Restriction map for
pFN21AA0358
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# NO CUT ENZYMES ( 57 enzymes) #
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AccIII AclI AflII AgeI ApaI
AscI AviII BbrPI BciVI BfrI
BglII BsaAI BseAI BsiEI BsiWI
BspEI BssSI BstBI CpoI EagI
EclXI Eco52I EcoT22I FseI FspI
KspI MfeI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
Van91I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 4052 GGTACC G^GTACC
Alw44I 2845 GTGCAC G^TGCAC
Aor51HI 4427 AGCGCT AGC^GCT
ApaLI 2845 GTGCAC G^TGCAC
AseI 1948 ATTAAT AT^TAAT
AsnI 1948 ATTAAT AT^TAAT
Asp718I 4052 GGTACC G^GTACC
AvrII 3864 CCTAGG C^CTAGG
BbeI 4190 GGCGCC GGCGC^C
BcgI 723 GCATGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 617 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3864 CCTAGG C^CTAGG
BlpI 537 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 537 GCTCAGC GC^T[ACGT]AGC
BsaBI 4096 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4076 ACCGGA [AT]^CCGG[AT]
BspDI 755 ATCGAT AT^CGAT
BspHI 1344 TCATGA T^CATGA
BspLU11I 2394 ACATGT A^CATGT
BssHII 4581 GCGCGC G^CGCGC
Bst1107I 579 GTATAC GTA^TAC
BstEII 4680 GGTGACC G^GT[ACGT]ACC
BstPI 4680 GGTGACC G^GT[ACGT]ACC
BstZ17I 579 GTATAC GTA^TAC
CelII 537 GCTCAGC GC^T[ACGT]AGC
ClaI 755 ATCGAT AT^CGAT
Eco47III 4427 AGCGCT AGC^GCT
EcoO65I 4680 GGTGACC G^GT[ACGT]ACC
EcoRV 4360 GATATC GAT^ATC
HincII 283 GTTAAC GT[CT]^[GA]AC
HindII 283 GTTAAC GT[CT]^[GA]AC
HindIII 3398 AAGCTT A^AGCTT
HpaI 283 GTTAAC GTT^AAC
KasI 4190 GGCGCC G^GCGCC
KpnI 4052 GGTACC GGTAC^C
MamI 4096 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1528 ACGCGT A^CGCGT
NarI 4190 GGCGCC GG^CGCC
NcoI 1475 CCATGG C^CATGG
PaeR7I 644 CTCGAG C^TCGAG
PshBI 1948 ATTAAT AT^TAAT
RcaI 1344 TCATGA T^CATGA
SexAI 3666 ACCAGGT A^CC[AT]GGT
SfoI 4190 GGCGCC GGC^GCC
SgrAI 2290 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2698 CCCGGG CCC^GGG
XhoI 644 CTCGAG C^TCGAG
XmaCI 2698 CCCGGG C^CCGGG
XmaI 2698 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AatII 2258 GACGTC GACGT^C
3501 GACGTC
AccI 579 GTATAC GT^[AC][GT]AC
799 GTAGAC
AflIII 1528 ACGCGT A^C[GA][CT]GT
2394 ACATGT
AhdI 1390 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4458 GACCTACTGTC
AocI 2341 CCTGAGG CC^T[ACGT]AGG
3307 CCTAAGG
Asp700I 12 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1376 GAAGGCCTTC
AspEI 1390 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4458 GACCTACTGTC
AspI 1918 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3529 GACTCTGTC
BbsI 4653 GAAGAC GAAGAC(2/6)
232 GTCTTC
BbvCI 3057 CCTCAGC CCTCAGC(-5/-2)
1804 GCTGAGG
BclI 3522 TGATCA T^GATCA
4251 TGATCA
BpuAI 4653 GAAGAC GAAGAC(2/6)
232 GTCTTC
BsmI 944 GCATTC GAATGC(1/-1)
1012 GCATTC
BsrDI 1021 GCAATG GCAATG(2/0)
3650 GCAATG
BstAPI 2847 GCACAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3283 GCACCAAGTGC
Bsu36I 2341 CCTGAGG CC^T[ACGT]AGG
3307 CCTAAGG
DraI 1281 TTTAAA TTT^AAA
3330 TTTAAA
DrdI 253 GACACTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
802 GACATCGAGGTC
Eam1105I 1390 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4458 GACCTACTGTC
Eco81I 2341 CCTGAGG CC^T[ACGT]AGG
3307 CCTAAGG
EcoNI 2832 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4671 CCTGCTGCAGG
EcoRI 1414 GAATTC G^AATTC
1694 GAATTC
FbaI 3522 TGATCA T^GATCA
4251 TGATCA
PflFI 1918 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3529 GACTCTGTC
SacI 402 GAGCTC GAGCT^C
823 GAGCTC
ScaI 514 AGTACT AGT^ACT
1646 AGTACT
Tth111I 1918 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3529 GACTCTGTC
XmnI 12 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1376 GAAGGCCTTC
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# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PshAI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII