Restriction map for pFN21AA0363
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        ApoI        AscI        
AseI        AsnI        AviII       AvrII       BbeI        
BbrPI       BciVI       BclI        BfrI        BlnI        
BsaAI       BsaWI       BseAI       BsiWI       BspDI       
BspEI       BspHI       BspLU11I    BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DrdI        EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KasI        KspI        
MfeI        MluI        MroI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       3418  GGTACC               G^GTACC
AcyI          307  GACGCC               G[GA]^CG[CT]C
AhdI          784  GACAGCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4147  GTGCAC               G^TGCAC
Aor51HI       132  AGCGCT               AGC^GCT
ApaLI        4147  GTGCAC               G^TGCAC
Asp700I      1351  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3418  GGTACC               G^GTACC
AspEI         784  GACAGCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        4147  GTGCAC               G[AT]GC[AT]^C
BamHI         741  GGATCC               G^GATCC
BfaI         2858  CTAG                 C^TAG
BsaBI        4023  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         307  GACGCC               G[GA]^CG[CT]C
BsiEI         386  CGGCCG               CG[GA][CT]^CG
BsiHKAI      4147  GTGCAC               G[AT]GC[AT]^C
BsmBI          91  GAGACG               CGTCTC(1/5)
Bsp1407I      932  TGTACA               T^GTACA
BspMI        1185  GCAGGT               ACCTGC(4/8)
BsrBI         999  GAGCGG               CCGCTC(-3/-3)
BsrFI        2817  GCCGGC               [GA]^CCGG[CT]
BsrGI         932  TGTACA               T^GTACA
Cfr10I       2817  GCCGGC               [GA]^CCGG[CT]
DraIII       1386  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          386  CGGCCG               C^GGCCG
Eam1105I      784  GACAGCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         386  CGGCCG               C^GGCCG
Eco47III      132  AGCGCT               AGC^GCT
Eco52I        386  CGGCCG               C^GGCCG
HaeII         132  AGCGCT               [GA]GCGC^[CT]
Hin1I         307  GACGCC               G[GA]^CG[CT]C
HincII       4381  GTCAAC               GT[CT]^[GA]AC
HindII       4381  GTCAAC               GT[CT]^[GA]AC
KpnI         3418  GGTACC               GGTAC^C
MaeI         2858  CTAG                 C^TAG
MamI         4023  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         4172  TTAA                 T^TAA
NaeI         2817  GCCGGC               GCC^GGC
NgoMIV       2817  GCCGGC               G^CCGGC
NspI         2875  GCATGT               [GA]CATG^[CT]
PflMI        1231  CCACAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        2996  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI        2708  ACCAGGT              A^CC[AT]GGT
SmaI         4223  CCCGGG               CCC^GGG
SmlI         3740  CTCAAG               C^T[CT][GA]AG
SspBI         932  TGTACA               T^GTACA
TaqI          605  TCGA                 T^CGA
Tru9I        4172  TTAA                 T^TAA
Tsp509I      1109  AATT                 ^AATT
TthHB8I       605  TCGA                 T^CGA
Van91I       1231  CCACAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        4223  CCCGGG               C^CCGGG
XmaI         4223  CCCGGG               C^CCGGG
XmnI         1351  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BcgI          846  CGAGGCTTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              867  CGACACCTCTGC        
BsaI         2935  GAGACC               GGTCTC(1/5)
             3928  GAGACC              
BsmI         2667  GAATGC               GAATGC(1/-1)
             3822  GCATTC              
BsrDI        1288  GCAATG               GCAATG(2/0)
             3979  GCAATG              
BstAPI       2575  GCAGGCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3681  GCAGATCTTGC         
BstXI         144  CCAGGAGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3665  CCAAAGACCTGG        
HgaI          307  GACGC                GACGC(5/10)
             3226  GACGC               
SfiI          586  GGCCAGGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3150  GGCCACAGAGGCC       


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AspI        AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflFI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StuI        StyI        
TfiI        TseI        Tsp45I      TspRI       Tth111I     
XcmI        XhoII       
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