(Circular) (Six-base) MAPSORT of: pFN21AA0377.seq Check: 921 from: 1 to: 8923 pFN21AA0377 8923 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6733 278 Size: 53 83 186 936 5197 2468 Fragments arranged by size: 5197 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2786 4101 6266 1937 Size: 849 1315 2165 4594 Fragments arranged by size: 4594 2165 1315 849 Acc65I G'GTAC_C Cuts at: 6244 6244 Size: 8923 AclI AA'CG_TT Cuts at: 1563 7050 7423 1563 Size: 5487 373 3063 Fragments arranged by size: 5487 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2835 3443 3587 4702 5488 5559 6060 6986 Size: 1203 608 144 1115 786 71 501 926 Cuts at: 6986 8370 8638 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1203 1115 926 786 608 501 268 144 71 AfeI AGC'GCT Cuts at: 5282 5282 Size: 8923 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8726 AflIII A'CryG_T Cuts at: 1203 4496 5530 7822 8799 1203 Size: 3293 1034 2292 977 1327 Fragments arranged by size: 3293 2292 1327 1034 977 AgeI A'CCGG_T Cuts at: 7754 7754 Size: 8923 AhdI GACnn_n'nnGTC Cuts at: 4093 7652 4093 Size: 3559 5364 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8923 AlwNI CAG_nnn'CTG Cuts at: 6666 8238 6666 Size: 1572 7351 ApaI G_GGCC'C Cuts at: 4904 4904 Size: 8923 ApaLI G'TGCA_C Cuts at: 6980 8136 6980 Size: 1156 7767 ApoI r'AATT_y Cuts at: 1493 5132 5524 6232 6458 6610 8719 1493 Size: 3639 392 708 226 152 2109 1697 Fragments arranged by size: 3639 2109 1697 708 392 226 152 AseI AT'TA_AT Cuts at: 160 1032 7477 160 Size: 872 6445 1606 Fragments arranged by size: 6445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8923 AvaI C'yCGr_G Cuts at: 1870 1943 4461 4981 6248 1870 Size: 73 2518 520 1267 4545 Fragments arranged by size: 4545 2518 1267 520 73 AvrII C'CTAG_G Cuts at: 5047 5047 Size: 8923 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3554 3587 3554 Size: 33 8890 BamHI G'GATC_C Cuts at: 2454 4678 6253 6620 2454 Size: 2224 1575 367 4757 Fragments arranged by size: 4757 2224 1575 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 5071 5647 6244 7699 Size: 325 217 627 294 2990 576 597 1455 Cuts at: 7699 618 Size: 1842 Fragments arranged by size: 2990 1842 1455 627 597 576 325 294 217 BanII G_rGCy'C Cuts at: 1391 2127 4904 5346 5598 6242 1391 Size: 736 2777 442 252 644 4072 Fragments arranged by size: 4072 2777 736 644 442 252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2553 2619 3407 4370 4537 928 Size: 971 654 66 788 963 167 5314 Fragments arranged by size: 5314 971 963 788 654 167 66 BbvCI CC'TCA_GC Cuts at: 3129 3609 3129 Size: 480 8443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3032 3066 7114 7148 1903 Size: 34 1095 34 4048 34 3678 Fragments arranged by size: 4048 3678 1095 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6815 8025 6815 Size: 1210 7713 BclI T'GATC_A Cuts at: 1548 4030 5509 1548 Size: 2482 1479 4962 Fragments arranged by size: 4962 2482 1479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5325 7534 136 Size: 107 122 71 631 605 3653 2209 1525 Fragments arranged by size: 3653 2209 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3723 8918 3723 Size: 5195 3728 BlpI GC'TnA_GC Cuts at: 2644 5747 6340 2644 Size: 3103 593 5227 Fragments arranged by size: 5227 3103 593 Bme1580I G_kGCm'C Cuts at: 1790 2084 4759 4904 6984 8140 1790 Size: 294 2675 145 2080 1156 2573 Fragments arranged by size: 2675 2573 2080 1156 294 145 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6654 7612 442 Size: 416 53 83 399 24 5237 958 1753 Fragments arranged by size: 5237 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3555 4487 3555 Size: 932 7991 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2085 2670 2814 3311 3389 3455 3666 4181 Size: 846 585 144 497 78 66 211 515 Cuts at: 4181 5258 5549 5930 7583 1239 Size: 1077 291 381 1653 2579 Fragments arranged by size: 2579 1653 1077 846 585 515 497 381 291 211 144 78 66 Bpu10I CC'TnA_GC Cuts at: 3129 3318 3609 3129 Size: 189 291 8443 Fragments arranged by size: 8443 291 189 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2150 3293 3704 3895 5158 5338 7048 7913 8211 Size: 1143 411 191 1263 180 1710 865 298 Cuts at: 8211 2150 Size: 2862 Fragments arranged by size: 2862 1710 1263 1143 865 411 298 191 180 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 5564 5953 7586 882 Size: 617 3066 999 389 1633 2219 Fragments arranged by size: 3066 2219 1633 999 617 389 BsaAI yAC'GTr Cuts at: 493 1204 5246 5533 6714 493 Size: 711 4042 287 1181 2702 Fragments arranged by size: 4042 2702 1181 711 287 BsaBI GATnn'nnATC Cuts at: 4649 8917 4649 Size: 4268 4655 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6730 7112 7747 275 Size: 53 83 186 936 5197 382 635 1451 Fragments arranged by size: 5197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2451 3465 7356 7754 8028 8175 8586 2451 Size: 1014 3891 398 274 147 411 2788 Fragments arranged by size: 3891 2788 1014 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2265 2295 4820 4850 1178 Size: 30 165 30 862 30 2525 30 5251 Fragments arranged by size: 5251 2525 862 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2667 2762 3410 3626 4361 4814 4930 Size: 967 514 95 648 216 735 453 116 Cuts at: 4930 5693 5936 6068 6194 6227 1186 Size: 763 243 132 126 33 3882 Fragments arranged by size: 3882 967 763 735 648 514 453 243 216 132 126 116 95 33 BseYI C'CCAG_C Cuts at: 2077 2639 3616 4887 5387 5415 6034 8126 8622 Size: 562 977 1271 500 28 619 2092 496 Cuts at: 8622 2077 Size: 2378 Fragments arranged by size: 2378 2092 1271 977 619 562 500 496 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2628 3893 4262 1163 Size: 573 892 1265 369 5824 Fragments arranged by size: 5824 1265 892 573 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6373 7134 8162 1540 Size: 275 186 4372 761 1028 2301 Fragments arranged by size: 4372 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 2499 4473 5346 5590 5598 6984 7069 8140 Size: 372 1974 873 244 8 1386 85 1071 Cuts at: 8140 2127 Size: 2910 Fragments arranged by size: 2910 1974 1386 1071 873 372 244 85 8 BsmI GAATG_Cn' Cuts at: 2531 4086 6434 6527 2531 Size: 1555 2348 93 4927 Fragments arranged by size: 4927 2348 1555 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2672 3698 4295 4505 2672 Size: 1026 597 210 7090 Fragments arranged by size: 7090 1026 597 210 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2127 2499 4473 4759 4904 5346 Size: 399 294 43 372 1974 286 145 442 Cuts at: 5346 5590 5598 6242 6984 7069 8140 1391 Size: 244 8 644 742 85 1071 2174 Fragments arranged by size: 2174 1974 1071 742 644 442 399 372 294 286 244 145 85 43 8 BspEI T'CCGG_A Cuts at: 8586 8586 Size: 8923 BspHI T'CATG_A Cuts at: 6812 8542 6812 Size: 1730 7193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4113 4416 6278 844 Size: 3269 303 1862 3489 Fragments arranged by size: 3489 3269 1862 303 BsrBI CCG'CTC Cuts at: 1394 1823 6810 1394 Size: 429 4987 3507 Fragments arranged by size: 4987 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3186 4871 5792 7418 7592 65 Size: 1183 1938 1685 921 1626 174 1396 Fragments arranged by size: 1938 1685 1626 1396 1183 921 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4998 7567 7754 1700 Size: 110 3188 2569 187 2869 Fragments arranged by size: 3188 2869 2569 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8923 BssSI C'ACGA_G Cuts at: 4421 6983 7995 4421 Size: 2562 1012 5349 Fragments arranged by size: 5349 2562 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8923 BstBI TT'CG_AA Cuts at: 2386 4492 7727 2386 Size: 2106 3235 3582 Fragments arranged by size: 3582 3235 2106 BstEII G'GTnAC_C Cuts at: 1184 5810 1184 Size: 4626 4297 BstXI CCAn_nnnn'nTGG Cuts at: 3061 5387 5807 3061 Size: 2326 420 6177 Fragments arranged by size: 6177 2326 420 BstYI r'GATC_y Cuts at: 1972 2454 3523 3723 4068 4206 4678 6045 6253 Size: 482 1069 200 345 138 472 1367 208 Cuts at: 6253 6620 7005 7022 8474 8560 8572 8918 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1367 1069 482 472 385 367 346 345 208 200 138 86 17 12 BstZ17I GTA'TAC Cuts at: 2787 2787 Size: 8923 Bsu36I CC'TnA_GG Cuts at: 2520 3945 4896 6051 6177 6210 2520 Size: 1425 951 1155 126 33 5233 Fragments arranged by size: 5233 1425 1155 951 126 33 BtgI C'CryG_G Cuts at: 513 1065 2040 2086 2901 3107 3120 4953 5067 Size: 552 975 46 815 206 13 1833 114 Cuts at: 5067 513 Size: 4369 Fragments arranged by size: 4369 1833 975 815 552 206 114 46 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3876 487 Size: 683 56 2650 5534 Fragments arranged by size: 5534 2650 683 56 BtsI GCAGTG_nn' Cuts at: 2320 2701 4759 5096 5434 6439 7233 7253 2320 Size: 381 2058 337 338 1005 794 20 3990 Fragments arranged by size: 3990 2058 1005 794 381 338 337 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8888 DraI TTT'AAA Cuts at: 6227 6227 Size: 8923 DraIII CAC_nnn'GTG Cuts at: 3895 3895 Size: 8923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7930 809 Size: 507 6614 1802 Fragments arranged by size: 6614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2309 2412 Size: 54 1425 50 275 18 189 290 103 Cuts at: 2412 2916 3595 5018 5326 6370 7259 8888 8 Size: 504 679 1423 308 1044 889 1629 43 Fragments arranged by size: 1629 1425 1423 1044 889 679 504 308 290 275 189 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6370 1537 Size: 275 4558 4090 Fragments arranged by size: 4558 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2103 2460 3824 4175 4792 5256 6036 6853 Size: 750 357 1364 351 617 464 780 817 Cuts at: 6853 1353 Size: 3423 Fragments arranged by size: 3423 1364 817 780 750 617 464 357 351 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3698 7510 7884 8030 387 Size: 1064 138 2109 3812 374 146 1280 Fragments arranged by size: 3812 2109 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2125 5344 5596 2125 Size: 3219 252 5452 Fragments arranged by size: 5452 3219 252 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2670 2814 2835 3311 3389 3443 Size: 393 453 585 144 21 476 78 54 Cuts at: 3443 3455 3587 3666 4181 4702 5258 5488 5549 Size: 12 132 79 515 521 556 230 61 Cuts at: 5549 5559 5930 6060 6986 7583 8370 8638 1239 Size: 10 371 130 926 597 787 268 1524 Fragments arranged by size: 1524 926 787 597 585 556 521 515 476 453 393 371 268 230 144 132 130 79 78 61 54 21 12 10 EcoO109I rG'GnC_Cy Cuts at: 3778 4087 4900 4901 5704 5767 5830 6205 3778 Size: 309 813 1 803 63 63 375 6496 Fragments arranged by size: 6496 813 803 375 309 63 63 1 EcoRI G'AATT_C Cuts at: 5132 6232 6610 5132 Size: 1100 378 7445 Fragments arranged by size: 7445 1100 378 EcoRV GAT'ATC Cuts at: 2882 3343 4198 2882 Size: 461 855 7607 Fragments arranged by size: 7607 855 461 FspI TGC'GCA Cuts at: 7429 7429 Size: 8923 HaeII r_GCGC'y Cuts at: 1678 4192 5158 5284 5747 8070 1678 Size: 2514 966 126 463 2323 2531 Fragments arranged by size: 2531 2514 2323 966 463 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2866 2871 2898 2903 3430 Size: 32 135 32 1294 5 27 5 527 Cuts at: 3430 3462 4079 4111 4415 4447 4670 4702 4911 Size: 32 617 32 304 32 223 32 209 Cuts at: 4911 4943 4961 4993 5474 5501 5506 5533 6146 Size: 32 18 32 481 27 5 27 613 Cuts at: 6146 6178 7564 7596 7638 7670 1373 Size: 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1386 1294 617 613 527 481 304 223 209 135 42 32 32 32 32 32 32 32 32 32 32 32 27 27 27 18 5 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 4438 6267 6513 669 Size: 988 281 2500 1829 246 3079 Fragments arranged by size: 3079 2500 1829 988 281 246 HindIII A'AGCT_T Cuts at: 748 2381 3504 748 Size: 1633 1123 6167 Fragments arranged by size: 6167 1633 1123 HpaI GTT'AAC Cuts at: 6513 6513 Size: 8923 KpnI G_GTAC'C Cuts at: 6248 6248 Size: 8923 MfeI C'AATT_G Cuts at: 3476 6522 3476 Size: 3046 5877 MluI A'CGCG_T Cuts at: 4496 8799 4496 Size: 4303 4620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4130 8037 8221 1305 Size: 2825 3907 184 2007 Fragments arranged by size: 3907 2825 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2311 2414 2918 5328 10 Size: 54 1425 343 479 103 504 2410 3605 Fragments arranged by size: 3605 2410 1425 504 479 343 103 54 MslI CAynn'nnrTG Cuts at: 518 1166 3074 3167 3815 4425 5030 5163 5805 Size: 648 1908 93 648 610 605 133 642 Cuts at: 5805 6881 7240 7399 518 Size: 1076 359 159 2042 Fragments arranged by size: 2042 1908 1076 648 648 642 610 605 359 159 133 93 MspA1I CmG'CkG Cuts at: 1606 2648 3421 3882 3961 6038 6339 7016 8164 Size: 1042 773 461 79 2077 301 677 1148 Cuts at: 8164 8409 1606 Size: 245 2120 Fragments arranged by size: 2120 2077 1148 1042 773 677 461 301 245 79 NcoI C'CATG_G Cuts at: 513 1065 2901 3107 4953 5067 513 Size: 552 1836 206 1846 114 4369 Fragments arranged by size: 4369 1846 1836 552 206 114 NdeI CA'TA_TG Cuts at: 387 387 Size: 8923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8923 NotI GC'GGCC_GC Cuts at: 6370 6370 Size: 8923 NspI r_CATG'y Cuts at: 1132 1229 3640 5823 6281 7826 1132 Size: 97 2411 2183 458 1545 2229 Fragments arranged by size: 2411 2229 2183 1545 458 97 PasI CC'CwG_GG Cuts at: 1348 5576 1348 Size: 4228 4695 PciI A'CATG_T Cuts at: 7822 7822 Size: 8923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2765 1108 Size: 1657 7266 PfoI T'CCnGG_A Cuts at: 3327 4071 4840 5565 6006 3327 Size: 744 769 725 441 6244 Fragments arranged by size: 6244 769 744 725 441 PmeI GTTT'AAAC Cuts at: 6227 6227 Size: 8923 PmlI CAC'GTG Cuts at: 5533 5533 Size: 8923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4682 4714 6992 7024 8505 8537 4682 Size: 32 2278 32 1481 32 5068 Fragments arranged by size: 5068 2278 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4087 5704 5767 5830 6205 4087 Size: 1617 63 63 375 6805 Fragments arranged by size: 6805 1617 375 63 63 PshAI GACnn'nnGTC Cuts at: 1613 2261 1613 Size: 648 8275 PsiI TTA'TAA Cuts at: 6493 6493 Size: 8923 PspOMI G'GGCC_C Cuts at: 4900 4900 Size: 8923 PspXI vC'TCGA_Gb Cuts at: 4981 4981 Size: 8923 PstI C_TGCA'G Cuts at: 830 2243 2463 4110 4413 5188 6195 6275 830 Size: 1413 220 1647 303 775 1007 80 3478 Fragments arranged by size: 3478 1647 1413 1007 775 303 220 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8923 PvuII CAG'CTG Cuts at: 2648 3421 3882 3961 6038 2648 Size: 773 461 79 2077 5533 Fragments arranged by size: 5533 2077 773 461 79 SacI G_AGCT'C Cuts at: 2127 5346 5598 2127 Size: 3219 252 5452 Fragments arranged by size: 5452 3219 252 SalI G'TCGA_C Cuts at: 1936 6265 1936 Size: 4329 4594 SbfI CC_TGCA'GG Cuts at: 2463 6275 2463 Size: 3812 5111 ScaI AGT'ACT Cuts at: 3979 7171 8883 3979 Size: 3192 1712 4019 Fragments arranged by size: 4019 3192 1712 SexAI A'CCwGG_T Cuts at: 3379 3379 Size: 8923 SfcI C'TryA_G Cuts at: 826 1045 2239 2459 3920 4098 4106 4142 4409 Size: 219 1194 220 1461 178 8 36 267 Cuts at: 4409 5184 5475 6191 6271 7406 8087 8278 826 Size: 775 291 716 80 1135 681 191 1471 Fragments arranged by size: 1471 1461 1194 1135 775 716 681 291 267 220 219 191 178 80 36 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8923 SmaI CCC'GGG Cuts at: 1872 6250 1872 Size: 4378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 3308 3719 3874 4981 5137 Size: 197 926 186 1179 411 155 1107 156 Cuts at: 5137 5353 7027 7928 8190 820 Size: 216 1674 901 262 1553 Fragments arranged by size: 1674 1553 1179 1107 926 901 411 262 216 197 186 156 155 SnaBI TAC'GTA Cuts at: 493 6714 493 Size: 6221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8923 SphI G_CATG'C Cuts at: 1132 6281 1132 Size: 5149 3774 SspI AAT'ATT Cuts at: 5 52 6847 5 Size: 47 6795 2081 Fragments arranged by size: 6795 2081 47 StuI AGG'CCT Cuts at: 1517 4987 5219 5553 1517 Size: 3470 232 334 4887 Fragments arranged by size: 4887 3470 334 232 StyI C'CwwG_G Cuts at: 513 1065 2056 2901 3107 3251 3774 4816 4953 Size: 552 991 845 206 144 523 1042 137 Cuts at: 4953 5047 5067 5297 5554 6362 513 Size: 94 20 230 257 808 3074 Fragments arranged by size: 3074 1042 991 845 808 552 523 257 230 206 144 137 94 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3264 3823 4076 5038 5429 6950 6967 Size: 1255 1684 559 253 962 391 1521 17 Cuts at: 6967 7120 7305 7750 325 Size: 153 185 445 1498 Fragments arranged by size: 1684 1521 1498 1255 962 559 445 391 253 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3430 3977 4455 Size: 274 80 33 51 1443 1453 547 478 Cuts at: 4455 7169 8881 96 Size: 2714 1712 138 Fragments arranged by size: 2714 1712 1453 1443 547 478 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4663 4695 4820 4852 8883 8915 4663 Size: 32 125 32 4031 32 4671 Fragments arranged by size: 4671 4031 125 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2731 4226 1316 Size: 1415 1495 6013 Fragments arranged by size: 6013 1495 1415 XbaI T'CTAG_A Cuts at: 3790 6259 3790 Size: 2469 6454 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2054 2587 2908 3781 5086 5573 5990 2054 Size: 533 321 873 1305 487 417 4987 Fragments arranged by size: 4987 1305 873 533 487 417 321 XhoI C'TCGA_G Cuts at: 1943 4981 1943 Size: 3038 5885 XmaI C'CCGG_G Cuts at: 1870 6248 1870 Size: 4378 4545 XmnI GAAnn'nnTTC Cuts at: 3979 7052 3979 Size: 3073 5850 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6731 276 Size: 53 83 186 936 5197 2468 Fragments arranged by size: 5197 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BbeI BfrBI BsiWI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI