(Circular) (Six-base) MAPSORT of: pFN21AA0404.seq Check: 1710 from: 1 to: 10519 pFN21AA0404 10519 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2871 4103 6238 2871 Size: 1232 2135 7152 Fragments arranged by size: 7152 2135 1232 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3329 8329 278 Size: 53 83 186 936 1793 5000 2468 Fragments arranged by size: 5000 2468 1793 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3165 4491 5943 7862 1937 Size: 1228 1326 1452 1919 4594 Fragments arranged by size: 4594 1919 1452 1326 1228 Acc65I G'GTAC_C Cuts at: 4898 7840 4898 Size: 2942 7577 AclI AA'CG_TT Cuts at: 1563 8646 9019 1563 Size: 7083 373 3063 Fragments arranged by size: 7083 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2879 3305 4823 4891 5889 6979 7145 8582 Size: 1247 426 1518 68 998 1090 166 1437 Cuts at: 8582 9966 10234 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1518 1437 1384 1247 1090 998 426 268 166 68 AfeI AGC'GCT Cuts at: 6690 7502 6690 Size: 812 9707 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10322 AflIII A'CryG_T Cuts at: 1203 5136 5659 9418 10395 1203 Size: 3933 523 3759 977 1327 Fragments arranged by size: 3933 3759 1327 977 523 AgeI A'CCGG_T Cuts at: 7410 9350 7410 Size: 1940 8579 AhdI GACnn_n'nnGTC Cuts at: 3516 7459 9248 3516 Size: 3943 1789 4787 Fragments arranged by size: 4787 3943 1789 AleI CACnn'nnGTG Cuts at: 1166 2528 3485 5150 5670 6257 6639 1166 Size: 1362 957 1665 520 587 382 5046 Fragments arranged by size: 5046 1665 1362 957 587 520 382 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3503 3563 5688 6233 1184 Size: 2319 60 2125 545 5470 Fragments arranged by size: 5470 2319 2125 545 60 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4217 4249 4217 Size: 32 10487 AlwNI CAG_nnn'CTG Cuts at: 2348 2452 2596 2902 3004 3016 3035 3694 6515 Size: 104 144 306 102 12 19 659 2821 Cuts at: 6515 6535 6580 7388 8262 9834 2348 Size: 20 45 808 874 1572 3033 Fragments arranged by size: 3033 2821 1572 874 808 659 306 144 104 102 45 20 19 12 ApaI G_GGCC'C Cuts at: 3402 3802 5974 6002 7253 3402 Size: 400 2172 28 1251 6668 Fragments arranged by size: 6668 2172 1251 400 28 ApaLI G'TGCA_C Cuts at: 3548 4865 5663 8576 9732 3548 Size: 1317 798 2913 1156 4335 Fragments arranged by size: 4335 2913 1317 1156 798 ApoI r'AATT_y Cuts at: 1493 7828 8054 8206 10315 1493 Size: 6335 226 152 2109 1697 Fragments arranged by size: 6335 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 9073 160 Size: 872 8041 1606 Fragments arranged by size: 8041 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10519 AvaI C'yCGr_G Cuts at: 1870 1943 2875 3411 3588 3921 4582 5104 5246 Size: 73 932 536 177 333 661 522 142 Cuts at: 5246 6126 6255 6869 6876 7739 7844 1870 Size: 880 129 614 7 863 105 4545 Fragments arranged by size: 4545 932 880 863 661 614 536 522 333 177 142 129 105 73 7 BamHI G'GATC_C Cuts at: 7849 8216 7849 Size: 367 10152 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2635 2972 3593 4898 6226 Size: 325 217 627 848 337 621 1305 1328 Cuts at: 6226 6288 7655 7840 9295 618 Size: 62 1367 185 1455 1842 Fragments arranged by size: 1842 1455 1367 1328 1305 848 627 621 337 325 217 185 62 BanII G_rGCy'C Cuts at: 1391 2240 2433 2721 2748 2855 3042 3150 3402 Size: 849 193 288 27 107 187 108 252 Cuts at: 3402 3647 3802 3876 4032 4326 5974 6002 6365 Size: 245 155 74 156 294 1648 28 363 Cuts at: 6365 6883 7158 7253 7838 1391 Size: 518 275 95 585 4072 Fragments arranged by size: 4072 1648 849 585 518 363 294 288 275 252 245 193 187 156 155 108 107 95 74 28 27 BbeI G_GCGC'C Cuts at: 2639 7659 2639 Size: 5020 5499 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2985 4754 928 Size: 971 1086 1769 6693 Fragments arranged by size: 6693 1769 1086 971 BbvCI CC'TCA_GC Cuts at: 2102 3044 3133 3655 6858 2102 Size: 942 89 522 3203 5763 Fragments arranged by size: 5763 3203 942 522 89 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3365 3399 5666 5700 8710 8744 1903 Size: 34 1428 34 2267 34 3010 34 3678 Fragments arranged by size: 3678 3010 2267 1428 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7588 8411 9621 7588 Size: 823 1210 8486 Fragments arranged by size: 8486 1210 823 BclI T'GATC_A Cuts at: 1548 1548 Size: 10519 BfrBI ATG'CAT Cuts at: 2376 2376 Size: 10519 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3998 4602 4751 Size: 107 122 71 631 605 2326 604 149 Cuts at: 4751 5115 6006 6760 7721 9130 136 Size: 364 891 754 961 1409 1525 Fragments arranged by size: 2326 1525 1409 961 891 754 631 605 604 364 149 122 107 71 BglII A'GATC_T Cuts at: 4546 10514 4546 Size: 5968 4551 BlpI GC'TnA_GC Cuts at: 2117 2906 3036 3846 4002 4049 5049 6461 7936 Size: 789 130 810 156 47 1000 1412 1475 Cuts at: 7936 2117 Size: 4700 Fragments arranged by size: 4700 1475 1412 1000 810 789 156 130 47 Bme1580I G_kGCm'C Cuts at: 1790 2259 3402 3482 3552 3596 3802 4869 5667 Size: 469 1143 80 70 44 206 1067 798 Cuts at: 5667 5689 5816 5974 6002 6229 6249 6664 7076 Size: 22 127 158 28 227 20 415 412 Cuts at: 7076 7253 7813 8580 9736 1790 Size: 177 560 767 1156 2573 Fragments arranged by size: 2573 1156 1143 1067 798 767 560 469 415 412 227 206 177 158 127 80 70 44 28 22 20 BmgBI CAC'GTC Cuts at: 1318 3787 5287 6659 7014 7054 7618 7728 1318 Size: 2469 1500 1372 355 40 564 110 4109 Fragments arranged by size: 4109 2469 1500 1372 564 355 110 40 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4027 4064 4194 Size: 416 53 83 399 24 2610 37 130 Cuts at: 4194 4225 4452 4912 5007 5025 5260 5384 5813 Size: 31 227 460 95 18 235 124 429 Cuts at: 5813 6236 8250 9208 442 Size: 423 2014 958 1753 Fragments arranged by size: 2610 2014 1753 958 460 429 423 416 399 235 227 130 124 95 83 53 37 31 24 18 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10519 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6614 6614 Size: 10519 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2234 2291 2474 2483 2618 2724 2870 Size: 974 21 57 183 9 135 106 146 Cuts at: 2870 3584 3593 3837 4037 4301 4401 4877 5413 Size: 714 9 244 200 264 100 476 536 Cuts at: 5413 5690 6557 6602 9179 1239 Size: 277 867 45 2577 2579 Fragments arranged by size: 2579 2577 974 867 714 536 476 277 264 244 200 183 146 135 106 100 57 45 21 9 9 Bpu10I CC'TnA_GC Cuts at: 2102 2770 3044 3133 3655 3966 4320 5465 6013 Size: 668 274 89 522 311 354 1145 548 Cuts at: 6013 6858 2102 Size: 845 5763 Fragments arranged by size: 5763 1145 845 668 548 522 354 311 274 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6779 7775 8644 9509 9807 6779 Size: 996 869 865 298 7491 Fragments arranged by size: 7491 996 869 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4377 4823 5408 5513 6280 6585 6842 Size: 617 2878 446 585 105 767 305 257 Cuts at: 6842 6917 7436 9182 882 Size: 75 519 1746 2219 Fragments arranged by size: 2878 2219 1746 767 617 585 519 446 305 257 105 75 BsaAI yAC'GTr Cuts at: 493 1204 5662 5713 6269 8310 493 Size: 711 4458 51 556 2041 2702 Fragments arranged by size: 4458 2702 2041 711 556 51 BsaBI GATnn'nnATC Cuts at: 2652 4373 10513 2652 Size: 1721 6140 2658 Fragments arranged by size: 6140 2658 1721 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2636 3326 5859 5927 Size: 53 83 186 936 1103 690 2533 68 Cuts at: 5927 7656 7770 8326 8708 9343 275 Size: 1729 114 556 382 635 1451 Fragments arranged by size: 2533 1729 1451 1103 936 690 635 556 382 186 114 83 68 53 BsaWI w'CCGG_w Cuts at: 2425 3341 4038 7410 8952 9350 9624 9771 10182 Size: 916 697 3372 1542 398 274 147 411 Cuts at: 10182 2425 Size: 2762 Fragments arranged by size: 3372 2762 1542 916 697 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3158 3182 3188 3212 3566 Size: 30 165 30 1755 24 6 24 354 Cuts at: 3566 3596 5339 5369 5370 5400 6950 6980 7712 Size: 30 1743 30 1 30 1550 30 732 Cuts at: 7712 7742 1178 Size: 30 3955 Fragments arranged by size: 3955 1755 1743 1550 732 354 165 30 30 30 30 30 30 30 24 24 6 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2729 3166 4327 4379 4406 6044 6068 6554 Size: 1543 437 1161 52 27 1638 24 486 Cuts at: 6554 6595 7079 1186 Size: 41 484 4626 Fragments arranged by size: 4626 1638 1543 1161 486 484 437 52 41 27 24 BseYI C'CCAG_C Cuts at: 2364 2848 3226 3640 3793 4478 4575 5305 5753 Size: 484 378 414 153 685 97 730 448 Cuts at: 5753 5898 6248 6493 6524 6882 6887 7177 7237 Size: 145 350 245 31 358 5 290 60 Cuts at: 7237 9722 10218 2364 Size: 2485 496 2665 Fragments arranged by size: 2665 2485 730 685 496 484 448 414 378 358 350 290 245 153 145 97 60 31 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 2269 2609 2657 2690 2843 3509 Size: 573 315 218 340 48 33 153 666 Cuts at: 3509 3900 5324 5717 6593 1163 Size: 391 1424 393 876 5089 Fragments arranged by size: 5089 1424 876 666 573 393 391 340 315 218 153 48 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4992 6200 6204 6548 7969 8730 Size: 275 186 2991 1208 4 344 1421 761 Cuts at: 8730 9758 1540 Size: 1028 2301 Fragments arranged by size: 2991 2301 1421 1208 1028 761 344 275 186 4 BsiHKAI G_wGCw'C Cuts at: 2100 2544 2721 3150 3552 4184 4778 4869 4896 Size: 444 177 429 402 632 594 91 27 Cuts at: 4896 5603 5667 5723 6365 8580 8665 9736 2100 Size: 707 64 56 642 2215 85 1071 2883 Fragments arranged by size: 2883 2215 1071 707 642 632 594 444 429 402 177 91 85 64 56 27 BsmI GAATG_Cn' Cuts at: 5260 8030 8123 5260 Size: 2770 93 7656 Fragments arranged by size: 7656 2770 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4918 5819 6748 7483 4918 Size: 901 929 735 7954 Fragments arranged by size: 7954 929 901 735 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2240 2259 2433 2544 2721 2748 Size: 399 310 140 19 174 111 177 27 Cuts at: 2748 2855 3042 3150 3402 3482 3552 3596 3647 Size: 107 187 108 252 80 70 44 51 Cuts at: 3647 3802 3876 4032 4184 4326 4778 4869 4896 Size: 155 74 156 152 142 452 91 27 Cuts at: 4896 5603 5667 5689 5723 5816 5974 6002 6229 Size: 707 64 22 34 93 158 28 227 Cuts at: 6229 6249 6365 6664 6883 7076 7158 7253 7813 Size: 20 116 299 219 193 82 95 560 Cuts at: 7813 7838 8580 8665 9736 1391 Size: 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 707 560 452 399 310 299 252 227 219 193 187 177 174 158 156 155 152 142 140 116 111 108 107 95 93 91 85 82 80 74 70 64 51 44 34 28 27 27 25 22 20 19 BspEI T'CCGG_A Cuts at: 3341 10182 3341 Size: 6841 3678 BspHI T'CATG_A Cuts at: 8408 10138 8408 Size: 1730 8789 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2871 3850 3966 4103 4533 5322 5538 5709 Size: 2027 979 116 137 430 789 216 171 Cuts at: 5709 6238 7302 7551 7874 844 Size: 529 1064 249 323 3489 Fragments arranged by size: 3489 2027 1064 979 789 529 430 323 249 216 171 137 116 BsrBI CCG'CTC Cuts at: 1394 1823 2047 3970 4988 5095 6646 7485 7705 Size: 429 224 1923 1018 107 1551 839 220 Cuts at: 7705 8406 1394 Size: 701 3507 Fragments arranged by size: 3507 1923 1551 1018 839 701 429 224 220 107 BsrDI GCAATG_nn' Cuts at: 65 1248 3739 3948 9014 9188 65 Size: 1183 2491 209 5066 174 1396 Fragments arranged by size: 5066 2491 1396 1183 209 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2641 3204 3525 3807 3857 3880 5771 Size: 110 831 563 321 282 50 23 1891 Cuts at: 5771 6390 6594 6821 7410 9163 9350 1700 Size: 619 204 227 589 1753 187 2869 Fragments arranged by size: 2869 1891 1753 831 619 589 563 321 282 227 204 187 110 50 23 BsrGI T'GTAC_A Cuts at: 96 2130 96 Size: 2034 8485 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10519 BssSI C'ACGA_G Cuts at: 2930 5809 6482 6637 8579 9591 2930 Size: 2879 673 155 1942 1012 3858 Fragments arranged by size: 3858 2879 1942 1012 673 155 BstAPI GCAn_nnn'nTGC Cuts at: 794 2680 794 Size: 1886 8633 BstBI TT'CG_AA Cuts at: 9323 9323 Size: 10519 BstEII G'GTnAC_C Cuts at: 1184 5086 1184 Size: 3902 6617 BstXI CCAn_nnnn'nTGG Cuts at: 2248 3230 4084 4168 4925 5020 5653 7033 2248 Size: 982 854 84 757 95 633 1380 5734 Fragments arranged by size: 5734 1380 982 854 757 633 95 84 BstYI r'GATC_y Cuts at: 1972 3618 3690 4546 6453 7849 8216 8601 8618 Size: 1646 72 856 1907 1396 367 385 17 Cuts at: 8618 10070 10156 10168 10514 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1907 1646 1452 1396 856 385 367 346 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 4389 4600 4749 5922 4389 Size: 211 149 1173 8986 Fragments arranged by size: 8986 1173 211 149 BtgI C'CryG_G Cuts at: 513 1065 2024 3105 4992 5409 5760 6834 7042 Size: 552 959 1081 1887 417 351 1074 208 Cuts at: 7042 7659 7716 513 Size: 617 57 3316 Fragments arranged by size: 3316 1887 1081 1074 959 617 552 417 351 208 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3899 6075 7608 487 Size: 683 56 2673 2176 1533 3398 Fragments arranged by size: 3398 2673 2176 1533 683 56 BtsI GCAGTG_nn' Cuts at: 2281 2622 3036 3130 4060 5145 5201 8035 8829 Size: 341 414 94 930 1085 56 2834 794 Cuts at: 8829 8849 2281 Size: 20 3951 Fragments arranged by size: 3951 2834 1085 930 794 414 341 94 56 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10519 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3108 3143 6700 6735 529 Size: 35 2544 35 3557 35 4313 Fragments arranged by size: 4313 3557 2544 35 35 35 DraI TTT'AAA Cuts at: 7823 7823 Size: 10519 DraIII CAC_nnn'GTG Cuts at: 4248 6487 4248 Size: 2239 8280 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9526 809 Size: 507 8210 1802 Fragments arranged by size: 8210 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2021 2250 2571 Size: 54 1425 50 275 18 191 229 321 Cuts at: 2571 3768 3813 3831 4650 4927 4989 5773 5853 Size: 1197 45 18 819 277 62 784 80 Cuts at: 5853 5911 6197 6325 6819 7330 7557 7713 7966 Size: 58 286 128 494 511 227 156 253 Cuts at: 7966 8855 10484 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1197 889 819 784 511 494 321 286 277 275 253 229 227 191 156 128 80 62 58 54 50 45 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4989 6197 7966 1537 Size: 275 3177 1208 1769 4090 Fragments arranged by size: 4090 3177 1769 1208 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2727 3101 4910 6045 6791 7280 8449 1353 Size: 1374 374 1809 1135 746 489 1169 3423 Fragments arranged by size: 3423 1809 1374 1169 1135 746 489 374 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2690 3544 3797 4825 5476 7664 Size: 1064 138 1101 854 253 1028 651 2188 Cuts at: 7664 9106 9480 9626 387 Size: 1442 374 146 1280 Fragments arranged by size: 2188 1442 1280 1101 1064 1028 854 651 374 253 146 138 EcoICRI GAG'CTC Cuts at: 2719 3148 6363 2719 Size: 429 3215 6875 Fragments arranged by size: 6875 3215 429 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2234 2291 2474 2483 2618 2724 Size: 393 581 21 57 183 9 135 106 Cuts at: 2724 2870 2879 3305 3584 3593 3837 4037 4301 Size: 146 9 426 279 9 244 200 264 Cuts at: 4301 4401 4823 4877 4891 5413 5690 5889 6557 Size: 100 422 54 14 522 277 199 668 Cuts at: 6557 6602 6979 7145 8582 9179 9966 10234 1239 Size: 45 377 166 1437 597 787 268 1524 Fragments arranged by size: 1524 1437 787 668 597 581 522 426 422 393 377 279 277 268 264 244 200 199 183 166 146 135 106 100 57 54 45 21 14 9 9 9 EcoNI CCTnn'n_nnAGG Cuts at: 4205 4342 5921 7547 4205 Size: 137 1579 1626 7177 Fragments arranged by size: 7177 1626 1579 137 EcoO109I rG'GnC_Cy Cuts at: 2623 2935 3001 3399 3447 3798 3799 3973 4753 Size: 312 66 398 48 351 1 174 780 Cuts at: 4753 5129 5970 5971 5998 5999 6353 6512 6943 Size: 376 841 1 27 1 354 159 431 Cuts at: 6943 7021 7250 7291 2623 Size: 78 229 41 5851 Fragments arranged by size: 5851 841 780 431 398 376 354 351 312 229 174 159 78 66 48 41 27 1 1 1 EcoRI G'AATT_C Cuts at: 7828 8206 7828 Size: 378 10141 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3310 4657 6490 6820 3310 Size: 1347 1833 330 7009 Fragments arranged by size: 7009 1833 1347 330 FspI TGC'GCA Cuts at: 3185 5156 7520 7751 9025 3185 Size: 1971 2364 231 1274 4679 Fragments arranged by size: 4679 2364 1971 1274 231 FspAI rTGC'GCAy Cuts at: 5156 5156 Size: 10519 HaeII r_GCGC'y Cuts at: 1678 2342 2639 2913 3727 6692 7504 7659 7801 Size: 664 297 274 814 2965 812 155 142 Cuts at: 7801 9666 1678 Size: 1865 2531 Fragments arranged by size: 2965 2531 1865 814 812 664 297 274 155 142 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3158 3182 3190 3214 3436 Size: 32 135 32 1586 24 8 24 222 Cuts at: 3436 3468 3502 3534 4667 4699 5407 5439 5529 Size: 32 34 32 1133 32 708 32 90 Cuts at: 5529 5561 7445 7477 7487 7519 7572 7604 7607 Size: 32 1884 32 10 32 53 32 3 Cuts at: 7607 7639 7712 7744 9160 9192 9234 9266 1373 Size: 32 73 32 1416 32 42 32 2626 Fragments arranged by size: 2626 1884 1586 1416 1133 708 222 135 90 73 53 42 34 32 32 32 32 32 32 32 32 32 32 32 32 32 32 24 24 10 8 3 HincII GTy'rAC Cuts at: 669 1657 1938 5563 5698 7863 8109 669 Size: 988 281 3625 135 2165 246 3079 Fragments arranged by size: 3625 3079 2165 988 281 246 135 HindIII A'AGCT_T Cuts at: 748 6472 748 Size: 5724 4795 HpaI GTT'AAC Cuts at: 8109 8109 Size: 10519 KasI G'GCGC_C Cuts at: 2635 7655 2635 Size: 5020 5499 KpnI G_GTAC'C Cuts at: 4902 7844 4902 Size: 2942 7577 MfeI C'AATT_G Cuts at: 8118 8118 Size: 10519 MluI A'CGCG_T Cuts at: 10395 10395 Size: 10519 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2811 4125 5253 5526 5670 9633 9817 1305 Size: 1506 1314 1128 273 144 3963 184 2007 Fragments arranged by size: 3963 2007 1506 1314 1128 273 184 144 MscI TGG'CCA Cuts at: 10 64 1489 1832 2023 3770 3833 4652 4929 Size: 54 1425 343 191 1747 63 819 277 Cuts at: 4929 5913 7559 7715 10 Size: 984 1646 156 2814 Fragments arranged by size: 2814 1747 1646 1425 984 819 343 277 191 156 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2528 3485 3545 5150 5178 5441 Size: 648 1080 282 957 60 1605 28 263 Cuts at: 5441 5670 6173 6257 6294 6639 8477 8836 8995 Size: 229 503 84 37 345 1838 359 159 Cuts at: 8995 518 Size: 2042 Fragments arranged by size: 2042 1838 1605 1080 957 648 503 359 345 282 263 229 159 84 60 37 28 MspA1I CmG'CkG Cuts at: 1606 2212 2395 2689 2766 2848 3016 3230 3493 Size: 606 183 294 77 82 168 214 263 Cuts at: 3493 3562 4434 4742 5008 5053 5411 5670 6154 Size: 69 872 308 266 45 358 259 484 Cuts at: 6154 6375 6426 6460 6497 6550 6857 7225 7439 Size: 221 51 34 37 53 307 368 214 Cuts at: 7439 7681 7935 8612 9760 10005 1606 Size: 242 254 677 1148 245 2120 Fragments arranged by size: 2120 1148 872 677 606 484 368 358 308 307 294 266 263 259 254 245 242 221 214 214 183 168 82 77 69 53 51 45 37 34 NaeI GCC'GGC Cuts at: 3206 3527 3859 3882 5773 6823 3206 Size: 321 332 23 1891 1050 6902 Fragments arranged by size: 6902 1891 1050 332 321 23 NarI GG'CG_CC Cuts at: 2636 7656 2636 Size: 5020 5499 NcoI C'CATG_G Cuts at: 513 1065 2024 3105 7042 513 Size: 552 959 1081 3937 3990 Fragments arranged by size: 3990 3937 1081 959 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10519 NgoMIV G'CCGG_C Cuts at: 3204 3525 3857 3880 5771 6821 3204 Size: 321 332 23 1891 1050 6902 Fragments arranged by size: 6902 1891 1050 332 321 23 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10519 NotI GC'GGCC_GC Cuts at: 7966 7966 Size: 10519 NsiI A_TGCA'T Cuts at: 2378 2378 Size: 10519 NspI r_CATG'y Cuts at: 1132 1229 5140 6676 6698 7750 7877 9422 1132 Size: 97 3911 1536 22 1052 127 1545 2229 Fragments arranged by size: 3911 2229 1545 1536 1052 127 97 22 PasI CC'CwG_GG Cuts at: 1348 2259 3978 6842 1348 Size: 911 1719 2864 5025 Fragments arranged by size: 5025 2864 1719 911 PciI A'CATG_T Cuts at: 5136 9418 5136 Size: 4282 6237 PflMI CCAn_nnn'nTGG Cuts at: 1108 4213 4940 5236 5272 6535 6953 1108 Size: 3105 727 296 36 1263 418 4674 Fragments arranged by size: 4674 3105 1263 727 418 296 36 PfoI T'CCnGG_A Cuts at: 2517 3255 5865 7287 2517 Size: 738 2610 1422 5749 Fragments arranged by size: 5749 2610 1422 738 PmeI GTTT'AAAC Cuts at: 7823 7823 Size: 10519 PmlI CAC'GTG Cuts at: 5662 5662 Size: 10519 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 8588 8620 10101 10133 4019 Size: 32 4537 32 1481 32 4405 Fragments arranged by size: 4537 4405 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2623 3001 3973 5129 6353 7021 7291 2623 Size: 378 972 1156 1224 668 270 5851 Fragments arranged by size: 5851 1224 1156 972 668 378 270 PshAI GACnn'nnGTC Cuts at: 1613 6550 1613 Size: 4937 5582 PsiI TTA'TAA Cuts at: 8089 8089 Size: 10519 PspOMI G'GGCC_C Cuts at: 3398 3798 5970 5998 7249 3398 Size: 400 2172 28 1251 6668 Fragments arranged by size: 6668 2172 1251 400 28 PspXI vC'TCGA_Gb Cuts at: 6876 6876 Size: 10519 PstI C_TGCA'G Cuts at: 830 2394 2688 3021 4439 4546 7206 7353 7494 Size: 1564 294 333 1418 107 2660 147 141 Cuts at: 7494 7871 830 Size: 377 3478 Fragments arranged by size: 3478 2660 1564 1418 377 333 294 147 141 107 PvuI CG_AT'CG Cuts at: 2001 6204 2001 Size: 4203 6316 PvuII CAG'CTG Cuts at: 2395 2689 2766 2848 3016 3230 3493 3562 5053 Size: 294 77 82 168 214 263 69 1491 Cuts at: 5053 5670 6154 6460 7225 7439 7681 2395 Size: 617 484 306 765 214 242 5233 Fragments arranged by size: 5233 1491 765 617 484 306 294 263 242 214 214 168 82 77 69 SacI G_AGCT'C Cuts at: 2721 3150 6365 2721 Size: 429 3215 6875 Fragments arranged by size: 6875 3215 429 SacII CC_GC'GG Cuts at: 5412 5412 Size: 10519 SalI G'TCGA_C Cuts at: 1936 7861 1936 Size: 5925 4594 SanDI GG'GwC_CC Cuts at: 2623 3973 5129 2623 Size: 1350 1156 8013 Fragments arranged by size: 8013 1350 1156 SapI GCTCTTCn'nnn_ Cuts at: 2727 7280 2727 Size: 4553 5966 SbfI CC_TGCA'GG Cuts at: 7494 7871 7494 Size: 377 10142 ScaI AGT'ACT Cuts at: 2603 4196 4922 8767 10479 2603 Size: 1593 726 3845 1712 2643 Fragments arranged by size: 3845 2643 1712 1593 726 SexAI A'CCwGG_T Cuts at: 7065 7065 Size: 10519 SfcI C'TryA_G Cuts at: 826 1045 2390 2684 3017 3457 4435 4542 5371 Size: 219 1345 294 333 440 978 107 829 Cuts at: 5371 7202 7349 7490 7867 9002 9683 9874 826 Size: 1831 147 141 377 1135 681 191 1471 Fragments arranged by size: 1831 1471 1345 1135 978 829 681 440 377 333 294 219 191 147 141 107 SfiI GGCCn_nnn'nGGCC Cuts at: 5115 7721 5115 Size: 2606 7913 SfoI GGC'GCC Cuts at: 2637 7657 2637 Size: 5020 5499 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10519 SgrAI Cr'CCGG_yG Cuts at: 1700 2641 1700 Size: 941 9578 SmaI CCC'GGG Cuts at: 1872 3590 3923 7846 1872 Size: 1718 333 3923 4545 Fragments arranged by size: 4545 3923 1718 333 SmlI C'TyrA_G Cuts at: 820 1017 1943 6794 6876 7790 8623 9524 9786 Size: 197 926 4851 82 914 833 901 262 Cuts at: 9786 820 Size: 1553 Fragments arranged by size: 4851 1553 926 914 901 833 262 197 82 SnaBI TAC'GTA Cuts at: 493 5713 8310 493 Size: 5220 2597 2702 Fragments arranged by size: 5220 2702 2597 SpeI A'CTAG_T Cuts at: 152 6809 152 Size: 6657 3862 SphI G_CATG'C Cuts at: 1132 7750 7877 1132 Size: 6618 127 3774 Fragments arranged by size: 6618 3774 127 SspI AAT'ATT Cuts at: 5 52 8443 5 Size: 47 8391 2081 Fragments arranged by size: 8391 2081 47 StuI AGG'CCT Cuts at: 1517 2813 3653 7565 1517 Size: 1296 840 3912 4471 Fragments arranged by size: 4471 3912 1296 840 StyI C'CwwG_G Cuts at: 513 1065 2024 3105 3294 3390 4833 7042 7279 Size: 552 959 1081 189 96 1443 2209 237 Cuts at: 7279 7560 7958 513 Size: 281 398 3074 Fragments arranged by size: 3074 2209 1443 1081 959 552 398 281 237 189 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3240 4094 5105 6707 7151 8546 8563 Size: 1255 1660 854 1011 1602 444 1395 17 Cuts at: 8563 8716 8901 9346 325 Size: 153 185 445 1498 Fragments arranged by size: 1660 1602 1498 1395 1255 1011 854 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 2601 3609 Size: 274 80 33 51 1443 153 471 1008 Cuts at: 3609 4194 4920 7317 8765 10477 96 Size: 585 726 2397 1448 1712 138 Fragments arranged by size: 2397 1712 1448 1443 1008 726 585 471 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3566 3598 5126 5158 5339 5370 5371 5402 6950 Size: 32 1528 32 181 31 1 31 1548 Cuts at: 6950 6982 7283 7315 10479 10511 3566 Size: 32 301 32 3164 32 3574 Fragments arranged by size: 3574 3164 1548 1528 301 181 32 32 32 32 32 31 31 1 Tth111I GACn'n_nGTC Cuts at: 1316 2525 1316 Size: 1209 9310 XbaI T'CTAG_A Cuts at: 7855 7855 Size: 10519 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2833 3253 4937 5906 6256 6532 6712 6895 7615 Size: 420 1684 969 350 276 180 183 720 Cuts at: 7615 2833 Size: 5737 Fragments arranged by size: 5737 1684 969 720 420 350 276 183 180 XhoI C'TCGA_G Cuts at: 1943 6876 1943 Size: 4933 5586 XmaI C'CCGG_G Cuts at: 1870 3588 3921 7844 1870 Size: 1718 333 3923 4545 Fragments arranged by size: 4545 3923 1718 333 XmnI GAAnn'nnTTC Cuts at: 5189 8648 5189 Size: 3459 7060 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3327 8327 276 Size: 53 83 186 936 1793 5000 2468 Fragments arranged by size: 5000 2468 1793 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I EcoRV FseI NruI PacI PsrI RsrII SrfI SwaI