by size: 4072 1377 691 384 161 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2463 3525 928 Size: 971 564 1062 4088 Fragments arranged by size: 4088 1062 971 564 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4876 4910 1903 Size: 34 2939 34 3678 Fragments arranged by size: 3678 2939 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4577 5787 4577 Size: 1210 5475 BclI T'GATC_A Cuts at: 1548 2766 1548 Size: 1218 5467 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2533 5296 136 Size: 107 122 71 631 605 861 2763 1525 Fragments arranged by size: 2763 1525 861 631 605 122 107 71 BglII A'GATC_T Cuts at: 6680 6680 Size: 6685 BlpI GC'TnA_GC Cuts at: 3680 4102 3680 Size: 422 6263 Bme1580I G_kGCm'C Cuts at: 1790 2277 2678 4746 5902 1790 Size: 487 401 2068 1156 2573 Fragments arranged by size: 2573 2068 1156 487 401 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6685 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3244 4416 5374 Size: 416 53 83 399 24 1827 1172 958 Cuts at: 5374 442 Size: 1753 Fragments arranged by size: 1827 1753 1172 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6685 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3824 3989 5345 1239 Size: 2585 165 1356 2579 Fragments arranged by size: 2585 2579 1356 165 Bpu10I CC'TnA_GC Cuts at: 2516 3437 2516 Size: 921 5764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2684 2865 4810 5675 5973 2684 Size: 181 1945 865 298 3396 Fragments arranged by size: 3396 1945 865 298 181 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5348 882 Size: 617 3849 2219 Fragments arranged by size: 3849 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2720 4476 493 Size: 711 1516 1756 2702 Fragments arranged by size: 2702 1756 1516 711 BsaBI GATnn'nnATC Cuts at: 2571 6679 2571 Size: 4108 2577 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3602 4492 4874 5509 Size: 53 83 186 936 2069 890 382 635 Cuts at: 5509 275 Size: 1451 Fragments arranged by size: 2069 1451 936 890 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5118 5516 5790 5937 6348 5118 Size: 398 274 147 411 5455 Fragments arranged by size: 5455 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2196 2226 3121 3136 3151 Size: 30 165 30 793 30 895 15 15 Cuts at: 3151 3166 1178 Size: 15 4697 Fragments arranged by size: 4697 895 793 165 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2730 1186 Size: 1544 5141 BseYI C'CCAG_C Cuts at: 2030 2394 3118 5888 6384 2030 Size: 364 724 2770 496 2331 Fragments arranged by size: 2770 2331 724 496 364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2240 2402 1163 Size: 573 504 162 5446 Fragments arranged by size: 5446 573 504 162 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4135 4896 5924 1540 Size: 275 186 2134 761 1028 2301 Fragments arranged by size: 2301 2134 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 3975 4746 4831 5902 2277 Size: 1698 771 85 1071 3060 Fragments arranged by size: 3060 1698 1071 771 85 BsmI GAATG_Cn' Cuts at: 4196 4289 4196 Size: 93 6592 BsmBI CGTCTCn'nnnn_ Cuts at: 3838 3838 Size: 6685 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2277 2678 3459 3620 3975 4004 Size: 399 292 195 401 781 161 355 29 Cuts at: 4004 4746 4831 5902 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 781 742 401 399 355 292 195 161 85 29 BspEI T'CCGG_A Cuts at: 6348 6348 Size: 6685 BspHI T'CATG_A Cuts at: 4574 6304 4574 Size: 1730 4955 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4040 844 Size: 3196 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4572 1394 Size: 429 2749 3507 Fragments arranged by size: 3507 2749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5180 5354 65 Size: 1183 3932 174 1396 Fragments arranged by size: 3932 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3750 5329 5516 1700 Size: 110 1940 1579 187 2869 Fragments arranged by size: 2869 1940 1579 187 110 BsrGI T'GTAC_A Cuts at: 96 2263 96 Size: 2167 4518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6685 BssSI C'ACGA_G Cuts at: 2614 4745 5757 2614 Size: 2131 1012 3542 Fragments arranged by size: 3542 2131 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6685 BstBI TT'CG_AA Cuts at: 5489 5489 Size: 6685 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6685 BstXI CCAn_nnnn'nTGG Cuts at: 2104 2104 Size: 6685 BstYI r'GATC_y Cuts at: 1972 2184 3276 4015 4382 4767 4784 6236 6322 Size: 212 1092 739 367 385 17 1452 86 Cuts at: 6322 6334 6680 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1092 739 385 367 346 212 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2035 513 Size: 552 939 31 5163 Fragments arranged by size: 5163 939 552 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5946 Fragments arranged by size: 5946 683 56 BtsI GCAGTG_nn' Cuts at: 2527 2919 3973 4201 4995 5015 2527 Size: 392 1054 228 794 20 4197 Fragments arranged by size: 4197 1054 794 392 228 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6685 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6650 DraI TTT'AAA Cuts at: 3989 3989 Size: 6685 DraIII CAC_nnn'GTG Cuts at: 2025 2025 Size: 6685 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5692 809 Size: 507 4376 1802 Fragments arranged by size: 4376 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3048 4132 5021 Size: 54 1425 50 275 18 1218 1084 889 Cuts at: 5021 6650 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1218 1084 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4132 1537 Size: 275 2320 4090 Fragments arranged by size: 4090 2320 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 3116 3341 4615 1353 Size: 1053 710 225 1274 3423 Fragments arranged by size: 3423 1274 1053 710 225 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3769 5272 5646 5792 387 Size: 1064 138 2180 1503 374 146 1280 Fragments arranged by size: 2180 1503 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2665 2930 3824 3989 4748 5345 Size: 393 440 593 265 894 165 759 597 Cuts at: 5345 6132 6400 1239 Size: 787 268 1524 Fragments arranged by size: 1524 894 787 759 597 593 440 393 268 265 165 EcoO109I rG'GnC_Cy Cuts at: 2463 3026 2463 Size: 563 6122 EcoRI G'AATT_C Cuts at: 2309 3994 4372 2309 Size: 1685 378 4622 Fragments arranged by size: 4622 1685 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3150 3150 Size: 6685 FspI TGC'GCA Cuts at: 5191 5191 Size: 6685 HaeII r_GCGC'y Cuts at: 1678 3718 5832 1678 Size: 2040 2114 2531 Fragments arranged by size: 2531 2114 2040 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2417 2449 2863 2885 2895 Size: 32 135 32 845 32 414 22 10 Cuts at: 2895 2917 3446 3478 5326 5358 5400 5432 1373 Size: 22 529 32 1848 32 42 32 2626 Fragments arranged by size: 2626 1848 845 529 414 135 42 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 3448 4029 4275 669 Size: 988 281 1510 581 246 3079 Fragments arranged by size: 3079 1510 988 581 281 246 HindIII A'AGCT_T Cuts at: 748 2161 2405 748 Size: 1413 244 5028 Fragments arranged by size: 5028 1413 244 HpaI GTT'AAC Cuts at: 3448 4275 3448 Size: 827 5858 KpnI G_GTAC'C Cuts at: 4010 4010 Size: 6685 MfeI C'AATT_G Cuts at: 2712 4284 2712 Size: 1572 5113 MluI A'CGCG_T Cuts at: 6561 6561 Size: 6685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2616 2880 2928 3164 3221 5799 5983 1305 Size: 1311 264 48 236 57 2578 184 2007 Fragments arranged by size: 2578 2007 1311 264 236 184 57 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4863 Fragments arranged by size: 4863 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2424 3344 3365 4643 5002 5161 518 Size: 648 1258 920 21 1278 359 159 2042 Fragments arranged by size: 2042 1278 1258 920 648 359 159 21 MspA1I CmG'CkG Cuts at: 1606 2127 3324 3420 4101 4778 5926 6171 1606 Size: 521 1197 96 681 677 1148 245 2120 Fragments arranged by size: 2120 1197 1148 681 677 521 245 96 NaeI GCC'GGC Cuts at: 3752 3752 Size: 6685 NcoI C'CATG_G Cuts at: 513 1065 2004 2035 513 Size: 552 939 31 5163 Fragments arranged by size: 5163 939 552 31 NdeI CA'TA_TG Cuts at: 387 3699 387 Size: 3312 3373 NgoMIV G'CCGG_C Cuts at: 3750 3750 Size: 6685 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6685 NotI GC'GGCC_GC Cuts at: 4132 4132 Size: 6685 NspI r_CATG'y Cuts at: 1132 1229 3940 4043 5588 1132 Size: 97 2711 103 1545 2229 Fragments arranged by size: 2711 2229 1545 103 97 PasI CC'CwG_GG Cuts at: 1348 2074 1348 Size: 726 5959 PciI A'CATG_T Cuts at: 5584 5584 Size: 6685 PflMI CCAn_nnn'nTGG Cuts at: 1108 2025 3613 1108 Size: 917 1588 4180 Fragments arranged by size: 4180 1588 917 PfoI T'CCnGG_A Cuts at: 2187 2469 3801 2187 Size: 282 1332 5071 Fragments arranged by size: 5071 1332 282 PmeI GTTT'AAAC Cuts at: 3989 3989 Size: 6685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4754 4786 6267 6299 4754 Size: 32 1481 32 5140 Fragments arranged by size: 5140 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3026 3026 Size: 6685 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6685 PsiI TTA'TAA Cuts at: 4255 4255 Size: 6685 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2129 2161 2129 Size: 32 6653 PstI C_TGCA'G Cuts at: 830 2251 2954 3879 4037 830 Size: 1421 703 925 158 3478 Fragments arranged by size: 3478 1421 925 703 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6685 PvuII CAG'CTG Cuts at: 3324 3324 Size: 6685 SalI G'TCGA_C Cuts at: 1936 4027 1936 Size: 2091 4594 SapI GCTCTTCn'nnn_ Cuts at: 2406 2406 Size: 6685 SbfI CC_TGCA'GG Cuts at: 4037 4037 Size: 6685 ScaI AGT'ACT Cuts at: 4933 6645 4933 Size: 1712 4973 SfcI C'TryA_G Cuts at: 826 1045 2012 2247 2950 3875 4033 5168 5849 Size: 219 967 235 703 925 158 1135 681 Cuts at: 5849 6040 826 Size: 191 1471 Fragments arranged by size: 1471 1135 967 925 703 681 235 219 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6685 SmaI CCC'GGG Cuts at: 1872 4012 1872 Size: 2140 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2699 2880 4789 5690 5952 820 Size: 197 926 756 181 1909 901 262 1553 Fragments arranged by size: 1909 1553 926 901 756 262 197 181 SnaBI TAC'GTA Cuts at: 493 2720 4476 493 Size: 2227 1756 2702 Fragments arranged by size: 2702 2227 1756 SpeI A'CTAG_T Cuts at: 152 152 Size: 6685 SphI G_CATG'C Cuts at: 1132 4043 1132 Size: 2911 3774 SspI AAT'ATT Cuts at: 5 52 2365 4609 5 Size: 47 2313 2244 2081 Fragments arranged by size: 2313 2244 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6685 StyI C'CwwG_G Cuts at: 513 1065 2004 2035 2936 3022 4124 513 Size: 552 939 31 901 86 1102 3074 Fragments arranged by size: 3074 1102 939 901 552 86 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3355 3560 4712 4729 4882 5067 5512 Size: 1255 1775 205 1152 17 153 185 445 Cuts at: 5512 325 Size: 1498 Fragments arranged by size: 1775 1498 1255 1152 445 205 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2263 3128 4931 Size: 274 80 33 51 1443 286 865 1803 Cuts at: 4931 6643 96 Size: 1712 138 Fragments arranged by size: 1803 1712 1443 865 286 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3136 3168 6645 6677 3136 Size: 32 3477 32 3144 Fragments arranged by size: 3477 3144 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3601 1316 Size: 2285 4400 XbaI T'CTAG_A Cuts at: 4021 4021 Size: 6685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2443 2449 2443 Size: 6 6679 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6685 XmaI C'CCGG_G Cuts at: 1870 4010 1870 Size: 2140 4545 XmnI GAAnn'nnTTC Cuts at: 4814 4814 Size: 6685 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3603 4493 276 Size: 53 83 186 936 2069 890 2468 Fragments arranged by size: 2468 2069 936 890 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI